P20649

SS
Gene name
AHA1 (At2g18960, F19F24.16)
Protein name
ATPase 1, plasma membrane-type
Names
PM H, +-ATPase 1 , Plasma membrane-type ATPase 1 , EC 7.1.2.1 , Proton pump 1
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT2G18960
EC number
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.

Autoinhibitory domains (AIDs)

863-885 (Autoinhibitory domain I) SS

Target domain

309-537 (ATPase domain)

Relief mechanism

Partner binding

Assay

904-919 (Autoinhibitory domain II) SS

Target domain

309-537 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P20649

Entry ID Method Resolution Chain Position Source
AF-P20649-F1 Predicted AlphaFoldDB

8 variants for P20649

Variant ID(s) Position Change Description Diseaes Association Provenance
tmp_2_8221872_A_T 5 E>D No 1000Genomes
tmp_2_8221873_G_T 6 D>Y No 1000Genomes
tmp_2_8223238_A_G 35 T>A No 1000Genomes
tmp_2_8223898_G_A 196 V>I No 1000Genomes
ENSVATH13272492 425 A>S No 1000Genomes
ENSVATH14518429 677 L>M No 1000Genomes
tmp_2_8226408_G_A 751 D>N No 1000Genomes
ENSVATH05529760 895 P>Q No 1000Genomes

No associated diseases with P20649

11 regional properties for P20649

Type Name Position InterPro Accession
conserved_site Actinin-type actin-binding domain, conserved site 47 - 56 IPR001589-1
conserved_site Actinin-type actin-binding domain, conserved site 121 - 145 IPR001589-2
domain Calponin homology domain 45 - 149 IPR001715-1
domain Calponin homology domain 158 - 264 IPR001715-2
repeat Spectrin repeat 289 - 397 IPR002017-1
repeat Spectrin repeat 408 - 512 IPR002017-2
repeat Spectrin repeat 524 - 634 IPR002017-3
repeat Spectrin repeat 645 - 746 IPR002017-4
domain EF-hand domain 760 - 828 IPR002048
domain EF-hand, Ca insensitive 831 - 897 IPR014837
repeat Spectrin/alpha-actinin 409 - 747 IPR018159

Functions

Description
EC Number 7.1.2.1 Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Cell membrane ; Multi-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
plant-type vacuole A closed structure that is completely surrounded by a unit membrane, contains liquid, and retains the same shape regardless of cell cycle phase. An example of this structure is found in Arabidopsis thaliana.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
plasmodesma A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
magnesium ion binding Binding to a magnesium (Mg) ion.
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

7 GO annotations of biological process

Name Definition
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
proton transmembrane transport The directed movement of a proton across a membrane.
regulation of intracellular pH Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.
regulation of stomatal movement Any process that modulates the frequency, rate or extent of stomatal movement.
response to abscisic acid Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an abscisic acid stimulus.
response to water deprivation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a water deprivation stimulus, prolonged deprivation of water.
stomatal opening The process of opening of stomata, pores in the epidermis of leaves and stems bordered by two guard cells and serving in gas exchange.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
Q80XR2 Atp2c1 Calcium-transporting ATPase type 2C member 1 Mus musculus (Mouse) PR
A7L9Z8 Atp2c2 Calcium-transporting ATPase type 2C member 2 Mus musculus (Mouse) PR
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
P20431 AHA3 ATPase 3, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q43128 AHA10 ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY32 AHA7 ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2A0 AHA8 ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJB3 AHA5 ATPase 5, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P22180 LHA1 Plasma membrane ATPase 1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MSGLEDIKNE TVDLEKIPIE EVFQQLKCTR EGLTTQEGED RIVIFGPNKL EEKKESKILK
70 80 90 100 110 120
FLGFMWNPLS WVMEAAALMA IALANGDNRP PDWQDFVGII CLLVINSTIS FIEENNAGNA
130 140 150 160 170 180
AAALMAGLAP KTKVLRDGKW SEQEAAILVP GDIVSIKLGD IIPADARLLE GDPLKVDQSA
190 200 210 220 230 240
LTGESLPVTK HPGQEVFSGS TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL
250 260 270 280 290 300
TSIGNFCICS IAIGIAIEIV VMYPIQHRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG
310 320 330 340 350 360
SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC KGVEKDQVLL
370 380 390 400 410 420
FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF NPVDKRTALT YIDSDGNWHR
430 440 450 460 470 480
VSKGAPEQIL DLANARPDLR KKVLSCIDKY AERGLRSLAV ARQVVPEKTK ESPGGPWEFV
490 500 510 520 530 540
GLLPLFDPPR HDSAETIRRA LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSAALLGT
550 560 570 580 590 600
DKDSNIASIP VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD
610 620 630 640 650 660
IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV SITIRIVFGF
670 680 690 700 710 720
MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT PDSWKLKEIF ATGIVLGGYQ
730 740 750 760 770 780
AIMSVIFFWA AHKTDFFSDK FGVRSIRDNN DELMGAVYLQ VSIISQALIF VTRSRSWSFV
790 800 810 820 830 840
ERPGALLMIA FVIAQLVATL IAVYADWTFA KVKGIGWGWA GVIWIYSIVT YFPQDILKFA
850 860 870 880 890 900
IRYILSGKAW ASLFDNRTAF TTKKDYGIGE REAQWAQAQR TLHGLQPKED VNIFPEKGSY
910 920 930 940
RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVAKLKGLDI DTAGHHYTV