AiPD: Autoinhibited Protein Database

P11142

Gene name	HSPA8
Protein name	Heat shock cognate 71 kDa protein
Names	Heat shock 70 kDa protein 8, Lipopolysaccharide-associated protein 1, LAP-1, LPS-associated protein 1
Species	Homo sapiens (Human)
KEGG Pathway	hsa:3312
EC number	3.6.4.10: Acting on ATP; involved in cellular and subcellular movement
Protein Class	HEAT SHOCK PROTEIN 70KDA (PTHR19375)

Descriptions

HSPA8 (or hsc70) is a heat shock cognate protein involved in many cellular processes. During recovery from stress, HSPA8, initially accumulating in the nucleoplasm for folding/refolding of proteins, transiently concentrates in nucleoli for nucleolar morphology and function restoration. The inhibitory region is positioned at one of the three domains of HSPA8 protein, which is the N-terminal ATPase domain, specifically in residues 263-287 of domain IIB. Under normal growth conditions, the constitutive nucleolar targeting function that is provided by residues 225-262 is diminished by the autoinhibitory element located in residues 263-287. When cells recover from stress, the autoinhibitory element is inactivated, and the constitutive nucleolar targeting function restores.

Autoinhibitory domains (AIDs)

Target domain	225-262 (Nucleolar targeting region)
Relief mechanism	Others
Assay	Deletion assay, Mutagenesis experiment

AID

263-287 (Autoinhibitory region)

Target domain

225-262 (Nucleolar targeting region)

Relief mechanism

Others (Cell stress recovery)

Assay

Deletion assay, Mutagenesis experiment

Accessory elements

No accessory elements

References

Bański P et al. (2010) "Nucleolar targeting of the chaperone hsc70 is regulated by stress, cell signaling, and a composite targeting signal which is controlled by autoinhibition", The Journal of biological chemistry, 285, 21858-67

Autoinhibited structure

Activated structure

39 structures for P11142

Entry ID	Method	Resolution	Chain	Position	Source
3AGY	X-ray	185 A	C/D/F	639-646	PDB
3AGZ	X-ray	251 A	C/D/E/F	639-646	PDB
3ESK	X-ray	205 A	B	635-646	PDB
3FZF	X-ray	220 A	A	4-381	PDB
3FZH	X-ray	200 A	A	4-381	PDB
3FZK	X-ray	210 A	A	4-381	PDB
3FZL	X-ray	220 A	A	4-381	PDB
3FZM	X-ray	230 A	A	4-381	PDB
3LDQ	X-ray	190 A	A	4-381	PDB
3M3Z	X-ray	210 A	A	4-381	PDB
4H5N	X-ray	186 A	A/B	2-384	PDB
4H5R	X-ray	164 A	A/B	2-384	PDB
4H5T	X-ray	190 A	A	2-384	PDB
4H5V	X-ray	175 A	A	2-384	PDB
4H5W	X-ray	194 A	A/B	2-384	PDB
4HWI	X-ray	227 A	A	5-381	PDB
4KBQ	X-ray	291 A	C/D	541-646	PDB
5AQF	X-ray	188 A	A/C	1-381	PDB
5AQG	X-ray	224 A	A/C/E	1-381	PDB
5AQH	X-ray	200 A	A	1-381	PDB
5AQI	X-ray	198 A	A/C	1-381	PDB
5AQJ	X-ray	196 A	A/C/E	1-381	PDB
5AQK	X-ray	209 A	A	1-381	PDB
5AQL	X-ray	169 A	A/C	1-381	PDB
5AQM	X-ray	163 A	A/C	1-381	PDB
5AQN	X-ray	245 A	A/C/E	1-381	PDB
5AQO	X-ray	212 A	A/C/E	1-381	PDB
5AQP	X-ray	208 A	A/C/E	1-381	PDB
5AQQ	X-ray	272 A	A/C/E	1-381	PDB
5AQR	X-ray	191 A	A/C/E	1-381	PDB
5AQS	X-ray	200 A	A/C	1-381	PDB
5AQT	X-ray	190 A	A	1-381	PDB
5AQU	X-ray	192 A	A	1-381	PDB
5AQV	X-ray	175 A	A	1-381	PDB
6B1I	X-ray	230 A	A/B	5-381	PDB
6B1M	X-ray	190 A	A/B	5-381	PDB
6B1N	X-ray	180 A	A/B	5-381	PDB
6ZYJ	X-ray	185 A	A/B	5-384	PDB
AF-P11142-F1	Predicted				AlphaFoldDB

211 variants for P11142

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1470313419 CA383061178	3	K>R		No	ClinGen TOPMed
CA6332870 rs749485084	5	P>L		No	ClinGen ExAC gnomAD
CA383061165 rs1449602133	5	P>S		No	ClinGen TOPMed gnomAD
CA6332869 rs778022948	6	A>S		No	ClinGen ExAC TOPMed gnomAD
CA383061058 rs1427745924	22	Q>*		No	ClinGen gnomAD
rs1565369691 CA383061008	28	I>M		No	ClinGen Ensembl
rs11551602 CA229970012 VAR_049619	32	D>Y		No	ClinGen UniProt Ensembl dbSNP
rs950960741 CA229969991	41	Y>*		No	ClinGen Ensembl
rs765012279 CA6332860	41	Y>C		No	ClinGen ExAC gnomAD
CA229970001 rs75739900	41	Y>H		No	ClinGen Ensembl
rs201477863 CA229969960	45	T>M		No	ClinGen 1000Genomes gnomAD
CA229969963 rs201477863	45	T>R		No	ClinGen 1000Genomes gnomAD
CA383060890 rs1166201725	46	D>E		No	ClinGen gnomAD
rs770980780 CA6332854	47	T>A		No	ClinGen ExAC gnomAD
CA383060858 rs376156706	51	I>M		No	ClinGen ESP ExAC TOPMed gnomAD
rs1252070613 CA383060792 COSM202734	61	M>T	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed
rs764381465 CA6332812	73	L>V		No	ClinGen ExAC TOPMed gnomAD
CA383060600 rs1328280148	87	M>R		No	ClinGen gnomAD
CA383060559 rs775043697	92	F>L		No	ClinGen ExAC TOPMed gnomAD
rs1366901063 CA383060550	93	M>I		No	ClinGen gnomAD
CA383060553 rs1445257474	93	M>T		No	ClinGen gnomAD
CA383060556 rs1283020583 COSM924154	93	M>V	endometrium [Cosmic]	No	ClinGen cosmic curated gnomAD
CA383060539 rs1591439268	95	V>G		No	ClinGen Ensembl
rs777228127 CA6332804	96	N>S		No	ClinGen ExAC TOPMed gnomAD
rs1388306788 CA383060520	98	A>S		No	ClinGen gnomAD
rs1591439236 CA383060483	103	V>G		No	ClinGen Ensembl
CA6332798 rs766369333	104	Q>R		No	ClinGen ExAC TOPMed gnomAD
rs200601870 CA6332797	106	E>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA229969389 rs748008431	107	Y>C		No	ClinGen Ensembl
CA383060448 rs1464641326	108	K>N		No	ClinGen gnomAD
CA229969372 rs781044959	109	G>E		No	ClinGen Ensembl
CA383060434 rs760971468	110	E>D		No	ClinGen ExAC TOPMed gnomAD
rs1289131268 CA383060415	113	S>N		No	ClinGen gnomAD
rs11551608 CA229969354	116	P>T		No	ClinGen gnomAD
CA229969348 rs879004891	123	V>L		No	ClinGen Ensembl
rs1286131382 CA383060309	128	K>R		No	ClinGen TOPMed
CA383060296 rs1591439159	130	I>V		No	ClinGen Ensembl
CA6332784 rs780873363	132	E>G		No	ClinGen ExAC gnomAD
CA6332785 rs747769427	132	E>Q		No	ClinGen ExAC gnomAD
rs1281904116 CA383060254	136	G>A		No	ClinGen TOPMed
CA383060233 rs1591438530	138	T>P		No	ClinGen Ensembl
rs774366889 CA6332737	138	T>S		No	ClinGen ExAC gnomAD
rs141156763 CA229968728	140	T>N		No	ClinGen 1000Genomes ExAC TOPMed
rs141156763 CA6332735	140	T>S		No	ClinGen 1000Genomes ExAC TOPMed
rs569651128 CA6332733	141	N>S		No	ClinGen ExAC gnomAD
CA6332732 rs755052827	142	A>V		No	ClinGen ExAC gnomAD
CA229968693 rs11551603	157	A>V		No	ClinGen Ensembl
rs11551606 CA229968692	158	T>I		No	ClinGen Ensembl
CA383060057 rs1565368756	165	A>P		No	ClinGen Ensembl
CA229968654 rs866713011	182	A>S		No	ClinGen Ensembl
rs199619043 CA6332698	189	V>G		No	ClinGen ExAC gnomAD
rs775427523 CA6332694	191	A>G		No	ClinGen ExAC gnomAD
rs769624839 CA6332695	191	A>T		No	ClinGen ExAC gnomAD
rs775427523 CA6332693	191	A>V		No	ClinGen ExAC gnomAD
CA6332690 rs779126635	194	N>K		No	ClinGen ExAC TOPMed gnomAD
rs778283472 CA6332687	209	I>V		No	ClinGen ExAC TOPMed gnomAD
CA383059678 rs1431250178	220	K>Q		No	ClinGen gnomAD
rs11551599 CA6332684	222	T>A		No	ClinGen ExAC gnomAD
CA383059659 rs1402476075	223	A>S		No	ClinGen gnomAD
rs1427790009 CA383059567	236	R>*		No	ClinGen gnomAD
rs1184917258 CA383059566	236	R>Q		No	ClinGen gnomAD
rs536669943 CA6332681	239	N>S		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs765204439 CA229968387	243	A>S		No	ClinGen Ensembl
rs1228883118 CA383059458	251	K>E		No	ClinGen gnomAD
CA6332674 rs759639896	253	I>V		No	ClinGen ExAC gnomAD
rs1591438086 CA383059421	255	E>D		No	ClinGen Ensembl
CA383059380 rs777998627	261	R>S		No	ClinGen ExAC gnomAD
CA383059379 rs777998627	261	R>S		No	ClinGen ExAC gnomAD
COSM42681 CA229968335 rs1003257093	262	R>C	central_nervous_system [Cosmic]	No	ClinGen cosmic curated TOPMed
rs770238029 CA6332669	263	L>F		No	ClinGen ExAC TOPMed gnomAD
rs748585744 CA6332668	264	R>C		No	ClinGen ExAC gnomAD
CA383059357 rs1277369023	266	A>T		No	ClinGen TOPMed
CA229968288 rs11551604	269	R>C		No	ClinGen Ensembl
CA383059333 rs1440328569 COSM1559337	269	R>H	liver central_nervous_system [Cosmic]	No	ClinGen cosmic curated TOPMed
CA229968274 rs11551605	272	R>C		No	ClinGen Ensembl
rs1239556225 CA383059314	272	R>H		No	ClinGen TOPMed
rs751121248 CA6332665	274	L>V		No	ClinGen ExAC gnomAD
rs750129947 CA6332662	282	I>T		No	ClinGen ExAC gnomAD
CA383059232 rs148846437	284	I>M		No	ClinGen ESP ExAC TOPMed gnomAD
rs1446357941 CA383059208	288	Y>C		No	ClinGen gnomAD
CA383059210 rs1216243283	288	Y>H		No	ClinGen gnomAD
CA6332657 rs532778326	291	I>V		No	ClinGen ExAC TOPMed gnomAD
CA383059183 rs1313762341	292	D>N		No	ClinGen gnomAD
CA383059169 rs1366918784	293	F>L		No	ClinGen gnomAD
CA6332654 rs763133730	295	T>I		No	ClinGen ExAC TOPMed gnomAD
CA6332653 rs773536032	296	S>F		No	ClinGen ExAC gnomAD
rs778115074 CA6332650	305	L>V		No	ClinGen ExAC gnomAD
CA6332648 rs747578888	307	A>V		No	ClinGen ExAC gnomAD
rs1565368268 CA383059053	311	R>H		No	ClinGen Ensembl
COSM1195742 CA229968107 rs1000333769	317	V>I	lung [Cosmic]	No	ClinGen cosmic curated TOPMed
CA383059001 rs1223624505	319	K>R		No	ClinGen gnomAD
CA383058932 rs1215490797	329	S>L		No	ClinGen gnomAD
CA6332640 rs373235233	330	Q>L		No	ClinGen ESP ExAC gnomAD
CA6332638 rs766425028	331	I>M		No	ClinGen ExAC TOPMed gnomAD
rs752672546 CA6332639	331	I>V		No	ClinGen ExAC TOPMed gnomAD
rs1302623127 CA383058918	332	H>D		No	ClinGen gnomAD
rs763116637 CA6332637 COSM1704727	334	I>T	skin [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA383058902 rs1422888266	334	I>V		No	ClinGen TOPMed gnomAD
rs1591437770 CA383058854	342	R>S		No	ClinGen Ensembl
CA6332634 rs762028517	349	L>F		No	ClinGen ExAC gnomAD
rs1410331792 CA383058799	350	L>F		No	ClinGen TOPMed
CA6332631 rs747492962	353	F>Y		No	ClinGen ExAC TOPMed gnomAD
rs771663386 CA383058766	354	F>L		No	ClinGen ExAC TOPMed gnomAD
rs1251414344 CA383058760	355	N>S		No	ClinGen gnomAD
CA6332626 rs756920138	360	N>S		No	ClinGen ExAC gnomAD
CA6332540 rs746781077	374	A>V		No	ClinGen ExAC gnomAD
CA383058543 rs1250487224	385	S>Y		No	ClinGen gnomAD
rs745866854 CA383058533	386	E>D		No	ClinGen ExAC TOPMed gnomAD
CA383058517 rs1565367621	389	Q>E		No	ClinGen Ensembl
rs1395408526 CA383058435	398	P>S		No	ClinGen gnomAD
CA6332532 rs755304850	399	L>F		No	ClinGen ExAC gnomAD
CA383058371 rs1245759796 COSM123666	403	I>V	upper_aerodigestive_tract [Cosmic]	No	ClinGen cosmic curated TOPMed
rs372928718 CA6332530	409	V>I		No	ClinGen ESP ExAC TOPMed gnomAD
CA383058257 rs1480411360	411	T>S		No	ClinGen gnomAD
CA383058250 rs1397356715	412	V>I		No	ClinGen TOPMed
CA383058202 rs1184086544	415	K>N		No	ClinGen gnomAD
CA383058171 rs1591437035	418	T>P		No	ClinGen Ensembl
rs1591437024 CA383058155	419	T>P		No	ClinGen Ensembl
rs1263548986 CA383058091	424	Q>K		No	ClinGen gnomAD
rs763663403 CA6332524	427	T>I		No	ClinGen ExAC gnomAD
rs1555074367 CA383058012	429	T>S		No	ClinGen Ensembl
CA6332521 rs772031159	434	N>S		No	ClinGen ExAC gnomAD
CA383057864 rs1226429373	440	I>V		No	ClinGen TOPMed
CA229967216 rs1029079461	441	Q>*		No	ClinGen TOPMed
CA383057491 rs1426678244	442	V>A		No	ClinGen gnomAD
rs1163128822 CA383057417	453	N>D		No	ClinGen gnomAD
rs11551598 VAR_049620 CA229966880	459	F>L		No	ClinGen UniProt Ensembl dbSNP
rs746441724 COSM924146 CA6332478	462	T>A	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA229966875 rs925044940	464	I>L		No	ClinGen TOPMed gnomAD
rs1290423049 CA383057330	465	P>L		No	ClinGen gnomAD
rs1042085144 CA229966870	466	P>A		No	ClinGen TOPMed
rs773253340 CA6332477	466	P>L		No	ClinGen ExAC gnomAD
CA383057286 rs1555074255	473	Q>R		No	ClinGen Ensembl
rs1302645687 CA383057195	486	L>F		No	ClinGen gnomAD
CA6332469 rs749707173	487	N>S		No	ClinGen ExAC TOPMed gnomAD
rs1451018519 CA383057103	499	N>S		No	ClinGen TOPMed
CA6332465 rs780908558	500	K>R		No	ClinGen ExAC gnomAD
CA6332462 rs766069802	504	T>S		No	ClinGen ExAC gnomAD
rs1219667932 CA383056965	509	R>C		No	ClinGen gnomAD
CA383056947 rs1255945488	511	S>N		No	ClinGen gnomAD
rs1355011382 CA383056919	515	I>V		No	ClinGen gnomAD
CA383056903 rs1180559000	517	R>C		No	ClinGen TOPMed gnomAD
rs771746646 CA6332423	517	R>H		No	ClinGen ExAC TOPMed gnomAD
CA6332424 rs771746646	517	R>L		No	ClinGen ExAC TOPMed gnomAD
CA229966466 rs909420393	518	M>V		No	ClinGen TOPMed
CA6332421 rs778743630	519	V>I		No	ClinGen ExAC gnomAD
rs753733140 CA6332419	524	K>E		No	ClinGen ExAC gnomAD
CA383056843 rs1314984351	526	K>Q		No	ClinGen gnomAD
CA383056831 rs1355626720	527	A>G		No	ClinGen TOPMed
rs1324030336 CA383056811	530	E>K		No	ClinGen TOPMed
rs766437819 CA6332415	533	R>K		No	ClinGen ExAC gnomAD
rs763204793 CA6332414	534	D>A		No	ClinGen ExAC gnomAD
rs1417379667 CA383056783	534	D>N		No	ClinGen gnomAD
rs1359549189 CA383056711	544	S>T		No	ClinGen TOPMed
CA6332408 rs769269163	545	Y>C		No	ClinGen ExAC
rs761149194 CA6332406	546	A>S		No	ClinGen ExAC gnomAD
rs775032110 CA6332404	547	F>L		No	ClinGen ExAC gnomAD
CA6332402 rs568778183	549	M>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs745475251 CA6332401	550	K>R		No	ClinGen ExAC TOPMed gnomAD
rs745475251 CA383056668	550	K>T		No	ClinGen ExAC TOPMed gnomAD
rs1334087304 CA383056660	551	A>G		No	ClinGen TOPMed
rs778459169 CA6332398	552	T>A		No	ClinGen ExAC gnomAD
CA383056651 rs1307984893	553	V>A		No	ClinGen TOPMed gnomAD
CA383056652 rs770645829	553	V>F		No	ClinGen ExAC gnomAD
CA383056649 rs1307984893	553	V>G		No	ClinGen TOPMed gnomAD
CA6332397 rs770645829	553	V>I		No	ClinGen ExAC gnomAD
rs749093478 CA6332395	559	Q>E		No	ClinGen ExAC TOPMed gnomAD
rs749093478 CA383056610	559	Q>K		No	ClinGen ExAC TOPMed gnomAD
rs1468625294 CA383056607	559	Q>R		No	ClinGen TOPMed
rs777603150 CA6332394	562	I>V		No	ClinGen ExAC gnomAD
CA6332392 rs199882006	563	N>K		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs756041393 CA6332393	563	N>S		No	ClinGen ExAC TOPMed gnomAD
CA229966371 rs765561454	564	D>N		No	ClinGen TOPMed gnomAD
CA383056567 rs1463886962	565	E>G		No	ClinGen gnomAD
CA383056554 rs1420825439	567	K>Q		No	ClinGen TOPMed
rs754246861 CA6332386	574	C>S		No	ClinGen ExAC gnomAD
CA6332385 rs764589322	578	I>V		No	ClinGen ExAC gnomAD
rs1317857757 CA383056463	579	N>S		No	ClinGen TOPMed
rs1480815795 CA383056421	584	N>K		No	ClinGen gnomAD
CA383056419 rs1591435739	585	Q>K		No	ClinGen Ensembl
rs963847272 CA229965801	586	T>S		No	ClinGen TOPMed
rs1385383759 CA383056392	587	A>T		No	ClinGen gnomAD
CA383056352 rs1339299808	592	F>S		No	ClinGen gnomAD
CA383056344 rs1320337416	593	E>G		No	ClinGen gnomAD
rs41487746 CA383056333	594	H>Q		No	ClinGen TOPMed
rs776255814 CA6332306	595	Q>K		No	ClinGen ExAC TOPMed gnomAD
CA229965729 rs775363477	596	Q>K		No	ClinGen Ensembl
CA383056322 rs1591434728	596	Q>R		No	ClinGen Ensembl
rs1322018211 CA383056317	597	K>E		No	ClinGen TOPMed
rs760638348 CA6332304	598	E>K		No	ClinGen ExAC gnomAD
CA383056291 rs1162494106	600	E>D		No	ClinGen gnomAD
CA6332301 rs377681653	605	P>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs1056656311 CA229965705	607	I>V		No	ClinGen TOPMed
CA383056238 rs1487646632	608	T>S		No	ClinGen TOPMed gnomAD
rs781388678 CA6332297	610	L>R		No	ClinGen ExAC gnomAD
CA383056200 rs1228155115	614	A>T		No	ClinGen gnomAD
CA6332295 rs752022565	614	A>V		No	ClinGen ExAC TOPMed gnomAD
rs1401181468 CA383056195	615	G>R		No	ClinGen gnomAD
rs780546314 CA6332294	616	G>S		No	ClinGen ExAC gnomAD
CA383056182 rs1296437764	616	G>V		No	ClinGen gnomAD
rs1401650031 CA383056175	617	M>I		No	ClinGen TOPMed
CA6332293 rs757849635	620	G>E		No	ClinGen ExAC TOPMed gnomAD
rs1261030266 CA383056151	621	M>T		No	ClinGen gnomAD
CA383056145 rs1199609003	622	P>A		No	ClinGen TOPMed gnomAD
rs1199609003 CA383056146	622	P>T		No	ClinGen TOPMed gnomAD
CA383056129 rs1403445047	624	G>V		No	ClinGen gnomAD
CA6332290 rs764872570	628	G>S		No	ClinGen ExAC gnomAD
CA383056103 rs1477455686	629	G>R		No	ClinGen gnomAD
CA6332288 rs753546825	630	A>V		No	ClinGen ExAC gnomAD
CA229965662 rs946722582	633	S>C		No	ClinGen TOPMed
rs1219260728 CA383056052	637	S>F		No	ClinGen TOPMed

No associated diseases with P11142

2 regional properties for P11142

Type	Name	Position	InterPro Accession
domain	Kinesin motor domain	3 - 343	IPR001752
conserved_site	Kinesin motor domain, conserved site	234 - 245	IPR019821

Functions

		Description
EC Number	3.6.4.10	Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization	Cytoplasm Melanosome Nucleus, nucleolus Cell membrane Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side	Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:17289661) Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock (PubMed:1586970)
PANTHER Family	PTHR19375	HEAT SHOCK PROTEIN 70KDA
PANTHER Subfamily	PTHR19375:SF379	HEAT SHOCK COGNATE 71 KDA PROTEIN
PANTHER Protein Class	Hsp70 family chaperone
PANTHER Pathway Category	Parkinson disease Hsp70 Apoptosis signaling pathway HSP70

35 GO annotations of cellular component

Name	Definition
autophagosome	A double-membrane-bounded compartment that engulfs endogenous cellular material as well as invading microorganisms to target them to the lytic vacuole/lysosome for degradation as part of macroautophagy.
blood microparticle	A phospholipid microvesicle that is derived from any of several cell types, such as platelets, blood cells, endothelial cells, or others, and contains membrane receptors as well as other proteins characteristic of the parental cell. Microparticles are heterogeneous in size, and are characterized as microvesicles free of nucleic acids.
chaperone complex	A protein complex required for the non-covalent folding or unfolding, maturation, stabilization or assembly or disassembly of macromolecular structures. Usually active during or immediately after completion of translation. Many chaperone complexes contain heat shock proteins.
clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane	The lipid bilayer surrounding a clathrin-sculpted gamma-aminobutyric acid transport vesicle.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendrite	A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
extracellular region	The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
extracellular space	That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.
ficolin-1-rich granule lumen	Any membrane-enclosed lumen that is part of a ficolin-1-rich granule.
focal adhesion	A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
glutamatergic synapse	A synapse that uses glutamate as a neurotransmitter.
glycinergic synapse	A synapse that uses glycine as a neurotransmitter.
late endosome	A prelysosomal endocytic organelle differentiated from early endosomes by lower lumenal pH and different protein composition. Late endosomes are more spherical than early endosomes and are mostly juxtanuclear, being concentrated near the microtubule organizing center.
lumenal side of lysosomal membrane	The side (leaflet) of the lysosomal membrane that faces the lumen.
lysosomal lumen	The volume enclosed within the lysosomal membrane.
lysosomal membrane	The lipid bilayer surrounding the lysosome and separating its contents from the cell cytoplasm.
lysosome	A small lytic vacuole that has cell cycle-independent morphology found in most animal cells and that contains a variety of hydrolases, most of which have their maximal activities in the pH range 5-6. The contained enzymes display latency if properly isolated. About 40 different lysosomal hydrolases are known and lysosomes have a great variety of morphologies and functions.
melanosome	A tissue-specific, membrane-bounded cytoplasmic organelle within which melanin pigments are synthesized and stored. Melanosomes are synthesized in melanocyte cells.
membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
nucleolus	A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm	Cytoplasm situated near, or occurring around, the nucleus.
photoreceptor ribbon synapse	A ribbon synapse between a retinal photoreceptor cell (rod or cone) and a retinal bipolar cell. These contain a plate-like synaptic ribbon.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
postsynaptic cytosol	The region of the cytosol consisting of all cytosol that is part of the postsynapse.
postsynaptic specialization membrane	The membrane component of the postsynaptic specialization. This is the region of the postsynaptic membrane in which the population of neurotransmitter receptors involved in synaptic transmission are concentrated.
presynaptic cytosol	The region of the cytosol consisting of all cytosol that is part of the presynapse.
Prp19 complex	A protein complex consisting of Prp19 and associated proteins that is involved in the transition from the precatalytic spliceosome to the activated form that catalyzes step 1 of splicing, and which remains associated with the spliceosome through the second catalytic step. It is widely conserved, found in both yeast and mammals, though the exact composition varies. In S. cerevisiae, it contains Prp19p, Ntc20p, Snt309p, Isy1p, Syf2p, Cwc2p, Prp46p, Clf1p, Cef1p, and Syf1p.
ribonucleoprotein complex	A macromolecular complex that contains both RNA and protein molecules.
secretory granule lumen	The volume enclosed by the membrane of a secretory granule.
spliceosomal complex	Any of a series of ribonucleoprotein complexes that contain snRNA(s) and small nuclear ribonucleoproteins (snRNPs), and are formed sequentially during the spliceosomal splicing of one or more substrate RNAs, and which also contain the RNA substrate(s) from the initial target RNAs of splicing, the splicing intermediate RNA(s), to the final RNA products. During cis-splicing, the initial target RNA is a single, contiguous RNA transcript, whether mRNA, snoRNA, etc., and the released products are a spliced RNA and an excised intron, generally as a lariat structure. During trans-splicing, there are two initial substrate RNAs, the spliced leader RNA and a pre-mRNA.
terminal bouton	Terminal inflated portion of the axon, containing the specialized apparatus necessary to release neurotransmitters. The axon terminus is considered to be the whole region of thickening and the terminal bouton is a specialized region of it.

19 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity	Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
C3HC4-type RING finger domain binding	Binding to a C3HC4-type zinc finger domain of a protein. The C3HC4-type zinc finger is a variant of RING finger, is a cysteine-rich domain of 40 to 60 residues that coordinates two zinc ions, and has the consensus sequence: C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-C-X2-C-X(4-48)-C-X2-C, where X is any amino acid. Many proteins containing a C3HC4-type RING finger play a key role in the ubiquitination pathway.
cadherin binding	Binding to cadherin, a type I membrane protein involved in cell adhesion.
chaperone binding	Binding to a chaperone protein, a class of proteins that bind to nascent or unfolded polypeptides and ensure correct folding or transport.
clathrin-uncoating ATPase activity	Catalysis of the reaction: ATP + H2O = ADP + phosphate. Catalysis of the removal of clathrin from vesicle membranes, coupled to the hydrolysis of ATP.
enzyme binding	Binding to an enzyme, a protein with catalytic activity.
G protein-coupled receptor binding	Binding to a G protein-coupled receptor.
heat shock protein binding	Binding to a heat shock protein, a protein synthesized or activated in response to heat shock.
MHC class II protein complex binding	Binding to a class II major histocompatibility complex.
misfolded protein binding	Binding to a misfolded protein.
phosphatidylserine binding	Binding to phosphatidylserine, a class of glycophospholipids in which a phosphatidyl group is esterified to the hydroxyl group of L-serine.
protein carrier chaperone	Binding to and carrying a protein between two different cellular components by moving along with the target protein.
protein disaggregase activity	An ATP-dependent molecular chaperone activity that mediates the solubilization of ordered protein aggregates.
protein folding chaperone	Binding to a protein or a protein-containing complex to assist the protein folding process.
protein-macromolecule adaptor activity	The binding activity of a protein that brings together two or more macromolecules in contact, permitting those molecules to function in a coordinated way. The adaptor can bring together two proteins, or a protein and another macromolecule such as a lipid or a nucleic acid.
RNA binding	Binding to an RNA molecule or a portion thereof.
ubiquitin protein ligase binding	Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.
unfolded protein binding	Binding to an unfolded protein.

27 GO annotations of biological process

Name	Definition
ATP metabolic process	The chemical reactions and pathways involving ATP, adenosine triphosphate, a universally important coenzyme and enzyme regulator.
cellular response to starvation	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nourishment.
cellular response to steroid hormone stimulus	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a steroid hormone stimulus.
cellular response to unfolded protein	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an unfolded protein stimulus.
chaperone cofactor-dependent protein refolding	The process of assisting in the correct posttranslational noncovalent assembly of proteins, which is dependent on additional protein cofactors. This process occurs over one or several cycles of nucleotide hydrolysis-dependent binding and release.
chaperone-mediated autophagy	The autophagy process which begins when chaperones and co-chaperones recognize a target motif and unfold the substrate protein. The proteins are then transported to the lysosome where they are degraded.
chaperone-mediated autophagy translocation complex disassembly	The disaggregation of a chaperone-mediated autophagy translocation complex into its constituent components.
late endosomal microautophagy	The autophagy process by which cytosolic proteins targeted for degradation are tagged with a chaperone and are directly transferred into and degraded in a late endosomal compartment.
membrane organization	A process which results in the assembly, arrangement of constituent parts, or disassembly of a membrane. A membrane is a double layer of lipid molecules that encloses all cells, and, in eukaryotes, many organelles; may be a single or double lipid bilayer; also includes associated proteins.
mRNA processing	Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.
negative regulation of supramolecular fiber organization	Any process that stops, prevents or reduces the frequency, rate or extent of fibril organization.
negative regulation of transcription, DNA-templated	Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription.
positive regulation by host of viral genome replication	A process in which a host organism activates or increases the frequency, rate or extent of viral genome replication.
positive regulation of mRNA splicing, via spliceosome	Any process that activates or increases the rate or extent of mRNA splicing via a spliceosomal mechanism.
protein folding	The process of assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure.
protein refolding	The process carried out by a cell that restores the biological activity of an unfolded or misfolded protein, using helper proteins such as chaperones.
protein targeting to lysosome involved in chaperone-mediated autophagy	The targeting of a protein to the lysosome process in which an input protein binds to a chaperone and subsequently to a lysosomal receptor.
regulation of cell cycle	Any process that modulates the rate or extent of progression through the cell cycle.
regulation of postsynapse organization	Any process that modulates the physical form of a postsynapse.
regulation of protein complex stability	Any process that affects the structure and integrity of a protein complex by altering the likelihood of its assembly or disassembly.
regulation of protein import	Any process that modulates the frequency, rate or extent of protein import.
regulation of protein stability	Any process that affects the structure and integrity of a protein, altering the likelihood of its degradation or aggregation.
regulation of protein-containing complex assembly	Any process that modulates the frequency, rate or extent of protein complex assembly.
response to unfolded protein	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an unfolded protein stimulus.
RNA splicing	The process of removing sections of the primary RNA transcript to remove sequences not present in the mature form of the RNA and joining the remaining sections to form the mature form of the RNA.
slow axonal transport	The directed slow movement of non-membranous molecules in nerve cell axons. It is comprised of a Slow Component a (SCa) and a Slow Component b (SCb) which differ in transport rates and protein composition.
vesicle-mediated transport	A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane.

64 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P11484	SSB1	Ribosome-associated molecular chaperone SSB1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
P40150	SSB2	Ribosome-associated molecular chaperone SSB2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
P22202	SSA4	Heat shock protein SSA4	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
P09435	SSA3	Heat shock protein SSA3	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
P10592	SSA2	Heat shock protein SSA2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
P10591	SSA1	Heat shock protein SSA1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
Q27975	HSPA1A	Heat shock 70 kDa protein 1A	Bos taurus (Bovine)	SS
Q2YDD0	HSPA14	Heat shock 70 kDa protein 14	Bos taurus (Bovine)	SS
P0CB32	HSPA1L	Heat shock 70 kDa protein 1-like	Bos taurus (Bovine)	SS
P19120	HSPA8	Heat shock cognate 71 kDa protein	Bos taurus (Bovine)	SS
E1C2P3	HSPA14	Heat shock 70 kDa protein 14	Gallus gallus (Chicken)	SS
P08106		Heat shock 70 kDa protein	Gallus gallus (Chicken)	SS
O73885	HSPA8	Heat shock cognate 71 kDa protein	Gallus gallus (Chicken)	SS
P77319	hscC	Chaperone protein HscC	Escherichia coli (strain K12)	PR
P0A6Y8	dnaK	Chaperone protein DnaK	Escherichia coli (strain K12)	SS
P02825	Hsp70Ab	Major heat shock 70 kDa protein Ab	Drosophila melanogaster (Fruit fly)	SS
P82910	Hsp70Aa	Major heat shock 70 kDa protein Aa	Drosophila melanogaster (Fruit fly)	SS
Q9VG58	Hsp70Bbb	Major heat shock 70 kDa protein Bbb	Drosophila melanogaster (Fruit fly)	SS
Q9BIR7	Hsp70Bc	Major heat shock 70 kDa protein Bc	Drosophila melanogaster (Fruit fly)	SS
Q8INI8	Hsp70Ba	Major heat shock 70 kDa protein Ba	Drosophila melanogaster (Fruit fly)	SS
Q9BIS2	Hsp70Bb	Major heat shock 70 kDa protein Bb	Drosophila melanogaster (Fruit fly)	SS
P11146	Hsc70-2	Heat shock 70 kDa protein cognate 2	Drosophila melanogaster (Fruit fly)	SS
O97125	Hsp68	Heat shock protein 68	Drosophila melanogaster (Fruit fly)	SS
P29843	Hsc70-1	Heat shock 70 kDa protein cognate 1	Drosophila melanogaster (Fruit fly)	SS
P11147	Hsc70-4	Heat shock 70 kDa protein cognate 4	Drosophila melanogaster (Fruit fly)	SS
A2Q0Z1	HSPA8	Heat shock cognate 71 kDa protein	Equus caballus (Horse)	SS
Q96MM6	HSPA12B	Heat shock 70 kDa protein 12B	Homo sapiens (Human)	PR
O43301	HSPA12A	Heat shock 70 kDa protein 12A	Homo sapiens (Human)	PR
P0DMV9	HSPA1B	Heat shock 70 kDa protein 1B	Homo sapiens (Human)	SS
Q0VDF9	HSPA14	Heat shock 70 kDa protein 14	Homo sapiens (Human)	SS
P54652	HSPA2	Heat shock-related 70 kDa protein 2	Homo sapiens (Human)	SS
P38646	HSPA9	Stress-70 protein, mitochondrial	Homo sapiens (Human)	SS
P11021	HSPA5	Endoplasmic reticulum chaperone BiP	Homo sapiens (Human)	SS
P34931	HSPA1L	Heat shock 70 kDa protein 1-like	Homo sapiens (Human)	SS
P17066	HSPA6	Heat shock 70 kDa protein 6	Homo sapiens (Human)	SS
P0DMV8	HSPA1A	Heat shock 70 kDa protein 1A	Homo sapiens (Human)	SS
P48741	HSPA7	Putative heat shock 70 kDa protein 7	Homo sapiens (Human)	SS
P11143	HSP70	Heat shock 70 kDa protein	Zea mays (Maize)	SS
P16627	Hspa1l	Heat shock 70 kDa protein 1-like	Mus musculus (Mouse)	SS
Q99M31	Hspa14	Heat shock 70 kDa protein 14	Mus musculus (Mouse)	SS
Q8K0U4	Hspa12a	Heat shock 70 kDa protein 12A	Mus musculus (Mouse)	PR
P17156	Hspa2	Heat shock-related 70 kDa protein 2	Mus musculus (Mouse)	SS
Q61696	Hspa1a	Heat shock 70 kDa protein 1A	Mus musculus (Mouse)	SS
Q9CZJ2	Hspa12b	Heat shock 70 kDa protein 12B	Mus musculus (Mouse)	PR
P17879	Hspa1b	Heat shock 70 kDa protein 1B	Mus musculus (Mouse)	SS
P63017	Hspa8	Heat shock cognate 71 kDa protein	Mus musculus (Mouse)	SS
Q6S4N2	HSPA1B	Heat shock 70 kDa protein 1B	Sus scrofa (Pig)	SS
P14659	Hspa2	Heat shock-related 70 kDa protein 2	Rattus norvegicus (Rat)	SS
P0DMW1	Hspa1b	Heat shock 70 kDa protein 1B	Rattus norvegicus (Rat)	SS
P0DMW0	Hspa1a	Heat shock 70 kDa protein 1A	Rattus norvegicus (Rat)	SS
P55063	Hspa1l	Heat shock 70 kDa protein 1-like	Rattus norvegicus (Rat)	SS
P63018	Hspa8	Heat shock cognate 71 kDa protein	Rattus norvegicus (Rat)	SS
P09446	hsp-1	Heat shock protein hsp-1	Caenorhabditis elegans	SS
P26413	HSP70	Heat shock 70 kDa protein	Glycine max (Soybean) (Glycine hispida)	SS
Q9S9N1	HSP70-5	Heat shock 70 kDa protein 5	Arabidopsis thaliana (Mouse-ear cress)	SS
P22954	HSP70-2	Heat shock 70 kDa protein 2	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9C7X7	HSP70-18	Heat shock 70 kDa protein 18	Arabidopsis thaliana (Mouse-ear cress)	SS
O65719	HSP70-3	Heat shock 70 kDa protein 3	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9LHA8	HSP70-4	Heat shock 70 kDa protein 4	Arabidopsis thaliana (Mouse-ear cress)	SS
P22953	HSP70-1	Heat shock 70 kDa protein 1	Arabidopsis thaliana (Mouse-ear cress)	SS
P27322	HSC-2	Heat shock cognate 70 kDa protein 2	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)	SS
P24629	HSC-I	Heat shock cognate 70 kDa protein 1	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)	SS
Q5RGE6	hspa14	Heat shock 70 kDa protein 14	Danio rerio (Zebrafish) (Brachydanio rerio)	SS
Q90473	hspa8	Heat shock cognate 71 kDa protein	Danio rerio (Zebrafish) (Brachydanio rerio)	SS

10	20	30	40	50	60
MSKGPAVGID	LGTTYSCVGV	FQHGKVEIIA	NDQGNRTTPS	YVAFTDTERL	IGDAAKNQVA
70	80	90	100	110	120
MNPTNTVFDA	KRLIGRRFDD	AVVQSDMKHW	PFMVVNDAGR	PKVQVEYKGE	TKSFYPEEVS
130	140	150	160	170	180
SMVLTKMKEI	AEAYLGKTVT	NAVVTVPAYF	NDSQRQATKD	AGTIAGLNVL	RIINEPTAAA
190	200	210	220	230	240
IAYGLDKKVG	AERNVLIFDL	GGGTFDVSIL	TIEDGIFEVK	STAGDTHLGG	EDFDNRMVNH
250	260	270	280	290	300
FIAEFKRKHK	KDISENKRAV	RRLRTACERA	KRTLSSSTQA	SIEIDSLYEG	IDFYTSITRA
310	320	330	340	350	360
RFEELNADLF	RGTLDPVEKA	LRDAKLDKSQ	IHDIVLVGGS	TRIPKIQKLL	QDFFNGKELN
370	380	390	400	410	420
KSINPDEAVA	YGAAVQAAIL	SGDKSENVQD	LLLLDVTPLS	LGIETAGGVM	TVLIKRNTTI
430	440	450	460	470	480
PTKQTQTFTT	YSDNQPGVLI	QVYEGERAMT	KDNNLLGKFE	LTGIPPAPRG	VPQIEVTFDI
490	500	510	520	530	540
DANGILNVSA	VDKSTGKENK	ITITNDKGRL	SKEDIERMVQ	EAEKYKAEDE	KQRDKVSSKN
550	560	570	580	590	600
SLESYAFNMK	ATVEDEKLQG	KINDEDKQKI	LDKCNEIINW	LDKNQTAEKE	EFEHQQKELE
610	620	630	640
KVCNPIITKL	YQSAGGMPGG	MPGGFPGGGA	PPSGGASSGP	TIEEVD