P32660
PR
Gene name |
DNF1 (YER166W, SYGP-ORF7) |
Protein name |
Phospholipid-transporting ATPase DNF1 |
Names |
Flippase DNF1 |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YER166W |
EC number |
7.6.2.1: Linked to the hydrolysis of a nucleoside triphosphate |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
10 structures for P32660
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 7DRX | EM | 290 A | A | 1-1571 | PDB |
| 7DSH | EM | 367 A | A | 1-1571 | PDB |
| 7DSI | EM | 321 A | A | 1-1571 | PDB |
| 7F7F | EM | 381 A | A | 1-1571 | PDB |
| 7KY6 | EM | 310 A | A | 1-1571 | PDB |
| 7KYB | EM | 320 A | A | 1-1571 | PDB |
| 7KYC | EM | 280 A | A | 1-1571 | PDB |
| 7WHV | EM | 280 A | A | 1-1571 | PDB |
| 7WHW | EM | 310 A | A | 1-1571 | PDB |
| AF-P32660-F1 | Predicted | AlphaFoldDB |
9 variants for P32660
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s05-512926 | 63 | R>H | No | SGRP | |
| s05-512991 | 85 | T>S | No | SGRP | |
| s05-513787 | 350 | D>G | No | SGRP | |
| s05-513936 | 400 | G>S | No | SGRP | |
| s05-514318 | 527 | G>D | No | SGRP | |
| s05-515316 | 860 | P>T | No | SGRP | |
| s05-515392 | 885 | S>N | No | SGRP | |
| s05-516969 | 1411 | V>I | No | SGRP | |
| s05-517322 | 1528 | D>E | No | SGRP |
No associated diseases with P32660
No regional properties for P32660
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for P32660 | |||
Functions
| Description | ||
|---|---|---|
| EC Number | 7.6.2.1 | Linked to the hydrolysis of a nucleoside triphosphate |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
10 GO annotations of cellular component
| Name | Definition |
|---|---|
| cell periphery | The part of a cell encompassing the cell cortex, the plasma membrane, and any external encapsulating structures. |
| cellular bud neck | The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding. |
| endoplasmic reticulum | The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached). |
| endosome membrane | The lipid bilayer surrounding an endosome. |
| Golgi apparatus | A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways. |
| integral component of membrane | The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane. |
| mating projection tip membrane | The portion of the plasma membrane surrounding a mating projection tip. |
| mitochondrion | A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
| phospholipid-translocating ATPase complex | A protein complex that functions as a phospholipid-translocating P-Type ATPase. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
7 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. |
| ATPase-coupled intramembrane lipid transporter activity | Catalysis of the movement of lipids from one membrane leaflet to the other, driven by ATP hydrolysis. This includes flippases and floppases. |
| identical protein binding | Binding to an identical protein or proteins. |
| magnesium ion binding | Binding to a magnesium (Mg) ion. |
| phosphatidylcholine flippase activity | Catalysis of the movement of phosphatidylcholine from the exoplasmic to the cytosolic leaftlet of a membrane, using energy from the hydrolysis of ATP. |
| phosphatidylethanolamine flippase activity | Catalysis of the movement of phosphatidylethanolamine from the exoplasmic to the cytosolic leaftlet of a membrane, using energy from the hydrolysis of ATP. |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| endocytosis | A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a small region of the plasma membrane to form a new membrane-bounded vesicle. |
| establishment or maintenance of cell polarity | Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization or cell growth patterns. |
| intracellular protein transport | The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell. |
| phospholipid translocation | The movement of a phospholipid molecule from one leaflet of a membrane bilayer to the opposite leaflet. |
| response to pheromone triggering conjugation with cellular fusion | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a pheromone stimulus that positively regulates the process of conjugation with cellular fusion. An example of this process is found in Saccharomyces cerevisiae. |
2 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P39524 | DRS2 | Phospholipid-transporting ATPase DRS2 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | EV |
| P40527 | NEO1 | Probable phospholipid-transporting ATPase NEO1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSGTFHGDGH | APMSPFEDTF | QFEDNSSNED | THIAPTHFDD | GATSNKYSRP | QVSFNDETPK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| NKREDAEEFT | FNDDTEYDNH | SFQPTPKLNN | GSGTFDDVEL | DNDSGEPHTN | YDGMKRFRMG |
| 130 | 140 | 150 | 160 | 170 | 180 |
| TKRNKKGNPI | MGRSKTLKWA | RKNIPNPFED | FTKDDIDPGA | INRAQELRTV | YYNMPLPKDM |
| 190 | 200 | 210 | 220 | 230 | 240 |
| IDEEGNPIMQ | YPRNKIRTTK | YTPLTFLPKN | ILFQFHNFAN | VYFLVLIILG | AFQIFGVTNP |
| 250 | 260 | 270 | 280 | 290 | 300 |
| GLSAVPLVVI | VIITAIKDAI | EDSRRTVLDL | EVNNTKTHIL | EGVENENVST | DNISLWRRFK |
| 310 | 320 | 330 | 340 | 350 | 360 |
| KANSRLLFKF | IQYCKEHLTE | EGKKKRMQRK | RHELRVQKTV | GTSGPRSSLD | SIDSYRVSAD |
| 370 | 380 | 390 | 400 | 410 | 420 |
| YGRPSLDYDN | LEQGAGEANI | VDRSLPPRTD | CKFAKNYWKG | VKVGDIVRIH | NNDEIPADII |
| 430 | 440 | 450 | 460 | 470 | 480 |
| LLSTSDTDGA | CYVETKNLDG | ETNLKVRQSL | KCTNTIRTSK | DIARTKFWIE | SEGPHSNLYT |
| 490 | 500 | 510 | 520 | 530 | 540 |
| YQGNMKWRNL | ADGEIRNEPI | TINNVLLRGC | TLRNTKWAMG | VVMFTGGDTK | IMLNSGITPT |
| 550 | 560 | 570 | 580 | 590 | 600 |
| KKSRISRELN | FSVVINFVLL | FILCFVSGIA | NGVYYDKKGR | SRFSYEFGTI | AGSAATNGFV |
| 610 | 620 | 630 | 640 | 650 | 660 |
| SFWVAVILYQ | SLVPISLYIS | VEIIKTAQAA | FIYGDVLLYN | AKLDYPCTPK | SWNISDDLGQ |
| 670 | 680 | 690 | 700 | 710 | 720 |
| VEYIFSDKTG | TLTQNVMEFK | KCTINGVSYG | RAYTEALAGL | RKRQGIDVET | EGRREKAEIA |
| 730 | 740 | 750 | 760 | 770 | 780 |
| KDRDTMIDEL | RALSGNSQFY | PEEVTFVSKE | FVRDLKGASG | EVQQRCCEHF | MLALALCHSV |
| 790 | 800 | 810 | 820 | 830 | 840 |
| LVEANPDNPK | KLDLKAQSPD | EAALVATARD | VGFSFVGKTK | KGLIIEMQGI | QKEFEILNIL |
| 850 | 860 | 870 | 880 | 890 | 900 |
| EFNSSRKRMS | CIVKIPGLNP | GDEPRALLIC | KGADSIIYSR | LSRQSGSNSE | AILEKTALHL |
| 910 | 920 | 930 | 940 | 950 | 960 |
| EQYATEGLRT | LCIAQRELSW | SEYEKWNEKY | DIAAASLANR | EDELEVVADS | IERELILLGG |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| TAIEDRLQDG | VPDCIELLAE | AGIKLWVLTG | DKVETAINIG | FSCNLLNNEM | ELLVIKTTGD |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| DVKEFGSEPS | EIVDALLSKY | LKEYFNLTGS | EEEIFEAKKD | HEFPKGNYAI | VIDGDALKLA |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| LYGEDIRRKF | LLLCKNCRAV | LCCRVSPSQK | AAVVKLVKDS | LDVMTLAIGD | GSNDVAMIQS |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| ADVGIGIAGE | EGRQAVMCSD | YAIGQFRYLA | RLVLVHGRWS | YKRLAEMIPE | FFYKNMIFAL |
| 1210 | 1220 | 1230 | 1240 | 1250 | 1260 |
| ALFWYGIYND | FDGSYLYEYT | YMMFYNLAFT | SLPVIFLGIL | DQDVNDTISL | VVPQLYRVGI |
| 1270 | 1280 | 1290 | 1300 | 1310 | 1320 |
| LRKEWNQRKF | LWYMLDGLYQ | SIICFFFPYL | VYHKNMIVTS | NGLGLDHRYF | VGVYVTTIAV |
| 1330 | 1340 | 1350 | 1360 | 1370 | 1380 |
| ISCNTYVLLH | QYRWDWFSGL | FIALSCLVVF | AWTGIWSSAI | ASREFFKAAA | RIYGAPSFWA |
| 1390 | 1400 | 1410 | 1420 | 1430 | 1440 |
| VFFVAVLFCL | LPRFTYDSFQ | KFFYPTDVEI | VREMWQHGHF | DHYPPGYDPT | DPNRPKVTKA |
| 1450 | 1460 | 1470 | 1480 | 1490 | 1500 |
| GQHGEKIIEG | IALSDNLGGS | NYSRDSVVTE | EIPMTFMHGE | DGSPSGYQKQ | ETWMTSPKET |
| 1510 | 1520 | 1530 | 1540 | 1550 | 1560 |
| QDLLQSPQFQ | QAQTFGRGPS | TNVRSSLDRT | REQMIATNQL | DNRYSVERAR | TSLDLPGVTN |
| 1570 | |||||
| AASLIGTQQN | N |