P22543

PR
Gene name
VPS34 (END12, PEP15, VPL7, VPT29)
Protein name
Phosphatidylinositol 3-kinase VPS34
Names
PI3-kinase VPS34, PI3K VPS34, PtdIns-3-kinase VPS34, Carboxypeptidase Y-deficient protein 15, Vacuolar protein sorting-associated protein 34, Vacuolar protein-targeting protein 29
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YLR240W
EC number
2.7.1.137: Phosphotransferases with an alcohol group as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

206-325 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

749-768 (Activation loop from InterPro)

Target domain

593-872 (Phosphatidylinositol 3-/4-kinase, catalytic domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

3 structures for P22543

Entry ID Method Resolution Chain Position Source
5DFZ X-ray 440 A C 1-875 PDB
5KC2 EM 2800 A C 1-875 PDB
AF-P22543-F1 Predicted AlphaFoldDB

3 variants for P22543

Variant ID(s) Position Change Description Diseaes Association Provenance
s12-618201 223 M>L No SGRP
s12-618563 343 K>N No SGRP
s12-619264 577 R>Q No SGRP

No associated diseases with P22543

5 regional properties for P22543

Type Name Position InterPro Accession
domain Phosphatidylinositol 3-/4-kinase, catalytic domain 593 - 872 IPR000403
domain Phosphoinositide 3-kinase, accessory (PIK) domain 293 - 535 IPR001263
domain C2 phosphatidylinositol 3-kinase-type domain 14 - 188 IPR002420
conserved_site Phosphatidylinositol 3/4-kinase, conserved site 623 - 637 IPR018936-1
conserved_site Phosphatidylinositol 3/4-kinase, conserved site 716 - 736 IPR018936-2

Functions

Description
EC Number 2.7.1.137 Phosphotransferases with an alcohol group as acceptor
Subcellular Localization
  • Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein
  • Endosome membrane ; Peripheral membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

13 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endosome A vacuole to which materials ingested by endocytosis are delivered.
endosome membrane The lipid bilayer surrounding an endosome.
fungal-type vacuole membrane The lipid bilayer surrounding a vacuole, the shape of which correlates with cell cycle phase. The membrane separates its contents from the cytoplasm of the cell. An example of this structure is found in Saccharomyces cerevisiae.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
nucleus-vacuole junction An organelle membrane contact site formed between the vacuole membrane and the outer nuclear membrane. In S. cerevisiae these contacts are mediated through direct physical interaction between Vac8p and Nvj1p.
peroxisome A small organelle enclosed by a single membrane, and found in most eukaryotic cells. Contains peroxidases and other enzymes involved in a variety of metabolic processes including free radical detoxification, lipid catabolism and biosynthesis, and hydrogen peroxide metabolism.
phagophore assembly site Punctate structures proximal to the endoplasmic reticulum which are the sites where the Atg machinery assembles upon autophagy induction.
phagophore assembly site membrane A cellular membrane associated with the phagophore assembly site.
phosphatidylinositol 3-kinase complex, class III, type I A class III phosphatidylinositol 3-kinase complex that is involved in autophagy. In budding yeast, this complex consists of Vps30p, Vps34p, Apg14p and Vps15p.
phosphatidylinositol 3-kinase complex, class III, type II A class III phosphatidylinositol 3-kinase complex that is involved in vacuolar protein sorting (VPS) via endosomes. In budding yeast, this complex consists of Vps30p, Vps34p, Vps38 and Vps15p.

4 GO annotations of molecular function

Name Definition
1-phosphatidylinositol-3-kinase activity Catalysis of the reaction: 1-phosphatidyl-1D-myo-inositol + ATP = a 1-phosphatidyl-1D-myo-inositol 3-phosphate + ADP + 2 H(+).
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
phosphatidylinositol kinase activity Catalysis of the reaction: ATP + a phosphatidylinositol = ADP + a phosphatidylinositol phosphate.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.

14 GO annotations of biological process

Name Definition
autophagosome assembly The formation of a double membrane-bounded structure, the autophagosome, that occurs when a specialized membrane sac, called the isolation membrane, starts to enclose a portion of the cytoplasm.
autophagy The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation.
autophagy of peroxisome The process in which peroxisomes are delivered to a type of vacuole and degraded in response to changing nutrient conditions.
endocytosis A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a small region of the plasma membrane to form a new membrane-bounded vesicle.
late endosome to vacuole transport The directed movement of substances from late endosomes to the vacuole. In yeast, after transport to the prevacuolar compartment, endocytic content is delivered to the late endosome and on to the vacuole. This pathway is analogous to endosome to lysosome transport.
macroautophagy The major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane-bounded autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane-bounded structure. Autophagosomes then fuse with a lysosome (or vacuole) releasing single-membrane-bounded autophagic bodies that are then degraded within the lysosome (or vacuole). Some types of macroautophagy, e.g. pexophagy, mitophagy, involve selective targeting of the targets to be degraded.
phosphatidylinositol phosphate biosynthetic process The chemical reactions and pathways resulting in the formation of phosphatidylinositol phosphate.
phosphatidylinositol-3-phosphate biosynthetic process The chemical reactions and pathways resulting in the formation of phosphatidylinositol-3-phosphate, a phosphatidylinositol monophosphate carrying the phosphate group at the 3-position.
phosphatidylinositol-mediated signaling The series of molecular signals in which a cell uses a phosphatidylinositol-mediated signaling to convert a signal into a response. Phosphatidylinositols include phosphatidylinositol (PtdIns) and its phosphorylated derivatives.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of macroautophagy Any process, such as recognition of nutrient depletion, that activates or increases the rate of macroautophagy to bring cytosolic macromolecules to the vacuole/lysosome for degradation.
positive regulation of transcription elongation by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides, catalyzed by RNA polymerase II.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of protein localization by the Cvt pathway Any process that modulates the frequency, rate or extent of protein localization by the Cvt pathway.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P39104 PIK1 Phosphatidylinositol 4-kinase PIK1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
10 20 30 40 50 60
MSLNNITFCV SQDLDVPLKV KIKSLEGHKP LLKPSQKILN PELMLIGSNV FPSSDLIVSL
70 80 90 100 110 120
QVFDKERNRN LTLPIYTPYI PFRNSRTWDY WLTLPIRIKQ LTFSSHLRII LWEYNGSKQI
130 140 150 160 170 180
PFFNLETSIF NLKDCTLKRG FESLKFRYDV IDHCEVVTDN KDQENLNKYF QGEFTRLPWL
190 200 210 220 230 240
DEITISKLRK QRENRTWPQG TFVLNLEFPM LELPVVFIER EIMNTQMNIP TLKNNPGLST
250 260 270 280 290 300
DLREPNRNDP QIKISLGDKY HSTLKFYDPD QPNNDPIEEK YRRLERASKN ANLDKQVKPD
310 320 330 340 350 360
IKKRDYLNKI INYPPGTKLT AHEKGSIWKY RYYLMNNKKA LTKLLQSTNL REESERVEVL
370 380 390 400 410 420
ELMDSWAEID IDDALELLGS TFKNLSVRSY AVNRLKKASD KELELYLLQL VEAVCFENLS
430 440 450 460 470 480
TFSDKSNSEF TIVDAVSSQK LSGDSMLLST SHANQKLLKS ISSESETSGT ESLPIVISPL
490 500 510 520 530 540
AEFLIRRALV NPRLGSFFYW YLKSESEDKP YLDQILSSFW SRLDKKSRNI LNDQVRLINV
550 560 570 580 590 600
LRECCETIKR LKDTTAKKME LLVHLLETKV RPLVKVRPIA LPLDPDVLIC DVCPETSKVF
610 620 630 640 650 660
KSSLSPLKIT FKTTLNQPYH LMFKVGDDLR QDQLVVQIIS LMNELLKNEN VDLKLTPYKI
670 680 690 700 710 720
LATGPQEGAI EFIPNDTLAS ILSKYHGILG YLKLHYPDEN ATLGVQGWVL DNFVKSCAGY
730 740 750 760 770 780
CVITYILGVG DRHLDNLLVT PDGHFFHADF GYILGQDPKP FPPLMKLPPQ IIEAFGGAES
790 800 810 820 830 840
SNYDKFRSYC FVAYSILRRN AGLILNLFEL MKTSNIPDIR IDPNGAILRV RERFNLNMSE
850 860 870
EDATVHFQNL INDSVNALLP IVIDHLHNLA QYWRT