P29397

EV
Gene name
nusG (TM_0453)
Protein name
Transcription termination/antitermination protein NusG
Names
Species
Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
KEGG Pathway
tma:TM0453
EC number
Protein Class
RHO-INTERACTING TRANSCRIPTION TERMINATION FACTOR NUSG (PTHR30265)

Descriptions

NusG from Thermotoga maritima exhibits a unique autoinhibition mechanism where its N-terminal domain (NTD) and C-terminal domain (CTD) interact tightly, masking the binding sites for interaction partners such as Rho, NusE, and RNA polymerase (RNAP). This interaction stabilizes the CTD but inhibits NusG's function in transcription regulation by preventing its interaction with other transcription factors and RNAP.

Autoinhibitory domains (AIDs)

296-351 (C-terminal domain) EV

Target domain

1-284 (N-terminal domain for interaction with RNAP)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment, Structural analysis

1-284 (N-terminal domain for interaction with RNAP) EV

Target domain

296-351 (C-terminal domain)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

4 structures for P29397

Entry ID Method Resolution Chain Position Source
2LQ8 NMR - PDB
2XHA X-ray 191 A A/B 41-233 PDB
2XHC X-ray 245 A A 1-352 PDB
AF-P29397-F1 Predicted AlphaFoldDB

No variants for P29397

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P29397

No associated diseases with P29397

2 regional properties for P29397

Type Name Position InterPro Accession
domain Ets domain 162 - 248 IPR000418
domain Pointed domain 33 - 119 IPR003118

Functions

Description
EC Number
Subcellular Localization
PANTHER Family PTHR30265 RHO-INTERACTING TRANSCRIPTION TERMINATION FACTOR NUSG
PANTHER Subfamily PTHR30265:SF2 TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSG
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

No GO annotations of molecular function

Name Definition
No GO annotations for molecular function

4 GO annotations of biological process

Name Definition
DNA-templated transcription termination The completion of transcription
regulation of DNA-templated transcription elongation Any process that modulates the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides catalyzed by a DNA-dependent RNA polymerase.
transcription antitermination A positive regulation of gene expression mechanism that allows RNA polymerase to continue transcription beyond a termination site, thus allowing expression of downstream genes under specific conditions.
transcription elongation-coupled chromatin remodeling A chromatin remodeling process that reestablishes the chromatin structure following the passage of RNA polymerase II during transcription elongation, thus preventing cryptic transcription initiation.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MKKKWYIVLT MSGYEEKVKE NIEKKVEATG IKNLVGRIVI PEEVVLDATS PSERLILSPK
70 80 90 100 110 120
AKLHVNNGKD VNKGDLIAEE PPIYARRSGV IVDVKNVRKI VVETIDRKYT KTYYIPESAG
130 140 150 160 170 180
IEPGLRVGTK VKQGLPLSKN EEYICELDGK IVEIERMKKV VVQTPDGEQD VYYIPLDVFD
190 200 210 220 230 240
RDRIKKGKEV KQGEMLAEAR KFFAKVSGRV EVVDYSTRKE IRIYKTKRRK LFPGYVFVEM
250 260 270 280 290 300
IMNDEAYNFV RSVPYVMGFV SSGGQPVPVK DREMRPILRL AGLEEYEEKK KPVKVELGFK
310 320 330 340 350
VGDMVKIISG PFEDFAGVIK EIDPERQELK VNVTIFGRET PVVLHVSEVE KIE