P12928

SS
Gene name
Pklr
Protein name
Pyruvate kinase PKLR
Names
EC 2.7.1.40 , L-PK , Pyruvate kinase isozymes L/R
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:24651
EC number
2.7.1.40: Phosphotransferases with an alcohol group as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

432-574 (C-terminal domain) SS

Target domain

86-418 (Pyruvate kinase)

Relief mechanism

PTM, Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

3 structures for P12928

Entry ID Method Resolution Chain Position Source
6ECH X-ray 219 A A/B/C/D 34-574 PDB
6ECK X-ray 236 A A/B 34-574 PDB
AF-P12928-F1 Predicted AlphaFoldDB

1 variants for P12928

Variant ID(s) Position Change Description Diseaes Association Provenance
rs198543461 486 Q>R No EVA

No associated diseases with P12928

3 regional properties for P12928

Type Name Position InterPro Accession
domain Pyruvate kinase, barrel 86 - 418 IPR015793
domain Pyruvate kinase, C-terminal 455 - 571 IPR015795
active_site Pyruvate kinase, active site 308 - 320 IPR018209

Functions

Description
EC Number 2.7.1.40 Phosphotransferases with an alcohol group as acceptor
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
kinase activity Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
magnesium ion binding Binding to a magnesium (Mg) ion.
monosaccharide binding Binding to a monosaccharide. Monosaccharides are the simplest carbohydrates; they are polyhydroxy aldehydes HnC(=O)H or polyhydroxy ketones HnC(=O)mH with three or more carbon atoms. They form the constitutional repeating units of oligo- and polysaccharides.
potassium ion binding Binding to a potassium ion (K+).
pyruvate kinase activity Catalysis of the reaction

12 GO annotations of biological process

Name Definition
cellular response to epinephrine stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an epinephrine stimulus. Epinephrine is a catecholamine that has the formula C9H13NO3; it is secreted by the adrenal medulla to act as a hormone, and released by certain neurons to act as a neurotransmitter active in the central nervous system.
cellular response to insulin stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
glycolytic process The chemical reactions and pathways resulting in the breakdown of a carbohydrate into pyruvate, with the concomitant production of a small amount of ATP and the reduction of NAD(P) to NAD(P)H. Glycolysis begins with the metabolism of a carbohydrate to generate products that can enter the pathway and ends with the production of pyruvate. Pyruvate may be converted to acetyl-coenzyme A, ethanol, lactate, or other small molecules.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
pyruvate biosynthetic process The chemical reactions and pathways resulting in the formation of pyruvate, 2-oxopropanoate.
response to ATP Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ATP (adenosine 5'-triphosphate) stimulus.
response to cAMP Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cAMP (cyclic AMP, adenosine 3',5'-cyclophosphate) stimulus.
response to glucose Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucose stimulus.
response to hypoxia Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
response to metal ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a metal ion stimulus.
response to nutrient Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nutrient stimulus.
response to other organism Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from another living organism.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P52489 PYK2 Pyruvate kinase 2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
P00549 CDC19 Pyruvate kinase 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
P11979 PKM Pyruvate kinase PKM Felis catus (Cat) (Felis silvestris catus) SS
P00548 PKM Pyruvate kinase PKM Gallus gallus (Chicken) SS
Q29536 PKLR Pyruvate kinase PKLR Canis lupus familiaris (Dog) (Canis familiaris) SS
O62619 PyK Pyruvate kinase Drosophila melanogaster (Fruit fly) SS
P14618 PKM Pyruvate kinase PKM Homo sapiens (Human) EV
P30613 PKLR Pyruvate kinase PKLR Homo sapiens (Human) SS
P52480 Pkm Pyruvate kinase PKM Mus musculus (Mouse) SS
P53657 Pklr Pyruvate kinase PKLR Mus musculus (Mouse) SS
P11980 Pkm Pyruvate kinase PKM Rattus norvegicus (Rat) SS
Q9LIK0 PKP1 Plastidial pyruvate kinase 1, chloroplastic Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MSVQENTLPQ QLWPWIFRSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ
70 80 90 100 110 120
LPAAMADTFL EHLCLLDIDS QPVAARSTSI IATIGPASRS VDRLKEMIKA GMNIARLNFS
130 140 150 160 170 180
HGSHEYHAES IANIREATES FATSPLSYRP VAIALDTKGP EIRTGVLQGG PESEVEIVKG
190 200 210 220 230 240
SQVLVTVDPK FQTRGDAKTV WVDYHNITRV VAVGGRIYID DGLISLVVQK IGPEGLVTEV
250 260 270 280 290 300
EHGGILGSRK GVNLPNTEVD LPGLSEQDLL DLRFGVQHNV DIIFASFVRK ASDVLAVRDA
310 320 330 340 350 360
LGPEGQNIKI ISKIENHEGV KKFDEILEVS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC
370 380 390 400 410 420
NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD CIMLSGETAK GSFPVEAVMM
430 440 450 460 470 480
QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EASFKCCAAA IIVLTKTGRS
490 500 510 520 530 540
AQLLSQYRPR AAVIAVTRSA QAARQVHLSR GVFPLLYREP PEAIWADDVD RRVQFGIESG
550 560 570
KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LSVS