P25789

SS
Gene name
PSMA4 (HC9, PSC9)
Protein name
Proteasome subunit alpha type-4
Names
Macropain subunit C9 , Multicatalytic endopeptidase complex subunit C9 , Proteasome component C9 , Proteasome subunit L
Species
Homo sapiens (Human)
KEGG Pathway
hsa:5685
EC number
Protein Class

Descriptions

PRE9, proteasome subunit α type-3, is a subunit of the proteasome. The core particle (CP) is a cylindrical particle composed of four stacked rings of subunits, with each ring containing seven distinct subunits. The outer rings are composed of seven α subunits, the inner rings of seven β subunits, which contain the active sites. The N-terminal segments of the α3 subunit seals the central channel, which prevents substrate entry by imposing topological closure on the CP. Inhibition by the α3 subunit tail is relieved upon binding of the regulatory particle to the CP to form the proteasome holoenzyme.

Autoinhibitory domains (AIDs)

1-9 (N-terminal tail of the α3 subunit) SS

Target domain

29-216 (Central channel of proteasome)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

92 structures for P25789

Entry ID Method Resolution Chain Position Source
4R3O X-ray 260 A C/Q 2-251 PDB
4R67 X-ray 289 A C/Q/e/s 2-251 PDB
5A0Q EM 350 A C/Q 1-261 PDB
5GJQ EM 450 A D/j 1-261 PDB
5GJR EM 350 A D/j 1-261 PDB
5L4G EM 402 A C/P 1-261 PDB
5LE5 X-ray 180 A B/P 1-261 PDB
5LEX X-ray 220 A B/P 1-261 PDB
5LEY X-ray 190 A B/P 1-261 PDB
5LEZ X-ray 219 A B/P 1-261 PDB
5LF0 X-ray 241 A B/P 1-261 PDB
5LF1 X-ray 200 A B/P 1-261 PDB
5LF3 X-ray 210 A B/P 1-261 PDB
5LF4 X-ray 199 A B/P 1-261 PDB
5LF6 X-ray 207 A B/P 1-261 PDB
5LF7 X-ray 200 A B/P 1-261 PDB
5LN3 EM 680 A C 1-261 PDB
5M32 EM 380 A B/P 1-261 PDB
5T0C EM 380 A AI/BI 2-261 PDB
5T0G EM 440 A I 2-261 PDB
5T0H EM 680 A I 2-261 PDB
5T0I EM 800 A I 2-261 PDB
5T0J EM 800 A I 2-261 PDB
5VFO EM 350 A I/i 2-251 PDB
5VFP EM 420 A I/i 2-251 PDB
5VFQ EM 420 A I/i 2-251 PDB
5VFR EM 490 A I/i 2-251 PDB
5VFS EM 360 A I/i 2-251 PDB
5VFT EM 700 A I/i 2-251 PDB
5VFU EM 580 A I/i 2-251 PDB
6AVO EM 380 A O/Z 1-261 PDB
6E5B X-ray 277 A B/P 1-261 PDB
6KWY EM 272 A B/P 1-261 PDB
6MSB EM 300 A I/i 2-261 PDB
6MSD EM 320 A I/i 2-261 PDB
6MSE EM 330 A I/i 2-261 PDB
6MSG EM 350 A I/i 2-261 PDB
6MSH EM 360 A I/i 2-261 PDB
6MSJ EM 330 A I/i 2-261 PDB
6MSK EM 320 A I/i 2-261 PDB
6R70 EM 350 A B/P 2-249 PDB
6REY EM 300 A C/Q 1-261 PDB
6RGQ EM 260 A C/Q 1-261 PDB
6WJD EM 480 A I/i 2-261 PDB
6WJN EM 570 A I/i 2-251 PDB
6XMJ EM 300 A C 2-251 PDB
7AWE X-ray 229 A C/Q 2-257 PDB
7B12 X-ray 243 A C/Q 2-249 PDB
7LXV EM 340 A B/P 1-261 PDB
7NAN EM 280 A B/P 1-261 PDB
7NAO EM 290 A B/P 1-261 PDB
7NAP EM 320 A B/P 1-261 PDB
7NAQ EM 320 A B/P 1-261 PDB
7NHT EM 280 A B 1-261 PDB
7PG9 EM 370 A C/Q 1-261 PDB
7QXN EM 370 A I/i 2-261 PDB
7QXP EM 360 A I/i 2-261 PDB
7QXU EM 430 A I/i 2-261 PDB
7QXW EM 410 A I/i 2-261 PDB
7QXX EM 440 A I/i 2-261 PDB
7QY7 EM 470 A I/i 2-261 PDB
7QYA EM 480 A I/i 2-261 PDB
7QYB EM 410 A I/i 2-261 PDB
7V5G EM 447 A Q/X 1-261 PDB
7V5M EM 388 A C/Q 1-261 PDB
7W37 EM 300 A I/i 1-261 PDB
7W38 EM 310 A I/i 1-261 PDB
7W39 EM 320 A I/i 1-261 PDB
7W3A EM 350 A I/i 1-261 PDB
7W3B EM 360 A I/i 1-261 PDB
7W3C EM 340 A I/i 1-261 PDB
7W3F EM 330 A I/i 1-261 PDB
7W3G EM 320 A I/i 1-261 PDB
7W3H EM 320 A I/i 1-261 PDB
7W3I EM 350 A I/i 1-261 PDB
7W3J EM 350 A I/i 1-261 PDB
7W3K EM 360 A I/i 1-261 PDB
7W3M EM 350 A I/i 1-261 PDB
8BZL X-ray 214 A B/P 1-261 PDB
8CVR EM 270 A C/Q 1-261 PDB
8CVS EM 310 A B/P 1-261 PDB
8CVT EM 300 A I/i 1-261 PDB
8CXB EM 290 A B/P 1-261 PDB
8QYJ EM 273 A B 1-261 PDB
8QYL EM 267 A B 1-261 PDB
8QYM EM 273 A B 1-261 PDB
8QYN EM 288 A B 1-261 PDB
8QYO EM 284 A B/P 1-261 PDB
8QYS EM 389 A B/S 3-250 PDB
8QZ9 EM 295 A B 1-261 PDB
8UD9 EM 204 A C/Q 1-261 PDB
AF-P25789-F1 Predicted AlphaFoldDB

158 variants for P25789

Variant ID(s) Position Change Description Diseaes Association Provenance
TCGA novel
rs2052466027
 3 R>* Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
TOPMed
rs2052466514 7 S>F No Ensembl
rs11551783 8 R>K No Ensembl
rs11551787 10 T>A No Ensembl
rs2141372990 11 I>V No Ensembl
rs767513513 17 R>C No ExAC
TOPMed
gnomAD
rs750216737 17 R>H Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No ExAC
NCI-TCGA
gnomAD
rs1015360338 23 Y>* No TOPMed
gnomAD
rs2052472822 24 A>V No Ensembl
rs1367593457 25 M>V No TOPMed
gnomAD
rs2052473108 31 A>V No TOPMed
rs1169076506 34 C>Y No TOPMed
gnomAD
TCGA novel 36 G>R Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs11551779 39 A>P No Ensembl
TCGA novel 39 A>V Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1420857301 40 N>D No TOPMed
gnomAD
rs1425466879 40 N>S No TOPMed
gnomAD
rs766069798 42 G>S No ExAC
gnomAD
rs1337355352 46 A>T No gnomAD
rs2052474077 48 E>* No Ensembl
rs149336836 50 R>C No ESP
ExAC
gnomAD
rs748174564
COSM1608725
 50 R>H liver Variant assessed as Somatic; MODERATE impact. [Cosmic, NCI-TCGA] No NCI-TCGA Cosmic
cosmic curated
ExAC
NCI-TCGA
TOPMed
gnomAD
COSM471114 50 R>S Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs2052474555 51 N>D No Ensembl
rs2052474723 53 H>N No TOPMed
rs2052474794 53 H>R No Ensembl
rs2052474723 53 H>Y No TOPMed
rs2052474942 54 K>R No gnomAD
rs1170032266 55 L>F No gnomAD
COSM4057145 59 V>A Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs928841529 59 V>G No TOPMed
gnomAD
rs771384661 61 F>S No ExAC
gnomAD
TCGA novel 65 I>F Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs2052475623 65 I>M No gnomAD
rs1447160965 65 I>V No gnomAD
rs1465447435 67 K>Q No TOPMed
gnomAD
rs143441800 69 N>D No ESP
TOPMed
gnomAD
rs75116593 71 D>Y No Ensembl
rs766266828 72 M>T No ExAC
gnomAD
rs1283510396 73 A>V No TOPMed
gnomAD
COSM6143041 79 I>L Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1431318045 79 I>R No gnomAD
rs765401349 79 I>V No ExAC
gnomAD
rs752880946 80 T>N No ExAC
gnomAD
rs2141375094 84 N>S No Ensembl
rs777701656 87 T>I No ExAC
gnomAD
rs977355918 87 T>S No Ensembl
rs11551782 89 E>G No Ensembl
rs1358944038 89 E>K No gnomAD
rs2052509196 91 R>K No Ensembl
COSM125640 95 Q>E Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs764236523 97 Y>H No ExAC
gnomAD
rs2052524308 101 Y>N No Ensembl
COSM4057147 105 I>M Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs2052524478 106 P>L No gnomAD
rs1416311458 108 E>G No gnomAD
rs1422287523 109 Q>H No gnomAD
rs2052524816 111 V>A No TOPMed
gnomAD
rs942844210 111 V>I No TOPMed
gnomAD
TCGA novel 113 A>S Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1260790953 113 A>T No gnomAD
rs147984456
COSM965383
 113 A>V Variant assessed as Somatic; MODERATE impact. endometrium [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
1000Genomes
ESP
ExAC
NCI-TCGA
TOPMed
gnomAD
rs756597659 116 D>G No ExAC
gnomAD
COSM4924282 116 D>N Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
TCGA novel 119 Q>H Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
COSM4922476
rs2052525554
 119 Q>L Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
Ensembl
rs754199969 127 K>E No ExAC
COSM3370271 128 R>H Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs2052539037 133 S>T No Ensembl
TCGA novel 134 L>F Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs373384097 135 L>V No ESP
ExAC
TOPMed
gnomAD
rs2052539355 137 I>L No gnomAD
rs2052539447 141 K>T No TOPMed
rs2052539623 143 Y>S No Ensembl
rs1440320192 145 F>L No TOPMed
rs1368141469 149 Q>H No gnomAD
TCGA novel 155 N>K Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs770401245 157 G>R No ExAC
TOPMed
gnomAD
rs1567036961 158 G>V No Ensembl
rs1567036979 164 I>T No Ensembl
TCGA novel 164 I>V Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs780675206 168 S>N No ExAC
gnomAD
rs147172987 169 A>P No ESP
ExAC
TOPMed
gnomAD
rs147172987 169 A>T No ESP
ExAC
TOPMed
gnomAD
rs1596045218 171 A>G No Ensembl
rs775108437 177 Q>R No Ensembl
rs974805879 178 D>E No TOPMed
gnomAD
rs760873587 178 D>G No ExAC
gnomAD
rs1333065468 178 D>H No gnomAD
rs1333065468 178 D>Y No gnomAD
rs1403064565 181 E>G No TOPMed
rs766541253 182 G>R No ExAC
gnomAD
rs760080074 184 M>V Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No ExAC
NCI-TCGA
TOPMed
gnomAD
rs1567037856 185 T>N No Ensembl
rs1453795545 187 K>M No TOPMed
rs1287829812 188 S>L No gnomAD
rs765523469 191 A>G No ExAC
gnomAD
rs1393182567 191 A>S No TOPMed
gnomAD
rs765523469 191 A>V No ExAC
gnomAD
rs753045965 193 A>V No ExAC
TOPMed
gnomAD
rs1596045324 194 I>M No Ensembl
TCGA novel 194 I>S Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs756925719 194 I>V No ExAC
TOPMed
gnomAD
rs767116151 195 K>R No ExAC
gnomAD
TCGA novel 196 V>A Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs2052558732 199 K>Q No TOPMed
rs755670788 201 M>V No ExAC
TOPMed
gnomAD
rs749131777 206 L>P No gnomAD
rs754880889 209 E>G No ExAC
TOPMed
gnomAD
rs2052559146 209 E>K No TOPMed
rs1443754683 210 K>E No TOPMed
gnomAD
rs1172894550 211 V>G No gnomAD
rs2052601540 215 T>R No Ensembl
rs2052601453 215 T>S No Ensembl
rs1652927138 217 T>K No Ensembl
rs747235432 222 K>E No ExAC
gnomAD
rs2052602102 224 V>A No TOPMed
rs568137146 224 V>I No 1000Genomes
ExAC
TOPMed
gnomAD
rs1400757326 226 R>G No TOPMed
gnomAD
rs1567039803 226 R>I No Ensembl
TCGA novel 228 L>F Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
COSM4057148 229 K>E Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs2052602619 229 K>MFYT* No Ensembl
rs745919747 229 K>N No ExAC
gnomAD
rs2052602792 230 Q>K No TOPMed
rs1227378060 231 K>E No TOPMed
gnomAD
rs769729758 232 E>G No ExAC
gnomAD
rs776115821 233 V>A No ExAC
TOPMed
gnomAD
rs147521961 236 L>S No 1000Genomes
ESP
ExAC
TOPMed
gnomAD
rs147521961 236 L>W No 1000Genomes
ESP
ExAC
TOPMed
gnomAD
rs774633167 237 I>F No ExAC
gnomAD
rs774633167 237 I>L No ExAC
gnomAD
rs760336922 237 I>M No ExAC
TOPMed
gnomAD
TCGA novel 239 K>N Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
rs753323656 239 K>Q No ExAC
TOPMed
gnomAD
rs759129510 239 K>T No ExAC
TOPMed
gnomAD
rs8053 240 H>Q No 1000Genomes
ESP
ExAC
TOPMed
gnomAD
rs752744467 241 E>* No ExAC
TOPMed
rs752744467 241 E>K No ExAC
TOPMed
rs752744467 241 E>Q No ExAC
TOPMed
rs2094265586 241 E>V No TOPMed
rs1171027406 242 E>K No gnomAD
rs1596047200 244 E>A No Ensembl
rs975499139 246 K>Q No Ensembl
rs1420549538 247 A>T No gnomAD
COSM269960
rs751350183
 249 R>C Variant assessed as Somatic; MODERATE impact. large_intestine [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
ExAC
NCI-TCGA
TOPMed
gnomAD
rs754693970
COSM965384
 249 R>H Variant assessed as Somatic; MODERATE impact. endometrium [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
NCI-TCGA
TOPMed
gnomAD
rs754693970 249 R>L No TOPMed
gnomAD
rs113098925 250 E>G No Ensembl
TCGA novel 252 K>E Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs757449826 252 K>N No ExAC
gnomAD
COSM1374790 253 E>* Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA Cosmic
COSM3504311 253 E>G Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1065640 253 E>K No ExAC
gnomAD
rs2052604857 254 K>N No gnomAD
rs1596047247 256 Q>R No Ensembl
rs201574707 257 K>E No 1000Genomes
gnomAD
rs537707320 257 K>I No 1000Genomes
ExAC
gnomAD

No associated diseases with P25789

2 regional properties for P25789

Type Name Position InterPro Accession
domain Proteasome alpha-subunit, N-terminal domain 5 - 27 IPR000426
conserved_site Proteasome beta-type subunit, conserved site 35 - 82 IPR016050

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Translocated from the cytoplasm into the nucleus following interaction with AKIRIN2, which bridges the proteasome with the nuclear import receptor IPO9 (PubMed:34711951)
  • Colocalizes with TRIM5 in the cytoplasmic bodies (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

10 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extracellular exosome A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
intracellular membrane-bounded organelle Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
P-body A focus in the cytoplasm where mRNAs may become inactivated by decapping or some other mechanism. Protein and RNA localized to these foci are involved in mRNA degradation, nonsense-mediated mRNA decay (NMD), translational repression, and RNA-mediated gene silencing.
proteasome complex A large multisubunit complex which catalyzes protein degradation, found in eukaryotes, archaea and some bacteria. In eukaryotes, this complex consists of the barrel shaped proteasome core complex and one or two associated proteins or complexes that act in regulating entry into or exit from the core.
proteasome core complex A multisubunit barrel shaped endoprotease complex, which is the core of the proteasome complex.
proteasome core complex, alpha-subunit complex The proteasome core subcomplex that constitutes the two outer rings of the proteasome core complex. An example of this component is found in Mus musculus.

No GO annotations of molecular function

Name Definition
No GO annotations for molecular function

1 GO annotations of biological process

Name Definition
proteasome-mediated ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.

11 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P23638 PRE9 Proteasome subunit alpha type-3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EV
Q3ZCK9 PSMA4 Proteasome subunit alpha type-4 Bos taurus (Bovine) SS
Q9VA12 Prosalpha3T Proteasome subunit alpha type-4-like Drosophila melanogaster (Fruit fly) SS
P18053 Prosalpha3 Proteasome subunit alpha type-4 Drosophila melanogaster (Fruit fly) SS
Q9R1P0 Psma4 Proteasome subunit alpha type-4 Mus musculus (Mouse) SS
P21670 Psma4 Proteasome subunit alpha type-4 Rattus norvegicus (Rat) SS
Q5VRG3 Os06g0167600 Proteasome subunit alpha type-4-2 Oryza sativa subsp. japonica (Rice) SS
P0DKK3 PAC1A Proteasome subunit alpha type-4-1 Oryza sativa subsp. japonica (Rice) SS
Q9N599 pas-3 Proteasome subunit alpha type-4 Caenorhabditis elegans SS
F4JJE5 PAC2 Putative proteasome subunit alpha type-4-B Arabidopsis thaliana (Mouse-ear cress) SS
O81148 PAC1 Proteasome subunit alpha type-4-A Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR NIHKLLDEVF
70 80 90 100 110 120
FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA
130 140 150 160 170 180
YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK
190 200 210 220 230 240
EGEMTLKSAL ALAIKVLNKT MDVSKLSAEK VEIATLTREN GKTVIRVLKQ KEVEQLIKKH
250 260
EEEEAKAERE KKEKEQKEKD K