P28829

EV
Gene name
byr2
Protein name
Protein kinase byr2
Names
MAPK kinase kinase, MAPKKK, Protein kinase ste8
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
KEGG Pathway
spo:SPBC1D7.05
EC number
2.7.11.25: Protein-serine/threonine kinases
Protein Class
MAP KINASE KINASE KINASE SSK2-RELATED-RELATED (PTHR48016)

Descriptions

Byr2 protein kinase is involved in pheromone-induced sexual differentiation in the fission yeast Schizosaccharomyces pombe. Byr2 has a distinctive N-terminal kinase regulatory domain and a characteristic C-terminal kinase catalytic domain in the two-hybrid system. The Byr2 regulatory domain binds Ste4 and Ras1 and also binds to the catalytic domain. The intramolecular binding between the regulatory and catalytic domains regulates the catalytic activity of Byr2, and it is suggested that this interaction is autoinhibitory.

Autoinhibitory domains (AIDs)

151-260 (regulatory domain) EV

Target domain

394-658 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Split protein assay, Deletion assay, Mutagenesis experiment

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

3 structures for P28829

Entry ID Method Resolution Chain Position Source
1I35 NMR - A 71-165 PDB
1K8R X-ray 300 A B 71-180 PDB
AF-P28829-F1 Predicted AlphaFoldDB

No variants for P28829

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P28829

No associated diseases with P28829

5 regional properties for P28829

Type Name Position InterPro Accession
domain Protein kinase domain 394 - 658 IPR000719
domain Sterile alpha motif domain 1 - 67 IPR001660
active_site Serine/threonine-protein kinase, active site 518 - 530 IPR008271
binding_site Protein kinase, ATP binding site 400 - 423 IPR017441
domain Ras-binding domain of Byr2 72 - 166 IPR029458

Functions

Description
EC Number 2.7.11.25 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
PANTHER Family PTHR48016 MAP KINASE KINASE KINASE SSK2-RELATED-RELATED
PANTHER Subfamily PTHR48016:SF29 MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 1A
PANTHER Protein Class non-receptor serine/threonine protein kinase
protein modifying enzyme
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cell division site The eventual plane of cell division (also known as cell cleavage or cytokinesis) in a dividing cell. In Eukaryotes, the cleavage apparatus, composed of septin structures and the actomyosin contractile ring, forms along this plane, and the mitotic, or meiotic, spindle is aligned perpendicular to the division plane. In bacteria, the cell division site is generally located at mid-cell and is the site at which the cytoskeletal structure, the Z-ring, assembles.
cell tip The region at the end of the longest axis of a cylindrical or elongated cell.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
division septum A cell septum which forms as part of the division site and functions in the compartmentalization of a cell into two daughter cells at division. A division septum spans a cell and does not allow exchange of organelles or cytoplasm between compartments.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
MAP kinase kinase kinase activity Catalysis of the phosphorylation and activation of a MAP kinase kinase; each MAP kinase kinase can be phosphorylated by any of several MAP kinase kinase kinases.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

2 GO annotations of biological process

Name Definition
induction of conjugation with cellular fusion The process in which a cell initiates conjugation with cellular fusion. Conjugation with cellular fusion is the process that results in the union of cellular and genetic information from compatible mating types.
pheromone response MAPK cascade A MAPK cascade that is part of a pheromone response ending in conjugation with cellular fusion.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MEYYTSKEVA EWLKSIGLEK YIEQFSQNNI EGRHLNHLTL PLLKDLGIEN TAKGKQFLKQ
70 80 90 100 110 120
RDYLREFPRP CILRFIACNG QTRAVQSRGD YQKTLAIALK KFSLEDASKF IVCVSQSSRI
130 140 150 160 170 180
KLITEEEFKQ ICFNSSSPER DRLIIVPKEK PCPSFEDLRR SWEIELAQPA ALSSQSSLSP
190 200 210 220 230 240
KLSSVLPTST QKRSVRSNNA KPFESYQRPP SELINSRISD FFPDHQPKLL EKTISNSLRR
250 260 270 280 290 300
NLSIRTSQGH NLGNFGQEIL PRSSRRARPS ELVCPLSSLR ISVAEDVNRL PRIDRGFDPP
310 320 330 340 350 360
LTVSSTQRIS RPPSLQKSIT MVGVEPLYQS NGNEKSSKYN VFSESAHGNH QVLSFSPGSS
370 380 390 400 410 420
PSFIEQPSPI SPTSTTSEDT NTLEEDTDDQ SIKWIRGALI GSGSFGQVYL GMNASSGELM
430 440 450 460 470 480
AVKQVILDSV SESKDRHAKL LDALAGEIAL LQELSHEHIV QYLGSNLNSD HLNIFLEYVP
490 500 510 520 530 540
GGSVAGLLTM YGSFEETLVK NFIKQTLKGL EYLHSRGIVH RDIKGANILV DNKGKIKISD
550 560 570 580 590 600
FGISKKLELN STSTKTGGAR PSFQGSSFWM APEVVKQTMH TEKTDIWSLG CLVIEMLTSK
610 620 630 640 650
HPYPNCDQMQ AIFRIGENIL PEFPSNISSS AIDFLEKTFA IDCNLRPTAS ELLSHPFVS