P20448
PR
Gene name |
HCA4 (DBP4, ECM24, YJL033W, J1250) |
Protein name |
ATP-dependent RNA helicase HCA4 |
Names |
DEAD box protein 4, Helicase CA4, Helicase UF1 |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YJL033W |
EC number |
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for P20448
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P20448-F1 | Predicted | AlphaFoldDB |
12 variants for P20448
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s10-383901 | 24 | E>K | No | SGRP | |
| s10-384010 | 60 | S>F | No | SGRP | |
| s10-384835 | 335 | T>I | No | SGRP | |
| s10-384927 | 366 | V>I | No | SGRP | |
| s10-385660 | 610 | S>N | No | SGRP | |
| s10-385720 | 630 | E>V | No | SGRP | |
| s10-385762 | 644 | E>A | No | SGRP | |
| s10-385898 | 689 | E>D | No | SGRP | |
| s10-385916 | 695 | E>D | No | SGRP | |
| s10-385985 | 718 | M>I | No | SGRP | |
| s10-385986 | 719 | P>S | No | SGRP | |
| s10-386035 | 735 | S>F | No | SGRP |
No associated diseases with P20448
9 regional properties for P20448
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | SH3 domain | 241 - 311 | IPR001452 |
| domain | PDZ domain | 153 - 234 | IPR001478 |
| domain | L27 domain | 23 - 82 | IPR004172-1 |
| domain | L27 domain | 86 - 139 | IPR004172-2 |
| domain | Guanylate kinase-like domain | 426 - 615 | IPR008144 |
| domain | Guanylate kinase/L-type calcium channel beta subunit | 425 - 618 | IPR008145 |
| domain | L27 domain, C-terminal | 89 - 135 | IPR014775 |
| conserved_site | Guanylate kinase, conserved site | 461 - 478 | IPR020590 |
| domain | MPP4, SH3 domain | 245 - 305 | IPR035600 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.6.4.13 | Acting on ATP; involved in cellular and subcellular movement |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| nucleolus | A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| small-subunit processome | A large ribonucleoprotein complex that is an early preribosomal complex. In S. cerevisiae, it has a size of 80S and consists of the 35S pre-rRNA, early-associating ribosomal proteins most of which are part of the small ribosomal subunit, the U3 snoRNA and associated proteins. |
7 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. |
| ATP-dependent activity, acting on RNA | Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction requires the presence of RNA, and it drives another reaction. |
| box C/D RNA binding | Binding to a box C/D small nucleolar RNA. |
| identical protein binding | Binding to an identical protein or proteins. |
| RNA helicase activity | Unwinding of an RNA helix, driven by ATP hydrolysis. |
| U3 snoRNA binding | Binding to a U3 small nucleolar RNA. |
2 GO annotations of biological process
| Name | Definition |
|---|---|
| maturation of SSU-rRNA | Any process involved in the maturation of a precursor Small SubUnit (SSU) ribosomal RNA (rRNA) molecule into a mature SSU-rRNA molecule. |
| rRNA processing | Any process involved in the conversion of a primary ribosomal RNA (rRNA) transcript into one or more mature rRNA molecules. |
9 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P10081 | TIF2 | ATP-dependent RNA helicase eIF4A | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P15424 | MSS116 | ATP-dependent RNA helicase MSS116, mitochondrial | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P53166 | MRH4 | ATP-dependent RNA helicase MRH4, mitochondrial | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q03532 | HAS1 | ATP-dependent RNA helicase HAS1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q06218 | DBP9 | ATP-dependent RNA helicase DBP9 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P32892 | DRS1 | ATP-dependent RNA helicase DRS1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P21372 | PRP5 | Pre-mRNA-processing ATP-dependent RNA helicase PRP5 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | EV |
| P45818 | ROK1 | ATP-dependent RNA helicase ROK1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q0D622 | Os07g0517000 | DEAD-box ATP-dependent RNA helicase 32 | Oryza sativa subsp japonica (Rice) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAKKNRLNTT | QRKTLRQKED | EYIENLKTKI | DEYDPKITKA | KFFKDLPISD | PTLKGLRESS |
| 70 | 80 | 90 | 100 | 110 | 120 |
| FIKLTEIQAD | SIPVSLQGHD | VLAAAKTGSG | KTLAFLVPVI | EKLYREKWTE | FDGLGALIIS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PTRELAMQIY | EVLTKIGSHT | SFSAGLVIGG | KDVKFELERI | SRINILIGTP | GRILQHLDQA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| VGLNTSNLQM | LVLDEADRCL | DMGFKKTLDA | IVSTLSPSRQ | TLLFSATQSQ | SVADLARLSL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| TDYKTVGTHD | VMDGSVNKEA | STPETLQQFY | IEVPLADKLD | ILFSFIKSHL | KCKMIVFLSS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| SKQVHFVYET | FRKMQPGISL | MHLHGRQKQR | ARTETLDKFN | RAQQVCLFAT | DVVARGIDFP |
| 370 | 380 | 390 | 400 | 410 | 420 |
| AVDWVVQVDC | PEDVDTYIHR | VGRCARYGKK | GKSLIMLTPQ | EQEAFLKRLN | ARKIEPGKLN |
| 430 | 440 | 450 | 460 | 470 | 480 |
| IKQSKKKSIK | PQLQSLLFKD | PELKYLGQKA | FISYVRSIYV | QKDKQVFKFD | ELPTEEFAYS |
| 490 | 500 | 510 | 520 | 530 | 540 |
| LGLPGAPKIK | MKGMKTIEQA | KERKNAPRQL | AFLSKANEDG | EVIEDKSKQP | RTKYDKMFER |
| 550 | 560 | 570 | 580 | 590 | 600 |
| KNQTILSEHY | LNITKAQAQE | DEDDDFISVK | RKDHEINEAE | LPALTLPTSR | RAQKKALSKK |
| 610 | 620 | 630 | 640 | 650 | 660 |
| ASLASKGNAS | KLIFDDEGEA | HPVYELEDEE | EFHKRGDAEV | QKTEFLTKES | AVMADIDNID |
| 670 | 680 | 690 | 700 | 710 | 720 |
| KQVAKEKKQE | KKRKRLEAMR | REMEAAMEEE | ISGDEEEGKT | VAYLGTGNLS | DDMSDGDMPD |
| 730 | 740 | 750 | 760 | ||
| SEGHLKKKAR | TVDYSHGHNP | SNSVDDDIIE | VEEPQTLEDL | ESLTAKLIQG |