P32865

PR
Gene name
Gprk1 (Gprk-1, CG40129)
Protein name
G protein-coupled receptor kinase 1
Names
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG40129
EC number
2.7.11.16: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

110-206 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P32865

Entry ID Method Resolution Chain Position Source
AF-P32865-F1 Predicted AlphaFoldDB

No variants for P32865

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P32865

No associated diseases with P32865

3 regional properties for P32865

Type Name Position InterPro Accession
domain Protein kinase domain 64 - 325 IPR000719
active_site Serine/threonine-protein kinase, active site 186 - 198 IPR008271
binding_site Protein kinase, ATP binding site 70 - 93 IPR017441

Functions

Description
EC Number 2.7.11.16 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
G protein-coupled receptor binding Binding to a G protein-coupled receptor.
G protein-coupled receptor kinase activity Catalysis of the reaction: ATP + G protein-coupled receptor = ADP + G protein-coupled receptor phosphate.
phosphatidylinositol binding Binding to an inositol-containing glycerophospholipid, i.e. phosphatidylinositol (PtdIns) and its phosphorylated derivatives.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
rhodopsin kinase activity Catalysis of the reaction: ATP + rhodopsin = ADP + phosphorhodopsin.

6 GO annotations of biological process

Name Definition
G protein-coupled receptor signaling pathway The series of molecular signals initiated by a ligand binding to its receptor, in which the activated receptor promotes the exchange of GDP for GTP on the alpha-subunit of an associated heterotrimeric G-protein complex. The GTP-bound activated alpha-G-protein then dissociates from the beta- and gamma-subunits to further transmit the signal within the cell. The pathway begins with receptor-ligand interaction, and ends with regulation of a downstream cellular process. The pathway can start from the plasma membrane, Golgi or nuclear membrane.
metarhodopsin inactivation The process in which metarhodopsin is prevented from generating molecular signals. Activated rhodopsin (R*) is inactivated by a two-step process: first, R* is phosphorylated by rhodopsin kinase which lowers the activity of R*. Second, the protein arrestin binds to phosphorylated R* to de-activate it.
phototransduction, visible light The sequence of reactions within a cell required to convert absorbed photons from visible light into a molecular signal. A visible light stimulus is electromagnetic radiation that can be perceived visually by an organism; for organisms lacking a visual system, this can be defined as light with a wavelength within the range 380 to 780 nm.
positive regulation of smoothened signaling pathway Any process that activates or increases the frequency, rate or extent of smoothened signaling.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of signal transduction Any process that modulates the frequency, rate or extent of signal transduction.

19 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P43249 GRK5 G protein-coupled receptor kinase 5 Bos taurus (Bovine) SS
P21146 GRK2 Beta-adrenergic receptor kinase 1 Bos taurus (Bovine) PR
P26818 GRK3 Beta-adrenergic receptor kinase 2 Bos taurus (Bovine) PR
Q15835 GRK1 Rhodopsin kinase GRK1 Homo sapiens (Human) PR
P34947 GRK5 G protein-coupled receptor kinase 5 Homo sapiens (Human) EV
P32298 GRK4 G protein-coupled receptor kinase 4 Homo sapiens (Human) SS
P43250 GRK6 G protein-coupled receptor kinase 6 Homo sapiens (Human) EV
P25098 GRK2 Beta-adrenergic receptor kinase 1 Homo sapiens (Human) PR
P35626 GRK3 Beta-adrenergic receptor kinase 2 Homo sapiens (Human) PR
O70293 Grk6 G protein-coupled receptor kinase 6 Mus musculus (Mouse) SS
Q8VEB1 Grk5 G protein-coupled receptor kinase 5 Mus musculus (Mouse) SS
Q9WVL4 Grk1 Rhodopsin kinase GRK1 Mus musculus (Mouse) PR
Q3UYH7 Adrbk2 Beta-adrenergic receptor kinase 2 Mus musculus (Mouse) PR
Q99MK8 Grk2 Beta-adrenergic receptor kinase 1 Mus musculus (Mouse) PR
Q62833 Grk5 G protein-coupled receptor kinase 5 Rattus norvegicus (Rat) SS
P97711 Grk6 G protein-coupled receptor kinase 6 Rattus norvegicus (Rat) SS
P26819 Grk3 Beta-adrenergic receptor kinase 2 Rattus norvegicus (Rat) PR
P26817 Grk2 Beta-adrenergic receptor kinase 1 Rattus norvegicus (Rat) PR
Q09639 grk-2 G protein-coupled receptor kinase 2 Caenorhabditis elegans PR
10 20 30 40 50 60
MADLEAVLAD VSYLMAMEKS KCTPAARASK KLNLPDPSVR SVMYKYLEKE GELNFHKIFN
70 80 90 100 110 120
EVLGYLLFKD FCENDSEEPI QQLKFFEQIK LFEKTECYDE RKKMARDIYD NFIMEEMLSH
130 140 150 160 170 180
TYEYSKHAVA SVQKYLLKNE VPVDLFEPYL EEIFTQLKGK PFKKFLESDK FTRFCQWKNL
190 200 210 220 230 240
ELNIQLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGEMLALNER
250 260 270 280 290 300
NMLQAVSTGI DCPFIVCMTY AFHTPDKLCF ILDLMNGGDL HYHLSQHGIF SEDEMKFYAA
310 320 330 340 350 360
EVILGLEHMH KRCIVYRDLK PANILLDENG HIRISDLGLA CDFSKKKPHA SVGTHGYMAP
370 380 390 400 410 420
EVLSKGTSYD SCADWFSFGC MLYKLLKGHS PFRQHKTKDK LEIDKMTLTM NVELPESFSL
430 440 450 460 470 480
ELKNLLEMLL QRDVSKRLGC MGNGADEVKM HNFFCGIDWH QVYIQKYTPP LVPPRGEVNA
490 500 510 520 530 540
ADAFDIGSFD EEDTKGIKLN DADQDLYKMF SLTISERWQQ EVSETVFDTV NTETDKLEQK
550 560 570 580 590 600
RKLKQKQHFD ADEKESDCIL HGYIKKLGGS FASLWQTKYA KLYPNRLELH SESGNNKPEL
610 620 630 640 650 660
IFMDQVEDIS SDFILHKNEN CIQIRINDGT RDGRIILTNS DEIGLKEWSS SLRSAHKISQ
670 680 690
DLLGSMAKKA GKIYGSERDV NKSMYIFGGN CSTKTSNGSN