P13393

EV
Gene name
SPT15 (BTF1, TBP1, YER148W)
Protein name
TATA-box-binding protein
Names
TATA sequence-binding protein, TBP, TATA-binding factor, TATA-box factor, Transcription factor D, Transcription initiation factor TFIID TBP subunit
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YER148W
EC number
Protein Class
TATA-BOX BINDING PROTEIN (PTHR10126)

Descriptions

The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Autoinhibition of TBP through dimerization contributes to transcriptional repression, even at repressive subtelomeric regions. Point mutations within N-terminal region relieves autoinhibition.

Autoinhibitory domains (AIDs)

1-71 (Autoinhibitory element) EV

Target domain

64-237 (TATA-box binding domain)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

51 structures for P13393

Entry ID Method Resolution Chain Position Source
1NGM X-ray 295 A A/E/I/M 61-240 PDB
1NH2 X-ray 190 A A 61-240 PDB
1RM1 X-ray 250 A A 1-240 PDB
1TBA NMR - B 61-240 PDB
1TBP X-ray 260 A A/B 62-240 PDB
1YTB X-ray 180 A A/B 61-240 PDB
1YTF X-ray 250 A A 61-240 PDB
4B0A X-ray 197 A A 61-240 PDB
4V1N EM 780 A O 61-240 PDB
4V1O EM 970 A O 61-240 PDB
5FMF EM 600 A Q 61-240 PDB
5FYW EM 435 A O 1-240 PDB
5FZ5 EM 880 A O 1-240 PDB
5OQJ EM 470 A O 1-240 PDB
5OQM EM 580 A O 1-240 PDB
5SVA EM 1530 A j 1-240 PDB
6CNB EM 410 A R 61-240 PDB
6CNC EM 410 A R 61-240 PDB
6CND EM 480 A R 61-240 PDB
6CNF EM 450 A R 61-240 PDB
6E16 X-ray 240 A A 61-240 PDB
6E24 X-ray 300 A A 61-240 PDB
6EU0 EM 400 A Y 1-240 PDB
6F40 EM 370 A U 1-240 PDB
6F41 EM 430 A U 1-240 PDB
6F42 EM 550 A U 1-240 PDB
6F44 EM 420 A U 1-240 PDB
6GYK EM 510 A O 1-240 PDB
6GYL EM 480 A O 1-240 PDB
6GYM EM 670 A O 1-240 PDB
6TBM EM 2000 A M 1-240 PDB
7ML0 EM 300 A O 1-240 PDB
7ML1 EM 400 A O 1-240 PDB
7ML2 EM 340 A O 1-240 PDB
7ML4 EM 310 A O 1-240 PDB
7O4I EM 320 A O 1-240 PDB
7O4J EM 290 A O 1-240 PDB
7O72 EM 340 A O 1-240 PDB
7O73 EM 340 A O 1-240 PDB
7O75 EM 320 A O 1-240 PDB
7OH9 EM 300 A K 1-240 PDB
7OHA EM 290 A K 1-240 PDB
7OHB EM 340 A K 1-240 PDB
7Q5B EM 398 A Y 1-240 PDB
7Z0O EM 280 A H 1-240 PDB
7ZS9 EM 310 A O 1-240 PDB
7ZSA EM 400 A O 1-240 PDB
7ZSB EM 660 A O 1-240 PDB
8FFZ EM 380 A H 61-240 PDB
8H5B EM 403 A B 61-240 PDB
AF-P13393-F1 Predicted AlphaFoldDB

No variants for P13393

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P13393

No associated diseases with P13393

1 regional properties for P13393

Type Name Position InterPro Accession
conserved_site TATA-box binding protein, conserved site 185 - 234 IPR030491

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family PTHR10126 TATA-BOX BINDING PROTEIN
PANTHER Subfamily PTHR10126:SF42 TATA-BOX-BINDING PROTEIN
PANTHER Protein Class RNA metabolism protein
general transcription factor
PANTHER Pathway Category General transcription by RNA polymerase I
SL1 complex
Huntington disease
TBP
General transcription by RNA polymerase I
TBP
Transcription regulation by bZIP transcription factor
TBP

7 GO annotations of cellular component

Name Definition
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
transcription factor TFIIA complex A component of the transcription machinery of RNA Polymerase II. In humans, TFIIA is a heterotrimer composed of an alpha (P35), beta (P19) and gamma subunits (P12).
transcription factor TFIID complex A complex composed of TATA binding protein (TBP) and TBP associated factors (TAFs); the total mass is typically about 800 kDa. Most of the TAFs are conserved across species. In TATA-containing promoters for RNA polymerase II (Pol II), TFIID is believed to recognize at least two distinct elements, the TATA element and a downstream promoter element. TFIID is also involved in recognition of TATA-less Pol II promoters. Binding of TFIID to DNA is necessary but not sufficient for transcription initiation from most RNA polymerase II promoters.
transcription factor TFIIIB complex A transcription factor complex that is involved in regulating transcription from RNA polymerase III (Pol III) promoters. TFIIIB contains the TATA-binding protein (TBP) and two Pol III-specific proteins, B'' and BRF.
transcription regulator complex A protein complex that is capable of associating with DNA by direct binding, or via other DNA-binding proteins or complexes, and regulating transcription.

12 GO annotations of molecular function

Name Definition
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
disordered domain specific binding Binding to a disordered domain of a protein.
DNA binding, bending The activity of binding selectively and non-covalently to and distorting the original structure of DNA, typically a straight helix, into a bend, or increasing the bend if the original structure was intrinsically bent due to its sequence.
DNA-binding transcription factor binding Binding to a DNA-binding transcription factor, a protein that interacts with a specific DNA sequence (sometimes referred to as a motif) within the regulatory region of a gene to modulate transcription.
general transcription initiation factor activity A molecular function required for core promoter activity that mediates the assembly of the RNA polymerase holoenzyme at promoter DNA to form the pre-initiation complex (PIC). General transcription factors (GTFs) bind to and open promoter DNA, initiate RNA synthesis and stimulate the escape of the polymerase from the promoter. Not all subunits of the general transcription factor are necessarily present at all promoters to initiate transcription. GTFs act at each promoter, although the exact subunit composition at individual promoters may vary.
RNA polymerase I general transcription initiation factor binding Binding to an RNA polymerase I transcription factor, a protein required to initiate or regulate transcription by RNA polymerase I.
RNA polymerase II core promoter sequence-specific DNA binding Binding to a DNA sequence that is part of the core promoter of a RNA polymerase II-transcribed gene.
RNA polymerase II general transcription initiation factor activity A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase II. The general transcription factors for RNA polymerase II include TFIIB, TFIID, TFIIE, TFIIF, TFIIH and TATA-binding protein (TBP). In most species, RNA polymerase II transcribes all messenger RNAs (mRNAs), most untranslated regulatory RNAs, the majority of the snoRNAs, four of the five snRNAs (U1, U2, U4, and U5), and other small noncoding RNAs. For some small RNAs there is variability between species as to whether it is transcribed by RNA polymerase II or RNA polymerase III. However there are also rare exceptions, such as Trypanosoma brucei, where RNA polymerase I transcribes certain mRNAs in addition to its normal role in rRNA transcription.
RNA polymerase II-specific DNA-binding transcription factor binding Binding to a sequence-specific DNA binding RNA polymerase II transcription factor, any of the factors that interact selectively and non-covalently with a specific DNA sequence in order to modulate transcription.
RNA polymerase III general transcription initiation factor activity A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase III. Factors required for RNA polymerase III transcription initiation include TFIIIA, TFIIIB and TFIIIC. RNA polymerase III transcribes genes encoding short RNAs, including tRNAs, 5S rRNA, U6 snRNA, the short ncRNA component of RNases P, the mitochondrial RNA processing (MRP) RNA, the signal recognition particle SRP RNA, and in higher eukaryotes a number of micro and other small RNAs, though there is some variability across species as to whether a given small noncoding RNA is transcribed by RNA polymerase II or RNA polymerase III.
RNA polymerase III transcription regulatory region sequence-specific DNA binding Binding to a DNA region that controls the transcription of a gene by RNA polymerase III. Binding may occur as a sequence specific interaction or as an interaction observed only once a factor has been recruited to the DNA by other factors.
TFIIA-class transcription factor complex binding Binding to a general RNA polymerase II transcription factor belonging to the TFIIA complex, one of the complexes involved in formation of the preinitiation complex (PIC) by RNA polymerase II and defined as a basal or general transcription factor.

9 GO annotations of biological process

Name Definition
DNA-templated transcription initiation The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place.
negative regulation of DNA-templated transcription Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription.
nucleolar large rRNA transcription by RNA polymerase I The synthesis of the large ribosomal RNA (rRNA) transcript which encodes several rRNAs, e.g. in mammals 28S, 18S and 5.8S, from a nuclear DNA template transcribed by RNA polymerase I.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
regulation of transcription by RNA polymerase III Any process that modulates the frequency, rate or extent of transcription mediated by RNA ploymerase III.
RNA polymerase I preinitiation complex assembly The aggregation, arrangement and bonding together of proteins on promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription from an RNA polymerase I promoter.
RNA polymerase II preinitiation complex assembly The aggregation, arrangement and bonding together of proteins on an RNA polymerase II promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription by RNA polymerase.
RNA polymerase III preinitiation complex assembly The aggregation, arrangement and bonding together of proteins on promoter DNA to form the transcriptional preinitiation complex (PIC), the formation of which is a prerequisite for transcription from an RNA polymerase III promoter.
transposon integration Any process in which a transposable element is incorporated into another DNA molecule such as a chromosome.

21 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P26356 TBP1 TATA-box-binding protein 1 Triticum aestivum (Wheat) PR
Q02879 TBP2 TATA-box-binding protein 2 Triticum aestivum (Wheat) PR
Q2HJ52 TBP TATA-box-binding protein Bos taurus (Bovine) PR
O13270 TBP TATA-box-binding protein Gallus gallus (Chicken) PR
A6H907 TBPL2 TATA box-binding protein-like 2 Pan troglodytes (Chimpanzee) PR
Q27896 Trf TBP-related factor Drosophila melanogaster (Fruit fly) PR
Q6SJ96 TBPL2 TATA box-binding protein-like 2 Homo sapiens (Human) PR
P20226 TBP TATA-box-binding protein Homo sapiens (Human) EV
P50158 TBP1 TATA-box-binding protein 1 Zea mays (Maize) PR
P50159 TBP2 TATA-box-binding protein 2 Zea mays (Maize) PR
Q6SJ95 Tbpl2 TATA box-binding protein-like 2 Mus musculus (Mouse) PR
P29037 Tbp TATA-box-binding protein Mus musculus (Mouse) PR
A6H909 Tbpl2 TATA box-binding protein-like 2 Rattus norvegicus (Rat) PR
Q8W0W4 TBP2 TATA-binding protein 2 Oryza sativa subsp japonica (Rice) PR
Q42808 TBP1 TATA-box-binding protein Glycine max (Soybean) (Glycine hispida) PR
P28147 TBP1 TATA-box-binding protein 1 Arabidopsis thaliana (Mouse-ear cress) PR
P28148 TBP2 TATA-box-binding protein 2 Arabidopsis thaliana (Mouse-ear cress) PR
Q28DH2 tbpl2 TATA box-binding protein-like 2 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
Q28GG8 tbp TATA-box-binding protein Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
Q1JPY4 tbpl2 TATA box-binding protein-like 2 Danio rerio (Zebrafish) (Brachydanio rerio) PR
Q7SXL3 tbp TATA-box-binding protein Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT
70 80 90 100 110 120
SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK
130 140 150 160 170 180
MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG
190 200 210 220 230
TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM