P26234

SS
Gene name
VCL
Protein name
Vinculin
Names
Metavinculin
Species
Sus scrofa (Pig)
KEGG Pathway
ssc:396974
EC number
Protein Class

Descriptions

Vinculin is a highly conserved intracellular protein with a crucial role in the maintenance and regulation of cell adhesion and migration. N-terminal globular head (Vh) is composed of subdomains (D1-D4). The C-terminal tail (Vt) binds F-actin. A strap sequence (878-890) between Pro-rich region and Vt packs against the Vt and thereby masks the F-actin-binding domain in Vt.

Autoinhibitory domains (AIDs)

879-891 (Strap sequence) SS

Target domain

897-1067 (Vt domain)

Relief mechanism

Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P26234

Entry ID Method Resolution Chain Position Source
AF-P26234-F1 Predicted AlphaFoldDB

13 variants for P26234

Variant ID(s) Position Change Description Diseaes Association Provenance
rs700041698 263 D>E No Ensembl
rs708246375 330 G>E No Ensembl
rs709375384 447 A>S No Ensembl
rs705936721 511 D>N No Ensembl
rs713946251 541 D>Y No Ensembl
rs711720622 616 A>S No Ensembl
rs702284868 640 R>K No Ensembl
rs700119545 698 T>S No Ensembl
rs704614802 938 V>A No Ensembl
rs712554811 944 S>P No Ensembl
rs696990132 960 S>N No Ensembl
rs712773823 1002 M>T No Ensembl
rs698137713 1032 R>W No Ensembl

No associated diseases with P26234

3 regional properties for P26234

Type Name Position InterPro Accession
conserved_site 14-3-3 protein, conserved site 49 - 59 IPR023409-1
conserved_site 14-3-3 protein, conserved site 221 - 240 IPR023409-2
domain 14-3-3 domain 9 - 252 IPR023410

Functions

Description
EC Number
Subcellular Localization
  • Cell membrane ; Peripheral membrane protein ; Cytoplasmic side
  • Cell junction, adherens junction
  • Cell junction, focal adhesion
  • Cytoplasm, cytoskeleton
  • Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side
  • Cell projection, podosome
  • Recruitment to cell-cell junctions occurs in a myosin II-dependent manner
  • Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

14 GO annotations of cellular component

Name Definition
adherens junction A cell-cell junction composed of the epithelial cadherin-catenin complex. The epithelial cadherins, or E-cadherins, of each interacting cell extend through the plasma membrane into the extracellular space and bind to each other. The E-cadherins bind to catenins on the cytoplasmic side of the membrane, where the E-cadherin-catenin complex binds to cytoskeletal components and regulatory and signaling molecules.
brush border The dense covering of microvilli on the apical surface of an epithelial cell in tissues such as the intestine, kidney, and choroid plexus; the microvilli aid absorption by increasing the surface area of the cell.
cell-cell junction A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects.
costamere Regular periodic sub membranous arrays of vinculin in skeletal and cardiac muscle cells, these arrays link Z-discs to the sarcolemma and are associated with links to extracellular matrix.
fascia adherens A cell-cell junction that contains the transmembrane protein N-cadherin, which interacts with identical molecules from neighbouring cells to form a tight mechanical intercellular link; forms a large portion of the intercalated disc, the structure at which myofibrils terminate in cardiomyocytes.
focal adhesion A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
inner dense plaque of desmosome The desmosomal part containing the C-termini of desmoplakins which interact with the keratin intermediate filaments, serving to tether the intermediate filaments to the plasma membrane.
outer dense plaque of desmosome The desmosomal part containing plakoglobins, plakophilins, the N-termini of desmoplakins, as well as the cytoplasmic tails of the desmosomal cadherins, which together attach the plaque to the plasma membrane.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
podosome An actin-rich adhesion structure characterized by formation upon cell substrate contact and localization at the substrate-attached part of the cell, contain an F-actin-rich core surrounded by a ring structure containing proteins such as vinculin and talin, and have a diameter of 0.5 mm.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
sarcolemma The outer membrane of a muscle cell, consisting of the plasma membrane, a covering basement membrane (about 100 nm thick and sometimes common to more than one fiber), and the associated loose network of collagen fibers.
terminal web An actin-rich cytoskeletal network located beneath the microvilli of the apical plasma membrane of polarized epithelial cells. In addition to actin filaments, the terminal web may contain actin-binding proteins, myosin motor proteins, and intermediate filaments. The terminal web can function as a contractile structure that influences the spatial distribution of microvilli as well as the development and morphogenesis of tissues containing polarized epithelial cells.
zonula adherens A cell-cell adherens junction which forms a continuous belt near the apex of epithelial cells.

5 GO annotations of molecular function

Name Definition
actin filament binding Binding to an actin filament, also known as F-actin, a helical filamentous polymer of globular G-actin subunits.
alpha-catenin binding Binding to catenin complex alpha subunit.
dystroglycan binding Binding to dystroglycan, a glycoprotein found in non-muscle tissues as well as in muscle tissues, often in association with dystrophin. The native dystroglycan cleaved into two non-covalently associated subunits, alpha (N-terminal) and beta (C-terminal).
structural molecule activity The action of a molecule that contributes to the structural integrity of a complex or its assembly within or outside a cell.
ubiquitin protein ligase binding Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.

12 GO annotations of biological process

Name Definition
adherens junction assembly The aggregation, arrangement and bonding together of a set of components to form an adherens junction. An adherens junction is a cell-cell junction composed of the epithelial cadherin-catenin complex at which the cytoplasmic face of the plasma membrane is attached to actin filaments.
apical junction assembly The formation of an apical junction, a functional unit located near the cell apex at the points of contact between epithelial cells composed of the tight junction, the zonula adherens junction and the desmosomes, by the aggregation, arrangement and bonding together of its constituents.
axon extension Long distance growth of a single axon process involved in cellular development.
cell adhesion The attachment of a cell, either to another cell or to an underlying substrate such as the extracellular matrix, via cell adhesion molecules.
epithelial cell-cell adhesion The attachment of an epithelial cell to another epithelial cell via adhesion molecules.
lamellipodium assembly Formation of a lamellipodium, a thin sheetlike extension of the surface of a migrating cell.
morphogenesis of an epithelium The process in which the anatomical structures of epithelia are generated and organized. An epithelium consists of closely packed cells arranged in one or more layers, that covers the outer surfaces of the body or lines any internal cavity or tube.
protein localization to cell surface A process in which a protein is transported to, or maintained in, a location within the external part of the cell wall and/or plasma membrane.
regulation of cell migration Any process that modulates the frequency, rate or extent of cell migration.
regulation of establishment of endothelial barrier Any process that modulates the frequency, rate or extent of establishment of endothelial barrier.
regulation of focal adhesion assembly Any process that modulates the frequency, rate or extent of focal adhesion formation, the establishment and maturation of focal adhesions.
regulation of protein localization to adherens junction Any process that modulates the frequency, rate or extent of protein localization to adherens junction. An adherens junction is a cell-cell junction composed of the epithelial cadherin-catenin complex at which the cytoplasmic face of the plasma membrane is attached to actin filaments.

16 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3MHM6 CTNNA1 Catenin alpha-1 Bos taurus (Bovine) SS
P30997 CTNNA2 Catenin alpha-2 Gallus gallus (Chicken) SS
P12003 VCL Vinculin Gallus gallus (Chicken) EV
P35220 alpha-Cat Catenin alpha Drosophila melanogaster (Fruit fly) SS
P18206 VCL Vinculin Homo sapiens (Human) SS
P26232 CTNNA2 Catenin alpha-2 Homo sapiens (Human) SS
P35221 CTNNA1 Catenin alpha-1 Homo sapiens (Human) EV
Q9UI47 CTNNA3 Catenin alpha-3 Homo sapiens (Human) SS
P26231 Ctnna1 Catenin alpha-1 Mus musculus (Mouse) EV
Q61301 Ctnna2 Catenin alpha-2 Mus musculus (Mouse) EV
Q64727 Vcl Vinculin Mus musculus (Mouse) SS
Q65CL1 Ctnna3 Catenin alpha-3 Mus musculus (Mouse) EV
P85972 Vcl Vinculin Rattus norvegicus (Rat) SS
P90947 hmp-1 Alpha-catenin-like protein hmp-1 Caenorhabditis elegans SS
A4IGI7 ctnna2 Catenin alpha-2 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
B7ZC77 Ctnna2 Catenin alpha-2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MPVFHTRTIE SILEPVAQQI SHLVIMHEEG EVDGKAIPDL TAPVAAVQAA VSNLVRVGKE
70 80 90 100 110 120
TVQTTEDQIL KRDMPPAFIK VENACTKLVQ AAQMLQSDPY SVPARDYLID GSRGILSGTS
130 140 150 160 170 180
DLLLTFDEAE VRKIIRVCKG ILEYLTVAEV VETMEDLVTY TKNLGPGMTK MAKMIDERQQ
190 200 210 220 230 240
ELTHQEHRVM LVNSMNTVKE LLPVLISAMK IFVTTKNSKN QGIEEALKNR NFTVEKMSAE
250 260 270 280 290 300
INEIIRVLQL TSWDEDAWAS KKDTEAMKRA LASIDSKLNQ AKGWLRDPTA SPGDAGEQAI
310 320 330 340 350 360
RQILDEAGKV GELCAGKERR EILGTCKMLG QMTDQVADLR ARGQGASPVA MQKAQQVSQG
370 380 390 400 410 420
LDVLTAKVEN AARKLEAMTN SKQSIAKKID AAQNWLADPN GGPEGEEQIR GALAEARKIA
430 440 450 460 470 480
ELCDDPKERD DILRSLGEIS ALTSKLADLR RQGKGDSPEA RALAKQVATA LQNLQTKTNR
490 500 510 520 530 540
AVANSRPAKA AVHLEGKIEQ AQRWIDNPTV DDRGVGQAAI RGLVAEGHRL ANVMMGPYRQ
550 560 570 580 590 600
DLLAKCDRVD QLTAQLADLA ARGEGESPQA RALASQLQDS LKDLKARMQE AMTQEVSDVF
610 620 630 640 650 660
SDTTTPIKLL AVAATAPPDA PNREEVFDER AANFENHSGR LGATAEKAAA VGTANKSTVE
670 680 690 700 710 720
GIQASVKTAR ELTPQVVSAA RILLRNPGNQ AAYEHFETMK NQWIDNVEKM TGLVDEAIDT
730 740 750 760 770 780
KSLLDASEEA IKKDLDKCKV AMANIQPQML VAGATSIARR ANRILLVAKR EVENSEDPKF
790 800 810 820 830 840
REAVKAASDE LSKTISPMVM DAKAVAGNIS DPGLQKSFLD SGYRILGAVA KVREAFQPQE
850 860 870 880 890 900
PDFPPPPPDL EQLRLTDELA PPKPPLPEGE VPPPRPPPPE EKDEEFPEQK AGEVINQPMM
910 920 930 940 950 960
MAARQLHDEA RKWSSKPGNP AAKVGIGVVA EADAADAVGF PVPSDMEDDY EPELLLMPSS
970 980 990 1000 1010 1020
QPVNQPILAA AQSLHREATK WSSKGNDIIA AAKRMALLMA EMSRLVRGGS GTKRALIQCA
1030 1040 1050 1060 1070 1080
KDIAKASDEV TRLAKEVAKQ CTDKRIRTNL LQVCERIPTI STQLKILSTV KATMLGRTNI
1090 1100 1110 1120 1130
SDEESEQATE MLVHNAQNLM QSVKETVREA EAASIKIRTD AGFTLRWVRK TPWYQ