P19456
Gene name |
AHA2 |
Protein name |
ATPase 2, plasma membrane-type |
Names |
Proton pump 2 |
Species |
Arabidopsis thaliana (Mouse-ear cress) |
KEGG Pathway |
ath:AT4G30190 |
EC number |
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate |
Protein Class |
CALCIUM-TRANSPORTING ATPASE (PTHR42861) |
Descriptions
The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.
Autoinhibitory domains (AIDs)
Target domain |
309-537 (ATPase domain) |
Relief mechanism |
Partner binding |
Assay |
Mutagenesis experiment |
Target domain |
309-537 (ATPase domain) |
Relief mechanism |
Partner binding |
Assay |
Mutagenesis experiment |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
2 structures for P19456
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 5KSD | X-ray | 350 A | A/B | 12-844 | PDB |
| AF-P19456-F1 | Predicted | AlphaFoldDB |
5 variants for P19456
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| tmp_4_14774768_G_A | 47 | P>L | No | 1000Genomes | |
| tmp_4_14774288_C_T | 138 | G>E | No | 1000Genomes | |
| ENSVATH06802082 | 535 | S>L | No | 1000Genomes | |
| ENSVATH14314293 | 547 | A>T | No | 1000Genomes | |
| tmp_4_14771296_C_G | 821 | G>A | No | 1000Genomes |
No associated diseases with P19456
Functions
| Description | ||
|---|---|---|
| EC Number | 7.1.2.1 | Hydron translocation linked to the hydrolysis of a nucleoside triphosphate |
| Subcellular Localization |
|
|
| PANTHER Family | PTHR42861 | CALCIUM-TRANSPORTING ATPASE |
| PANTHER Subfamily | PTHR42861:SF154 | ATPASE 2, PLASMA MEMBRANE-TYPE |
| PANTHER Protein Class |
primary active transporter
transporter |
|
| PANTHER Pathway Category | No pathway information available | |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| integral component of membrane | The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| magnesium ion binding | Binding to a magnesium (Mg) ion. |
| P-type ion transporter activity | Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O = ADP + phosphate, to directly drive the transport of ions across a membrane. The reaction is characterized by the transient formation of a high-energy aspartyl-phosphoryl-enzyme intermediate. |
| P-type proton-exporting transporter activity | Enables the transfer of protons from one side of a membrane to the other according to the reaction: ATP + H2O + H+(in) -> ADP + phosphate + H+(out). These transporters use a phosphorylative mechanism, which have a phosphorylated intermediate state during the ion transport cycle. |
4 GO annotations of biological process
| Name | Definition |
|---|---|
| ion transmembrane transport | A process in which an ion is transported across a membrane. |
| proton export across plasma membrane | The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region. |
| proton transmembrane transport | The directed movement of a proton across a membrane. |
| regulation of intracellular pH | Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion. |
13 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P83970 | ha1 | Plasma membrane ATPase | Triticum aestivum (Wheat) | SS |
| Q7XPY2 | Os04g0656100 | Plasma membrane ATPase | Oryza sativa subsp. japonica (Rice) | SS |
| P20431 | AHA3 | ATPase 3, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P20649 | AHA1 | ATPase 1, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q42556 | AHA9 | ATPase 9, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q43128 | AHA10 | ATPase 10, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LV11 | AHA11 | ATPase 11, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LY32 | AHA7 | ATPase 7, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M2A0 | AHA8 | ATPase 8, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9SH76 | AHA6 | ATPase 6, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9SJB3 | AHA5 | ATPase 5, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9SU58 | AHA4 | ATPase 4, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P22180 | LHA1 | Plasma membrane ATPase 1 | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSSLEDIKNE | TVDLEKIPIE | EVFQQLKCSR | EGLTTQEGED | RIQIFGPNKL | EEKKESKLLK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| FLGFMWNPLS | WVMEMAAIMA | IALANGDGRP | PDWQDFVGII | CLLVINSTIS | FIEENNAGNA |
| 130 | 140 | 150 | 160 | 170 | 180 |
| AAALMAGLAP | KTKVLRDGKW | SEQEAAILVP | GDIVSIKLGD | IIPADARLLE | GDPLKVDQSA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LTGESLPVTK | HPGQEVFSGS | TCKQGEIEAV | VIATGVHTFF | GKAAHLVDST | NQVGHFQKVL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| TAIGNFCICS | IAIGMVIEII | VMYPIQRRKY | RDGIDNLLVL | LIGGIPIAMP | TVLSVTMAIG |
| 310 | 320 | 330 | 340 | 350 | 360 |
| SHRLSQQGAI | TKRMTAIEEM | AGMDVLCSDK | TGTLTLNKLS | VDKNLVEVFC | KGVEKDQVLL |
| 370 | 380 | 390 | 400 | 410 | 420 |
| FAAMASRVEN | QDAIDAAMVG | MLADPKEARA | GIREVHFLPF | NPVDKRTALT | YIDGSGNWHR |
| 430 | 440 | 450 | 460 | 470 | 480 |
| VSKGAPEQIL | ELAKASNDLS | KKVLSIIDKY | AERGLRSLAV | ARQVVPEKTK | ESPGAPWEFV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| GLLPLFDPPR | HDSAETIRRA | LNLGVNVKMI | TGDQLAIGKE | TGRRLGMGTN | MYPSSALLGT |
| 550 | 560 | 570 | 580 | 590 | 600 |
| HKDANLASIP | VEELIEKADG | FAGVFPEHKY | EIVKKLQERK | HIVGMTGDGV | NDAPALKKAD |
| 610 | 620 | 630 | 640 | 650 | 660 |
| IGIAVADATD | AARGASDIVL | TEPGLSVIIS | AVLTSRAIFQ | RMKNYTIYAV | SITIRIVFGF |
| 670 | 680 | 690 | 700 | 710 | 720 |
| MLIALIWEFD | FSAFMVLIIA | ILNDGTIMTI | SKDRVKPSPT | PDSWKLKEIF | ATGVVLGGYQ |
| 730 | 740 | 750 | 760 | 770 | 780 |
| AIMTVIFFWA | AHKTDFFSDT | FGVRSIRDNN | HELMGAVYLQ | VSIISQALIF | VTRSRSWSFV |
| 790 | 800 | 810 | 820 | 830 | 840 |
| ERPGALLMIA | FLIAQLIATL | IAVYANWEFA | KIRGIGWGWA | GVIWLYSIVT | YFPLDVFKFA |
| 850 | 860 | 870 | 880 | 890 | 900 |
| IRYILSGKAW | LNLFENKTAF | TMKKDYGKEE | REAQWALAQR | TLHGLQPKEA | VNIFPEKGSY |
| 910 | 920 | 930 | 940 | ||
| RELSEIAEQA | KRRAEIARLR | ELHTLKGHVE | SVVKLKGLDI | ETPSHYTV |