P10687
SS
Gene name |
Plcb1 |
Protein name |
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 |
Names |
PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, PLC-I, Phospholipase C-beta-1, PLC-beta-1 |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
rno:24654 |
EC number |
3.1.4.11: Phosphoric diester hydrolases |
Protein Class |
|
Descriptions
PLCB1 catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors. Autoinhibitory region is a portion of the linker that separates the conserved X and Y boxes comprising the catalytic TIM barrel, at residues 468-539, and occludes the active site of PLCB1. The active site of a PLC isozyme toward phospholipid membranes should force the negatively charged X/Y linker away from the active site, therein relieving autoinhibition of the enzyme.
Autoinhibitory domains (AIDs)
Target domain |
316-656 (TIM barrel) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Hicks SN et al. (2008) "General and versatile autoinhibition of PLC isozymes", Molecular cell, 31, 383-94
- Lyon AM et al. (2014) "Molecular mechanisms of phospholipase C β3 autoinhibition", Structure (London, England : 1993), 22, 1844-1854
- Muralidharan K et al. (2021) "Structure and regulation of phospholipase Cβ and ε at the membrane", Chemistry and physics of lipids, 235, 105050
- Rugema NY et al. (2020) "Structure of phospholipase Cε reveals an integrated RA1 domain and previously unidentified regulatory elements", Communications biology, 3, 445
- Devkota S et al. (2015) "Scaffold Protein SLP-76 Primes PLCγ1 for Activation by ITK-Mediated Phosphorylation", Journal of molecular biology, 427, 2734-47
- Kunze K et al. (2014) "A recurrent activating PLCG1 mutation in cardiac angiosarcomas increases apoptosis resistance and invasiveness of endothelial cells", Cancer research, 74, 6173-83
- DeBell K et al. (2007) "Intramolecular regulation of phospholipase C-gamma1 by its C-terminal Src homology 2 domain", Molecular and cellular biology, 27, 854-63
- Xie Q et al. (2013) "Substrate recognition of PLCγ1 via a specific docking surface on Itk", Journal of molecular biology, 425, 683-96
- Charpentier TH et al. (2014) "Membrane-induced allosteric control of phospholipase C-β isozymes", The Journal of biological chemistry, 289, 29545-57
- Lyon AM et al. (2011) "An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation", Nature structural & molecular biology, 18, 999-1005
Autoinhibited structure
Activated structure
1 structures for P10687
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P10687-F1 | Predicted | AlphaFoldDB |
1 variants for P10687
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs8170379 | 496 | V>M | No | Ensembl |
No associated diseases with P10687
8 regional properties for P10687
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 490 - 775 | IPR000719 |
| domain | S-locus glycoprotein domain | 205 - 311 | IPR000858 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 492 - 758 | IPR001245 |
| domain | Bulb-type lectin domain | 23 - 173 | IPR001480 |
| domain | PAN/Apple domain | 333 - 415 | IPR003609 |
| active_site | Serine/threonine-protein kinase, active site | 611 - 623 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 496 - 518 | IPR017441 |
| domain | S-locus receptor kinase, C-terminal | 762 - 804 | IPR021820 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.1.4.11 | Phosphoric diester hydrolases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
11 GO annotations of cellular component
| Name | Definition |
|---|---|
| chromatin | The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| GABA-ergic synapse | A synapse that uses GABA as a neurotransmitter. These synapses are typically inhibitory. |
| glutamatergic synapse | A synapse that uses glutamate as a neurotransmitter. |
| membrane | A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it. |
| nuclear membrane | Either of the lipid bilayers that surround the nucleus and form the nuclear envelope; excludes the intermembrane space. |
| nuclear speck | A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| postsynapse | The part of a synapse that is part of the post-synaptic cell. |
| protein-containing complex | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| calcium ion binding | Binding to a calcium ion (Ca2+). |
| calmodulin binding | Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states. |
| enzyme binding | Binding to an enzyme, a protein with catalytic activity. |
| GTPase activator activity | Binds to and increases the activity of a GTPase, an enzyme that catalyzes the hydrolysis of GTP. |
| identical protein binding | Binding to an identical protein or proteins. |
| lamin binding | Binding to lamin; any of a group of intermediate-filament proteins that form the fibrous matrix on the inner surface of the nuclear envelope. |
| phosphatidylinositol phospholipase C activity | Catalysis of the reaction: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1,2-diacylglycerol + 1D-myo-inositol 1,4,5-trisphosphate + H(+). |
| phosphatidylinositol-4,5-bisphosphate binding | Binding to phosphatidylinositol-4,5-bisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 4' and 5' positions. |
40 GO annotations of biological process
| Name | Definition |
|---|---|
| activation of meiosis involved in egg activation | Any process that starts the inactive process of meiosis in an egg after the egg has been fertilized or physiologically activated. Eggs generally arrest in meiosis and complete the process after activation. |
| brain development | The process whose specific outcome is the progression of the brain over time, from its formation to the mature structure. Brain development begins with patterning events in the neural tube and ends with the mature structure that is the center of thought and emotion. The brain is responsible for the coordination and control of bodily activities and the interpretation of information from the senses (sight, hearing, smell, etc.). |
| cellular response to fluoride | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a fluoride stimulus. |
| cellular response to glyceraldehyde | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glyceraldehyde stimulus. |
| cellular response to ionomycin | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ionomycin stimulus. |
| cellular response to vasopressin | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a vasopressin stimulus. |
| cerebral cortex development | The progression of the cerebral cortex over time from its initial formation until its mature state. The cerebral cortex is the outer layered region of the telencephalon. |
| fat cell differentiation | The process in which a relatively unspecialized cell acquires specialized features of an adipocyte, an animal connective tissue cell specialized for the synthesis and storage of fat. |
| G protein-coupled acetylcholine receptor signaling pathway | A G protein-coupled receptor signaling pathway initiated by a ligand binding to an acetylcholine receptor on the surface of a target cell, and ends with regulation of a downstream cellular process, e.g. transcription. |
| G2/M transition of mitotic cell cycle | The mitotic cell cycle transition by which a cell in G2 commits to M phase. The process begins when the kinase activity of M cyclin/CDK complex reaches a threshold high enough for the cell cycle to proceed. This is accomplished by activating a positive feedback loop that results in the accumulation of unphosphorylated and active M cyclin/CDK complex. |
| glutamate receptor signaling pathway | The series of molecular signals initiated by the binding of glutamate to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| inositol trisphosphate metabolic process | The chemical reactions and pathways involving myo-inositol phosphate, 1,2,3,4,5,6-cyclohexanehexol, with three phosphate groups attached. |
| insulin-like growth factor receptor signaling pathway | The series of molecular signals initiated by a ligand binding to an insulin-like growth factor receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| interleukin-1-mediated signaling pathway | The series of molecular signals initiated by interleukin-1 binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| interleukin-12-mediated signaling pathway | The series of molecular signals initiated by interleukin-12 binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| interleukin-15-mediated signaling pathway | The series of molecular signals initiated by interleukin-15 binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| learning | Any process in an organism in which a relatively long-lasting adaptive behavioral change occurs as the result of experience. |
| memory | The activities involved in the mental information processing system that receives (registers), modifies, stores, and retrieves informational stimuli. The main stages involved in the formation and retrieval of memory are encoding (processing of received information by acquisition), storage (building a permanent record of received information as a result of consolidation) and retrieval (calling back the stored information and use it in a suitable way to execute a given task). |
| negative regulation of monocyte extravasation | Any process that stops, prevents or reduces the frequency, rate or extent of monocyte extravasation. |
| negative regulation of transcription, DNA-templated | Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription. |
| phosphatidylinositol catabolic process | The chemical reactions and pathways resulting in the breakdown of phosphatidylinositol, any glycophospholipid with its sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol. |
| phosphatidylinositol metabolic process | The chemical reactions and pathways involving phosphatidylinositol, any glycophospholipid in which a sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol. |
| phosphatidylinositol-mediated signaling | The series of molecular signals in which a cell uses a phosphatidylinositol-mediated signaling to convert a signal into a response. Phosphatidylinositols include phosphatidylinositol (PtdIns) and its phosphorylated derivatives. |
| positive regulation of acrosome reaction | Any process that activates or increases the frequency, rate or extent of the acrosome reaction. |
| positive regulation of CD24 production | Any process that activates or increases the frequency, rate or extent of CD24 biosynthetic process. |
| positive regulation of developmental growth | Any process that activates, maintains or increases the rate of developmental growth. |
| positive regulation of embryonic development | Any process that activates or increases the frequency, rate or extent of embryonic development. |
| positive regulation of G1/S transition of mitotic cell cycle | Any signalling pathway that increases or activates a cell cycle cyclin-dependent protein kinase to modulate the switch from G1 phase to S phase of the mitotic cell cycle. |
| positive regulation of interleukin-12 production | Any process that activates or increases the frequency, rate, or extent of interleukin-12 production. |
| positive regulation of JNK cascade | Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the JNK cascade. |
| positive regulation of myoblast differentiation | Any process that activates or increases the frequency, rate or extent of myoblast differentiation. A myoblast is a mononucleate cell type that, by fusion with other myoblasts, gives rise to the myotubes that eventually develop into skeletal muscle fibers. |
| positive regulation of transcription, DNA-templated | Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription. |
| postsynaptic modulation of chemical synaptic transmission | Any process, acting in the postsynapse that results in modulation of chemical synaptic transmission. |
| regulation of establishment of endothelial barrier | Any process that modulates the frequency, rate or extent of establishment of endothelial barrier. |
| regulation of fertilization | Any process that modulates the rate, frequency or extent of fertilization. Fertilization is the union of gametes of opposite sexes during the process of sexual reproduction to form a zygote. It involves the fusion of the gametic nuclei (karyogamy) and cytoplasm (plasmogamy). |
| regulation of G protein-coupled receptor signaling pathway | Any process that modulates the frequency, rate or extent of G protein-coupled receptor signaling pathway. |
| regulation of retrograde trans-synaptic signaling by endocanabinoid | Any process that modulates the frequency, rate or extent of retrograde trans-synaptic signaling by an endocannabinoid. |
| response to monosaccharide | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a monosaccharide stimulus. |
| response to organonitrogen compound | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an organonitrogen stimulus. An organonitrogen compound is formally a compound containing at least one carbon-nitrogen bond. |
| response to peptide hormone | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a peptide hormone stimulus. A peptide hormone is any of a class of peptides that are secreted into the blood stream and have endocrine functions in living animals. |
23 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P32383 | PLC1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P10894 | PLCB1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | Bos taurus (Bovine) | SS |
| Q00722 | PLCB2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 | Homo sapiens (Human) | EV |
| Q15147 | PLCB4 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 | Homo sapiens (Human) | PR |
| Q01970 | PLCB3 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 | Homo sapiens (Human) | EV |
| Q9NQ66 | PLCB1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | Homo sapiens (Human) | EV |
| P51432 | Plcb3 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 | Mus musculus (Mouse) | SS |
| A3KGF7 | Plcb2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 | Mus musculus (Mouse) | PR |
| Q9Z1B3 | Plcb1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | Mus musculus (Mouse) | SS |
| P24135 | Plcg2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 | Rattus norvegicus (Rat) | SS |
| P10686 | Plcg1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 | Rattus norvegicus (Rat) | EV |
| O89040 | Plcb2 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 | Rattus norvegicus (Rat) | PR |
| Q99P84 | Plce1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 | Rattus norvegicus (Rat) | EV |
| P10688 | Plcd1 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 | Rattus norvegicus (Rat) | SS |
| Q99JE6 | Plcb3 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 | Rattus norvegicus (Rat) | SS |
| G5EBH0 | egl-8 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 | Caenorhabditis elegans | SS |
| Q8GV43 | PLC6 | Phosphoinositide phospholipase C 6 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q944C2 | PLC5 | Phosphoinositide phospholipase C 5 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q6NMA7 | PLC9 | Phosphoinositide phospholipase C 9 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9STZ3 | PLC8 | Phosphoinositide phospholipase C 8 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q56W08 | PLC3 | Phosphoinositide phospholipase C 3 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q39032 | PLC1 | Phosphoinositide phospholipase C 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q944C1 | PLC4 | Phosphoinositide phospholipase C 4 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAGAQPGVHA | LQLKPVCVSD | SLKKGTKFVK | WDDDSTIVTP | IILRTDPQGF | FFYWTDQNKE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| TELLDLSLVK | DARCGKHAKA | PKDPKLRELL | DVGNIGHLEQ | RMITVVYGPD | LVNISHLNLV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| AFQEEVAKEW | TNEVFSLATN | LLAQNMSRDA | FLEKAYTKLK | LQVTPEGRIP | LKNIYRLFSA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| DRKRVETALE | ACSLPSSRND | SIPQEDFTPD | VYRVFLNNLC | PRPEIDNIFS | EFGAKSKPYL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| TVDQMMDFIN | LKQRDPRLNE | ILYPPLKQEQ | VQVLIEKYEP | NSSLAKKGQM | SVDGFMRYLS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| GEENGVVSPE | KLDLNEDMSQ | PLSHYFINSS | HNTYLTAGQL | AGNSSVEMYR | QVLLSGCRCV |
| 370 | 380 | 390 | 400 | 410 | 420 |
| ELDCWKGRTA | EEEPVITHGF | TMTTEISFKE | VIEAIAECAF | KTSPFPILLS | FENHVDSPKQ |
| 430 | 440 | 450 | 460 | 470 | 480 |
| QAKMAEYCRL | IFGDALLMEP | LEKYPLESGV | PLPSPMDLMY | KILVKNKKKS | HKSSEGSGKK |
| 490 | 500 | 510 | 520 | 530 | 540 |
| KLSEQASNTY | SDSSSVFEPS | SPGAGEADTE | SDDDDDDDDC | KKSSMDEGTA | GSEAMATEEM |
| 550 | 560 | 570 | 580 | 590 | 600 |
| SNLVNYIQPV | KFESFETSKK | RNKSFEMSSF | VETKGLEQLT | KSPVEFVEYN | KMQLSRIYPK |
| 610 | 620 | 630 | 640 | 650 | 660 |
| GTRVDSSNYM | PQLFWNAGCQ | MVALNFQTVD | LAMQINMGMY | EYNGKSGYRL | KPEFMRRPDK |
| 670 | 680 | 690 | 700 | 710 | 720 |
| HFDPFTEGIV | DGIVANTLSV | KIISGQFLSD | KKVGTYVEVD | MFGLPVDTRR | KAFKTKTSQG |
| 730 | 740 | 750 | 760 | 770 | 780 |
| NAVNPVWEEE | PIVFKKVVLP | SLACLRIAAY | EEGGKFIGHR | ILPVQAIRPG | YHYICLRNER |
| 790 | 800 | 810 | 820 | 830 | 840 |
| NQPLMLPAVF | VYIEVKDYVP | DTYADVIEAL | SNPIRYVNLM | EQRAKQLAAL | TLEDEEEVKK |
| 850 | 860 | 870 | 880 | 890 | 900 |
| EADPGETSSE | APSETRTTPA | ENGVNHTATL | APKPPSQAPH | SQPAPGSVKA | PAKTEDLIQS |
| 910 | 920 | 930 | 940 | 950 | 960 |
| VLTEVEAQTI | EELKQQKSFV | KLQKKHYKEM | KDLVKRHHKK | TTELIKEHTT | KYNEIQNDYL |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| RRRAALEKSA | KKDSKKKSEP | SSPDHGSSAI | EQDLAALDAE | MTQKLIDLKD | KQQQQLLNLR |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| QEQYYSEKYQ | KREHIKLLIQ | KLTDVAEECQ | NNQLKKLKEI | CEKEKKELKK | KMDKKRQEKI |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| TEAKSKDKSQ | MEEEKTEMIR | SYIQEVVQYI | KRLEEAQSKR | QEKLVEKHKE | IRQQILDEKP |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| KLQMELEQEY | QDKFKRLPLE | ILEFVQEAMK | GKVSEDSNHG | SAPPSLASDP | AKVNLKSPSS |
| 1210 | |||||
| EEVQGENAGR | EFDTPL |