P10824

EV
Gene name
Gnai1 (Gnai-1)
Protein name
Guanine nucleotide-binding protein G subunit alpha-1
Names
i, Adenylate cyclase-inhibiting G alpha protein
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:25686
EC number
Protein Class
GTP-BINDING PROTEIN ALPHA SUBUNIT (PTHR10218)

Descriptions

The Gα subunits of heterotrimeric G proteins are GTP hydrolyases which, upon receptor activation, bind to GTP and regulate effector molecules. Gα-catalyzed hydrolysis of GTP to GDP releases the Gα-GDP product complex from effector and allows its sequestration by inhibitory Gβγ subunits. In Gia1-GppNHp complex, Gln204 of Gia1 interacts with nucleophilic water (Wnuc), participating directly in the Gia1-GTP ground state but must reorient to stabilize the transition state. RGS4, a member of the RGS family of G protein stimulatory factors, accelerates hydrolysis of GTP by Gia1 by destabilizing the ground-state conformation of Gln204. Insertion of Asn128 of RGS4 into the active site enhances catalysis by aiding in binding, orienting, and polarizing Wnuc in the pre-transition state complex. Asn128 of RGS4 displaces the side chain of Gln204 from its anti-catalytic position in the ground state, freeing it to participate in stabilization of the transition state.

Autoinhibitory domains (AIDs)

204-204 (Gln204) EV

Target domain

179-349 (P-loop containing nucleoside triphosphate hydrolase domain)

Relief mechanism

Partner binding

Assay

Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

43 structures for P10824

Entry ID Method Resolution Chain Position Source
1AGR X-ray 280 A A/D 2-354 PDB
1AS0 X-ray 200 A A 2-354 PDB
1AS2 X-ray 280 A A 2-354 PDB
1AS3 X-ray 240 A A 2-354 PDB
1BH2 X-ray 210 A A 32-346 PDB
1BOF X-ray 220 A A 2-354 PDB
1CIP X-ray 150 A A 2-354 PDB
1FQJ X-ray 202 A A/D 220-299 PDB
1FQK X-ray 230 A A/C 220-299 PDB
1GDD X-ray 220 A A 2-354 PDB
1GFI X-ray 220 A A 2-354 PDB
1GG2 X-ray 240 A A 2-354 PDB
1GIA X-ray 200 A A 2-354 PDB
1GIL X-ray 230 A A 2-354 PDB
1GIT X-ray 260 A A 2-354 PDB
1GP2 X-ray 230 A A 2-354 PDB
1SHZ X-ray 285 A PDB
1SVK X-ray 200 A A 2-354 PDB
1SVS X-ray 150 A A 2-354 PDB
2BCJ X-ray 306 A Q 1-28 PDB
2RGN X-ray 350 A A/D 1-28 PDB
2ZJY X-ray 280 A A 1-354 PDB
2ZJZ X-ray 260 A A/B 1-354 PDB
3AH8 X-ray 290 A A 2-28 PDB
3D7M X-ray 290 A A 1-354 PDB
3FFA X-ray 230 A A 1-354 PDB
3FFB X-ray 257 A A 1-354 PDB
3V00 X-ray 290 A A/B/C 220-298 PDB
4N0D X-ray 155 A A 1-354 PDB
4N0E X-ray 210 A A 1-354 PDB
4PAM X-ray 210 A A 1-354 PDB
4PAN X-ray 240 A A 1-354 PDB
4PAO X-ray 200 A A 1-354 PDB
4PAQ X-ray 200 A A 1-354 PDB
5KDL X-ray 267 A A/B 1-354 PDB
5KDO X-ray 190 A A 1-354 PDB
6M8H X-ray 207 A A 1-354 PDB
6TYL X-ray 330 A B/G 1-354 PDB
6UKT EM 387 A B 32-354 PDB
6VMS EM 380 A A 1-354 PDB
7S0F EM 296 A A 1-354 PDB
7S0G EM 386 A A 1-343 PDB
AF-P10824-F1 Predicted AlphaFoldDB

No variants for P10824

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P10824

No associated diseases with P10824

No regional properties for P10824

Type Name Position InterPro Accession
No domain, repeats, and functional sites for P10824

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Cell membrane ; Peripheral membrane protein ; Cytoplasmic side
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Cytoplasm, cell cortex
  • Membrane ; Lipid-anchor
  • Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis
  • Detected at the cleavage furrow or the midbody
  • Localized at the plasma membrane throughout mitosis
  • Colocalizes with RIC8A and RGS14 at the plasma membrane
PANTHER Family PTHR10218 GTP-BINDING PROTEIN ALPHA SUBUNIT
PANTHER Subfamily PTHR10218:SF359 GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-1
PANTHER Protein Class heterotrimeric G-protein
G-protein
PANTHER Pathway Category Nicotine pharmacodynamics pathway
GNAI
5HT1 type receptor mediated signaling pathway
G-protein
Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Gialpha
Inflammation mediated by chemokine and cytokine signaling pathway
Galphai
Endogenous cannabinoid signaling
Galpha
Gonadotropin-releasing hormone receptor pathway
gnai/o
Dopamine receptor mediated signaling pathway
Gialpha
Gonadotropin-releasing hormone receptor pathway
gnai
Muscarinic acetylcholine receptor 2 and 4 signaling pathway
G-Protein
Metabotropic glutamate receptor group III pathway
G-Protein
Opioid prodynorphin pathway
G-protein
GABA-B receptor II signaling
Gi/oalpha
PI3 kinase pathway
Galpha
Metabotropic glutamate receptor group II pathway
G-protein
Enkephalin release
G-Protein (i)
Opioid proenkephalin pathway
G-protein
Opioid proopiomelanocortin pathway
G-protein
Inflammation mediated by chemokine and cytokine signaling pathway
Galphai/q

9 GO annotations of cellular component

Name Definition
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cell cortex region The complete extent of cell cortex that underlies some some region of the plasma membrane.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
heterotrimeric G-protein complex Any of a family of heterotrimeric GTP-binding and hydrolyzing proteins; they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. Heterotrimeric G-proteins consist of three subunits; the alpha subunit contains the guanine nucleotide binding site and possesses GTPase activity; the beta and gamma subunits are tightly associated and function as a beta-gamma heterodimer; extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G protein-coupled receptors to an effector protein.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.

8 GO annotations of molecular function

Name Definition
G protein-coupled receptor binding Binding to a G protein-coupled receptor.
G protein-coupled serotonin receptor binding Binding to a metabotropic serotonin receptor.
G-protein beta/gamma-subunit complex binding Binding to a complex of G-protein beta/gamma subunits.
GDP binding Binding to GDP, guanosine 5'-diphosphate.
GTP binding Binding to GTP, guanosine triphosphate.
GTPase activating protein binding Binding to a GTPase activating protein.
GTPase activity Catalysis of the reaction
magnesium ion binding Binding to a magnesium (Mg) ion.

9 GO annotations of biological process

Name Definition
adenylate cyclase-modulating G protein-coupled receptor signaling pathway A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation or inhibition of adenylyl cyclase activity and a subsequent change in the intracellular concentration of cyclic AMP (cAMP).
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
cellular response to forskolin Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a forskolin stimulus.
G protein-coupled receptor signaling pathway The series of molecular signals initiated by a ligand binding to its receptor, in which the activated receptor promotes the exchange of GDP for GTP on the alpha-subunit of an associated heterotrimeric G-protein complex. The GTP-bound activated alpha-G-protein then dissociates from the beta- and gamma-subunits to further transmit the signal within the cell. The pathway begins with receptor-ligand interaction, and ends with regulation of a downstream cellular process. The pathway can start from the plasma membrane, Golgi or nuclear membrane.
negative regulation of synaptic transmission Any process that stops, prevents, or reduces the frequency, rate or extent of synaptic transmission, the process of communication from a neuron to a target (neuron, muscle, or secretory cell) across a synapse.
positive regulation of protein localization to cell cortex Any process that activates or increases the frequency, rate or extent of protein localization to cell cortex.
regulation of cAMP-mediated signaling Any process which modulates the frequency, rate or extent of cAMP-mediated signaling.
regulation of mitotic spindle organization Any process that modulates the rate, frequency or extent of the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.

26 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P63097 GNAI1 Guanine nucleotide-binding protein G Bos taurus (Bovine) SS
P50146 GNAI1 Guanine nucleotide-binding protein G Gallus gallus (Chicken) SS
P20353 Galphai G protein alpha i subunit Drosophila melanogaster (Fruit fly) SS
P63096 GNAI1 Guanine nucleotide-binding protein G Homo sapiens (Human) EV
B2RSH2 Gnai1 Guanine nucleotide-binding protein G Mus musculus (Mouse) SS
P93564 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum tuberosum (Potato) SS
Q6Q7Y5 Gna13 Guanine nucleotide-binding protein subunit alpha-13 Rattus norvegicus (Rat) SS
Q63210 Gna12 Guanine nucleotide-binding protein subunit alpha-12 Rattus norvegicus (Rat) SS
Q9JID2 Gna11 Guanine nucleotide-binding protein subunit alpha-11 Rattus norvegicus (Rat) SS
O88302 Gna15 Guanine nucleotide-binding protein subunit alpha-15 Rattus norvegicus (Rat) SS
P82471 Gnaq Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P38406 Gnal Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P29348 Gnat3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P04897 Gnai2 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P08753 Gnai3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P19627 Gnaz Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P59215 Gnao1 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q63803 Gnas Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q0DJ33 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Oryza sativa subsp. japonica (Rice) SS
Q9N2V6 gpa-16 Guanine nucleotide-binding protein alpha-16 subunit Caenorhabditis elegans SS
Q9BIG5 gpa-4 Guanine nucleotide-binding protein alpha-4 subunit Caenorhabditis elegans SS
P93163 GPA2 Guanine nucleotide-binding protein alpha-2 subunit Glycine max (Soybean) (Glycine hispida) SS
P49084 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Glycine max (Soybean) (Glycine hispida) SS
P18064 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Arabidopsis thaliana (Mouse-ear cress) SS
O80462 XLG1 Extra-large guanine nucleotide-binding protein 1 Arabidopsis thaliana (Mouse-ear cress) PR
P26981 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MGCTLSAEDK AAVERSKMID RNLREDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEAG
70 80 90 100 110 120
YSEEECKQYK AVVYSNTIQS IIAIIRAMGR LKIDFGDAAR ADDARQLFVL AGAAEEGFMT
130 140 150 160 170 180
AELAGVIKRL WKDSGVQACF NRSREYQLND SAAYYLNDLD RIAQPNYIPT QQDVLRTRVK
190 200 210 220 230 240
TTGIVETHFT FKDLHFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM
250 260 270 280 290 300
NRMHESMKLF DSICNNKWFT DTSIILFLNK KDLFEEKIKK SPLTICYPEY AGSNTYEEAA
310 320 330 340 350
AYIQCQFEDL NKRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD CGLF