AiPD: Autoinhibited Protein Database

P28482

Gene name	MAPK1 (ERK2, PRKM1, PRKM2)
Protein name	Mitogen-activated protein kinase 1
Names	MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2
Species	Homo sapiens (Human)
KEGG Pathway	hsa:5594
EC number	2.7.11.24: Protein-serine/threonine kinases
Protein Class	MITOGEN-ACTIVATED PROTEIN KINASE (PTHR24055)

Descriptions

MAPK1 encodes Mitogen-activated protein kinase 1, and is involved in diverse cellular responses by phosphorylating cytoplasmic and nuclear proteins. Unlike other MAP kinases, MAPK1 activates via dual phosphorylation of Thr-186 and Tyr-193 within the activation lip, and mutations at Q105 alone were sufficient for autoactivation. Additionally, in the inactive form, L76, Q10 and I87 form a structural unit that restrains intramolecular autophosphorylation by preventing interactions between activation lip residues and the catalytic base (D149) needed for phosphoryl transfer. Mutations at L76, I87 and additionally S153 increased basal specific activity via autophosphorylation of Thr-186 and Tyr-193.

Autoinhibitory domains (AIDs)

Target domain	25-313 (Protein kinase domain)
Relief mechanism	PTM
Assay	Mutagenesis experiment, Structural analysis

AID

73-151 (Autoinhibitory region)

Target domain

25-313 (Protein kinase domain)

Relief mechanism

PTM (Autophosphorylation at Thr-186 and Tyr-193)

Assay

Mutagenesis experiment, Structural analysis

Accessory elements

166-192 (Activation loop from InterPro)

Target domain	25-313 (Protein kinase domain)
Relief mechanism
Assay

References

Emrick MA et al. (2006) "The gatekeeper residue controls autoactivation of ERK2 via a pathway of intramolecular connectivity", Proceedings of the National Academy of Sciences of the United States of America, 103, 18101-6

Emrick MA et al. (2001) "Constitutive activation of extracellular signal-regulated kinase 2 by synergistic point mutations", The Journal of biological chemistry, 276, 46469-79

Autoinhibited structure

Activated structure

144 structures for P28482

Entry ID	Method	Resolution	Chain	Position	Source
1PME	X-ray	200 A	A	1-360	PDB
1TVO	X-ray	250 A	A	1-360	PDB
1WZY	X-ray	250 A	A	1-360	PDB
2OJG	X-ray	200 A	A	2-360	PDB
2OJI	X-ray	260 A	A	2-360	PDB
2OJJ	X-ray	240 A	A	2-360	PDB
2Y9Q	X-ray	155 A	A	1-360	PDB
3D42	X-ray	246 A	B	184-191	PDB
3D44	X-ray	190 A	B	184-191	PDB
3I5Z	X-ray	220 A	A	1-360	PDB
3I60	X-ray	250 A	A	1-360	PDB
3SA0	X-ray	159 A	A	1-360	PDB
3TEI	X-ray	240 A	A	1-360	PDB
3W55	X-ray	300 A	A	1-360	PDB
4FMQ	X-ray	210 A	A	1-360	PDB
4FUX	X-ray	220 A	A	1-360	PDB
4FUY	X-ray	200 A	A	1-360	PDB
4FV0	X-ray	210 A	A	1-360	PDB
4FV1	X-ray	199 A	A	1-360	PDB
4FV2	X-ray	200 A	A	1-360	PDB
4FV3	X-ray	220 A	A	1-360	PDB
4FV4	X-ray	250 A	A	1-360	PDB
4FV5	X-ray	240 A	A	1-360	PDB
4FV6	X-ray	250 A	A	1-360	PDB
4FV7	X-ray	190 A	A	1-360	PDB
4FV8	X-ray	200 A	A	1-360	PDB
4FV9	X-ray	211 A	A	1-360	PDB
4G6N	X-ray	200 A	A	1-360	PDB
4G6O	X-ray	220 A	A	1-360	PDB
4H3P	X-ray	230 A	A/D	1-360	PDB
4H3Q	X-ray	220 A	A	1-360	PDB
4IZ5	X-ray	319 A	A/B/C/D	8-360	PDB
4IZ7	X-ray	180 A	A/C	8-360	PDB
4IZA	X-ray	193 A	A/C	8-360	PDB
4N0S	X-ray	180 A	A	1-360	PDB
4NIF	X-ray	215 A	B/E	1-360	PDB
4O6E	X-ray	195 A	A	13-360	PDB
4QP1	X-ray	270 A	A/B	1-360	PDB
4QP2	X-ray	223 A	A/B	1-360	PDB
4QP3	X-ray	260 A	A/B	1-360	PDB
4QP4	X-ray	220 A	A/B	1-360	PDB
4QP6	X-ray	310 A	A/B	1-360	PDB
4QP7	X-ray	225 A	A/B	1-360	PDB
4QP8	X-ray	245 A	A/B	1-360	PDB
4QP9	X-ray	200 A	A	1-360	PDB
4QPA	X-ray	285 A	A/B	1-360	PDB
4QTA	X-ray	145 A	A	1-360	PDB
4QTE	X-ray	150 A	A	1-360	PDB
4XJ0	X-ray	258 A	A/B	12-360	PDB
4ZXT	X-ray	200 A	A	1-360	PDB
4ZZM	X-ray	189 A	A	11-360	PDB
4ZZN	X-ray	133 A	A	11-360	PDB
4ZZO	X-ray	163 A	A	11-360	PDB
5AX3	X-ray	298 A	A	1-360	PDB
5BUE	X-ray	240 A	A	2-360	PDB
5BUI	X-ray	212 A	A	2-360	PDB
5BUJ	X-ray	185 A	A	2-360	PDB
5BVD	X-ray	190 A	A	2-360	PDB
5BVE	X-ray	200 A	A	2-360	PDB
5BVF	X-ray	190 A	A	2-360	PDB
5K4I	X-ray	176 A	A	9-360	PDB
5LCJ	X-ray	178 A	A	1-360	PDB
5LCK	X-ray	189 A	A	1-360	PDB
5NGU	X-ray	274 A	A	1-360	PDB
5NHF	X-ray	214 A	A	1-360	PDB
5NHH	X-ray	194 A	A	1-360	PDB
5NHJ	X-ray	212 A	A	1-360	PDB
5NHL	X-ray	207 A	A	1-360	PDB
5NHO	X-ray	224 A	A	1-360	PDB
5NHP	X-ray	199 A	A	1-360	PDB
5NHV	X-ray	200 A	A	1-360	PDB
5V60	X-ray	218 A	A	8-360	PDB
5V61	X-ray	220 A	A	8-360	PDB
5V62	X-ray	190 A	A	10-360	PDB
5WP1	X-ray	140 A	A	4-360	PDB
6D5Y	X-ray	286 A	A	13-360	PDB
6DMG	X-ray	220 A	A	11-357	PDB
6G54	X-ray	205 A	A	1-360	PDB
6G8X	X-ray	176 A	A	1-360	PDB
6G91	X-ray	180 A	A	1-360	PDB
6G92	X-ray	199 A	A	1-360	PDB
6G93	X-ray	167 A	A	1-360	PDB
6G97	X-ray	190 A	A	1-360	PDB
6G9A	X-ray	191 A	A	1-360	PDB
6G9D	X-ray	180 A	A	1-360	PDB
6G9H	X-ray	173 A	A	1-360	PDB
6G9J	X-ray	198 A	A	1-360	PDB
6G9K	X-ray	194 A	A	1-360	PDB
6G9M	X-ray	186 A	A	1-360	PDB
6G9N	X-ray	176 A	A	1-360	PDB
6GDM	X-ray	191 A	A	1-360	PDB
6GDQ	X-ray	186 A	A	1-360	PDB
6GE0	X-ray	182 A	A	1-360	PDB
6GJB	X-ray	182 A	A	1-360	PDB
6GJD	X-ray	158 A	A	1-360	PDB
6NBS	X-ray	190 A	A	9-360	PDB
6OPG	X-ray	290 A	A	8-360	PDB
6OPH	X-ray	240 A	A	8-360	PDB
6OPI	X-ray	300 A	A	8-360	PDB
6Q7K	X-ray	184 A	A	1-360	PDB
6Q7S	X-ray	173 A	A	1-360	PDB
6Q7T	X-ray	160 A	A	1-360	PDB
6QA1	X-ray	158 A	A	1-360	PDB
6QA3	X-ray	157 A	A	1-360	PDB
6QA4	X-ray	160 A	A	1-360	PDB
6QAG	X-ray	207 A	A	1-360	PDB
6QAH	X-ray	158 A	A	1-360	PDB
6QAL	X-ray	157 A	A	1-360	PDB
6QAQ	X-ray	158 A	A	1-360	PDB
6QAW	X-ray	184 A	A	1-360	PDB
6RQ4	X-ray	196 A	A	1-360	PDB
6SLG	X-ray	133 A	A	1-360	PDB
7AUV	X-ray	176 A	A	1-360	PDB
7E73	X-ray	228 A	A	1-360	PDB
7E75	X-ray	248 A	A	1-360	PDB
7NQQ	X-ray	194 A	A	1-360	PDB
7NQW	X-ray	177 A	A	1-360	PDB
7NR3	X-ray	190 A	A	1-360	PDB
7NR5	X-ray	177 A	A	1-360	PDB
7NR8	X-ray	163 A	A	1-360	PDB
7NR9	X-ray	191 A	A	1-360	PDB
7OPM	X-ray	245 A	A	1-360	PDB
7W5O	X-ray	235 A	A/B	1-360	PDB
7X4U	X-ray	198 A	A	1-360	PDB
7XC1	X-ray	209 A	A/B	1-360	PDB
8AO2	X-ray	180 A	A	1-360	PDB
8AO3	X-ray	178 A	A	1-360	PDB
8AO4	X-ray	182 A	A	1-360	PDB
8AO5	X-ray	159 A	A	1-360	PDB
8AO6	X-ray	181 A	A	1-360	PDB
8AO7	X-ray	161 A	A	1-360	PDB
8AO8	X-ray	170 A	A	1-360	PDB
8AO9	X-ray	162 A	A	1-360	PDB
8AOA	X-ray	162 A	A	1-360	PDB
8AOB	X-ray	162 A	A	1-360	PDB
8AOC	X-ray	162 A	A	1-360	PDB
8AOD	X-ray	162 A	A	1-360	PDB
8AOE	X-ray	169 A	A	1-360	PDB
8AOF	X-ray	161 A	A	1-360	PDB
8AOG	X-ray	160 A	A	1-360	PDB
8AOH	X-ray	160 A	A	1-360	PDB
8AOI	X-ray	160 A	A	1-360	PDB
8AOJ	X-ray	112 A	A	1-360	PDB
AF-P28482-F1	Predicted				AlphaFoldDB

95 variants for P28482

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
RCV001261413 RCV001264762 rs2069154121	74	I>N	Noonan syndrome 13 Microcephaly [ClinVar]	Yes	ClinVar dbSNP
RCV001261414 rs2069154005	80	H>Y	Microcephaly [ClinVar]	Yes	ClinVar dbSNP
rs797044892 CA204703 RCV000190714	135	R>T	Inborn genetic diseases [ClinVar]	Yes	ClinGen ClinVar Ensembl dbSNP
rs2069052012 RCV001261415 RCV001264763	174	A>V	Heart, malformation of Noonan syndrome 13 [ClinVar]	Yes	ClinVar dbSNP
rs2068716907 RCV001264765 RCV001261417	318	D>G	Heart, malformation of Noonan syndrome 13 [ClinVar]	Yes	ClinVar dbSNP
RCV001261416 rs2068716940 RCV001264764	318	D>N	Heart, malformation of Noonan syndrome 13 [ClinVar]	Yes	ClinVar dbSNP
rs1057519911 RCV000433293 COSM461148 CA16602885 RCV000424324 RCV000442294	322	E>K	cervix Squamous cell carcinoma of the head and neck upper_aerodigestive_tract oesophagus Neoplasm of uterine cervix urinary_tract [Cosmic, ClinVar]	Yes	ClinGen cosmic curated ClinVar Ensembl dbSNP
RCV001261418 rs1057519911	322	E>Q	Microcephaly [ClinVar]	Yes	ClinVar dbSNP
RCV001261419 rs2068678455	323	P>R	Heart, malformation of [ClinVar]	Yes	ClinVar dbSNP
rs1378179068 CA410865731	3	A>E		No	ClinGen TOPMed
CA410865711 rs1282708145	5	A>G		No	ClinGen TOPMed
rs1282708145 CA410865709	5	A>V		No	ClinGen TOPMed
CA322146507 rs977423767	11	P>A		No	ClinGen TOPMed
CA410865649 rs1165567498	11	P>L		No	ClinGen TOPMed
CA410865622 rs1349430059	14	V>I		No	ClinGen TOPMed
CA410865599 rs1213398032	16	G>W		No	ClinGen gnomAD
rs1333567613 CA410865593	17	Q>K		No	ClinGen TOPMed
rs768479151 CA410865578	18	V>L		No	ClinGen ExAC TOPMed gnomAD
rs768479151 CA10124808	18	V>L		No	ClinGen ExAC TOPMed gnomAD
rs867115970 CA322146498	20	D>E		No	ClinGen Ensembl
CA322146495 rs868522804	23	P>Q		No	ClinGen Ensembl
CA410865496 rs1293681277	26	T>A		No	ClinGen gnomAD
CA322146470 rs865964391	33	E>*		No	ClinGen Ensembl
rs868472778 CA322146468	35	A>T		No	ClinGen Ensembl
CA10124781 rs758481470	43	Y>N		No	ClinGen ExAC
rs779189994 CA410867703	44	D>N		No	ClinGen ExAC TOPMed gnomAD
rs779189994 CA10124779	44	D>Y		No	ClinGen ExAC TOPMed gnomAD
rs1008537544 CA322128244	45	N>S		No	ClinGen TOPMed
rs755253671 CA10124778	47	N>D		No	ClinGen ExAC gnomAD
rs754193785 CA10124777	50	R>Q		No	ClinGen ExAC gnomAD
CA410867583 rs1459279170	61	H>P		No	ClinGen TOPMed
rs147916567 CA322128238 COSM110008	63	T>I	skin [Cosmic]	No	ClinGen cosmic curated Ensembl
rs756644901 CA10124775	65	C>S		No	ClinGen ExAC
CA322128231 rs1035981670	72	I>V		No	ClinGen TOPMed
CA410867480 rs761386086	76	L>V		No	ClinGen ExAC TOPMed gnomAD
rs1330050442 CA410867476	77	R>G		No	ClinGen gnomAD
CA410867400 rs1163552201	87	N>S		No	ClinGen TOPMed
CA10124771 rs202041676	94	T>A		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA10124770 rs141978504	94	T>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA410867352 rs1396992418	95	I>V		No	ClinGen gnomAD
rs530183395 CA10124768	96	E>K		No	ClinGen 1000Genomes ExAC gnomAD
rs530183395 CA10124767	96	E>Q		No	ClinGen 1000Genomes ExAC gnomAD
CA10124766 rs146182599	97	Q>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA10124748 rs368100872	106	D>G		No	ClinGen ESP ExAC gnomAD
CA410867200 rs1284302844	113	Y>*		No	ClinGen TOPMed gnomAD
CA410867094 rs1234816629	128	Y>H		No	ClinGen TOPMed
rs1453621955 CA410867054	133	I>V		No	ClinGen gnomAD
CA410867033 rs1569090943	136	G>E		No	ClinGen Ensembl
CA10124739 rs749425404	148	R>H		No	ClinGen ExAC TOPMed gnomAD
rs1166008722 CA410866894	157	L>F		No	ClinGen TOPMed
CA410866853 rs1209985542	163	L>V		No	ClinGen gnomAD
CA10124718 rs746109073	177	D>N		No	ClinGen ExAC gnomAD
rs1282657018 CA410866718	180	H>L		No	ClinGen TOPMed
CA410866662 rs1210556853	189	A>T		No	ClinGen TOPMed
rs1347722713 CA410866624	194	R>T		No	ClinGen gnomAD
CA410866559 rs1334266592	203	K>R		No	ClinGen gnomAD
rs757159464 CA10124685	211	I>T		No	ClinGen ExAC gnomAD
rs865976466 CA322122584	216	C>Y		No	ClinGen Ensembl
CA10124684 rs751520333	223	S>C		No	ClinGen ExAC gnomAD
rs1387475446 CA410864479	231	K>R		No	ClinGen TOPMed
rs1019839970 CA322122541	250	E>D		No	ClinGen TOPMed gnomAD
rs757106100 CA10124666	252	L>M		No	ClinGen ExAC gnomAD
CA10124665 rs751468991	253	N>S		No	ClinGen ExAC gnomAD
rs375334289 CA322122540	255	I>V		No	ClinGen ESP TOPMed gnomAD
rs758368449 CA10124663	258	L>I		No	ClinGen ExAC gnomAD
rs1398062479 CA410863872	271	N>D		No	ClinGen TOPMed
rs751651383 CA322122537	271	N>T		No	ClinGen Ensembl
rs202066163 CA322122535	279	F>V		No	ClinGen Ensembl
rs767782641 CA10124658	280	P>A		No	ClinGen ExAC gnomAD
CA10124659 rs767782641	280	P>S		No	ClinGen ExAC gnomAD
rs762158416 CA10124657	284	S>P		No	ClinGen ExAC gnomAD
CA410863662 rs1368414854	285	K>N		No	ClinGen TOPMed
CA10124636 rs147414870	290	L>V		No	ClinGen ESP ExAC TOPMed gnomAD
CA322355224 rs142890870	292	K>Q		No	ClinGen ESP
CA410833398 rs371304428	296	F>L		No	ClinGen ESP ExAC gnomAD
rs1289443802 CA410833393	297	N>S		No	ClinGen gnomAD
CA410833388 rs1406012731	298	P>A		No	ClinGen TOPMed
rs1156690178 CA410833382	299	H>N		No	ClinGen gnomAD
rs1412049449 CA410833371	300	K>R		No	ClinGen gnomAD
CA10124632 rs776295601	302	I>T		No	ClinGen ExAC gnomAD
rs1380688999 CA410833331	306	Q>E		No	ClinGen Ensembl
CA410833309 rs1400349762	309	A>G		No	ClinGen gnomAD
CA410833313 rs1156943906	309	A>T		No	ClinGen gnomAD
CA322355139 rs866604606	311	P>L		No	ClinGen Ensembl
CA410833253 rs1159689278	317	Y>C		No	ClinGen gnomAD
rs1569079002 CA410833238	319	P>L		No	ClinGen Ensembl
rs554673521 CA10124608	325	A>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA10124606 rs749195559	327	A>S		No	ClinGen ExAC gnomAD
rs1265234526 CA410833167	328	P>S		No	ClinGen gnomAD
CA10124603 rs747124623	332	D>N		No	ClinGen ExAC gnomAD
rs1438987796 CA410832896	350	E>K		No	ClinGen gnomAD
rs1390282714 CA410832869	352	A>S		No	ClinGen gnomAD
CA410832871 rs1390282714	352	A>T		No	ClinGen gnomAD
rs755433157 CA10124598	355	Q>L		No	ClinGen ExAC gnomAD
rs767047236 CA10124596	360	S>Y		No	ClinGen ExAC gnomAD

1 associated diseases with P28482

[MIM: 619087]: Noonan syndrome 13 (NS13)

A form of Noonan syndrome, a disease characterized by short stature, facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears, and a high incidence of congenital heart defects and hypertrophic cardiomyopathy. Other features can include a short neck with webbing or redundancy of skin, deafness, motor delay, variable intellectual deficits, multiple skeletal defects, cryptorchidism, and bleeding diathesis. Individuals with Noonan syndrome are at risk of juvenile myelomonocytic leukemia, a myeloproliferative disorder characterized by excessive production of myelomonocytic cells. NS13 inheritance is autosomal dominant. There is considerable variability in severity. {ECO:0000269|PubMed:32721402}. Note=The disease is caused by variants affecting the gene represented in this entry.

Without disease ID

A form of Noonan syndrome, a disease characterized by short stature, facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears, and a high incidence of congenital heart defects and hypertrophic cardiomyopathy. Other features can include a short neck with webbing or redundancy of skin, deafness, motor delay, variable intellectual deficits, multiple skeletal defects, cryptorchidism, and bleeding diathesis. Individuals with Noonan syndrome are at risk of juvenile myelomonocytic leukemia, a myeloproliferative disorder characterized by excessive production of myelomonocytic cells. NS13 inheritance is autosomal dominant. There is considerable variability in severity. {ECO:0000269|PubMed:32721402}. Note=The disease is caused by variants affecting the gene represented in this entry.

4 regional properties for P28482

Type	Name	Position	InterPro Accession
domain	Protein kinase domain	25 - 313	IPR000719
conserved_site	Mitogen-activated protein (MAP) kinase, conserved site	59 - 161	IPR003527
active_site	Serine/threonine-protein kinase, active site	145 - 157	IPR008271
binding_site	Protein kinase, ATP binding site	31 - 55	IPR017441

Functions

		Description
EC Number	2.7.11.24	Protein-serine/threonine kinases
Subcellular Localization	Cytoplasm, cytoskeleton, spindle Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm Membrane, caveola Cell junction, focal adhesion	Associated with the spindle during prometaphase and metaphase (By similarity) PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization
PANTHER Family	PTHR24055	MITOGEN-ACTIVATED PROTEIN KINASE
PANTHER Subfamily	PTHR24055:SF599	MITOGEN-ACTIVATED PROTEIN KINASE 1
PANTHER Protein Class	non-receptor serine/threonine protein kinase protein modifying enzyme
PANTHER Pathway Category	CCKR signaling map MAPK1 CCKR signaling map MAPK1/3 EGF receptor signaling pathway ERK1-2 B cell activation ERK VEGF signaling pathway MEK Toll receptor signaling pathway ERK2 Interleukin signaling pathway ERK PDGF signaling pathway ERK FGF signaling pathway ERK1-2 Angiotensin II-stimulated signaling through G proteins and beta-arrestin MAPK Interferon-gamma signaling pathway Serine kinase Angiogenesis Erk VEGF signaling pathway Erk Apoptosis signaling pathway MAPK Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade ERK T cell activation ERK Integrin signalling pathway ERK Angiogenesis MEK Gonadotropin-releasing hormone receptor pathway ERK1/2 Ras Pathway ERK Inflammation mediated by chemokine and cytokine signaling pathway MAPK Alzheimer disease-amyloid secretase pathway MAPK TGF-beta signaling pathway p42/p44MAPKs Parkinson disease ERK Endothelin signaling pathway ERK

20 GO annotations of cellular component

Name	Definition
azurophil granule lumen	The volume enclosed by the membrane of an azurophil granule, a primary lysosomal granule found in neutrophil granulocytes that contains a wide range of hydrolytic enzymes and is released into the extracellular fluid.
caveola	A membrane raft that forms small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. Examples include flask-shaped invaginations of the plasma membrane in adipocytes associated with caveolin proteins, and minute pits or incuppings of the cell membrane formed during pinocytosis. Caveolae may be pinched off to form free vesicles within the cytoplasm.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoskeleton	A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
early endosome	A membrane-bounded organelle that receives incoming material from primary endocytic vesicles that have been generated by clathrin-dependent and clathrin-independent endocytosis; vesicles fuse with the early endosome to deliver cargo for sorting into recycling or degradation pathways.
endoplasmic reticulum lumen	The volume enclosed by the membranes of the endoplasmic reticulum.
extracellular region	The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
ficolin-1-rich granule lumen	Any membrane-enclosed lumen that is part of a ficolin-1-rich granule.
focal adhesion	A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
Golgi apparatus	A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
late endosome	A prelysosomal endocytic organelle differentiated from early endosomes by lower lumenal pH and different protein composition. Late endosomes are more spherical than early endosomes and are mostly juxtanuclear, being concentrated near the microtubule organizing center.
microtubule organizing center	An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
mitotic spindle	A spindle that forms as part of mitosis. Mitotic and meiotic spindles contain distinctive complements of proteins associated with microtubules.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
pseudopodium	A temporary protrusion or retractile process of a cell, associated with flowing movements of the protoplasm, and serving for locomotion and feeding.
synapse	The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane.

10 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
DNA binding	Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
identical protein binding	Binding to an identical protein or proteins.
MAP kinase activity	Catalysis of the reaction: protein + ATP = protein phosphate + ADP. This reaction is the phosphorylation of proteins. Mitogen-activated protein kinase; a family of protein kinases that perform a crucial step in relaying signals from the plasma membrane to the nucleus. They are activated by a wide range of proliferation- or differentiation-inducing signals; activation is strong with agonists such as polypeptide growth factors and tumor-promoting phorbol esters, but weak (in most cell backgrounds) by stress stimuli.
MAP kinase kinase activity	Catalysis of the concomitant phosphorylation of threonine (T) and tyrosine (Y) residues in a Thr-Glu-Tyr (TEY) thiolester sequence in a MAP kinase (MAPK) substrate.
phosphatase binding	Binding to a phosphatase.
phosphotyrosine residue binding	Binding to a phosphorylated tyrosine residue within a protein.
protein serine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
RNA polymerase II CTD heptapeptide repeat kinase activity	Catalysis of the reaction: ATP + RNA polymerase II large subunit CTD heptapeptide repeat (YSPTSPS) = ADP + H+ + phosphorylated RNA polymerase II.

55 GO annotations of biological process

Name	Definition
apoptotic process	A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
B cell receptor signaling pathway	The series of molecular signals initiated by the cross-linking of an antigen receptor on a B cell.
Bergmann glial cell differentiation	The process in which neuroepithelial cells of the neural tube give rise to Brgmann glial cells, specialized bipotential progenitors cells of the cerebellum. Differentiation includes the processes involved in commitment of a cell to a specific fate.
cardiac neural crest cell development involved in heart development	The process aimed at the progression of a cardiac neural crest cell over time, from initial commitment of the cell to its specific fate, to the fully functional differentiated cell that contributes to the development of the heart.
caveolin-mediated endocytosis	An endocytosis process that begins when material is taken up into plasma membrane caveolae, which then pinch off to form endocytic caveolar carriers.
cell cycle	The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
cell surface receptor signaling pathway	The series of molecular signals initiated by activation of a receptor on the surface of a cell. The pathway begins with binding of an extracellular ligand to a cell surface receptor, or for receptors that signal in the absence of a ligand, by ligand-withdrawal or the activity of a constitutively active receptor. The pathway ends with regulation of a downstream cellular process, e.g. transcription.
cellular response to amino acid starvation	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of amino acids.
cellular response to cadmium ion	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.
cellular response to DNA damage stimulus	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
cellular response to dopamine	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a dopamine stimulus.
cellular response to granulocyte macrophage colony-stimulating factor stimulus	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a granulocyte macrophage colony-stimulating factor stimulus.
cellular response to reactive oxygen species	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a reactive oxygen species stimulus. Reactive oxygen species include singlet oxygen, superoxide, and oxygen free radicals.
cellular response to tumor necrosis factor	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a tumor necrosis factor stimulus.
chemical synaptic transmission	The vesicular release of classical neurotransmitter molecules from a presynapse, across a chemical synapse, the subsequent activation of neurotransmitter receptors at the postsynapse of a target cell (neuron, muscle, or secretory cell) and the effects of this activation on the postsynaptic membrane potential and ionic composition of the postsynaptic cytosol. This process encompasses both spontaneous and evoked release of neurotransmitter and all parts of synaptic vesicle exocytosis. Evoked transmission starts with the arrival of an action potential at the presynapse.
chemotaxis	The directed movement of a motile cell or organism, or the directed growth of a cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis).
cytosine metabolic process	The chemical reactions and pathways involving cytosine, 4-amino-2-hydroxypyrimidine, a pyrimidine derivative that is one of the five main bases found in nucleic acids; it occurs widely in cytidine derivatives.
ERBB signaling pathway	The series of molecular signals initiated by binding of a ligand to a member of the ERBB family of receptor tyrosine kinases on the surface of a cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
ERK1 and ERK2 cascade	An intracellular protein kinase cascade containing at least ERK1 or ERK2 (MAPKs), a MEK (a MAPKK) and a MAP3K. The cascade may involve 4 different kinases, as it can also contain an additional tier: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell.
face development	The biological process whose specific outcome is the progression of a face from an initial condition to its mature state. The face is the ventral division of the head.
intracellular signal transduction	The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
labyrinthine layer blood vessel development	The process whose specific outcome is the progression of a blood vessel of the labyrinthine layer of the placenta over time, from its formation to the mature structure. The embryonic vessels grow through the layer to come in close contact with the maternal blood supply.
learning or memory	The acquisition and processing of information and/or the storage and retrieval of this information over time.
lipopolysaccharide-mediated signaling pathway	The series of molecular signals initiated by the binding of a lipopolysaccharide (LPS) to a receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. Lipopolysaccharides are major components of the outer membrane of Gram-negative bacteria, making them prime targets for recognition by the immune system.
long-term synaptic potentiation	A process that modulates synaptic plasticity such that synapses are changed resulting in the increase in the rate, or frequency of synaptic transmission at the synapse.
lung morphogenesis	The process in which the anatomical structures of the lung are generated and organized.
mammary gland epithelial cell proliferation	The multiplication or reproduction of mammary gland epithelial cells, resulting in the expansion of a cell population. Mammary gland epithelial cells make up the covering of surfaces of the mammary gland. The mammary gland is a large compound sebaceous gland that in female mammals is modified to secrete milk.
negative regulation of cell differentiation	Any process that stops, prevents, or reduces the frequency, rate or extent of cell differentiation.
outer ear morphogenesis	The process in which the anatomical structures of the outer ear are generated and organized. The outer ear is the part of the ear external to the tympanum (eardrum). It consists of a tube (the external auditory meatus) that directs sound waves on to the tympanum, and may also include the external pinna, which extends beyond the skull.
peptidyl-serine phosphorylation	The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
peptidyl-threonine phosphorylation	The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of gene expression	Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of macrophage chemotaxis	Any process that increases the rate, frequency or extent of macrophage chemotaxis. Macrophage chemotaxis is the movement of a macrophage in response to an external stimulus.
positive regulation of macrophage proliferation	Any process that activates or increases the frequency, rate or extent of macrophage proliferation.
positive regulation of peptidyl-threonine phosphorylation	Any process that increases the frequency, rate or extent of peptidyl-threonine phosphorylation. Peptidyl-threonine phosphorylation is the phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of telomerase activity	Any process that activates or increases the frequency, rate or extent of telomerase activity, the catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
positive regulation of telomere capping	Any process that activates or increases the frequency, rate or extent of telomere capping.
positive regulation of telomere maintenance via telomerase	Any process that activates or increases the frequency, rate or extent of the addition of telomeric repeats by telomerase.
protein phosphorylation	The process of introducing a phosphate group on to a protein.
regulation of cellular pH	Any process involved in the maintenance of an internal equilibrium of hydrogen ions (protons) within a cell or between a cell and its external environment.
regulation of cytoskeleton organization	Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures.
regulation of early endosome to late endosome transport	Any process that modulates the frequency, rate or extent of early endosome to late endosome transport.
regulation of Golgi inheritance	Any process that modulates the rate, frequency or extent of Golgi inheritance. Golgi inheritance is the partitioning of Golgi apparatus between daughter cells at cell division.
regulation of ossification	Any process that modulates the frequency, rate or extent of ossification, the formation of bone or of a bony substance or the conversion of fibrous tissue or of cartilage into bone or a bony substance.
regulation of protein stability	Any process that affects the structure and integrity of a protein, altering the likelihood of its degradation or aggregation.
regulation of stress-activated MAPK cascade	Any process that modulates the frequency, rate or extent of signal transduction mediated by the stress-activated MAPK cascade.
response to epidermal growth factor	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an epidermal growth factor stimulus.
response to exogenous dsRNA	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an exogenous double-stranded RNA stimulus.
response to nicotine	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nicotine stimulus.
signal transduction	The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
stress-activated MAPK cascade	The series of molecular signals in which a stress-activated MAP kinase cascade relays a signal; MAP kinase cascades involve at least three protein kinase activities and culminate in the phosphorylation and activation of a MAP kinase.
T cell receptor signaling pathway	The series of molecular signals initiated by the cross-linking of an antigen receptor on a T cell.
thymus development	The process whose specific outcome is the progression of the thymus over time, from its formation to the mature structure. The thymus is a symmetric bi-lobed organ involved primarily in the differentiation of immature to mature T cells, with unique vascular, nervous, epithelial, and lymphoid cell components.
thyroid gland development	The process whose specific outcome is the progression of the thyroid gland over time, from its formation to the mature structure. The thyroid gland is an endoderm-derived gland that produces thyroid hormone.
trachea formation	The process pertaining to the initial formation of a trachea from unspecified parts. The process begins with the specific processes that contribute to the appearance of the discrete structure and ends when the trachea is recognizable. The trachea is the portion of the airway that attaches to the bronchi as it branches.

92 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P16892	FUS3	Mitogen-activated protein kinase FUS3	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
P14681	KSS1	Mitogen-activated protein kinase KSS1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
A5PKJ4	MAPK7	Mitogen-activated protein kinase 7	Bos taurus (Bovine)	SS
Q3T0N5	MAPK13	Mitogen-activated protein kinase 13	Bos taurus (Bovine)	SS
P46196	MAPK1	Mitogen-activated protein kinase 1	Bos taurus (Bovine)	SS
Q5F3W3	MAPK6	Mitogen-activated protein kinase 6	Gallus gallus (Chicken)	SS
Q95NE7	MAPK14	Mitogen-activated protein kinase 14	Pan troglodytes (Chimpanzee)	SS
Q9N272	MAPK13	Mitogen-activated protein kinase 13	Pan troglodytes (Chimpanzee)	SS
Q9W354	Erk7	Extracellular signal-regulated kinase 7	Drosophila melanogaster (Fruit fly)	SS
O61443	p38b	Mitogen-activated protein kinase p38b	Drosophila melanogaster (Fruit fly)	SS
O62618	p38a	Mitogen-activated protein kinase p38a	Drosophila melanogaster (Fruit fly)	SS
P83100	p38c	Putative mitogen-activated protein kinase 14C	Drosophila melanogaster (Fruit fly)	SS
P40417	rl	Mitogen-activated protein kinase ERK-A	Drosophila melanogaster (Fruit fly)	SS
P20794	MAK	Serine/threonine-protein kinase MAK	Homo sapiens (Human)	PR
Q9UQ07	MOK	MAPK/MAK/MRK overlapping kinase	Homo sapiens (Human)	PR
Q8TD08	MAPK15	Mitogen-activated protein kinase 15	Homo sapiens (Human)	SS
P45984	MAPK9	Mitogen-activated protein kinase 9	Homo sapiens (Human)	SS
P45983	MAPK8	Mitogen-activated protein kinase 8	Homo sapiens (Human)	EV
P53779	MAPK10	Mitogen-activated protein kinase 10	Homo sapiens (Human)	EV
Q9UBE8	NLK	Serine/threonine-protein kinase NLK	Homo sapiens (Human)	SS
P27361	MAPK3	Mitogen-activated protein kinase 3	Homo sapiens (Human)	SS
P31152	MAPK4	Mitogen-activated protein kinase 4	Homo sapiens (Human)	SS
Q16659	MAPK6	Mitogen-activated protein kinase 6	Homo sapiens (Human)	SS
Q13164	MAPK7	Mitogen-activated protein kinase 7	Homo sapiens (Human)	SS
P53778	MAPK12	Mitogen-activated protein kinase 12	Homo sapiens (Human)	SS
O15264	MAPK13	Mitogen-activated protein kinase 13	Homo sapiens (Human)	SS
Q15759	MAPK11	Mitogen-activated protein kinase 11	Homo sapiens (Human)	SS
Q16539	MAPK14	Mitogen-activated protein kinase 14	Homo sapiens (Human)	SS
Q9Z1B7	Mapk13	Mitogen-activated protein kinase 13	Mus musculus (Mouse)	SS
P47811	Mapk14	Mitogen-activated protein kinase 14	Mus musculus (Mouse)	SS
Q61532	Mapk6	Mitogen-activated protein kinase 6	Mus musculus (Mouse)	SS
Q9WVS8	Mapk7	Mitogen-activated protein kinase 7	Mus musculus (Mouse)	SS
Q9WUI1	Mapk11	Mitogen-activated protein kinase 11	Mus musculus (Mouse)	SS
O08911	Mapk12	Mitogen-activated protein kinase 12	Mus musculus (Mouse)	SS
Q80Y86	Mapk15	Mitogen-activated protein kinase 15	Mus musculus (Mouse)	SS
Q6P5G0	Mapk4	Mitogen-activated protein kinase 4	Mus musculus (Mouse)	SS
Q63844	Mapk3	Mitogen-activated protein kinase 3	Mus musculus (Mouse)	SS
P63085	Mapk1	Mitogen-activated protein kinase 1	Mus musculus (Mouse)	SS
P70618	Mapk14	Mitogen-activated protein kinase 14	Rattus norvegicus (Rat)	SS
Q9WTY9	Mapk13	Mitogen-activated protein kinase 13	Rattus norvegicus (Rat)	SS
Q9Z2A6	Mapk15	Mitogen-activated protein kinase 15	Rattus norvegicus (Rat)	SS
P27704	Mapk6	Mitogen-activated protein kinase 6	Rattus norvegicus (Rat)	SS
Q63538	Mapk12	Mitogen-activated protein kinase 12	Rattus norvegicus (Rat)	SS
Q63454	Mapk4	Mitogen-activated protein kinase 4	Rattus norvegicus (Rat)	SS
P0C865	Mapk7	Mitogen-activated protein kinase 7	Rattus norvegicus (Rat)	SS
P21708	Mapk3	Mitogen-activated protein kinase 3	Rattus norvegicus (Rat)	SS
P63086	Mapk1	Mitogen-activated protein kinase 1	Rattus norvegicus (Rat)	SS
Q5ZCI1	MPK10	Mitogen-activated protein kinase 10	Oryza sativa subsp. japonica (Rice)	SS
Q5VP69	MPK16	Mitogen-activated protein kinase 16	Oryza sativa subsp. japonica (Rice)	SS
Q5SN53	MPK8	Mitogen-activated protein kinase 8	Oryza sativa subsp. japonica (Rice)	SS
Q0E459	MPK13	Mitogen-activated protein kinase 13	Oryza sativa subsp. japonica (Rice)	SS
Q75KK8	MPK14	Mitogen-activated protein kinase 14	Oryza sativa subsp. japonica (Rice)	SS
Q67C40	MPK7	Mitogen-activated protein kinase 7	Oryza sativa subsp. japonica (Rice)	SS
Q6L5F7	MPK17	Mitogen-activated protein kinase 17	Oryza sativa subsp. japonica (Rice)	SS
Q5VN19	MPK11	Mitogen-activated protein kinase 11	Oryza sativa subsp. japonica (Rice)	SS
Q5Z9J0	MPK12	Mitogen-activated protein kinase 12	Oryza sativa subsp. japonica (Rice)	SS
Q53N72	MPK15	Mitogen-activated protein kinase 15	Oryza sativa subsp. japonica (Rice)	SS
Q5Z859	MPK4	Mitogen-activated protein kinase 4	Oryza sativa subsp. japonica (Rice)	SS
Q6Z437	MPK3	Mitogen-activated protein kinase 3	Oryza sativa subsp. japonica (Rice)	SS
Q336X9	MPK6	Mitogen-activated protein kinase 6	Oryza sativa subsp. japonica (Rice)	SS
Q5J4W4	MPK2	Mitogen-activated protein kinase 2	Oryza sativa subsp. japonica (Rice)	SS
Q84UI5	MPK1	Mitogen-activated protein kinase 1	Oryza sativa subsp. japonica (Rice)	SS
Q10N20	MPK5	Mitogen-activated protein kinase 5	Oryza sativa subsp. japonica (Rice)	SS
Q17446	pmk-1	Mitogen-activated protein kinase pmk-1	Caenorhabditis elegans	SS
Q11179	mapk-15	Mitogen-activated protein kinase 15	Caenorhabditis elegans	SS
Q8MXI4	pmk-2	Mitogen-activated protein kinase pmk-2	Caenorhabditis elegans	SS
O44514	pmk-3	Mitogen-activated protein kinase pmk-3	Caenorhabditis elegans	PR
G5EBT1	sma-5	Mitogen-activated protein kinase sma-5	Caenorhabditis elegans	SS
P39745	mpk-1	Mitogen-activated protein kinase mpk-1	Caenorhabditis elegans	SS
Q9C9U4	MPK15	Mitogen-activated protein kinase 15	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9LV37	MPK9	Mitogen-activated protein kinase 9	Arabidopsis thaliana (Mouse-ear cress)	SS
O23236	MPK14	Mitogen-activated protein kinase 14	Arabidopsis thaliana (Mouse-ear cress)	SS
Q39021	MPK1	Mitogen-activated protein kinase 1	Arabidopsis thaliana (Mouse-ear cress)	SS
Q39022	MPK2	Mitogen-activated protein kinase 2	Arabidopsis thaliana (Mouse-ear cress)	SS
Q39027	MPK7	Mitogen-activated protein kinase 7	Arabidopsis thaliana (Mouse-ear cress)	SS
Q84M93	MPK17	Mitogen-activated protein kinase 17	Arabidopsis thaliana (Mouse-ear cress)	SS
Q8W4J2	MPK16	Mitogen-activated protein kinase 16	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9C5C0	MPK18	Mitogen-activated protein kinase 18	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9LM33	MPK8	Mitogen-activated protein kinase 8	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9LUC3	MPK19	Mitogen-activated protein kinase 19	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9SJG9	MPK20	Mitogen-activated protein kinase 20	Arabidopsis thaliana (Mouse-ear cress)	SS
Q39023	MPK3	Mitogen-activated protein kinase 3	Arabidopsis thaliana (Mouse-ear cress)	SS
Q39026	MPK6	Mitogen-activated protein kinase 6	Arabidopsis thaliana (Mouse-ear cress)	SS
Q39025	MPK5	Mitogen-activated protein kinase 5	Arabidopsis thaliana (Mouse-ear cress)	SS
Q39024	MPK4	Mitogen-activated protein kinase 4	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9M1Z5	MPK10	Mitogen-activated protein kinase 10	Arabidopsis thaliana (Mouse-ear cress)	SS
Q8GYQ5	MPK12	Mitogen-activated protein kinase 12	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9LMM5	MPK11	Mitogen-activated protein kinase 11	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9LQQ9	MPK13	Mitogen-activated protein kinase 13	Arabidopsis thaliana (Mouse-ear cress)	SS
O42376	mapk12	Mitogen-activated protein kinase 12	Danio rerio (Zebrafish) (Brachydanio rerio)	SS
Q9DGE1	mapk14b	Mitogen-activated protein kinase 14B	Danio rerio (Zebrafish) (Brachydanio rerio)	SS
Q9DGE2	mapk14a	Mitogen-activated protein kinase 14A	Danio rerio (Zebrafish) (Brachydanio rerio)	SS

10	20	30	40	50	60
MAAAAAAGAG	PEMVRGQVFD	VGPRYTNLSY	IGEGAYGMVC	SAYDNVNKVR	VAIKKISPFE
70	80	90	100	110	120
HQTYCQRTLR	EIKILLRFRH	ENIIGINDII	RAPTIEQMKD	VYIVQDLMET	DLYKLLKTQH
130	140	150	160	170	180
LSNDHICYFL	YQILRGLKYI	HSANVLHRDL	KPSNLLLNTT	CDLKICDFGL	ARVADPDHDH
190	200	210	220	230	240
TGFLTEYVAT	RWYRAPEIML	NSKGYTKSID	IWSVGCILAE	MLSNRPIFPG	KHYLDQLNHI
250	260	270	280	290	300
LGILGSPSQE	DLNCIINLKA	RNYLLSLPHK	NKVPWNRLFP	NADSKALDLL	DKMLTFNPHK
310	320	330	340	350
RIEVEQALAH	PYLEQYYDPS	DEPIAEAPFK	FDMELDDLPK	EKLKELIFEE	TARFQPGYRS