P32597
PR
Gene name |
STH1 (NPS1, YIL126W) |
Protein name |
Nuclear protein STH1/NPS1 |
Names |
ATP-dependent helicase STH1, Chromatin structure-remodeling complex protein STH1, SNF2 homolog |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YIL126W |
EC number |
3.6.4.12: Acting on ATP; involved in cellular and subcellular movement |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
16 structures for P32597
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 6K15 | EM | 340 A | J | 1-1359 | PDB |
| 6KMB | X-ray | 240 A | A/B/C/D | 1248-1359 | PDB |
| 6KMJ | X-ray | 140 A | A | 1248-1359 | PDB |
| 6KW3 | EM | 713 A | J/W/Y | 1-1359 | PDB |
| 6KW4 | EM | 755 A | J/V/Y | 1-1359 | PDB |
| 6KW5 | EM | 1013 A | J/P/Q | 1-1359 | PDB |
| 6LQZ | NMR | - | B | 1183-1240 | PDB |
| 6MR4 | X-ray | 271 A | A/B/C/D/E/F | 1250-1359 | PDB |
| 6TDA | EM | 1500 A | S | 1-1359 | PDB |
| 6UY1 | X-ray | 221 A | A/B/C/D/E/F/G/H | 1250-1359 | PDB |
| 6V8O | EM | 307 A | R | 1-1359 | PDB |
| 6V92 | EM | 2000 A | R | 1-1359 | PDB |
| 6VZ4 | EM | 390 A | K | 301-1097 | PDB |
| 6VZG | EM | 420 A | K | 301-1097 | PDB |
| 7F3S | X-ray | 140 A | A | 1248-1359 | PDB |
| AF-P32597-F1 | Predicted | AlphaFoldDB |
22 variants for P32597
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s09-118256 | 89 | D>H | No | SGRP | |
| s09-118304 | 105 | D>N | No | SGRP | |
| s09-118307 | 106 | K>E | No | SGRP | |
| s09-118413 | 141 | T>N | No | SGRP | |
| s09-118421 | 144 | D>N | No | SGRP | |
| s09-118634 | 215 | T>A | No | SGRP | |
| s09-118671 | 227 | K>R | No | SGRP | |
| s09-118836 | 282 | T>I | No | SGRP | |
| s09-119631 | 547 | T>I | No | SGRP | |
| s09-119687 | 566 | V>I | No | SGRP | |
| s09-120883 | 964 | S>R | No | SGRP | |
| s09-120903 | 971 | D>G | No | SGRP | |
| s09-120905 | 972 | D>Y | No | SGRP | |
| s09-121004 | 1005 | Q>E | No | SGRP | |
| s09-121125 | 1045 | S>F | No | SGRP | |
| s09-121283 | 1098 | N>H | No | SGRP | |
| s09-121332 | 1114 | A>V | No | SGRP | |
| s09-121348 | 1119 | L>F | No | SGRP | |
| s09-121413 | 1141 | A>V | No | SGRP | |
| s09-121460 | 1157 | I>V | No | SGRP | |
| s09-121652 | 1221 | G>S | No | SGRP | |
| s09-121727 | 1246 | S>P | No | SGRP |
No associated diseases with P32597
1 regional properties for P32597
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Ubiquitin-conjugating enzyme E2 | 74 - 222 | IPR000608 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.6.4.12 | Acting on ATP; involved in cellular and subcellular movement |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| chromatin | The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. |
| chromosome, centromeric region | The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| RSC-type complex | A SWI/SNF-type complex that contains a bromodomain containing-protein, such as yeast Rsc1 or Rsc4 or mammalian PB1/BAF180. The RSC complex is generally recruited to RNA polymerase III promoters and is specifically recruited to RNA polymerase II promoters by transcriptional activators and repressors; it is also involved in non-homologous end joining. |
9 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. |
| ATP-dependent activity, acting on DNA | Catalytic activity that acts to modify DNA, driven by ATP hydrolysis. |
| ATP-dependent chromatin remodeler activity | An activity, driven by ATP hydrolysis, that modulates the contacts between histones and DNA, resulting in a change in chromosome architecture within the nucleosomal array, leading to chromatin remodeling. |
| DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| DNA helicase activity | Unwinding of a DNA helix, driven by ATP hydrolysis. |
| DNA translocase activity | Generation of movement along a single- or double-stranded DNA molecule, driven by ATP hydrolysis. |
| helicase activity | Catalysis of the reaction: ATP + H2O = ADP + phosphate, to drive the unwinding of a DNA or RNA helix. |
| lysine-acetylated histone binding | Binding to a histone in which a lysine residue has been modified by acetylation. |
13 GO annotations of biological process
| Name | Definition |
|---|---|
| base-excision repair | In base excision repair, an altered base is removed by a DNA glycosylase enzyme, followed by excision of the resulting sugar phosphate. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. |
| chromatin remodeling | A dynamic process of chromatin reorganization resulting in changes to chromatin structure. These changes allow DNA metabolic processes such as transcriptional regulation, DNA recombination, DNA repair, and DNA replication. |
| chromatin remodeling at centromere | Dynamic structural changes in centromeric DNA. |
| chromosome segregation | The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles. |
| cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures. |
| double-strand break repair | The repair of double-strand breaks in DNA via homologous and nonhomologous mechanisms to reform a continuous DNA helix. |
| double-strand break repair via nonhomologous end joining | The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends. This term covers instances of separate pathways, called classical (or canonical) and alternative nonhomologous end joining (C-NHEJ and A-NHEJ). These in turn may further branch into sub-pathways, but evidence is still unclear. |
| meiotic cell cycle | Progression through the phases of the meiotic cell cycle, in which canonically a cell replicates to produce four offspring with half the chromosomal content of the progenitor cell via two nuclear divisions. |
| nucleosome disassembly | The controlled breakdown of nucleosomes, the beadlike structural units of eukaryotic chromatin composed of histones and DNA. |
| positive regulation of transcription by RNA polymerase II | Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter. |
| regulation of DNA-templated transcription | Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. |
| transcription elongation by RNA polymerase II promoter | The extension of an RNA molecule after transcription initiation and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II. |
| transfer RNA gene-mediated silencing | The chromatin silencing that results in the inhibition of RNA polymerase II-transcribed genes located in the vicinity of tRNA genes. |
4 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P31380 | FUN30 | ATP-dependent helicase FUN30 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P38144 | ISW1 | ISWI chromatin-remodeling complex ATPase ISW1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| F4K128 | CHR23 | Probable ATP-dependent DNA helicase CHR23 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| F4J9M5 | CHR12 | Probable ATP-dependent DNA helicase CHR12 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MLQEQSELMS | TVMNNTPTTV | AALAAVAAAS | ETNGKLGSEE | QPEITIPKPR | SSAQLEQLLY |
| 70 | 80 | 90 | 100 | 110 | 120 |
| RYRAIQNHPK | ENKLEIKAIE | DTFRNISRDQ | DIYETKLDTL | RKSIDKGFQY | DEDLLNKHLV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| ALQLLEKDTD | VPDYFLDLPD | TKNDNTTAIE | VDYSEKKPIK | ISADFNAKAK | SLGLESKFSN |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ATKTALGDPD | TEIRISARIS | NRINELERLP | ANLGTYSLDD | CLEFITKDDL | SSRMDTFKIK |
| 250 | 260 | 270 | 280 | 290 | 300 |
| ALVELKSLKL | LTKQKSIRQK | LINNVASQAH | HNIPYLRDSP | FTAAAQRSVQ | IRSKVIVPQT |
| 310 | 320 | 330 | 340 | 350 | 360 |
| VRLAEELERQ | QLLEKRKKER | NLHLQKINSI | IDFIKERQSE | QWSRQERCFQ | FGRLGASLHN |
| 370 | 380 | 390 | 400 | 410 | 420 |
| QMEKDEQKRI | ERTAKQRLAA | LKSNDEEAYL | KLLDQTKDTR | ITQLLRQTNS | FLDSLSEAVR |
| 430 | 440 | 450 | 460 | 470 | 480 |
| AQQNEAKILH | GEEVQPITDE | EREKTDYYEV | AHRIKEKIDK | QPSILVGGTL | KEYQLRGLEW |
| 490 | 500 | 510 | 520 | 530 | 540 |
| MVSLYNNHLN | GILADEMGLG | KTIQSISLIT | YLYEVKKDIG | PFLVIVPLST | ITNWTLEFEK |
| 550 | 560 | 570 | 580 | 590 | 600 |
| WAPSLNTIIY | KGTPNQRHSL | QHQIRVGNFD | VLLTTYEYII | KDKSLLSKHD | WAHMIIDEGH |
| 610 | 620 | 630 | 640 | 650 | 660 |
| RMKNAQSKLS | FTISHYYRTR | NRLILTGTPL | QNNLPELWAL | LNFVLPKIFN | SAKTFEDWFN |
| 670 | 680 | 690 | 700 | 710 | 720 |
| TPFANTGTQE | KLELTEEETL | LIIRRLHKVL | RPFLLRRLKK | EVEKDLPDKV | EKVIKCKLSG |
| 730 | 740 | 750 | 760 | 770 | 780 |
| LQQQLYQQML | KHNALFVGAG | TEGATKGGIK | GLNNKIMQLR | KICNHPFVFD | EVEGVVNPSR |
| 790 | 800 | 810 | 820 | 830 | 840 |
| GNSDLLFRVA | GKFELLDRVL | PKFKASGHRV | LMFFQMTQVM | DIMEDFLRMK | DLKYMRLDGS |
| 850 | 860 | 870 | 880 | 890 | 900 |
| TKTEERTEML | NAFNAPDSDY | FCFLLSTRAG | GLGLNLQTAD | TVIIFDTDWN | PHQDLQAQDR |
| 910 | 920 | 930 | 940 | 950 | 960 |
| AHRIGQKNEV | RILRLITTDS | VEEVILERAM | QKLDIDGKVI | QAGKFDNKST | AEEQEAFLRR |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| LIESETNRDD | DDKAELDDDE | LNDTLARSAD | EKILFDKIDK | ERMNQERADA | KAQGLRVPPP |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| RLIQLDELPK | VFREDIEEHF | KKEDSEPLGR | IRQKKRVYYD | DGLTEEQFLE | AVEDDNMSLE |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| DAIKKRREAR | ERRRLRQNGT | KENEIETLEN | TPEASETSLI | ENNSFTAAVD | EETNADKETT |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| ASRSKRRSSR | KKRTISIVTA | EDKENTQEES | TSQENGGAKV | EEEVKSSSVE | IINGSESKKK |
| 1210 | 1220 | 1230 | 1240 | 1250 | 1260 |
| KPKLTVKIKL | NKTTVLENND | GKRAEEKPES | KSPAKKTAAK | KTKTKSKSLG | IFPTVEKLVE |
| 1270 | 1280 | 1290 | 1300 | 1310 | 1320 |
| EMREQLDEVD | SHPRTSIFEK | LPSKRDYPDY | FKVIEKPMAI | DIILKNCKNG | TYKTLEEVRQ |
| 1330 | 1340 | 1350 | |||
| ALQTMFENAR | FYNEEGSWVY | VDADKLNEFT | DEWFKEHSS |