P32917
Gene name |
STE5 (NUL3, YDR103W, YD8557.12) |
Protein name |
Protein STE5 |
Names |
|
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YDR103W |
EC number |
|
Protein Class |
|
Descriptions
The yeast mating mitogen-activated protein kinase (MAPK) pathway is stimulated by the scaffold protein Ste5. The autoinhibition of Ste5 is controlled by an intramolecular interaction of the pleckstrin homology (PH) domain and the von Willebrand type A (VWA) domain. The interaction blocks the ability to co-activate the mating-specific MAPK Fus3. Pheromone-induced membrane binding of Ste5 triggers release of this autoinhibition. Relief of autoinhibition on VWA domain by anti-PH Fab antibodies (SR13), which binds to the PH domain, permits Fus3 activation.
Autoinhibitory domains (AIDs)
Target domain |
593-786 (VWA domain) |
Relief mechanism |
Ligand binding |
Assay |
Split protein assay, Deletion assay, Structural analysis |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
5 structures for P32917
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 2KGN | NMR | - | A | 44-67 | PDB |
| 2L4U | NMR | - | A | 44-67 | PDB |
| 3FZE | X-ray | 160 A | A | 593-786 | PDB |
| 4F2H | X-ray | 319 A | A | 583-787 | PDB |
| AF-P32917-F1 | Predicted | AlphaFoldDB |
23 variants for P32917
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s04-658417 | 24 | G>D | No | SGRP | |
| s04-658753 | 136 | L>R | No | SGRP | |
| s04-659039 | 231 | K>N | No | SGRP | |
| s04-659335 | 330 | P>L | No | SGRP | |
| s04-659343 | 333 | H>N | No | SGRP | |
| s04-659464 | 373 | T>I | No | SGRP | |
| s04-659686 | 447 | I>T | No | SGRP | |
| s04-659967 | 541 | H>Y | No | SGRP | |
| s04-660022 | 559 | G>A | No | SGRP | |
| s04-660091 | 582 | R>K | No | SGRP | |
| s04-660150 | 602 | L>F | No | SGRP | |
| s04-660234 | 630 | V>I | No | SGRP | |
| s04-660328 | 661 | V>A | No | SGRP | |
| s04-660335 | 663 | H>Q | No | SGRP | |
| s04-660364 | 673 | N>T | No | SGRP | |
| s04-660387 | 681 | T>S | No | SGRP | |
| s04-660495 | 717 | I>V | No | SGRP | |
| s04-660678 | 778 | H>D | No | SGRP | |
| s04-660745 | 800 | S>L | No | SGRP | |
| s04-660799 | 818 | N>S | No | SGRP | |
| s04-660933 | 863 | H>Y | No | SGRP | |
| s04-660941 | 865 | E>D | No | SGRP | |
| s04-660976 | 877 | D>G | No | SGRP |
No associated diseases with P32917
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| mating projection tip | The apex of the mating projection in unicellular fungi exposed to mating pheromone; site of polarized growth. |
| mating projection tip membrane | The portion of the plasma membrane surrounding a mating projection tip. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| G-protein beta-subunit binding | Binding to a G-protein beta subunit. |
| kinase binding | Binding to a kinase, any enzyme that catalyzes the transfer of a phosphate group. |
| MAP-kinase scaffold activity | The binding activity of a molecule that functions as a physical support for the assembly of a multiprotein mitogen-activated protein kinase (MAPK) complex. Binds multiple kinases of the MAPKKK cascade, and also upstream signaling proteins, permitting those molecules to function in a coordinated way. Bringing together multiple enzymes and their substrates enables the signal to be transduced quickly and efficiently. |
| phosphatidylinositol-4,5-bisphosphate binding | Binding to phosphatidylinositol-4,5-bisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 4' and 5' positions. |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| invasive growth in response to glucose limitation | A growth pattern exhibited by budding haploid cells under certain growth conditions, in which cells retain the typical axial budding pattern of haploids, but become elongated and fail to separate after division; during growth on a solid substrate, this results in penetration of cells into the agar medium. An example of this process is found in Saccharomyces cerevisiae. |
| negative regulation of MAPK cascade | Any process that stops, prevents, or reduces the frequency, rate or extent of signal transduction mediated by the MAPKKK cascade. |
| pheromone-dependent signal transduction involved in conjugation with cellular fusion | A signal transduction process resulting in the relay, amplification or dampening of a signal generated in response to pheromone exposure in organisms that undergo conjugation with cellular fusion. An example of this process is found in Saccharomyces cerevisiae. |
| positive regulation of protein phosphorylation | Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein. |
| regulation of transposition, RNA-mediated | Any process that modulates the frequency, rate or extent of RNA-mediated transposition. RNA-mediated transposition is a type of transpositional recombination which occurs via an RNA intermediate. |
No homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| No homologous proteins | ||||
| 10 | 20 | 30 | 40 | 50 | 60 |
| MMETPTDNIV | SPFHNFGSST | QYSGTLSRTP | NQIIELEKPS | TLSPLSRGKK | WTEKLARFQR |
| 70 | 80 | 90 | 100 | 110 | 120 |
| SSAKKKRFSP | SPISSSTFSF | SPKSRVTSSN | SSGNEDGNLM | NTPSTVSTDY | LPQHPHRTSS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LPRPNSNLFH | ASNSNLSRAN | EPPRAENLSD | NIPPKVAPFG | YPIQRTSIKK | SFLNASCTLC |
| 190 | 200 | 210 | 220 | 230 | 240 |
| DEPISNRRKG | EKIIELACGH | LSHQECLIIS | FGTTSKADVR | ALFPFCTKCK | KDTNKAVQCI |
| 250 | 260 | 270 | 280 | 290 | 300 |
| PENDELKDIL | ISDFLIHKIP | DSELSITPQS | RFPPYSPLLP | PFGLSYTPVE | RQTIYSQAPS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LNPNLILAAP | PKERNQIPQK | KSNYTFLHSP | LGHRRIPSGA | NSILADTSVA | LSANDSISAV |
| 370 | 380 | 390 | 400 | 410 | 420 |
| SNSVRAKDDE | TKTTLPLLRS | YFIQILLNNF | QEELQDWRID | GDYGLLRLVD | KLMISKDGQR |
| 430 | 440 | 450 | 460 | 470 | 480 |
| YIQCWCFLFE | DAFVIAEVDN | DVDVLEIRLK | NLEVFTPIAN | LRMTTLEASV | LKCTLNKQHC |
| 490 | 500 | 510 | 520 | 530 | 540 |
| ADLSDLYIVQ | NINSDESTTV | QKWISGILNQ | DFVFNEDNIT | STLPILPIIK | NFSKDVGNGR |
| 550 | 560 | 570 | 580 | 590 | 600 |
| HETSTFLGLI | NPNKVVEVGN | VHDNDTVIIR | RGFTLNSGEC | SRQSTVDSIQ | SVLTTISSIL |
| 610 | 620 | 630 | 640 | 650 | 660 |
| SLKREKPDNL | AIILQIDFTK | LKEEDSLIVV | YNSLKALTIK | FARLQFCFVD | RNNYVLDYGS |
| 670 | 680 | 690 | 700 | 710 | 720 |
| VLHKIDSLDS | ISNLKSKSSS | TQFSPIWLKN | TLYPENIHEH | LGIVAVSNSN | MEAKKSILFQ |
| 730 | 740 | 750 | 760 | 770 | 780 |
| DYRCFTSFGR | RRPNELKIKV | GYLNVDYSDK | IDELVEASSW | TFVLETLCYS | FGLSFDEHDD |
| 790 | 800 | 810 | 820 | 830 | 840 |
| DDEEDNDDST | DNELDNSSGS | LSDAESTTTI | HIDSPFDNEN | ATANMVNDRN | LLTEGEHSNI |
| 850 | 860 | 870 | 880 | 890 | 900 |
| ENLETVASSV | QPALIPNIRF | SLHSEEEGTN | ENENENDMPV | LLLSDMDKGI | DGITRRSSFS |
| 910 | |||||
| SLIESGNNNC | PLHMDYI |