P32490
Gene name |
MKK1 (SSP32, YOR231W, O5095) |
Protein name |
MAP kinase kinase MKK1/SSP32 |
Names |
|
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YOR231W |
EC number |
2.7.12.2: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues) |
Protein Class |
|
Descriptions
(Annotation based on sequence homology with P36507)
MAP2K2 encodes for Dual specificity mitogen-activated protein kinase kinase 2, belongs to MAP2Ks family, plays an important role in MAP kinase signal transduction pathway. The autoinhibitory state of MAP2K2 is more stable as dimer in the absence of its own substrates or other interacting partners. The several interactions span from the N-terminal to the C-terminal to form dimer. Dimerization of MAP2K2 blocks access to the substrate binding site and the activation loop from macromolecules. MAP2K2 has high sequence similarity with MAP2K1, and the same mechanism of autoinhibition.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
366-387 (Activation loop)
Target domain |
221-488 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
1 structures for P32490
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P32490-F1 | Predicted | AlphaFoldDB |
2 variants for P32490
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s15-773006 | 135 | S>R | No | SGRP | |
| s15-774086 | 495 | N>K | No | SGRP |
No associated diseases with P32490
8 regional properties for P32490
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| repeat | Tetratricopeptide repeat 1 | 83 - 115 | IPR001440 |
| domain | Calcineurin-like phosphoesterase domain, ApaH type | 294 - 488 | IPR004843 |
| domain | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase | 262 - 538 | IPR006186 |
| domain | PPP domain | 227 - 286 | IPR013235 |
| repeat | Tetratricopeptide repeat | 14 - 47 | IPR019734-1 |
| repeat | Tetratricopeptide repeat | 48 - 81 | IPR019734-2 |
| repeat | Tetratricopeptide repeat | 82 - 115 | IPR019734-3 |
| domain | PP5, C-terminal metallophosphatase domain | 234 - 549 | IPR041753 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.12.2 | Dual-specificity kinases (those acting on Ser/Thr and Tyr residues) |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| cellular bud neck | The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding. |
| cellular bud tip | The end of a cellular bud distal to the site of attachment to the mother cell. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| mating projection tip | The apex of the mating projection in unicellular fungi exposed to mating pheromone; site of polarized growth. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| MAP kinase kinase activity | Catalysis of the concomitant phosphorylation of threonine (T) and tyrosine (Y) residues in a Thr-Glu-Tyr (TEY) thiolester sequence in a MAP kinase (MAPK) substrate. |
| protein kinase activity | Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP. |
| protein serine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate. |
| protein serine/threonine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate. |
| protein tyrosine kinase activity | Catalysis of the reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate. |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| autophagy of peroxisome | The process in which peroxisomes are delivered to a type of vacuole and degraded in response to changing nutrient conditions. |
| cell wall integrity MAPK cascade | A MAPK cascade that contributes to cell wall organization or biogenesis. |
| MAPK cascade | An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell. |
| regulation of fungal-type cell wall organization | Any process that modulates the rate, frequency or extent of the formation, arrangement of constituent parts, or disassembly of the fungal-type cell wall. |
| signal transduction | The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. |
14 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P32491 | MKK2 | MAP kinase kinase MKK2/SSP33 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q90891 | MAP2K2 | Dual specificity mitogen-activated protein kinase kinase 2 | Gallus gallus (Chicken) | PR |
| Q9XT09 | MAP2K1 | Dual specificity mitogen-activated protein kinase kinase 1 | Pan troglodytes (Chimpanzee) | PR |
| Q24324 | Dsor1 | Dual specificity mitogen-activated protein kinase kinase dSOR1 | Drosophila melanogaster (Fruit fly) | PR |
| Q13163 | MAP2K5 | Dual specificity mitogen-activated protein kinase kinase 5 | Homo sapiens (Human) | PR |
| P36507 | MAP2K2 | Dual specificity mitogen-activated protein kinase kinase 2 | Homo sapiens (Human) | EV |
| Q02750 | MAP2K1 | Dual specificity mitogen-activated protein kinase kinase 1 | Homo sapiens (Human) | EV |
| Q9WVS7 | Map2k5 | Dual specificity mitogen-activated protein kinase kinase 5 | Mus musculus (Mouse) | PR |
| Q63932 | Map2k2 | Dual specificity mitogen-activated protein kinase kinase 2 | Mus musculus (Mouse) | PR |
| P31938 | Map2k1 | Dual specificity mitogen-activated protein kinase kinase 1 | Mus musculus (Mouse) | PR |
| Q62862 | Map2k5 | Dual specificity mitogen-activated protein kinase kinase 5 | Rattus norvegicus (Rat) | PR |
| P36506 | Map2k2 | Dual specificity mitogen-activated protein kinase kinase 2 | Rattus norvegicus (Rat) | SS |
| Q01986 | Map2k1 | Dual specificity mitogen-activated protein kinase kinase 1 | Rattus norvegicus (Rat) | PR |
| Q10664 | mek-2 | Dual specificity mitogen-activated protein kinase kinase mek-2 | Caenorhabditis elegans | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MASLFRPPES | AKCNPNSPRL | KLPLLRNNQV | DENNIYLTSN | GSSTTAYSSH | TPEPLTSSTS |
| 70 | 80 | 90 | 100 | 110 | 120 |
| TLFSQTRLHP | SDSSMTLNTM | KKRPAPPSLP | SLSINSQSKC | KTLPELVPIA | DVSDGKHDLG |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LKQRVIAENE | LSGNSDLTPS | SMASPFSHTN | TSSPYLRNDL | SNSVGSDFSN | LISAYEQSSS |
| 190 | 200 | 210 | 220 | 230 | 240 |
| PIKSSSQPKS | SSESYIDLNS | VRDVDQLDEN | GWKYANLKDR | IETLGILGEG | AGGSVSKCKL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| KNGSKIFALK | VINTLNTDPE | YQKQIFRELQ | FNRSFQSEYI | VRYYGMFTDD | ENSSIYIAME |
| 310 | 320 | 330 | 340 | 350 | 360 |
| YMGGRSLDAI | YKNLLERGGR | ISEKVLGKIA | EAVLRGLSYL | HEKKVIHRDI | KPQNILLNEN |
| 370 | 380 | 390 | 400 | 410 | 420 |
| GQVKLCDFGV | SGEAVNSLAT | TFTGTSFYMA | PERIQGQPYS | VTSDVWSLGL | TILEVANGKF |
| 430 | 440 | 450 | 460 | 470 | 480 |
| PCSSEKMAAN | IAPFELLMWI | LTFTPELKDE | PESNIIWSPS | FKSFIDYCLK | KDSRERPSPR |
| 490 | 500 | ||||
| QMINHPWIKG | QMKKNVNMEK | FVRKCWKD |