P32491

PR
Gene name
MKK2 (SSP33, YPL140C, LPI6C)
Protein name
MAP kinase kinase MKK2/SSP33
Names
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YPL140C
EC number
2.7.12.2: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-225 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

359-380 (Activation loop from InterPro)

Target domain

214-481 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P32491

Entry ID Method Resolution Chain Position Source
AF-P32491-F1 Predicted AlphaFoldDB

8 variants for P32491

Variant ID(s) Position Change Description Diseaes Association Provenance
s16-288689 115 E>D No SGRP
s16-288583 151 L>F No SGRP
s16-288583 151 L>V No SGRP
s16-288424 204 A>T No SGRP
s16-288400 212 D>N No SGRP
s16-288280 252 P>S No SGRP
s16-288102 311 G>D No SGRP
s16-287520 505 G>E No SGRP

No associated diseases with P32491

3 regional properties for P32491

Type Name Position InterPro Accession
domain Protein kinase domain 214 - 481 IPR000719
active_site Serine/threonine-protein kinase, active site 338 - 350 IPR008271
binding_site Protein kinase, ATP binding site 220 - 243 IPR017441

Functions

Description
EC Number 2.7.12.2 Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cellular bud neck The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding.
cellular bud tip The end of a cellular bud distal to the site of attachment to the mother cell.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mating projection tip The apex of the mating projection in unicellular fungi exposed to mating pheromone; site of polarized growth.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
MAP kinase kinase activity Catalysis of the concomitant phosphorylation of threonine (T) and tyrosine (Y) residues in a Thr-Glu-Tyr (TEY) thiolester sequence in a MAP kinase (MAPK) substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein tyrosine kinase activity Catalysis of the reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

5 GO annotations of biological process

Name Definition
autophagy of peroxisome The process in which peroxisomes are delivered to a type of vacuole and degraded in response to changing nutrient conditions.
cell wall integrity MAPK cascade A MAPK cascade that contributes to cell wall organization or biogenesis.
MAPK cascade An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell.
regulation of fungal-type cell wall organization Any process that modulates the rate, frequency or extent of the formation, arrangement of constituent parts, or disassembly of the fungal-type cell wall.
signal transduction The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

14 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P32490 MKK1 MAP kinase kinase MKK1/SSP32 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q90891 MAP2K2 Dual specificity mitogen-activated protein kinase kinase 2 Gallus gallus (Chicken) PR
Q9XT09 MAP2K1 Dual specificity mitogen-activated protein kinase kinase 1 Pan troglodytes (Chimpanzee) PR
Q24324 Dsor1 Dual specificity mitogen-activated protein kinase kinase dSOR1 Drosophila melanogaster (Fruit fly) PR
Q13163 MAP2K5 Dual specificity mitogen-activated protein kinase kinase 5 Homo sapiens (Human) PR
P36507 MAP2K2 Dual specificity mitogen-activated protein kinase kinase 2 Homo sapiens (Human) EV
Q02750 MAP2K1 Dual specificity mitogen-activated protein kinase kinase 1 Homo sapiens (Human) EV
Q9WVS7 Map2k5 Dual specificity mitogen-activated protein kinase kinase 5 Mus musculus (Mouse) PR
Q63932 Map2k2 Dual specificity mitogen-activated protein kinase kinase 2 Mus musculus (Mouse) PR
P31938 Map2k1 Dual specificity mitogen-activated protein kinase kinase 1 Mus musculus (Mouse) PR
Q62862 Map2k5 Dual specificity mitogen-activated protein kinase kinase 5 Rattus norvegicus (Rat) PR
P36506 Map2k2 Dual specificity mitogen-activated protein kinase kinase 2 Rattus norvegicus (Rat) SS
Q01986 Map2k1 Dual specificity mitogen-activated protein kinase kinase 1 Rattus norvegicus (Rat) PR
Q10664 mek-2 Dual specificity mitogen-activated protein kinase kinase mek-2 Caenorhabditis elegans PR
10 20 30 40 50 60
MASMFRPPES NRSHQKTPKL TLPVNLVQNA KSTNDGQHLN RSPYSSVNES PYSNNSTSAT
70 80 90 100 110 120
STTSSMASNS TLLYNRSSTT TIKNRPVPPP LPPLVLTQKK DGIEYRVAGD SQLSERFSNL
130 140 150 160 170 180
HVDITYKELL SSAPISTKLS NIDTTFIKKD LDTPEGEDSY PSTLLSAYDF SSSGSNSAPL
190 200 210 220 230 240
SANNIISCSN LIQGKDVDQL EEEAWRFGHL KDEITTLGIL GEGAGGSVAK CRLKNGKKVF
250 260 270 280 290 300
ALKTINTMNT DPEYQKQIFR ELQFNKSFKS DYIVQYYGMF TDEQSSSIYI AMEYMGGKSL
310 320 330 340 350 360
EATYKNLLKR GGRISERVIG KIAESVLRGL SYLHERKVIH RDIKPQNILL NEKGEIKLCD
370 380 390 400 410 420
FGVSGEAVNS LAMTFTGTSF YMAPERIQGQ PYSVTCDVWS LGLTLLEVAG GRFPFESDKI
430 440 450 460 470 480
TQNVAPIELL TMILTFSPQL KDEPELDISW SKTFRSFIDY CLKKDARERP SPRQMLKHPW
490 500
IVGQMKKKVN MERFVKKCWE KEKDGI