P29294

EV
Gene name
MYLK
Protein name
Myosin light chain kinase, smooth muscle
Names
MLCK, smMLCK, Telokin
Species
Oryctolagus cuniculus (Rabbit)
KEGG Pathway
ocu:100009143
EC number
Protein Class
IMMUNOGLOBULIN (PTHR47633)

Descriptions

Myosin light chain kinases (MLCK) are members of the family of Ca2+-calmodulin-dependent protein kinases. The autoinhibitory pseudosubstrate sequence was identified by sequence similarity with the chicken smooth muscle MLCK (P11799-2). The mutational changes of acidic residues in the catalytic core of the kinase domain, which may interact with the basic residues in the pseudosubstrate sequence, increased the catalytic activity of the kinase domain.

Autoinhibitory domains (AIDs)

964-992 (Autoinhibitory pseudosubstrate) EV

Target domain

696-951 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment, Deletion assay

Accessory elements

836-858 (Activation loop from InterPro)

Target domain

696-951 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P29294

Entry ID Method Resolution Chain Position Source
AF-P29294-F1 Predicted AlphaFoldDB

No variants for P29294

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P29294

No associated diseases with P29294

11 regional properties for P29294

Type Name Position InterPro Accession
domain Protein kinase domain 696 - 951 IPR000719
domain Immunoglobulin subtype 335 - 419 IPR003599-1
domain Immunoglobulin subtype 475 - 559 IPR003599-2
domain Immunoglobulin subtype 1047 - 1132 IPR003599-3
domain Fibronectin type III 562 - 657 IPR003961
domain Immunoglobulin-like domain 329 - 417 IPR007110-1
domain Immunoglobulin-like domain 469 - 557 IPR007110-2
domain Immunoglobulin-like domain 1041 - 1130 IPR007110-3
active_site Serine/threonine-protein kinase, active site 813 - 825 IPR008271
domain Myosin Light Chain Kinase 1, Kinase domain 693 - 951 IPR015725
binding_site Protein kinase, ATP binding site 702 - 725 IPR017441

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Cell projection, lamellipodium
  • Cleavage furrow
  • Cytoplasm, cytoskeleton, stress fiber
  • Localized to stress fibers during interphase and to the cleavage furrow during mitosis
PANTHER Family PTHR47633 IMMUNOGLOBULIN
PANTHER Subfamily PTHR47633:SF1 MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE
PANTHER Protein Class
PANTHER Pathway Category Inflammation mediated by chemokine and cytokine signaling pathway
MLCK
Cytoskeletal regulation by Rho GTPase
MLCK

4 GO annotations of cellular component

Name Definition
cleavage furrow The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoskeleton A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
lamellipodium A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments.

5 GO annotations of molecular function

Name Definition
actin binding Binding to monomeric or multimeric forms of actin, including actin filaments.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
metal ion binding Binding to a metal ion.
myosin light chain kinase activity Catalysis of the reaction: ATP + myosin-light-chain = ADP + myosin-light-chain phosphate.

No GO annotations of biological process

Name Definition
No GO annotations for biological process

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MDFRANLQRQ VKPKTVSEEE RKVHSPQQVD FRSVLAKKGT PKTPVPEKAP PPKPATPDFR
70 80 90 100 110 120
SVLGSKKKLP AENGSSNAEA LNVKATESPK LVGNAPLSGS LKPVANAKPA ETLKPVANTK
130 140 150 160 170 180
PAETLKPVAN AETLKPMGNA KPAESSKPVG NTKPAETLKP VGNTKPAETL KPVGNIKPAE
190 200 210 220 230 240
TLKPVGNIKP AETLKPVGNT KPTETLKPVA NAKSAETLKP IANTKPAETL KPVGNAKPAE
250 260 270 280 290 300
TLKPVGNAKP AETLKPVGNA KPAETLKPVG NAKPAETLKA VANAKPAETP KPAGKEELKK
310 320 330 340 350 360
EVQNDVNCKR EKAGAADNEK PPASPGTAPT FKEKLQDVRV AEGEKLLLQC QVSSEPPATI
370 380 390 400 410 420
TWTLNGKTLK TTKFVILSQE GSLCSVSIEK ALPEDRGLYK CVAKNAAPEA ECSCHVTVHD
430 440 450 460 470 480
APASENAKAP EMKSRRPKSS LPPVLGTESD ATVKKKPAPK TPPKAATPPQ IPQFPEDQKV
490 500 510 520 530 540
RAGERVELFG KVAGTQPITC TWMKFRKQIQ DSEHIKVENS EAGSKLTILA ARQEHCGCYT
550 560 570 580 590 600
LLVENKLGSR QAQVNLTVVD KPDPPAGTPC ASDIRSSSLT LSWYGSSYDG GSAVQSYSVE
610 620 630 640 650 660
IWDSVDKMWT ELATCRSTSF NVRDLLPDRE YKFRVRAINV YGTSEPSQES ELTTVGEKPE
670 680 690 700 710 720
EPKDEVEEVS DDDEKEPEVD YRTVTVNTEQ KVSDFYDIEE RLGSGKFGQV FRLVEKKTGK
730 740 750 760 770 780
IWAGKFFKAY SAKEKENIPA EIGIMNCLHH PKLVQCVDAF EEKANIVMVL EIVSGGELFE
790 800 810 820 830 840
RIIDEDFELT ERECIKYMRQ ISEGVEYIHK QGIVHLDLKP ENIMCVNKTG TRIKLIDFGL
850 860 870 880 890 900
ARRLENAGSL KVLFGTPEFV APEVINYEPI SYATDMWSIG VICYILVSGL SPFMGDNDNE
910 920 930 940 950 960
TLANVTSATW DFDDEAFDEI SDDAKDFISN LLKKDMKNRL DCTQCLQHPW LMKDTKNMEA
970 980 990 1000 1010 1020
KKLSKDRMKK YMARRKWQKT GNAVRAIGRL SSMAMISGLS GRKSSTGSPT SPLTAERLET
1030 1040 1050 1060 1070 1080
EEDVSQAFLE AVAEEKPHVK PYFSKTIRDL EVVEGSAARF DCKIEGYPDP EVVWFKDDQS
1090 1100 1110 1120 1130 1140
IRESRHFQID YDEDGNCSLI ISDVCGDDDA KYTCKAVNSL GEATCTAELI VETMEEGEGE
GEEEEEE