P27577

EV
Gene name
Ets1 (Ets-1)
Protein name
Protein C-ets-1
Names
p54
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:23871
EC number
Protein Class
ETS (PTHR11849)

Descriptions

Ets1 is a transcription factor directly controlling the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. Truncation of either the N-terminal inhibitory region (residues 280-330) or the C-terminal inhibitory region (residues 416-440) increases the DNA binding affinity of Ets-1 by 10- to 20-fold, indicating that the two inhibitory regions act in concert to impair DNA binding

Autoinhibitory domains (AIDs)

304-310 (Alpha-helix HI-1 in N-terminal inhibitory region) EV

Target domain

331-415 (ETS domain)

Relief mechanism

Partner binding

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

416-440 (C-terminal inhibitory region) EV

Target domain

331-415 (ETS domain)

Relief mechanism

Partner binding

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

11 structures for P27577

Entry ID Method Resolution Chain Position Source
1K78 X-ray 225 A B/F 331-440 PDB
1K79 X-ray 240 A A/D 331-440 PDB
1K7A X-ray 280 A A/D 331-440 PDB
1MD0 X-ray 200 A A/B 300-440 PDB
1MDM X-ray 280 A B 280-440 PDB
1R36 NMR - A 301-440 PDB
2JV3 NMR - A 29-138 PDB
2KMD NMR - A 29-138 PDB
6DA1 X-ray 200 A A/B 301-440 PDB
6DAT X-ray 235 A A/B/C/D 301-440 PDB
AF-P27577-F1 Predicted AlphaFoldDB

1 variants for P27577

Variant ID(s) Position Change Description Diseaes Association Provenance
rs38156610 310 D>E No Ensembl

No associated diseases with P27577

9 regional properties for P27577

Type Name Position InterPro Accession
domain Protein kinase domain 270 - 523 IPR000719
domain SH2 domain 146 - 245 IPR000980
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 270 - 519 IPR001245
domain SH3 domain 81 - 142 IPR001452
active_site Tyrosine-protein kinase, active site 385 - 397 IPR008266
binding_site Protein kinase, ATP binding site 276 - 298 IPR017441
domain Tyrosine-protein kinase, catalytic domain 270 - 519 IPR020635
domain Fyn/Yrk, SH3 domain 84 - 139 IPR035750
domain Fyn/Yrk, SH2 domain 144 - 244 IPR047924

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
  • Cytoplasm
PANTHER Family PTHR11849 ETS
PANTHER Subfamily PTHR11849:SF209 PROTEIN C-ETS-1
PANTHER Protein Class DNA-binding transcription factor
helix-turn-helix transcription factor
winged helix/forkhead transcription factor
PANTHER Pathway Category PDGF signaling pathway
Ets
Ras Pathway
Ets
VEGF signaling pathway
Ets
Angiogenesis
Ets

4 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
transcription regulator complex A protein complex that is capable of associating with DNA by direct binding, or via other DNA-binding proteins or complexes, and regulating transcription.

12 GO annotations of molecular function

Name Definition
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA-binding transcription activator activity, RNA polymerase II-specific A DNA-binding transcription factor activity that activates or increases transcription of specific gene sets transcribed by RNA polymerase II.
DNA-binding transcription factor activity A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
DNA-binding transcription factor activity, RNA polymerase II-specific A DNA-binding transcription factor activity that modulates the transcription of specific gene sets transcribed by RNA polymerase II.
DNA-binding transcription factor binding Binding to a DNA-binding transcription factor, a protein that interacts with a specific DNA sequence (sometimes referred to as a motif) within the regulatory region of a gene to modulate transcription.
histone acetyltransferase binding Binding to an histone acetyltransferase.
identical protein binding Binding to an identical protein or proteins.
nuclear receptor coactivator activity A transcription coactivator activity that activates or increases the transcription of specific gene sets via binding to a DNA-bound nuclear receptor, either on its own or as part of a complex. Coactivators often act by altering chromatin structure and modifications. For example, one class of transcription coregulators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. A fourth class of coactivator activity is the bridging of a DNA-binding transcription factor to the general (basal) transcription machinery. The Mediator complex, which bridges sequence-specific DNA binding transcription factors and RNA polymerase, is also a transcription coactivator.
RNA polymerase II cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II.
sequence-specific DNA binding Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding.
sequence-specific double-stranded DNA binding Binding to double-stranded DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA, e.g. promotor binding or rDNA binding.
transcription cis-regulatory region binding Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon.

25 GO annotations of biological process

Name Definition
cell differentiation The process in which relatively unspecialized cells, e.g. embryonic or regenerative cells, acquire specialized structural and/or functional features that characterize the cells, tissues, or organs of the mature organism or some other relatively stable phase of the organism's life history. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
cell motility Any process involved in the controlled self-propelled movement of a cell that results in translocation of the cell from one place to another.
immune system process Any process involved in the development or functioning of the immune system, an organismal system for calibrated responses to potential internal or invasive threats.
negative regulation of cell cycle Any process that stops, prevents or reduces the rate or extent of progression through the cell cycle.
negative regulation of inflammatory response Any process that stops, prevents, or reduces the frequency, rate or extent of the inflammatory response.
PML body organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of PML bodies, a class of nuclear body; they react against SP100 auto-antibodies (PML = promyelocytic leukemia).
positive regulation of angiogenesis Any process that activates or increases angiogenesis.
positive regulation of blood vessel endothelial cell migration Any process that activates or increases the frequency, rate or extent of the migration of the endothelial cells of blood vessels.
positive regulation of cell migration Any process that activates or increases the frequency, rate or extent of cell migration.
positive regulation of cell population proliferation Any process that activates or increases the rate or extent of cell proliferation.
positive regulation of cellular component movement OBSOLETE. Any process that activates or increases the frequency, rate or extent of the movement of a cellular component.
positive regulation of endothelial cell migration Any process that increases the rate, frequency, or extent of the orderly movement of an endothelial cell into the extracellular matrix to form an endothelium.
positive regulation of erythrocyte differentiation Any process that activates or increases the frequency, rate or extent of erythrocyte differentiation.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of inflammatory response Any process that activates or increases the frequency, rate or extent of the inflammatory response.
positive regulation of leukocyte adhesion to vascular endothelial cell Any process that activates or increases the frequency, rate or extent of leukocyte adhesion to vascular endothelial cell.
positive regulation of pri-miRNA transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of microRNA (miRNA) gene transcription.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
positive regulation of transcription, DNA-templated Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.
regulation of angiogenesis Any process that modulates the frequency, rate or extent of angiogenesis.
regulation of apoptotic process Any process that modulates the occurrence or rate of cell death by apoptotic process.
regulation of extracellular matrix disassembly Any process that modulates the rate, frequency or extent of extracellular matrix disassembly. Extracellular matrix disassembly is a process that results in the breakdown of the extracellular matrix.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to antibiotic Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
transcription by RNA polymerase II The synthesis of RNA from a DNA template by RNA polymerase II (RNAP II), originating at an RNA polymerase II promoter. Includes transcription of messenger RNA (mRNA) and certain small nuclear RNAs (snRNAs).

40 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
A1A4L6 ETS2 Protein C-ets-2 Bos taurus (Bovine) SS
Q2KIC2 ETV1 ETS translocation variant 1 Bos taurus (Bovine) SS
P10157 ETS2 Protein C-ets-2 Gallus gallus (Chicken) SS
Q90837 ERG Transcriptional regulator Erg Gallus gallus (Chicken) SS
P13474 ETS1 Transforming protein p54/c-ets-1 Gallus gallus (Chicken) SS
P15062 ETS1 Transforming protein p68/c-ets-1 Gallus gallus (Chicken) SS
A2T762 ETV3 ETS translocation variant 3 Pan troglodytes (Chimpanzee) PR
Q04688 Ets97D DNA-binding protein Ets97D Drosophila melanogaster (Fruit fly) PR
P50548 ERF ETS domain-containing transcription factor ERF Homo sapiens (Human) PR
P41970 ELK3 ETS domain-containing protein Elk-3 Homo sapiens (Human) SS
P41161 ETV5 ETS translocation variant 5 Homo sapiens (Human) SS
P43268 ETV4 ETS translocation variant 4 Homo sapiens (Human) EV
Q06546 GABPA GA-binding protein alpha chain Homo sapiens (Human) SS
P28324 ELK4 ETS domain-containing protein Elk-4 Homo sapiens (Human) EV
P15036 ETS2 Protein C-ets-2 Homo sapiens (Human) EV
P41162 ETV3 ETS translocation variant 3 Homo sapiens (Human) PR
P50549 ETV1 ETS translocation variant 1 Homo sapiens (Human) EV
P11308 ERG Transcriptional regulator ERG Homo sapiens (Human) EV
P19419 ELK1 ETS domain-containing protein Elk-1 Homo sapiens (Human) EV
P14921 ETS1 Protein C-ets-1 Homo sapiens (Human) EV
P97360 Etv6 Transcription factor ETV6 Mus musculus (Mouse) EV
Q8VDK3 Elf5 ETS-related transcription factor Elf-5 Mus musculus (Mouse) SS
Q60775 Elf1 ETS-related transcription factor Elf-1 Mus musculus (Mouse) PR
Q9Z2U4 Elf4 ETS-related transcription factor Elf-4 Mus musculus (Mouse) PR
Q9JHC9 Elf2 ETS-related transcription factor Elf-2 Mus musculus (Mouse) PR
Q00422 Gabpa GA-binding protein alpha chain Mus musculus (Mouse) EV
Q9WTP3 Spdef SAM pointed domain-containing Ets transcription factor Mus musculus (Mouse) PR
P70459 Erf ETS domain-containing transcription factor ERF Mus musculus (Mouse) PR
P81270 Erg Transcriptional regulator ERG Mus musculus (Mouse) SS
P28322 Etv4 ETS translocation variant 4 Mus musculus (Mouse) SS
Q9CXC9 Etv5 ETS translocation variant 5 Mus musculus (Mouse) SS
P41164 Etv1 ETS translocation variant 1 Mus musculus (Mouse) SS
P41971 Elk3 ETS domain-containing protein Elk-3 Mus musculus (Mouse) EV
P41158 Elk4 ETS domain-containing protein Elk-4 Mus musculus (Mouse) PR
P41969 Elk1 ETS domain-containing protein Elk-1 Mus musculus (Mouse) PR
P15037 Ets2 Protein C-ets-2 Mus musculus (Mouse) SS
A4GTP4 Elk1 ETS domain-containing protein Elk-1 Rattus norvegicus (Rat) PR
P41156 Ets1 Protein C-ets-1 Rattus norvegicus (Rat) SS
Q9PUQ1 etv4 ETS translocation variant 4 Danio rerio (Zebrafish) (Brachydanio rerio) SS
A3FEM2 fev Protein FEV Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MKAAVDLKPT LTIIKTEKVD LELFPSPDME CADVPLLTPS SKEMMSQALK ATFSGFTKEQ
70 80 90 100 110 120
QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM SGAALCALGK ECFLELAPDF
130 140 150 160 170 180
VGDILWEHLE ILQKEDVKPY QVNGANPTYP ESCYTSDYFI SYGIEHAQCV PPSEFSEPSF
190 200 210 220 230 240
ITESYQTLHP ISSEELLSLK YENDYPSVIL QDPLQTDTLQ TDYFAIKQEV LTPDNMCLGR
250 260 270 280 290 300
ASRGKLGGQD SFESVESYDS CDRLTQSWSS QSSFNSLQRV PSYDSFDYED YPAALPNHKP
310 320 330 340 350 360
KGTFKDYVRD RADLNKDKPV IPAAALAGYT GSGPIQLWQF LLELLTDKSC QSFISWTGDG
370 380 390 400 410 420
WEFKLSDPDE VARRWGKRKN KPKMNYEKLS RGLRYYYDKN IIHKTAGKRY VYRFVCDLQS
430
LLGYTPEELH AMLDVKPDAD