P22180

SS
Gene name
LHA1
Protein name
Plasma membrane ATPase 1
Names
EC 7.1.2.1 , Proton pump 1
Species
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
KEGG Pathway
sly:543982
EC number
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.

Autoinhibitory domains (AIDs)

871-893 (Autoinhibitory domain I) SS

Target domain

313-541 (ATPase domain)

Relief mechanism

Partner binding

Assay

911-926 (Autoinhibitory domain II) SS

Target domain

313-541 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P22180

Entry ID Method Resolution Chain Position Source
AF-P22180-F1 Predicted AlphaFoldDB

No variants for P22180

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P22180

No associated diseases with P22180

3 regional properties for P22180

Type Name Position InterPro Accession
domain Cation-transporting P-type ATPase, N-terminal 17 - 89 IPR004014
ptm P-type ATPase, phosphorylation site 333 - 339 IPR018303
domain P-type ATPase, haloacid dehalogenase domain 313 - 641 IPR044492

Functions

Description
EC Number 7.1.2.1 Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Cell membrane; Multi-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

3 GO annotations of biological process

Name Definition
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
proton transmembrane transport The directed movement of a proton across a membrane.
regulation of intracellular pH Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.

13 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
Q43128 AHA10 ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P20649 AHA1 ATPase 1, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJB3 AHA5 ATPase 5, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2A0 AHA8 ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY32 AHA7 ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
P20431 AHA3 ATPase 3, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MAEKPEVLDA VLKETVDLEN IPIEEVFENL RCTREGLTAT AAQERLSIFG YNKLEEKKES
70 80 90 100 110 120
KFLKFLGFMW NPLSWVMEAA AIMAIALANG GGKPPDWQDF VGIITLLIIN STISFIEENN
130 140 150 160 170 180
AGNAAAALMA RLAPKAKVLR DGKWDEEDAS VLVPGDIISI KLGDIIPADA RLLEGDPLKI
190 200 210 220 230 240
DQSALTGESL PVTKGPGDGV YSGSTCKQGE IEAVVIATGV HTFFGKAAHL VDSTNQVGHF
250 260 270 280 290 300
QKVLTAIGNF CICSIAVGMI IEIIVMYPIQ HRKYRPGIDN LLVLLIGGIP IAMPTVLSVT
310 320 330 340 350 360
MAIGSHRLAQ QGAITKRMTA IEEMAGMDVL CSDKTGTLTL NKLTVDKALI EVFAKGIDAD
370 380 390 400 410 420
TVVLMAARAS RIENQDAIDT AIVGMLADPK EARAGIREIH FLPFNPTDKR TALTYLDGEG
430 440 450 460 470 480
KMHRVSKGAP EQILNLAHNK SDIERRVHTV IDKFAERGLR SLGVAYQEVP EGRKESAGGP
490 500 510 520 530 540
WQFIALLPLF DPPRHDSAET IRRALNLGVN VKMITGDQLA IGKETGRRLG MGTNMYPSSA
550 560 570 580 590 600
LLGQTKDESI AALPIDELIE KADGFAGVFP EHKYEIVKRL QARKHICGMT GDGVNDAPAL
610 620 630 640 650 660
KKADIGIAVD DATDAARSAS DIVLTEPGLS VIISAVLTSR AIFQRMKNYT IYAVSITIRI
670 680 690 700 710 720
VLGFMLLALI WKFDFPPFMV LIIAILNDGT IMTISKDRVK PSPLPDSWKL AEIFTTGVVL
730 740 750 760 770 780
GGYLAMMTVI FFWAAYKTNF FPRIFGVSTL EKTATDDFRK LASAIYLQVS TISQALIFVT
790 800 810 820 830 840
RSRSWSFVER PGLLLVFAFF VAQLVATLIA VYANWSFAAI EGIGWGWAGV IWLYNIVTYI
850 860 870 880 890 900
PLDLIKFLIR YALSGKAWDL VLEQRIAFTR KKDFGKELRE LQWAHAQRTL HGLQVPDPKI
910 920 930 940 950
FSETTNFNEL NQLAEEAKRR AEIARLRELH TLKGHVESVV KLKGLDIETI QQSYTV