P21734

PR
Gene name
UBC1 (YDR177W, YD9395.10)
Protein name
Ubiquitin-conjugating enzyme E2 1
Names
E2 ubiquitin-conjugating enzyme 1, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-protein ligase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YDR177W
EC number
2.3.2.23: Aminoacyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

4-73 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

4 structures for P21734

Entry ID Method Resolution Chain Position Source
1FXT NMR - A 2-150 PDB
1FZY X-ray 190 A A/B 2-150 PDB
1TTE NMR - A 1-215 PDB
AF-P21734-F1 Predicted AlphaFoldDB

3 variants for P21734

Variant ID(s) Position Change Description Diseaes Association Provenance
s04-817311 146 R>K No SGRP
s04-817322 150 S>G No SGRP
s04-817457 195 V>I No SGRP

No associated diseases with P21734

3 regional properties for P21734

Type Name Position InterPro Accession
domain Ubiquitin-conjugating enzyme E2 2 - 150 IPR000608
domain Ubiquitin-conjugating enzyme, C-terminal fungi 161 - 215 IPR015368
active_site Ubiquitin-conjugating enzyme, active site 76 - 92 IPR023313

Functions

Description
EC Number 2.3.2.23 Aminoacyltransferases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
proteasome binding Binding to a proteasome, a large multisubunit protein complex that catalyzes protein degradation.
ubiquitin conjugating enzyme activity Isoenergetic transfer of ubiquitin from one protein to another via the reaction X-ubiquitin + Y -> Y-ubiquitin + X, where both the X-ubiquitin and Y-ubiquitin linkages are thioester bonds between the C-terminal glycine of ubiquitin and a sulfhydryl side group of a cysteine residue.
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.

5 GO annotations of biological process

Name Definition
proteasome-mediated ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.
protein polyubiquitination Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain.
ubiquitin-dependent ERAD pathway The series of steps necessary to target endoplasmic reticulum (ER)-resident proteins for degradation by the cytoplasmic proteasome. Begins with recognition of the ER-resident protein, includes retrotranslocation (dislocation) of the protein from the ER to the cytosol, protein ubiquitination necessary for correct substrate transfer, transport of the protein to the proteasome, and ends with degradation of the protein by the cytoplasmic proteasome.
ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.
vesicle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a vesicle.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P33296 UBC6 Ubiquitin-conjugating enzyme E2 6 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P52490 UBC13 Ubiquitin-conjugating enzyme E2 13 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P61085 UBE2K Ubiquitin-conjugating enzyme E2 K Bos taurus (Bovine) PR
P52486 Ubc4 Ubiquitin-conjugating enzyme E2-22 kDa Drosophila melanogaster (Fruit fly) PR
P61086 UBE2K Ubiquitin-conjugating enzyme E2 K Homo sapiens (Human) PR
P61087 Ube2k Ubiquitin-conjugating enzyme E2 K Mus musculus (Mouse) PR
10 20 30 40 50 60
MSRAKRIMKE IQAVKDDPAA HITLEFVSES DIHHLKGTFL GPPGTPYEGG KFVVDIEVPM
70 80 90 100 110 120
EYPFKPPKMQ FDTKVYHPNI SSVTGAICLD ILKNAWSPVI TLKSALISLQ ALLQSPEPND
130 140 150 160 170 180
PQDAEVAQHY LRDRESFNKT AALWTRLYAS ETSNGQKGNV EESDLYGIDH DLIDEFESQG
190 200 210
FEKDKIVEVL RRLGVKSLDP NDNNTANRII EELLK