P14280

SS
Gene name
PME1.9
Protein name
Pectinesterase 1
Names
PE 1, Pectin methylesterase 1
Species
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
KEGG Pathway
sly:544090
EC number
3.1.1.11: Carboxylic ester hydrolases
Protein Class

Descriptions

(Annotation from UniProt)
The PMEI region may act as an autoinhibitory domain.

Autoinhibitory domains (AIDs)

46-197 (PMEI region) SS

Target domain

233-529 (Pectinesterase domain)

Relief mechanism

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P14280

Entry ID Method Resolution Chain Position Source
1XG2 X-ray 190 A A 230-546 PDB
AF-P14280-F1 Predicted AlphaFoldDB

No variants for P14280

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P14280

No associated diseases with P14280

4 regional properties for P14280

Type Name Position InterPro Accession
domain Pectinesterase, catalytic 233 - 529 IPR000070
domain Pectinesterase inhibitor domain 25 - 192 IPR006501
active_site Pectinesterase, Tyr active site 258 - 277 IPR018040
active_site Pectinesterase, Asp active site 377 - 386 IPR033131

Functions

Description
EC Number 3.1.1.11 Carboxylic ester hydrolases
Subcellular Localization
  • Secreted, cell wall
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
extracellular region The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.

3 GO annotations of molecular function

Name Definition
aspartyl esterase activity Catalysis of the hydrolysis of an ester bond by a mechanism involving a catalytically active aspartic acid residue.
pectinesterase activity Catalysis of the reaction: pectin + n H2O = n methanol + pectate.
pectinesterase inhibitor activity Binds to and stops, prevents or reduces the activity of pectinesterase.

3 GO annotations of biological process

Name Definition
cell wall modification The series of events leading to chemical and structural alterations of an existing cell wall that can result in loosening, increased extensibility or disassembly.
fruit ripening An developmental maturation process that has as participant a fruit. Ripening causes changes in one or more characteristics of a fruit (color, aroma, flavor, texture, hardness, cell wall structure) and may make it more attractive to animals and aid in seed dispersal.
pectin catabolic process The chemical reactions and pathways resulting in the breakdown of pectin, a polymer containing a backbone of alpha-1,4-linked D-galacturonic acid residues.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q1JPL7 PME18 Pectinesterase/pectinesterase inhibitor 18 Arabidopsis thaliana (Mouse-ear cress) SS
Q1PEC0 PME42 Probable pectinesterase/pectinesterase inhibitor 42 Arabidopsis thaliana (Mouse-ear cress) SS
Q84JX1 PME19 Probable pectinesterase/pectinesterase inhibitor 19 Arabidopsis thaliana (Mouse-ear cress) SS
P09607 PME2.1 Pectinesterase 2.1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
Q96576 PME3 Pectinesterase 3 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
Q43143 PMEU1 Pectinesterase/pectinesterase inhibitor U1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
Q96575 PME2.2 Pectinesterase 2.2 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MANPQQPLLI KTHKQNPIIS FKILSFVITL FVALFLVAPY QVEIKHSNLC KTAQDSQLCL
70 80 90 100 110 120
SYVSDLISNE IVTTESDGHS ILMKFLVNYV HQMNNAIPVV RKMKNQINDI RQHGALTDCL
130 140 150 160 170 180
ELLDQSVDFA SDSIAAIDKR SRSEHANAQS WLSGVLTNHV TCLDELDSFT KAMINGTNLE
190 200 210 220 230 240
ELISRAKVAL AMLASLTTQD EDVFMTVLGK MPSWVSSMDR KLMESSGKDI IANAVVAQDG
250 260 270 280 290 300
TGDYQTLAEA VAAAPDKSKT RYVIYVKRGT YKENVEVASN KMNLMIVGDG MYATTITGSL
310 320 330 340 350 360
NVVDGSTTFR SATLAAVGQG FILQDICIQN TAGPAKDQAV ALRVGADMSV INRCRIDAYQ
370 380 390 400 410 420
DTLYAHSQRQ FYRDSYVTGT VDFIFGNAAV VFQKCQLVAR KPGKYQQNMV TAQGRTDPNQ
430 440 450 460 470 480
ATGTSIQFCN IIASSDLEPV LKEFPTYLGR PWKEYSRTVV MESYLGGLIN PAGWAEWDGD
490 500 510 520 530 540
FALKTLYYGE FMNNGPGAGT SKRVKWPGYH VITDPAKAMP FTVAKLIQGG SWLRSTGVAY
VDGLYD