P29319
SS
Gene name |
Epha3 (Etk1, Mek4, Tyro4) |
Protein name |
Ephrin type-A receptor 3 |
Names |
EC 2.7.10.1 , EPH-like kinase 4 , EK4 , mEK4 , Tyrosine-protein kinase TYRO4 , Tyrosine-protein kinase receptor ETK1 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
|
EC number |
2.7.10.1: Protein-tyrosine kinases |
Protein Class |
|
Descriptions
Ephrin type-B receptor 2 is a membrane-associated protein that mediates axon guidance, cell migration and morphogenesis. The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. The structure, supported by mutagenesis data, reveals that the juxtamembrane segment adopts a helical conformation that distorts the small lobe of the kinase domain, and blocks the activation segment from attaining an activated conformation. Phosphorylation of the conserved juxtamembrane tyrosines would relieve this autoinhibition by disturbing the association of the juxtamembrane segment with the kinase domain, while liberating phosphotyrosine sites for binding SH2 domains of target proteins.
Autoinhibitory domains (AIDs)
Target domain |
621-882 (Protein kinase domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
763-789 (Activation loop from InterPro)
Target domain |
621-882 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
Autoinhibited structure
Activated structure
1 structures for P29319
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P29319-F1 | Predicted | AlphaFoldDB |
No variants for P29319
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P29319 | |||||
No associated diseases with P29319
14 regional properties for P29319
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 621 - 882 | IPR000719 |
| domain | Ephrin receptor ligand binding domain | 29 - 206 | IPR001090 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 622 - 878 | IPR001245 |
| conserved_site | Tyrosine-protein kinase, receptor class V, conserved site | 182 - 202 | IPR001426-1 |
| conserved_site | Tyrosine-protein kinase, receptor class V, conserved site | 243 - 263 | IPR001426-2 |
| domain | Sterile alpha motif domain | 908 - 975 | IPR001660 |
| domain | Fibronectin type III | 324 - 434 | IPR003961-1 |
| domain | Fibronectin type III | 435 - 530 | IPR003961-2 |
| active_site | Tyrosine-protein kinase, active site | 742 - 754 | IPR008266 |
| domain | Tyrosine-protein kinase ephrin type A/B receptor-like | 269 - 302 | IPR011641 |
| binding_site | Protein kinase, ATP binding site | 627 - 653 | IPR017441 |
| domain | Tyrosine-protein kinase, catalytic domain | 621 - 878 | IPR020635 |
| domain | Ephrin receptor, transmembrane domain | 543 - 618 | IPR027936 |
| domain | Ephrin type-A receptor 3, ligand binding domain | 29 - 200 | IPR034266 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.10.1 | Protein-tyrosine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| dendrite | A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body. |
| early endosome | A membrane-bounded organelle that receives incoming material from primary endocytic vesicles that have been generated by clathrin-dependent and clathrin-independent endocytosis; vesicles fuse with the early endosome to deliver cargo for sorting into recycling or degradation pathways. |
| extracellular region | The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite. |
| GABA-ergic synapse | A synapse that uses GABA as a neurotransmitter. These synapses are typically inhibitory. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| presynapse | The part of a synapse that is part of the presynaptic cell. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ephrin receptor activity | Combining with an ephrin receptor ligand to initiate a change in cell activity. |
| GPI-linked ephrin receptor activity | Combining with a GPI-anchored ephrin to initiate a change in cell activity. |
| growth factor binding | Binding to a growth factor, proteins or polypeptides that stimulate a cell or organism to grow or proliferate. |
| transmembrane-ephrin receptor activity | Combining with a transmembrane ephrin to initiate a change in cell activity. |
16 GO annotations of biological process
| Name | Definition |
|---|---|
| axon guidance | The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues. |
| cell migration | The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. |
| cellular response to follicle-stimulating hormone stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a follicle-stimulating hormone stimulus. |
| endocardial cushion development | The progression of a cardiac cushion over time, from its initial formation to the mature structure. The endocardial cushion is a specialized region of mesenchymal cells that will give rise to the heart septa and valves. |
| ephrin receptor signaling pathway | The series of molecular signals initiated by ephrin binding to its receptor, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| fasciculation of motor neuron axon | The collection of motor neuron axons into a bundle of rods, known as a fascicle. |
| fasciculation of sensory neuron axon | The collection of sensory neuron axons into a bundle of rods, known as a fascicle. |
| negative regulation of neuron projection development | Any process that decreases the rate, frequency or extent of neuron projection development. Neuron projection development is the process whose specific outcome is the progression of a neuron projection over time, from its formation to the mature structure. A neuron projection is any process extending from a neural cell, such as axons or dendrites (collectively called neurites). |
| phosphorylation | The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. |
| regulation of actin cytoskeleton organization | Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| regulation of cell migration | Any process that modulates the frequency, rate or extent of cell migration. |
| regulation of epithelial to mesenchymal transition | Any process that modulates the rate, frequency, or extent of epithelial to mesenchymal transition. Epithelial to mesenchymal transition where an epithelial cell loses apical/basolateral polarity, severs intercellular adhesive junctions, degrades basement membrane components and becomes a migratory mesenchymal cell. |
| regulation of focal adhesion assembly | Any process that modulates the frequency, rate or extent of focal adhesion formation, the establishment and maturation of focal adhesions. |
| regulation of GTPase activity | Any process that modulates the rate of GTP hydrolysis by a GTPase. |
| regulation of microtubule cytoskeleton organization | Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins. |
| synaptic membrane adhesion | The attachment of presynaptic membrane to postsynaptic membrane via adhesion molecules that are at least partially embedded in the plasma membrane. |
49 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P28693 | EPHB2 | Ephrin type-B receptor 2 | Gallus gallus (Chicken) | PR |
| Q07496 | EPHA4 | Ephrin type-A receptor 4 | Gallus gallus (Chicken) | SS |
| Q07494 | EPHB1 | Ephrin type-B receptor 1 | Gallus gallus (Chicken) | SS |
| Q07497 | EPHB5 | Ephrin type-B receptor 5 | Gallus gallus (Chicken) | PR |
| O42422 | EPHA7 | Ephrin type-A receptor 7 | Gallus gallus (Chicken) | SS |
| P54755 | EPHA5 | Ephrin type-A receptor 5 | Gallus gallus (Chicken) | SS |
| Q07498 | EPHB3 | Ephrin type-B receptor 3 | Gallus gallus (Chicken) | SS |
| P29318 | EPHA3 | Ephrin type-A receptor 3 | Gallus gallus (Chicken) | SS |
| P0C0K6 | EPHB6 | Ephrin type-B receptor 6 | Pan troglodytes (Chimpanzee) | SS |
| P29322 | EPHA8 | Ephrin type-A receptor 8 | Homo sapiens (Human) | SS |
| P21709 | EPHA1 | Ephrin type-A receptor 1 | Homo sapiens (Human) | SS |
| P54764 | EPHA4 | Ephrin type-A receptor 4 | Homo sapiens (Human) | SS |
| P54753 | EPHB3 | Ephrin type-B receptor 3 | Homo sapiens (Human) | SS |
| P54760 | EPHB4 | Ephrin type-B receptor 4 | Homo sapiens (Human) | SS |
| P29317 | EPHA2 | Ephrin type-A receptor 2 | Homo sapiens (Human) | SS |
| Q15375 | EPHA7 | Ephrin type-A receptor 7 | Homo sapiens (Human) | SS |
| Q5JZY3 | EPHA10 | Ephrin type-A receptor 10 | Homo sapiens (Human) | SS |
| P29323 | EPHB2 | Ephrin type-B receptor 2 | Homo sapiens (Human) | EV |
| Q9UF33 | EPHA6 | Ephrin type-A receptor 6 | Homo sapiens (Human) | SS |
| P54762 | EPHB1 | Ephrin type-B receptor 1 | Homo sapiens (Human) | SS |
| P54756 | EPHA5 | Ephrin type-A receptor 5 | Homo sapiens (Human) | SS |
| O15197 | EPHB6 | Ephrin type-B receptor 6 | Homo sapiens (Human) | SS |
| P29320 | EPHA3 | Ephrin type-A receptor 3 | Homo sapiens (Human) | PR |
| Q03145 | Epha2 | Ephrin type-A receptor 2 | Mus musculus (Mouse) | PR |
| Q60629 | Epha5 | Ephrin type-A receptor 5 | Mus musculus (Mouse) | SS |
| Q60750 | Epha1 | Ephrin type-A receptor 1 | Mus musculus (Mouse) | SS |
| P54761 | Ephb4 | Ephrin type-B receptor 4 | Mus musculus (Mouse) | PR |
| O08644 | Ephb6 | Ephrin type-B receptor 6 | Mus musculus (Mouse) | PR |
| O09127 | Epha8 | Ephrin type-A receptor 8 | Mus musculus (Mouse) | SS |
| P54754 | Ephb3 | Ephrin type-B receptor 3 | Mus musculus (Mouse) | SS |
| P54763 | Ephb2 | Ephrin type-B receptor 2 | Mus musculus (Mouse) | SS |
| Q03137 | Epha4 | Ephrin type-A receptor 4 | Mus musculus (Mouse) | SS |
| Q61772 | Epha7 | Ephrin type-A receptor 7 | Mus musculus (Mouse) | SS |
| Q8BYG9 | Epha10 | Ephrin type-A receptor 10 | Mus musculus (Mouse) | SS |
| Q8CBF3 | Ephb1 | Ephrin type-B receptor 1 | Mus musculus (Mouse) | SS |
| Q62413 | Epha6 | Ephrin type-A receptor 6 | Mus musculus (Mouse) | SS |
| P54757 | Epha5 | Ephrin type-A receptor 5 | Rattus norvegicus (Rat) | SS |
| P54759 | Epha7 | Ephrin type-A receptor 7 | Rattus norvegicus (Rat) | SS |
| P09759 | Ephb1 | Ephrin type-B receptor 1 | Rattus norvegicus (Rat) | SS |
| P0C0K7 | Ephb6 | Ephrin type-B receptor 6 | Rattus norvegicus (Rat) | SS |
| O08680 | Epha3 | Ephrin type-A receptor 3 | Rattus norvegicus (Rat) | SS |
| O61460 | vab-1 | Ephrin receptor 1 | Caenorhabditis elegans | SS |
| Q9LMN7 | WAK5 | Wall-associated receptor kinase 5 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LSV3 | WAKL16 | Putative wall-associated receptor kinase-like 16 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9M342 | WAKL15 | Wall-associated receptor kinase-like 15 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8VZJ9 | CRCK2 | Calmodulin-binding receptor-like cytoplasmic kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| O13147 | ephb3 | Ephrin type-B receptor 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| O73878 | ephb4b | Ephrin type-B receptor 4b | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| O13146 | epha3 | Ephrin type-A receptor 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MDCHLSILVL | LGCCVLSCSG | ELSPQPSNEV | NLLDSKTIQG | ELGWISYPSH | GWEEISGVDE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| HYTPIRTYQV | CNVMDHSQNN | WLRTNWVPRN | SAQKIYVELK | FTLRDCNSIP | LVLGTCKETF |
| 130 | 140 | 150 | 160 | 170 | 180 |
| NLYYMESDDH | GVKFREHQFT | KIDTIAADES | FTQMDLGDRI | LKLNTEIREV | GPVNKKGFYL |
| 190 | 200 | 210 | 220 | 230 | 240 |
| AFQDVGACVA | LVSVRVYFKK | CPFTVKNLAM | FPDTVPMDSQ | SLVEVRGSCV | NNSKEEDPPR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| MYCSTEGEWL | VPIGKCTCNA | GYEERGFICQ | ACRPGFYKAS | DGAAKCAKCP | PHSSTQEDGS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| MNCRCENNYF | RAEKDPPSMA | CARPPSAPRN | VISNINETSV | ILDWSWPLDT | GGRKDITFNI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| ICKKCGWNVR | QCEPCSPNVR | FLPRQLGLTN | TTVTVTDLLA | HTNYTFEIDA | VNGVSELSSP |
| 430 | 440 | 450 | 460 | 470 | 480 |
| PRQYAAVSIT | TNQAAPSPVM | TIKKDRTSRN | SISLSWQEPE | HPNGIILDYE | VKYYQKQEQE |
| 490 | 500 | 510 | 520 | 530 | 540 |
| TSYTILRARG | TNVTISSLKP | DTTYVFQIRA | RTAAGYGTNS | RKFEFETSPD | SFSISGENSH |
| 550 | 560 | 570 | 580 | 590 | 600 |
| VVMIAISAAV | AIIVLTVVTY | VLVGRFCGYH | KSKHSAEEKR | LHFGNGHLKL | PGLRTYVDPH |
| 610 | 620 | 630 | 640 | 650 | 660 |
| TYEDPTQAVH | EFAKELDATN | ISIDKVVGAG | EFGEVCSGRL | KLPSKKEISV | AIKTLKVGYT |
| 670 | 680 | 690 | 700 | 710 | 720 |
| EKQRRDFLGE | ASIMGQFDHP | NIIRLEGVVT | KSKPEMIVTE | YMENGSLDSF | LRKHDAQFTV |
| 730 | 740 | 750 | 760 | 770 | 780 |
| IQLVGMLRGI | ASGMKYLSDM | GYVHRDLAAR | NILINSNLVC | KVSDFGLSRV | LEDDPEAAYT |
| 790 | 800 | 810 | 820 | 830 | 840 |
| TRGGKIPIRW | TSPEAMSYRK | FTSASDVWSY | GIVLWEVMSY | GERPYSQMSN | QDVIKAVDER |
| 850 | 860 | 870 | 880 | 890 | 900 |
| YRLPPPMDCP | AALYQLMLDC | WQKDRNNRPK | FEQIVSILDK | LIRNPGSLKI | ITSAAARPSN |
| 910 | 920 | 930 | 940 | 950 | 960 |
| LLLDQSNVDI | ATFHTTGDWL | NGMRTAHCKE | IFTGVEYSSC | DTIAKISTDD | MKKVGVTVVG |
| 970 | 980 | ||||
| PQKKIISTIK | ALETQSKNGP | VPV |