P11979
SS
Gene name |
PKM (PKM2) |
Protein name |
Pyruvate kinase PKM |
Names |
EC 2.7.1.40 , Pyruvate kinase muscle isozyme , Threonine-protein kinase PKM2 , EC 2.7.11.1 , Tyrosine-protein kinase PKM2 , EC 2.7.10.2 |
Species |
Felis catus (Cat) (Felis silvestris catus) |
KEGG Pathway |
|
EC number |
2.7.1.40: Phosphotransferases with an alcohol group as acceptor |
Protein Class |
|
Descriptions
Pyruvate kinase M2 (PKM2) plays a vital role in glycolysis, and it catalyzes the conversion of phosphoenolpyruvate to pyruvate with the production of ATP in the final reaction of glycolysis. PKM2 provides an in vivo growth advantage in cancer cells. Pyruvate Kinase isozymes type PKM1, PKL, and PKR exist in unstable and high-activity tetramer forms, whereas PKM2 is found in both a highly active tetramer form and a low-activity dimer form. The switch between dimer and tetramer is allosterically modulated by the binding of ligands such as amino acids and metabolic intermediates to the regulatory C-terminal domain. Post-translational modifications at specific residues relieve this inhibition, allowing metabolic flux.
Autoinhibitory domains (AIDs)
Target domain |
43-375 (Pyruvate kinase) |
Relief mechanism |
PTM, Partner binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
2 structures for P11979
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 1PKM | X-ray | 260 A | A | 2-531 | PDB |
| AF-P11979-F1 | Predicted | AlphaFoldDB |
No variants for P11979
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P11979 | |||||
No associated diseases with P11979
13 regional properties for P11979
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Receptor L-domain | 47 - 157 | IPR000494-1 |
| domain | Receptor L-domain | 346 - 455 | IPR000494-2 |
| domain | Protein kinase domain | 979 - 1254 | IPR000719 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 980 - 1245 | IPR001245 |
| conserved_site | Tyrosine-protein kinase, receptor class II, conserved site | 1139 - 1147 | IPR002011 |
| domain | Fibronectin type III | 483 - 603 | IPR003961-1 |
| domain | Fibronectin type III | 605 - 798 | IPR003961-2 |
| domain | Fibronectin type III | 816 - 913 | IPR003961-3 |
| domain | Furin-like cysteine-rich domain | 174 - 329 | IPR006211 |
| repeat | Furin-like repeat | 225 - 275 | IPR006212 |
| active_site | Tyrosine-protein kinase, active site | 1111 - 1123 | IPR008266 |
| binding_site | Protein kinase, ATP binding site | 985 - 1013 | IPR017441 |
| domain | Tyrosine-protein kinase, catalytic domain | 979 - 1246 | IPR020635 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.1.40 | Phosphotransferases with an alcohol group as acceptor |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| rough endoplasmic reticulum | The rough (or granular) endoplasmic reticulum (ER) has ribosomes adhering to the outer surface; the ribosomes are the site of translation of the mRNA for those proteins which are either to be retained within the cisternae (ER-resident proteins), the proteins of the lysosomes, or the proteins destined for export from the cell. Glycoproteins undergo their initial glycosylation within the cisternae. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| magnesium ion binding | Binding to a magnesium (Mg) ion. |
| mRNA binding | Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns. |
| potassium ion binding | Binding to a potassium ion (K+). |
| protein tyrosine kinase activity | Catalysis of the reaction |
| pyruvate kinase activity | Catalysis of the reaction |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| cellular response to insulin stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms. |
| glycolytic process | The chemical reactions and pathways resulting in the breakdown of a carbohydrate into pyruvate, with the concomitant production of a small amount of ATP and the reduction of NAD(P) to NAD(P)H. Glycolysis begins with the metabolism of a carbohydrate to generate products that can enter the pathway and ends with the production of pyruvate. Pyruvate may be converted to acetyl-coenzyme A, ethanol, lactate, or other small molecules. |
| phosphorylation | The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. |
| positive regulation of cytoplasmic translation | Any process that activates or increases the frequency, rate or extent of cytoplasmic translation. |
| positive regulation of sprouting angiogenesis | Any process that activates or increases the frequency, rate or extent of sprouting angiogenesis. |
12 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P52489 | PYK2 | Pyruvate kinase 2 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| P00549 | CDC19 | Pyruvate kinase 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| P00548 | PKM | Pyruvate kinase PKM | Gallus gallus (Chicken) | SS |
| Q29536 | PKLR | Pyruvate kinase PKLR | Canis lupus familiaris (Dog) (Canis familiaris) | SS |
| O62619 | PyK | Pyruvate kinase | Drosophila melanogaster (Fruit fly) | SS |
| P14618 | PKM | Pyruvate kinase PKM | Homo sapiens (Human) | EV |
| P30613 | PKLR | Pyruvate kinase PKLR | Homo sapiens (Human) | SS |
| P53657 | Pklr | Pyruvate kinase PKLR | Mus musculus (Mouse) | SS |
| P52480 | Pkm | Pyruvate kinase PKM | Mus musculus (Mouse) | SS |
| P12928 | Pklr | Pyruvate kinase PKLR | Rattus norvegicus (Rat) | SS |
| P11980 | Pkm | Pyruvate kinase PKM | Rattus norvegicus (Rat) | SS |
| Q9LIK0 | PKP1 | Plastidial pyruvate kinase 1, chloroplastic | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSKPHSDVGT | AFIQTQQLHA | AMADTFLEHM | CRLDIDSPPI | TARNTGIICT | IGPASRSVEI |
| 70 | 80 | 90 | 100 | 110 | 120 |
| LKEMIKSGMN | VARLNFSHGT | HEYHAETIKN | VRAATESFAS | DPIRYRPVAV | ALDTKGPEIR |
| 130 | 140 | 150 | 160 | 170 | 180 |
| TGLIKGSGTA | EVELKKGATL | KITLDNAYME | KCDENVLWLD | YKNICKVVEV | GSKVYVDDGL |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ISLLVKEKGA | DFLVTEVENG | GSLGSKKGVN | LPGAAVDLPA | VSEKDIQDLK | FGVEQDVDMV |
| 250 | 260 | 270 | 280 | 290 | 300 |
| FASFIRKASD | VHEVRKVLGE | KGKNIKIISK | IENHEGVRRF | DEILEASDGI | MVARGDLGIE |
| 310 | 320 | 330 | 340 | 350 | 360 |
| IPAEKVFLAQ | KMMIGRCNRA | GKPVICATQM | LESMIKKPRP | TRAEGSDVAN | AVLDGADCIM |
| 370 | 380 | 390 | 400 | 410 | 420 |
| LSGETAKGDY | PLEAVRMQHL | IAREAEAAMF | HRKLFEELVR | GSSHSTDLME | AMAMGSVEAS |
| 430 | 440 | 450 | 460 | 470 | 480 |
| YKCLAAALIV | LTESGRSAHQ | VARYRPRAPI | IAVTRNHQTA | RQAHLYRGIF | PVVCKDPVQE |
| 490 | 500 | 510 | 520 | 530 | |
| AWAEDVDLRV | NLAMNVGKAR | GFFKHGDVVI | VLTGWRPGSG | FTNTMRVVPV | P |