P30625
SS
Gene name |
kin-2 (kin-a, R07E4.6) |
Protein name |
cAMP-dependent protein kinase regulatory subunit |
Names |
|
Species |
Caenorhabditis elegans |
KEGG Pathway |
cel:CELE_R07E4.6 |
EC number |
|
Protein Class |
|
Descriptions
PKA is a major intracellular receptor of cAMP and modulates diverse cellular signalings. The autoinhibitory sequence of the regulatory subunit may interact with the catalytic kinase domain and keeping the catalytic subunit inactive by blocking the active site in the absence of cAMP.
Autoinhibitory domains (AIDs)
Target domain |
122-238 (Cyclic nucleotide-binding domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
References
- Turnham RE et al. (2016) "Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology", Gene, 577, 101-8
- Scott JD (1991) "Cyclic nucleotide-dependent protein kinases", Pharmacology & therapeutics, 50, 123-45
- Diskar M et al. (2007) "Molecular basis for isoform-specific autoregulation of protein kinase A", Cellular signalling, 19, 2024-34
Autoinhibited structure
Activated structure
1 structures for P30625
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P30625-F1 | Predicted | AlphaFoldDB |
No variants for P30625
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P30625 | |||||
No associated diseases with P30625
7 regional properties for P30625
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Cyclic nucleotide-binding domain | 122 - 238 | IPR000595-1 |
| domain | Cyclic nucleotide-binding domain | 240 - 361 | IPR000595-2 |
| domain | cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain | 20 - 57 | IPR003117 |
| conserved_site | Cyclic nucleotide-binding, conserved site | 149 - 165 | IPR018488-1 |
| conserved_site | Cyclic nucleotide-binding, conserved site | 185 - 202 | IPR018488-2 |
| conserved_site | Cyclic nucleotide-binding, conserved site | 267 - 283 | IPR018488-3 |
| conserved_site | Cyclic nucleotide-binding, conserved site | 309 - 326 | IPR018488-4 |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| cAMP-dependent protein kinase complex | An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. Inactive forms of the enzyme have two regulatory chains and two catalytic chains; activation by cAMP produces two active catalytic monomers and a regulatory dimer. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| membrane | A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| cAMP binding | Binding to cAMP, the nucleotide cyclic AMP (adenosine 3',5'-cyclophosphate). |
| cAMP-dependent protein kinase inhibitor activity | Binds to and stops, prevents or reduces the activity of a cAMP-dependent protein kinase. |
| cAMP-dependent protein kinase regulator activity | Modulation of the activity of the enzyme cAMP-dependent protein kinase. |
| protein kinase A catalytic subunit binding | Binding to one or both of the catalytic subunits of protein kinase A. |
6 GO annotations of biological process
| Name | Definition |
|---|---|
| medium-term memory | The memory process that deals with the storage, retrieval and modification of information received at a time ago that is intermediate between that of short and long term memory (30min - 7hrs in Drosophila melanogaster). |
| negative regulation of oocyte maturation | Any process that stops, prevents or reduces the frequency, rate or extent of oocyte maturation. |
| negative regulation of protein kinase C signaling | Any process that decreases the frequency, rate, or extent of a series of reactions, mediated by the intracellular serine/threonine kinase protein kinase C, which occurs as a result of a single trigger reaction or compound. |
| positive regulation of gene expression | Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA). |
| regulation of establishment of cell polarity | Any process that modulates the frequency, rate or extent of establishment of cell polarity. |
| regulation of protein phosphorylation | Any process that modulates the frequency, rate or extent of addition of phosphate groups into an amino acid in a protein. |
22 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P00515 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Bos taurus (Bovine) | EV |
| P31322 | PRKAR2B | cAMP-dependent protein kinase type II-beta regulatory subunit | Bos taurus (Bovine) | EV |
| P00514 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Bos taurus (Bovine) | EV |
| Q5ZM91 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Gallus gallus (Chicken) | SS |
| P81900 | Pka-R2 | cAMP-dependent protein kinase type II regulatory subunit | Drosophila melanogaster (Fruit fly) | PR |
| P16905 | Pka-R1 | cAMP-dependent protein kinase type I regulatory subunit | Drosophila melanogaster (Fruit fly) | PR |
| P13861 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Homo sapiens (Human) | PR |
| P31323 | PRKAR2B | cAMP-dependent protein kinase type II-beta regulatory subunit | Homo sapiens (Human) | PR |
| Q96M20 | CNBD2 | Cyclic nucleotide-binding domain-containing protein 2 | Homo sapiens (Human) | PR |
| P31321 | PRKAR1B | cAMP-dependent protein kinase type I-beta regulatory subunit | Homo sapiens (Human) | EV |
| P10644 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Homo sapiens (Human) | EV |
| P12367 | Prkar2a | cAMP-dependent protein kinase type II-alpha regulatory subunit | Mus musculus (Mouse) | PR |
| P31324 | Prkar2b | cAMP-dependent protein kinase type II-beta regulatory subunit | Mus musculus (Mouse) | PR |
| Q9D5U8 | Cnbd2 | Cyclic nucleotide-binding domain-containing protein 2 | Mus musculus (Mouse) | PR |
| Q9DBC7 | Prkar1a | cAMP-dependent protein kinase type I-alpha regulatory subunit [Cleaved into: cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed] | Mus musculus (Mouse) | SS |
| P12849 | Prkar1b | cAMP-dependent protein kinase type I-beta regulatory subunit | Mus musculus (Mouse) | PR |
| P05207 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Sus scrofa (Pig) | SS |
| P07802 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Sus scrofa (Pig) | SS |
| P12369 | Prkar2b | cAMP-dependent protein kinase type II-beta regulatory subunit | Rattus norvegicus (Rat) | PR |
| P12368 | Prkar2a | cAMP-dependent protein kinase type II-alpha regulatory subunit | Rattus norvegicus (Rat) | PR |
| P09456 | Prkar1a | cAMP-dependent protein kinase type I-alpha regulatory subunit | Rattus norvegicus (Rat) | SS |
| P81377 | Prkar1b | cAMP-dependent protein kinase type I-beta regulatory subunit | Rattus norvegicus (Rat) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSGGNEEDQL | AQCQAYVQRH | NIQQLVKEAI | VVLCIHKPDN | PVLFLKDHFE | KLNEQRAQEG |
| 70 | 80 | 90 | 100 | 110 | 120 |
| GNPDAADDDD | IIVEPPKRSG | GRRTGISAEP | IKEDDTEYKK | VVIPKDDATR | RSLESAMRKN |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LLFAHLEEDE | QKTMYDAMFP | VEKSAGETII | EQGEEGDNFY | VIDKGTVDVY | VNHEYVLTIN |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EGGSFGELAL | IYGTPRAATV | IAKTDVKLWA | IDRLTYRRIL | MGSVTKKRKM | YDEFLSKVQI |
| 250 | 260 | 270 | 280 | 290 | 300 |
| LADLDQWERA | NVADALERCD | FEPGTHVVEQ | GQPGDEFFII | LEGEANVLQK | RSDDAPFDVV |
| 310 | 320 | 330 | 340 | 350 | 360 |
| GHLGMSDYFG | EIALLLDRPR | AATVVAKTHL | KCIKLDRNRF | ERVMGPVREI | LKRDVSNYNS |
| YVKLMT |