AiPD: Autoinhibited Protein Database

P26038

Gene name	MSN
Protein name	Moesin
Names	Membrane-organizing extension spike protein
Species	Homo sapiens (Human)
KEGG Pathway	hsa:4478
EC number
Protein Class	MERLIN/MOESIN/EZRIN/RADIXIN (PTHR23281)

Descriptions

MSN is a moesin protein and belongs to the ezrin-radixin-moesin (ERM) protein family, directly involved in the cytoskeleton-membrane crosslinking. Functional N-terminal FERM domain of ERM attaches to the membrane by binding specific membrane proteins while the last 34 residues of the C-terminal tail domain bind actin filaments. The autoinhibitory domain is positions at the C-terminal tail domain of ERM, where FERM domain of ERM is bound tightly via phosphotyrosine binding (PTB), pleckstrin homology (PH), and Enabled/VASP Homology 1 (EVH1) domains, thus masking the binding sites for other molecules. ERM is activated through phosphorylation at Thr558 weakening the FERM/tail binding and, unmasks the binding sites of membrane protein and actin filaments in the presence of phospholipids.

Autoinhibitory domains (AIDs)

Target domain	5-295 (FERM domain)
Relief mechanism	PTM
Assay	Structural analysis

AID

467-577 (Tail domain)

Target domain

5-295 (FERM domain)

Relief mechanism

PTM (Phosphorylation at Thr558)

Assay

Structural analysis

Accessory elements

No accessory elements

References

Pearson MA et al. (2000) "Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domain", Cell, 101, 259-70

Austermann J et al. (2008) "Characterization of the Ca2+ -regulated ezrin-S100P interaction and its role in tumor cell migration", The Journal of biological chemistry, 283, 29331-40

Liu J et al. (2014) "Conserved sequence repeats of IQGAP1 mediate binding to Ezrin", Journal of proteome research, 13, 1156-66

Autoinhibited structure

Activated structure

10 structures for P26038

Entry ID	Method	Resolution	Chain	Position	Source
1E5W	X-ray	270 A	A	2-346	PDB
1EF1	X-ray	190 A			PDB
1SGH	X-ray	350 A	A	1-297	PDB
6TXQ	X-ray	173 A	AAA	1-346	PDB
6TXS	X-ray	220 A	AAA	1-346	PDB
8CIR	X-ray	185 A	A/B	1-346	PDB
8CIS	X-ray	152 A	A	1-346	PDB
8CIT	X-ray	254 A	A/B/C	1-346	PDB
8CIU	X-ray	239 A	A	1-346	PDB
AF-P26038-F1	Predicted				AlphaFoldDB

198 variants for P26038

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
RCV001092797 RCV000412603 CA16042218 rs1057519074 VAR_078026	171	R>W	IMD50; decreased protein abundance in T cell; loss of T cell proliferation after T cell activation; does not affect immunologic synapses formation; decreased T cell migration in response to activation by chemokines; increased T cell adhesion in response to activation by integrins Combined immunodeficiency due to moesin deficiency [UniProt, ClinVar]	Yes	ClinGen ClinVar UniProt Ensembl dbSNP
CA413412570 rs1441590143 RCV001262725	273	R>W	Combined immunodeficiency due to moesin deficiency [ClinVar]	Yes	ClinGen ClinVar TOPMed dbSNP
rs1602878106 RCV000990848 CA413414648	534	D>A	Combined immunodeficiency due to moesin deficiency [ClinVar]	Yes	ClinGen ClinVar Ensembl dbSNP
RCV000412497 rs1057519075 CA16042219	553	R>*	Combined immunodeficiency due to moesin deficiency [ClinVar]	Yes	ClinGen ClinVar Ensembl dbSNP
CA413414365 rs1311559461	5	I>F		No	ClinGen gnomAD
rs759664613 CA10434438	8	R>C		No	ClinGen ExAC TOPMed gnomAD
rs200284989 CA10434439	8	R>H		No	ClinGen ExAC TOPMed gnomAD
rs1445014192 CA413414415	10	T>S		No	ClinGen TOPMed
CA413414421 rs1334204781	11	T>P		No	ClinGen gnomAD
CA413414466 rs1280234284	17	E>A		No	ClinGen gnomAD
rs774220844 CA10434442	21	Q>H		No	ClinGen ExAC gnomAD
rs1482330736 CA413414497	22	P>T		No	ClinGen gnomAD
rs1208693016 CA413414507	23	N>S		No	ClinGen TOPMed gnomAD
rs760735957 CA413414518	25	T>A		No	ClinGen ExAC gnomAD
rs760735957 CA10434445	25	T>S		No	ClinGen ExAC gnomAD
CA10434448 rs759935225	31	D>G		No	ClinGen ExAC gnomAD
CA10434447 rs776735098	31	D>N		No	ClinGen ExAC gnomAD
rs1251973806 CA642290854	35	K>P		No	ClinGen gnomAD
rs1466800155 CA413414983	35	K>R		No	ClinGen gnomAD
rs1034323442 CA330544146	39	L>S		No	ClinGen Ensembl
rs868321933 CA330544147	41	E>K		No	ClinGen Ensembl
CA330544149 rs894507011	43	W>C		No	ClinGen Ensembl
CA413415114 rs1194956967	54	G>C		No	ClinGen gnomAD
rs182868005 CA10434460	57	T>I		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA413415137 rs182868005	57	T>N		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA413415170 rs1469446074	62	N>S		No	ClinGen TOPMed
CA10434473 rs765836962	69	D>N		No	ClinGen ExAC TOPMed gnomAD
rs201686694 CA10434474	70	V>L		No	ClinGen ExAC TOPMed gnomAD
rs201686694 CA413415235	70	V>M		No	ClinGen ExAC TOPMed gnomAD
rs754601355 CA10434475	71	R>Q		No	ClinGen ExAC gnomAD
CA330544299 rs943287676	71	R>W		No	ClinGen gnomAD
CA413415265 rs1460094115	74	S>R		No	ClinGen TOPMed gnomAD
CA413415272 rs1167329577	75	P>L		No	ClinGen gnomAD
rs1223741749 CA413415267	75	P>T		No	ClinGen TOPMed
rs1362076645 CA413415282	77	L>P		No	ClinGen TOPMed
rs140370180 CA10434476	81	R>C		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA10434477 rs772365732 COSM1215600	81	R>H	large_intestine [Cosmic]	No	ClinGen cosmic curated 1000Genomes ExAC TOPMed gnomAD
CA10434478 rs758203614	85	Y>C		No	ClinGen ExAC TOPMed gnomAD
rs746950857 CA10434480	100	R>C		No	ClinGen ExAC TOPMed gnomAD
rs770945316 CA10434481	100	R>H		No	ClinGen ExAC TOPMed gnomAD
rs1313851933 CA413415515	110	I>S		No	ClinGen gnomAD
CA413415528 rs1315877285	112	N>S		No	ClinGen gnomAD
CA330544301 rs982521952	118	P>L		No	ClinGen TOPMed
CA10434485 rs775857259	122	A>T		No	ClinGen ExAC TOPMed gnomAD
rs1474417388 CA413415654	131	Q>H		No	ClinGen gnomAD
rs1602862881 CA413415674	134	Y>C		No	ClinGen Ensembl
rs768117369 CA10434490	136	D>N		No	ClinGen ExAC gnomAD
rs753331094 CA10434491	138	N>S		No	ClinGen ExAC TOPMed gnomAD
CA413415738 rs1373451486	143	K>E		No	ClinGen gnomAD
CA10434495 rs758172397	144	S>C		No	ClinGen ExAC TOPMed gnomAD
rs199526068 CA10434494	144	S>P		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1221663220 CA413415748	145	G>S		No	ClinGen gnomAD
CA10434496 rs140037207	148	A>V		No	ClinGen ESP ExAC TOPMed gnomAD
CA10434498 COSM176053 rs757131925	149	G>R	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA413415802 rs1191782914	153	L>F		No	ClinGen gnomAD
rs1193092131 CA413415881	162	K>N		No	ClinGen gnomAD
CA10434514 rs763869268	169	E>G		No	ClinGen ExAC gnomAD
CA413415946 rs1455552920	171	R>Q		No	ClinGen TOPMed gnomAD
CA413415990 rs1407986048	177	E>G		No	ClinGen gnomAD
rs1418581438 CA413415987	177	E>Q		No	ClinGen gnomAD
CA330544385 rs886913201 COSM1124079	180	R>C	endometrium [Cosmic]	No	ClinGen cosmic curated TOPMed
COSM1124080 rs373340100 CA10434516	180	R>H	endometrium [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA10434517 rs199838289	182	M>V		No	ClinGen ExAC gnomAD
rs1435551048 CA413416058	185	E>G		No	ClinGen gnomAD
rs1306491560 CA413416081	189	L>M		No	ClinGen TOPMed
CA10434531 rs763589764	193	K>*		No	ClinGen ExAC
CA330544423 rs201406246	199	E>D		No	ClinGen TOPMed
CA10434533 rs761463986	201	Y>C		No	ClinGen ExAC gnomAD
CA10434535 rs750279044	206	F>L		No	ClinGen ExAC gnomAD
rs756098957 CA10434536	209	K>T		No	ClinGen ExAC gnomAD
CA413416278 rs1177725877	217	W>R		No	ClinGen TOPMed
rs753856958 CA10434538	220	V>G		No	ClinGen ExAC gnomAD
rs1473493317 CA413416297	220	V>M		No	ClinGen TOPMed
CA413416368 rs1195702879	230	Q>R		No	ClinGen TOPMed gnomAD
rs781669526 CA10434548	234	L>V		No	ClinGen ExAC gnomAD
rs1271572614 CA413416526	246	R>K		No	ClinGen Ensembl
rs11556591 CA330544499	247	N>S		No	ClinGen Ensembl
rs1257941102 CA413416546	249	S>P		No	ClinGen gnomAD
CA10434551 rs773831803	251	N>S		No	ClinGen ExAC TOPMed gnomAD
CA413416561 rs1450253316	251	N>Y		No	ClinGen TOPMed
CA413416605 rs1361606523	257	I>V		No	ClinGen gnomAD
CA330544500 rs12856307	264	A>D		No	ClinGen Ensembl
rs760611882 CA10434555	265	P>L		No	ClinGen ExAC gnomAD
rs139317006 CA10434554	265	P>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA10434566 rs375381171	268	V>I		No	ClinGen ESP ExAC TOPMed gnomAD
rs746416059 CA10434567	270	Y>C		No	ClinGen ExAC gnomAD
CA330544629 rs1036496677	270	Y>H		No	ClinGen TOPMed
CA413412568 rs1194926272	272	P>L		No	ClinGen gnomAD
rs945420266 CA330544630	272	P>S		No	ClinGen TOPMed gnomAD
CA413412571 rs867353730	273	R>P		No	ClinGen gnomAD
CA330544631 rs867353730	273	R>Q		No	ClinGen gnomAD
rs899329049 CA330544632	275	R>W		No	ClinGen TOPMed gnomAD
rs747717262 CA10434570	279	R>Q		No	ClinGen ExAC gnomAD
rs778459733 CA10434569	279	R>W		No	ClinGen ExAC gnomAD
CA10434571 COSM1124083 rs771845314	282	A>V	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC
CA330544633 rs932598480	284	C>F		No	ClinGen Ensembl
CA413412695 rs1459799221	292	M>L		No	ClinGen TOPMed
rs1379781650 CA413412703	293	R>G		No	ClinGen gnomAD
rs1466860878 CA413412711	294	R>H		No	ClinGen gnomAD
rs1167624194 CA413412718	295	R>H		No	ClinGen TOPMed gnomAD
CA413412751 rs1569468642	300	I>T		No	ClinGen Ensembl
rs1332152716 CA413412822	310	R>W		No	ClinGen gnomAD
rs770882351 CA10434574	318	M>I		No	ClinGen ExAC gnomAD
rs150023347 CA10434575	319	E>G		No	ClinGen ESP ExAC TOPMed gnomAD
CA10434577 COSM1257994 rs765146391	320	R>H	oesophagus [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1456206908 CA413412914	321	A>T		No	ClinGen TOPMed
rs1602873115 CA413412926	322	M>I		No	ClinGen Ensembl
rs1374521523 CA413412921	322	M>V		No	ClinGen gnomAD
rs866336389 CA330544734	324	E>K		No	ClinGen Ensembl
rs1342104235 CA413412984	330	R>H		No	ClinGen gnomAD
rs1218915738 CA413413004	332	M>I		No	ClinGen gnomAD
rs1159456307 CA413413029	336	E>A		No	ClinGen TOPMed
rs777528980 CA10434590	336	E>Q		No	ClinGen ExAC gnomAD
CA413413064 rs1265457461	340	I>M		No	ClinGen gnomAD
rs374474374 CA10434591	340	I>T		No	ClinGen ESP ExAC TOPMed gnomAD
rs745720734 CA10434594	342	R>Q		No	ClinGen ExAC gnomAD
CA10434593 rs776474722	342	R>W		No	ClinGen ExAC gnomAD
rs769709810 CA10434596	345	E>Q		No	ClinGen ExAC gnomAD
rs1177444733 CA413413106	346	E>D		No	ClinGen TOPMed
rs1417412293 CA413413108	347	L>V		No	ClinGen TOPMed gnomAD
CA10434598 rs200135811 COSM227664	349	E>G	lung skin [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA10434597 rs775305283	349	E>Q		No	ClinGen ExAC gnomAD
COSM1124085 rs182327269 CA330544735	350	R>S	endometrium [Cosmic]	No	ClinGen cosmic curated 1000Genomes
CA330544736 rs927028988	351	L>R		No	ClinGen Ensembl
rs764186464 CA10434599	352	K>R		No	ClinGen ExAC gnomAD
rs145356792 CA10434601	355	E>K		No	ClinGen ESP ExAC TOPMed gnomAD
CA10434602 rs767686320	361	A>T		No	ClinGen ExAC gnomAD
CA10434616 rs759526715	364	E>G		No	ClinGen ExAC TOPMed gnomAD
CA10434617 rs774396765	369	T>S		No	ClinGen ExAC gnomAD
CA10434618 rs761864712	370	R>C		No	ClinGen ExAC TOPMed gnomAD
CA10434619 rs767712102	370	R>H		No	ClinGen ExAC gnomAD
CA10434620 rs773318621	371	R>K		No	ClinGen ExAC gnomAD
CA413413294 rs1310782775	372	A>V		No	ClinGen gnomAD
rs992565377 CA330544756	373	L>M		No	ClinGen gnomAD
rs1421920149 CA413413309	375	L>F		No	ClinGen TOPMed
rs766622413 CA10434622	379	R>W		No	ClinGen ExAC TOPMed gnomAD
CA10434623 rs754305867	381	R>C		No	ClinGen ExAC gnomAD
CA10434624 COSM1124087 rs368873074	381	R>H	endometrium [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA413413354 rs1569468998	382	A>S		No	ClinGen Ensembl
rs1440273976 CA413413365	383	Q>H		No	ClinGen gnomAD
rs756735394 CA10434628	388	K>E		No	ClinGen ExAC gnomAD
rs780850640 CA330544757	388	K>N		No	ClinGen ExAC gnomAD
CA413413410 rs1353567681	390	A>D		No	ClinGen gnomAD
CA10434630 rs750077484	390	A>S		No	ClinGen ExAC gnomAD
rs1353567681 CA413413411	390	A>V		No	ClinGen gnomAD
CA413413432 rs1482943021	393	R>H		No	ClinGen TOPMed
CA10434631 rs767552861	394	Q>R		No	ClinGen ExAC TOPMed gnomAD
rs1461795282 CA413413443	395	E>Q		No	ClinGen gnomAD
CA413413468 rs1448225501	398	E>G		No	ClinGen gnomAD
CA10434637 rs748200741	408	R>G		No	ClinGen ExAC TOPMed gnomAD
CA413413532 rs1196941892	408	R>Q		No	ClinGen TOPMed gnomAD
CA10434636 rs748200741	408	R>W		No	ClinGen ExAC TOPMed gnomAD
CA413413615 rs1453282887	418	A>T		No	ClinGen gnomAD
CA10434653 rs748066778	421	M>T		No	ClinGen ExAC gnomAD
CA413413655 rs1394870501	423	E>D		No	ClinGen TOPMed
rs772096206 CA10434654	426	A>T		No	ClinGen ExAC TOPMed gnomAD
rs777701828 CA10434655	427	R>Q		No	ClinGen ExAC gnomAD
CA413413717 rs1403216287	433	M>I		No	ClinGen TOPMed
rs1412508043 CA413413720	434	A>T		No	ClinGen TOPMed
CA10434656 COSM296775 rs371555667	435	R>Q	large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA10434657 rs771213373	438	K>E		No	ClinGen ExAC TOPMed gnomAD
CA413413770 rs1240582009	441	E>Q		No	ClinGen TOPMed
rs1177254770 CA413413789	444	E>K		No	ClinGen TOPMed
rs777009790 CA413413814	447	Q>E		No	ClinGen ExAC TOPMed gnomAD
rs777009790 CA10434658	447	Q>K		No	ClinGen ExAC TOPMed gnomAD
CA413413848 rs1361532206	450	Q>E		No	ClinGen gnomAD
CA330544858 rs373156244	451	M>V		No	ClinGen ESP
CA10434669 rs764984537	459	T>I		No	ClinGen ExAC gnomAD
CA330544859 rs772017818	460	R>H		No	ClinGen Ensembl
rs1342406576 CA413413944	463	L>V		No	ClinGen gnomAD
rs758263845 CA10434671	466	A>S		No	ClinGen ExAC gnomAD
rs777771935 CA10434672	467	M>V		No	ClinGen ExAC TOPMed gnomAD
CA10434674 rs757438815	471	H>Q		No	ClinGen ExAC gnomAD
CA10434675 rs781526249	472	V>M		No	ClinGen ExAC gnomAD
rs770193594 CA10434678	491	S>N		No	ClinGen ExAC gnomAD
rs761952339 CA10434680	495	R>Q		No	ClinGen 1000Genomes ExAC gnomAD
rs1169768462 CA413414165	495	R>W		No	ClinGen gnomAD
rs1160945080 CA413414180	497	D>V		No	ClinGen gnomAD
CA413414190 rs1396108798	499	M>V		No	ClinGen TOPMed
rs1403645198 CA413414242	506	E>K		No	ClinGen TOPMed
CA413414263 rs1465848452	508	R>H		No	ClinGen gnomAD
CA413414270 rs1171114349	510	T>P		No	ClinGen gnomAD
rs1305168909 CA413414284	512	A>T		No	ClinGen TOPMed
CA10434686 rs759176072	518	V>L		No	ClinGen ExAC TOPMed gnomAD
rs1309870781 CA413414361	521	H>Q		No	ClinGen gnomAD
rs144972292 CA10434698	524	A>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
RCV000788875 CA413414604 rs774679103	527	S>*		No	ClinGen ClinVar ExAC TOPMed dbSNP gnomAD
CA413414602 rs1398275807	527	S>A		No	ClinGen gnomAD
CA10434699 rs774679103	527	S>L		No	ClinGen ExAC TOPMed gnomAD
rs772615370 CA10434701	531	N>I		No	ClinGen ExAC TOPMed gnomAD
rs772615370 CA10434702	531	N>S		No	ClinGen ExAC TOPMed gnomAD
CA10434703 rs759051413	537	K>R		No	ClinGen ExAC gnomAD
rs753213978 CA10434705	540	A>S		No	ClinGen ExAC gnomAD
rs1321890760 CA413414719	544	I>N		No	ClinGen TOPMed
CA413414754 rs1404562723	549	M>V		No	ClinGen TOPMed
CA330544889 COSM1124090 rs865998710	550	R>*	endometrium [Cosmic]	No	ClinGen cosmic curated Ensembl
CA413414826 COSM3845195 rs1417887388	560	R>C	breast [Cosmic]	No	ClinGen cosmic curated TOPMed
CA413414943 rs1488457028	576	S>F		No	ClinGen gnomAD

1 associated diseases with P26038

[MIM: 300988]: Immunodeficiency 50 (IMD50)

A primary immunodeficiency disorder characterized by onset of recurrent bacterial or varicella zoster virus infections in early childhood, profound lymphopenia, hypogammaglobulinemia, fluctuating monocytopenia and neutropenia, and a poor immune response to vaccine antigens. {ECO:0000269|PubMed:27405666}. Note=The disease is caused by variants affecting the gene represented in this entry.

Without disease ID

A primary immunodeficiency disorder characterized by onset of recurrent bacterial or varicella zoster virus infections in early childhood, profound lymphopenia, hypogammaglobulinemia, fluctuating monocytopenia and neutropenia, and a poor immune response to vaccine antigens. {ECO:0000269|PubMed:27405666}. Note=The disease is caused by variants affecting the gene represented in this entry.

10 regional properties for P26038

Type	Name	Position	InterPro Accession
domain	FERM domain	5 - 295	IPR000299
domain	Ezrin/radixin/moesin, C-terminal	502 - 577	IPR011259
domain	FERM, N-terminal	9 - 70	IPR018979
domain	FERM, C-terminal PH-like domain	210 - 299	IPR018980
conserved_site	FERM conserved site	58 - 88	IPR019747-1
conserved_site	FERM conserved site	176 - 205	IPR019747-2
domain	FERM central domain	91 - 206	IPR019748
domain	Band 4.1 domain	1 - 206	IPR019749
domain	ERM family, FERM domain C-lobe	200 - 296	IPR041789
domain	Ezrin/radixin/moesin, alpha-helical domain	337 - 456	IPR046810

Functions

		Description
EC Number
Subcellular Localization	Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasm, cytoskeleton Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side Cell projection, microvillus	Phosphorylated form is enriched in microvilli-like structures at apical membrane Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity) Localizes at the uropods of T lymphoblasts
PANTHER Family	PTHR23281	MERLIN/MOESIN/EZRIN/RADIXIN
PANTHER Subfamily	PTHR23281:SF26	MOESIN
PANTHER Protein Class	cytoskeletal protein actin or actin-binding cytoskeletal protein
PANTHER Pathway Category	No pathway information available

22 GO annotations of cellular component

Name	Definition
adherens junction	A cell-cell junction composed of the epithelial cadherin-catenin complex. The epithelial cadherins, or E-cadherins, of each interacting cell extend through the plasma membrane into the extracellular space and bind to each other. The E-cadherins bind to catenins on the cytoplasmic side of the membrane, where the E-cadherin-catenin complex binds to cytoskeletal components and regulatory and signaling molecules.
apical part of cell	The region of a polarized cell that forms a tip or is distal to a base. For example, in a polarized epithelial cell, the apical region has an exposed surface and lies opposite to the basal lamina that separates the epithelium from other tissue.
apical plasma membrane	The region of the plasma membrane located at the apical end of the cell.
basolateral plasma membrane	The region of the plasma membrane that includes the basal end and sides of the cell. Often used in reference to animal polarized epithelial membranes, where the basal membrane is the part attached to the extracellular matrix, or in plant cells, where the basal membrane is defined with respect to the zygotic axis.
blood microparticle	A phospholipid microvesicle that is derived from any of several cell types, such as platelets, blood cells, endothelial cells, or others, and contains membrane receptors as well as other proteins characteristic of the parental cell. Microparticles are heterogeneous in size, and are characterized as microvesicles free of nucleic acids.
cell periphery	The part of a cell encompassing the cell cortex, the plasma membrane, and any external encapsulating structures.
cell surface	The external part of the cell wall and/or plasma membrane.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoskeleton	A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
extracellular space	That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.
filopodium	Thin, stiff, actin-based protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal or dendritic growth cone, or a dendritic shaft.
focal adhesion	A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
microvillus	Thin cylindrical membrane-covered projections on the surface of an animal cell containing a core bundle of actin filaments. Present in especially large numbers on the absorptive surface of intestinal cells.
microvillus membrane	The portion of the plasma membrane surrounding a microvillus.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm	Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
pseudopodium	A temporary protrusion or retractile process of a cell, associated with flowing movements of the protoplasm, and serving for locomotion and feeding.
uropod	A membrane projection with related cytoskeletal components at the trailing edge of a cell in the process of migrating or being activated, found on the opposite side of the cell from the leading edge or immunological synapse, respectively.
vesicle	Any small, fluid-filled, spherical organelle enclosed by membrane.

7 GO annotations of molecular function

Name	Definition
actin binding	Binding to monomeric or multimeric forms of actin, including actin filaments.
cell adhesion molecule binding	Binding to a cell adhesion molecule.
double-stranded RNA binding	Binding to double-stranded RNA.
enzyme binding	Binding to an enzyme, a protein with catalytic activity.
protein kinase binding	Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
signaling receptor binding	Binding to one or more specific sites on a receptor molecule, a macromolecule that undergoes combination with a hormone, neurotransmitter, drug or intracellular messenger to initiate a change in cell function.
structural constituent of cytoskeleton	The action of a molecule that contributes to the structural integrity of a cytoskeletal structure.

19 GO annotations of biological process

Name	Definition
cellular response to testosterone stimulus	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a testosterone stimulus.
establishment of endothelial barrier	The establishment of a barrier between endothelial cell layers, such as those in the brain, lung or intestine, to exert specific and selective control over the passage of water and solutes, thus allowing formation and maintenance of compartments that differ in fluid and solute composition.
establishment of epithelial cell apical/basal polarity	The specification and formation of the apicobasal polarity of an epithelial cell.
gland morphogenesis	The process in which the anatomical structures of a gland are generated and organized.
immunological synapse formation	The formation of an area of close contact between a lymphocyte (T-, B-, or natural killer cell) and a target cell through the clustering of particular signaling and adhesion molecules and their associated membrane rafts on both the lymphocyte and target cell, which facilitates activation of the lymphocyte, transfer of membrane from the target cell to the lymphocyte, and in some situations killing of the target cell through release of secretory granules and/or death-pathway ligand-receptor interaction.
leukocyte cell-cell adhesion	The attachment of a leukocyte to another cell via adhesion molecules.
leukocyte migration	The movement of a leukocyte within or between different tissues and organs of the body.
membrane to membrane docking	The initial attachment of a membrane to a target membrane, mediated by proteins protruding from the two membranes. Docking requires only that the membranes come close enough for the proteins to interact and adhere.
positive regulation of early endosome to late endosome transport	Any process that activates or increases the frequency, rate or extent of early endosome to late endosome transport.
positive regulation of gene expression	Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of podosome assembly	Any process that activates or increases the rate or extent of podosome assembly.
positive regulation of protein localization to early endosome	Any process that activates or increases the frequency, rate or extent of protein localization to early endosome.
regulation of cell shape	Any process that modulates the surface configuration of a cell.
regulation of cell size	Any process that modulates the size of a cell.
regulation of lymphocyte migration	Any process that modulates the frequency, rate or extent of lymphocyte migration.
regulation of organelle assembly	Any process that modulates the frequency, rate or extent of organelle assembly.
T cell aggregation	The adhesion of one T cell to one or more other T cells via adhesion molecules.
T cell migration	The movement of a T cell within or between different tissues and organs of the body.
T cell proliferation	The expansion of a T cell population by cell division. Follows T cell activation.

21 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P31976	EZR	Ezrin	Bos taurus (Bovine)	PR
Q2HJ49	MSN	Moesin	Bos taurus (Bovine)	PR
Q32LP2	RDX	Radixin	Bos taurus (Bovine)	SS
Q9PU45	RDX	Radixin	Gallus gallus (Chicken)	PR
Q24564	Mer	Moesin/ezrin/radixin homolog 2	Drosophila melanogaster (Fruit fly)	SS
P46150	Moe	Moesin/ezrin/radixin homolog 1	Drosophila melanogaster (Fruit fly)	SS
Q3KP66	INAVA	Innate immunity activator protein	Homo sapiens (Human)	PR
P15311	EZR	Ezrin	Homo sapiens (Human)	EV
P35240	NF2	Merlin	Homo sapiens (Human)	EV
P35241	RDX	Radixin	Homo sapiens (Human)	SS
A2AD83	Frmd7	FERM domain-containing protein 7	Mus musculus (Mouse)	PR
P26043	Rdx	Radixin	Mus musculus (Mouse)	SS
P46662	Nf2	Merlin	Mus musculus (Mouse)	SS
P26040	Ezr	Ezrin	Mus musculus (Mouse)	SS
P26041	Msn	Moesin	Mus musculus (Mouse)	SS
P26044	RDX	Radixin	Sus scrofa (Pig)	SS
P26042	MSN	Moesin	Sus scrofa (Pig)	PR
Q63648	Nf2	Merlin	Rattus norvegicus (Rat)	SS
P31977	Ezr	Ezrin	Rattus norvegicus (Rat)	SS
O35763	Msn	Moesin	Rattus norvegicus (Rat)	SS
Q6Q413	nf2b	NF2, moesin-ezrin-radixin-like	Danio rerio (Zebrafish) (Brachydanio rerio)	SS

10	20	30	40	50	60
MPKTISVRVT	TMDAELEFAI	QPNTTGKQLF	DQVVKTIGLR	EVWFFGLQYQ	DTKGFSTWLK
70	80	90	100	110	120
LNKKVTAQDV	RKESPLLFKF	RAKFYPEDVS	EELIQDITQR	LFFLQVKEGI	LNDDIYCPPE
130	140	150	160	170	180
TAVLLASYAV	QSKYGDFNKE	VHKSGYLAGD	KLLPQRVLEQ	HKLNKDQWEE	RIQVWHEEHR
190	200	210	220	230	240
GMLREDAVLE	YLKIAQDLEM	YGVNYFSIKN	KKGSELWLGV	DALGLNIYEQ	NDRLTPKIGF
250	260	270	280	290	300
PWSEIRNISF	NDKKFVIKPI	DKKAPDFVFY	APRLRINKRI	LALCMGNHEL	YMRRRKPDTI
310	320	330	340	350	360
EVQQMKAQAR	EEKHQKQMER	AMLENEKKKR	EMAEKEKEKI	EREKEELMER	LKQIEEQTKK
370	380	390	400	410	420
AQQELEEQTR	RALELEQERK	RAQSEAEKLA	KERQEAEEAK	EALLQASRDQ	KKTQEQLALE
430	440	450	460	470	480
MAELTARISQ	LEMARQKKES	EAVEWQQKAQ	MVQEDLEKTR	AELKTAMSTP	HVAEPAENEQ
490	500	510	520	530	540
DEQDENGAEA	SADLRADAMA	KDRSEEERTT	EAEKNERVQK	HLKALTSELA	NARDESKKTA
550	560	570
NDMIHAENMR	LGRDKYKTLR	QIRQGNTKQR	IDEFESM