P19627

SS
Gene name
Gnaz
Protein name
Guanine nucleotide-binding protein G
Names
z subunit alpha , G, x alpha chain , Gz-alpha
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:25740
EC number
Protein Class

Descriptions

The Gα subunits of heterotrimeric G proteins are GTP hydrolyases which, upon receptor activation, bind to GTP and regulate effector molecules. Gα-catalyzed hydrolysis of GTP to GDP releases the Gα-GDP product complex from effector and allows its sequestration by inhibitory Gβγ subunits. In Gia1-GppNHp complex, Gln204 of Gia1 interacts with nucleophilic water (Wnuc), participating directly in the Gia1-GTP ground state but must reorient to stabilize the transition state. RGS4, a member of the RGS family of G protein stimulatory factors, accelerates hydrolysis of GTP by Gia1 by destabilizing the ground-state conformation of Gln204. Insertion of Asn128 of RGS4 into the active site enhances catalysis by aiding in binding, orienting, and polarizing Wnuc in the pre-transition state complex. Asn128 of RGS4 displaces the side chain of Gln204 from its anti-catalytic position in the ground state, freeing it to participate in stabilization of the transition state.

Autoinhibitory domains (AIDs)

205-206 (Gln204) SS

Target domain

180-350 (P-loop containing nucleoside triphosphate hydrolase domain)

Relief mechanism

Partner binding

Assay

62-178 (α-H domain) SS

Target domain

185-349 (Ras-like domain)

Relief mechanism

Ligand binding, Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P19627

Entry ID Method Resolution Chain Position Source
AF-P19627-F1 Predicted AlphaFoldDB

No variants for P19627

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P19627

No associated diseases with P19627

No regional properties for P19627

Type Name Position InterPro Accession
No domain, repeats, and functional sites for P19627

Functions

Description
EC Number
Subcellular Localization
  • Membrane; Lipid-anchor
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cell body The portion of a cell bearing surface projections such as axons, dendrites, cilia, or flagella that includes the nucleus, but excludes all cell projections.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
heterotrimeric G-protein complex Any of a family of heterotrimeric GTP-binding and hydrolyzing proteins; they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. Heterotrimeric G-proteins consist of three subunits; the alpha subunit contains the guanine nucleotide binding site and possesses GTPase activity; the beta and gamma subunits are tightly associated and function as a beta-gamma heterodimer; extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G protein-coupled receptors to an effector protein.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

7 GO annotations of molecular function

Name Definition
adenylate cyclase inhibitor activity Binds to and decreases the activity adenylate cyclase.
G protein-coupled receptor binding Binding to a G protein-coupled receptor.
G protein-coupled serotonin receptor binding Binding to a metabotropic serotonin receptor.
G-protein beta/gamma-subunit complex binding Binding to a complex of G-protein beta/gamma subunits.
GTP binding Binding to GTP, guanosine triphosphate.
GTPase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.

4 GO annotations of biological process

Name Definition
adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway A G protein-coupled receptor signaling pathway in which the signal is transmitted via the inhibition of adenylyl cyclase activity and a subsequent decrease in the intracellular concentration of cyclic AMP (cAMP).
adenylate cyclase-modulating G protein-coupled receptor signaling pathway A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation or inhibition of adenylyl cyclase activity and a subsequent change in the intracellular concentration of cyclic AMP (cAMP).
G protein-coupled serotonin receptor signaling pathway The series of molecular signals generated as a consequence of a G protein-coupled serotonin receptor binding to one of its physiological ligands.
negative regulation of insulin secretion Any process that stops, prevents, or reduces the frequency, rate or extent of the regulated release of insulin.

62 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P63097 GNAI1 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P0C7Q4 GNAT3 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P04696 GNAT2 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P04695 GNAT1 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P08239 GNAO1 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P50147 GNAI2 Guanine nucleotide-binding protein G Gallus gallus (Chicken) SS
P50146 GNAI1 Guanine nucleotide-binding protein G Gallus gallus (Chicken) SS
P20353 Galphai G protein alpha i subunit Drosophila melanogaster (Fruit fly) SS
P16378 Galphao G protein alpha o subunit Drosophila melanogaster (Fruit fly) SS
P25157 cta Guanine nucleotide-binding protein subunit alpha homolog Drosophila melanogaster (Fruit fly) SS
P11488 GNAT1 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P19087 GNAT2 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P09471 GNAO1 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
Q03113 GNA12 Guanine nucleotide-binding protein subunit alpha-12 Homo sapiens (Human) SS
P04899 GNAI2 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P63096 GNAI1 Guanine nucleotide-binding protein G Homo sapiens (Human) EV
P08754 GNAI3 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
A8MTJ3 GNAT3 Guanine nucleotide-binding protein G Homo sapiens (Human) SS
Q14344 GNA13 Guanine nucleotide-binding protein subunit alpha-13 Homo sapiens (Human) SS
P19086 GNAZ Guanine nucleotide-binding protein G Homo sapiens (Human) SS
Q9DC51 Gnai3 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P27600 Gna12 Guanine nucleotide-binding protein subunit alpha-12 Mus musculus (Mouse) SS
P20612 Gnat1 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P27601 Gna13 Guanine nucleotide-binding protein subunit alpha-13 Mus musculus (Mouse) SS
Q3V3I2 Gnat3 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P50149 Gnat2 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P08752 Gnai2 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
B2RSH2 Gnai1 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P18872 Gnao1 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
O70443 Gnaz Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P93564 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum tuberosum (Potato) SS
P04897 Gnai2 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P08753 Gnai3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P29348 Gnat3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q63210 Gna12 Guanine nucleotide-binding protein subunit alpha-12 Rattus norvegicus (Rat) SS
Q6Q7Y5 Gna13 Guanine nucleotide-binding protein subunit alpha-13 Rattus norvegicus (Rat) SS
Q9JID2 Gna11 Guanine nucleotide-binding protein subunit alpha-11 Rattus norvegicus (Rat) SS
O88302 Gna15 Guanine nucleotide-binding protein subunit alpha-15 Rattus norvegicus (Rat) SS
P82471 Gnaq Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P38406 Gnal Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P10824 Gnai1 Guanine nucleotide-binding protein G subunit alpha-1 Rattus norvegicus (Rat) EV
P59215 Gnao1 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q63803 Gnas Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q0DJ33 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Oryza sativa subsp. japonica (Rice) SS
P51875 goa-1 Guanine nucleotide-binding protein G Caenorhabditis elegans SS
Q18434 odr-3 Guanine nucleotide-binding protein alpha-17 subunit Caenorhabditis elegans SS
Q9BIG4 gpa-10 Guanine nucleotide-binding protein alpha-10 subunit Caenorhabditis elegans PR
P28052 gpa-3 Guanine nucleotide-binding protein alpha-3 subunit Caenorhabditis elegans SS
Q9XTB2 gpa-13 Guanine nucleotide-binding protein alpha-13 subunit Caenorhabditis elegans SS
Q9BIG5 gpa-4 Guanine nucleotide-binding protein alpha-4 subunit Caenorhabditis elegans SS
P28051 gpa-1 Guanine nucleotide-binding protein alpha-1 subunit Caenorhabditis elegans SS
Q9N2V6 gpa-16 Guanine nucleotide-binding protein alpha-16 subunit Caenorhabditis elegans SS
P91907 gpa-15 Guanine nucleotide-binding protein alpha-15 subunit Caenorhabditis elegans SS
Q20907 gpa-8 Guanine nucleotide-binding protein alpha-8 subunit Caenorhabditis elegans PR
Q21917 gpa-7 Guanine nucleotide-binding protein alpha-7 subunit Caenorhabditis elegans SS
Q93743 gpa-6 Guanine nucleotide-binding protein alpha-6 subunit Caenorhabditis elegans SS
O76584 gpa-11 Guanine nucleotide-binding protein alpha-11 subunit Caenorhabditis elegans SS
P49084 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Glycine max (Soybean) (Glycine hispida) SS
P93163 GPA2 Guanine nucleotide-binding protein alpha-2 subunit Glycine max (Soybean) (Glycine hispida) SS
O80462 XLG1 Extra-large guanine nucleotide-binding protein 1 Arabidopsis thaliana (Mouse-ear cress) PR
P18064 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Arabidopsis thaliana (Mouse-ear cress) SS
P26981 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MGCRQSSEEK EAARRSRRID RHLRSESQRQ RREIKLLLLG TSNSGKSTIV KQMKIIHSGG
70 80 90 100 110 120
FNLEACKEYK PLIIYNAIDS LTRIIRALAA LKIDFHNPDR AYDAVQLFAL TGPAESKGEI
130 140 150 160 170 180
TPELLGVMRR LWADPGAQAC FGRSSEYHLE DNAAYYLNDL ERIAAPDYIP TVEDILRSRD
190 200 210 220 230 240
MTTGIVENKF TFKELTFKMV DVGGQRSERK KWIHCFEGVT AIIFCVELSG YDLKLYEDNQ
250 260 270 280 290 300
TSRMAESLRL FDSICNNNWF INTSLILFLN KKDLLSEKIR RIPLSVCFPE YKGQNTYEEA
310 320 330 340 350
AVYIQRQFED LNRNKETKEI YSHFTCATDT SNIQFVFDAV TDVIIQNNLK YIGLC