P10686

EV
Gene name
Plcg1
Protein name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Names
Phosphoinositide phospholipase C-gamma-1, Phospholipase C-gamma-1, PLC-gamma-1
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:25738
EC number
3.1.4.11: Phosphoric diester hydrolases
Protein Class
PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN (PTHR10336)

Descriptions

Phospholipase C gamma 1 (PLCγ1) is an enzyme that plays a crucial role in intracellular signaling pathways. It hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate second messengers. The autoinhibition of PLCγ1 is mediated by the interaction of its sPH and cSH2 domains, which form extensive autoinhibitory contacts with the core of PLCγ1, thereby blocking membrane engagement and access to the PIP2 substrate. Autoinhibition is relieved by phosphorylation at Y783.

Autoinhibitory domains (AIDs)

489-523 (sPH domain, first part) EV

Target domain

320-464 (Catalytic PLC X domain)

Relief mechanism

PTM, Partner binding

Assay

Structural analysis

895-931 (sPH domain, second part) EV

Target domain

320-464 (Catalytic PLC X domain)

Relief mechanism

PTM, Partner binding

Assay

Structural analysis

668-756 (cSH2 domain) EV

Target domain

320-464 (Catalytic PLC X domain)

Relief mechanism

PTM, Partner binding

Assay

Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

12 structures for P10686

Entry ID Method Resolution Chain Position Source
1Y0M X-ray 120 A A 791-851 PDB
1YWO X-ray 181 A A 790-851 PDB
1YWP X-ray 160 A A 790-851 PDB
2FJL NMR - PDB
3GQI X-ray 250 A B 545-770 PDB
4K44 X-ray 170 A A/B 664-766 PDB
4K45 X-ray 150 A PDB
5EG3 X-ray 261 A B 661-773 PDB
6PBC X-ray 246 A PDB
7T8T EM 368 A A 20-1215 PDB
7Z3J X-ray 200 A PDB
AF-P10686-F1 Predicted AlphaFoldDB

No variants for P10686

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P10686

No associated diseases with P10686

14 regional properties for P10686

Type Name Position InterPro Accession
domain C2 domain 1071 - 1194 IPR000008
domain Phosphatidylinositol-specific phospholipase C, X domain 320 - 465 IPR000909
domain SH2 domain 548 - 657 IPR000980-1
domain SH2 domain 666 - 756 IPR000980-2
domain SH3 domain 791 - 851 IPR001452
domain Phospholipase C, phosphatidylinositol-specific, Y domain 953 - 1070 IPR001711
domain Pleckstrin homology domain 27 - 144 IPR001849-1
domain Pleckstrin homology domain 489 - 680 IPR001849-2
domain Pleckstrin homology domain 804 - 933 IPR001849-3
domain EF-hand domain 152 - 187 IPR002048
binding_site EF-Hand 1, calcium-binding site 165 - 177 IPR018247
domain PLC-gamma, C-terminal SH2 domain 663 - 765 IPR035023
domain PLC-gamma, N-terminal SH2 domain 545 - 649 IPR035024
domain 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, SH3 domain 791 - 850 IPR035724

Functions

Description
EC Number 3.1.4.11 Phosphoric diester hydrolases
Subcellular Localization
  • Cell projection, lamellipodium
  • Cell projection, ruffle
  • Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment
PANTHER Family PTHR10336 PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN
PANTHER Subfamily PTHR10336:SF173 1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE GAMMA-1
PANTHER Protein Class phospholipase
PANTHER Pathway Category VEGF signaling pathway
PLC-gamma
Inflammation mediated by chemokine and cytokine signaling pathway
PLCbetagamma
FGF signaling pathway
PLCgamma
Axon guidance mediated by netrin
Phospholipase C
Thyrotropin-releasing hormone receptor signaling pathway
PLC
Angiogenesis
PLC-gamma
EGF receptor signaling pathway
PLCgamma
PDGF signaling pathway
PLCgamma
T cell activation
PLC-gamma
Oxytocin receptor mediated signaling pathway
PLC beta
Histamine H1 receptor mediated signaling pathway
PLC
5HT2 type receptor mediated signaling pathway
PLC
CCKR signaling map
PLCgamma1

11 GO annotations of cellular component

Name Definition
cell projection A prolongation or process extending from a cell, e.g. a flagellum or axon.
cell-cell junction A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects.
clathrin-coated vesicle A vesicle with a coat formed of clathrin connected to the membrane via one of the clathrin adaptor complexes.
COP9 signalosome A protein complex that catalyzes the deneddylation of proteins, including the cullin component of SCF ubiquitin E3 ligase; deneddylation increases the activity of cullin family ubiquitin ligases. The signalosome is involved in many regulatory process, including some which control development, in many species; also regulates photomorphogenesis in plants; in many species its subunits are highly similar to those of the proteasome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
glutamatergic synapse A synapse that uses glutamate as a neurotransmitter.
lamellipodium A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
ruffle Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork.
ruffle membrane The portion of the plasma membrane surrounding a ruffle.
Schaffer collateral - CA1 synapse A synapse between the Schaffer collateral axon of a CA3 pyramidal cell and a CA1 pyramidal cell.

9 GO annotations of molecular function

Name Definition
calcium ion binding Binding to a calcium ion (Ca2+).
calcium-dependent phospholipase C activity Catalysis of the reaction: a phosphatidylcholine + H2O = 1,2-diacylglycerol + choline phosphate. This reaction requires Ca2+.
glutamate receptor binding Binding to a glutamate receptor.
insulin receptor binding Binding to an insulin receptor.
neurotrophin TRKA receptor binding Binding to a neurotrophin TRKA receptor.
phosphatidylinositol phospholipase C activity Catalysis of the reaction: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1,2-diacylglycerol + 1D-myo-inositol 1,4,5-trisphosphate + H(+).
phosphoprotein binding Binding to a phosphorylated protein.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
receptor tyrosine kinase binding Binding to a receptor that possesses protein tyrosine kinase activity.

24 GO annotations of biological process

Name Definition
calcium ion transport The directed movement of calcium (Ca) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
calcium-mediated signaling Any intracellular signal transduction in which the signal is passed on within the cell via calcium ions.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. Cell migration is a central process in the development and maintenance of multicellular organisms.
cellular response to epidermal growth factor stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an epidermal growth factor stimulus.
epidermal growth factor receptor signaling pathway The series of molecular signals initiated by binding of a ligand to the tyrosine kinase receptor EGFR (ERBB1) on the surface of a cell. The pathway ends with regulation of a downstream cellular process, e.g. transcription.
in utero embryonic development The process whose specific outcome is the progression of the embryo in the uterus over time, from formation of the zygote in the oviduct, to birth. An example of this process is found in Mus musculus.
inositol trisphosphate biosynthetic process The chemical reactions and pathways resulting in the formation of inositol trisphosphate, 1,2,3,4,5,6-cyclohexanehexol, with three phosphate groups attached.
modulation of chemical synaptic transmission Any process that modulates the frequency or amplitude of synaptic transmission, the process of communication from a neuron to a target (neuron, muscle, or secretory cell) across a synapse. Amplitude, in this case, refers to the change in postsynaptic membrane potential due to a single instance of synaptic transmission.
phosphatidylinositol metabolic process The chemical reactions and pathways involving phosphatidylinositol, any glycophospholipid in which a sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol.
phospholipid catabolic process The chemical reactions and pathways resulting in the breakdown of phospholipids, any lipid containing phosphoric acid as a mono- or diester.
positive regulation of angiogenesis Any process that activates or increases angiogenesis.
positive regulation of blood vessel endothelial cell migration Any process that activates or increases the frequency, rate or extent of the migration of the endothelial cells of blood vessels.
positive regulation of endothelial cell apoptotic process Any process that activates or increases the frequency, rate or extent of endothelial cell apoptotic process.
positive regulation of epithelial cell migration Any process that activates or increases the frequency, rate or extent of epithelial cell migration.
positive regulation of release of sequestered calcium ion into cytosol Any process that activates or increases the frequency, rate or extent of the release into the cytosolic compartment of calcium ions sequestered in the endoplasmic reticulum or mitochondria.
positive regulation of vascular endothelial cell proliferation Any process that activates or increases the frequency, rate or extent of vascular endothelial cell proliferation.
protein secretion The controlled release of proteins from a cell.
regulation of store-operated calcium channel activity Any process that modulates the frequency, rate or extent of store-operated calcium channel activity.
response to gravity Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a gravitational stimulus.
response to hydrogen peroxide Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hydrogen peroxide (H2O2) stimulus.
response to morphine Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a morphine stimulus. Morphine is an opioid alkaloid, isolated from opium, with a complex ring structure.
response to organonitrogen compound Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an organonitrogen stimulus. An organonitrogen compound is formally a compound containing at least one carbon-nitrogen bond.
signal transduction The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
T cell receptor signaling pathway The series of molecular signals initiated by the cross-linking of an antigen receptor on a T cell.

17 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P08487 PLCG1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Bos taurus (Bovine) EV SS
P16885 PLCG2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 Homo sapiens (Human) SS
P19174 PLCG1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Homo sapiens (Human) EV
Q62077 Plcg1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Mus musculus (Mouse) SS
P24135 Plcg2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 Rattus norvegicus (Rat) SS
O89040 Plcb2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 Rattus norvegicus (Rat) PR
P10687 Plcb1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 Rattus norvegicus (Rat) SS
Q99P84 Plce1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 Rattus norvegicus (Rat) EV
P10688 Plcd1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 Rattus norvegicus (Rat) SS
Q99JE6 Plcb3 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Rattus norvegicus (Rat) SS
Q39032 PLC1 Phosphoinositide phospholipase C 1 Arabidopsis thaliana (Mouse-ear cress) PR
Q56W08 PLC3 Phosphoinositide phospholipase C 3 Arabidopsis thaliana (Mouse-ear cress) PR
Q6NMA7 PLC9 Phosphoinositide phospholipase C 9 Arabidopsis thaliana (Mouse-ear cress) PR
Q8GV43 PLC6 Phosphoinositide phospholipase C 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q944C2 PLC5 Phosphoinositide phospholipase C 5 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STZ3 PLC8 Phosphoinositide phospholipase C 8 Arabidopsis thaliana (Mouse-ear cress) PR
Q944C1 PLC4 Phosphoinositide phospholipase C 4 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MAGVGTPCAN GCGPSAPSEA EVLHLCRSLE VGTVMTLFYS KKSQRPERKT FQVKLETRQI
70 80 90 100 110 120
TWSRGADKIE GSIDIREIKE IRPGKTSRDF DRYQEDPAFR PDQSHCFVIL YGMEFRLKTL
130 140 150 160 170 180
SLQATSEDEV NMWIKGLTWL MEDTLQAATP LQIERWLRKQ FYSVDRNRED RISAKDLKNM
190 200 210 220 230 240
LSQVNYRVPN MRFLRERLTD FEQRSGDITY GQFAQLYRSL MYSAQKTMDL PFLETNTLRT
250 260 270 280 290 300
GERPELCQVS LSEFQQFLLE YQGELWAVDR LQVQEFMLSF LRDPLREIEE PYFFLDELVT
310 320 330 340 350 360
FLFSKENSVW NSQLDAVCPE TMNNPLSHYW ISSSHNTYLT GDQFSSESSL EAYARCLRMG
370 380 390 400 410 420
CRCIELDCWD GPDGMPVIYH GHTLTTKIKF SDVLHTIKEH AFVASEYPVI LSIEDHCSIA
430 440 450 460 470 480
QQRNMAQHFR KVLGDTLLTK PVDIAADGLP SPNQLKRKIL IKHKKLAEGS AYEEVPTSVM
490 500 510 520 530 540
YSENDISNSI KNGILYLEDP VNHEWYPHYF VLTSSKIYYS EETSSDQGNE DEEEPKEASG
550 560 570 580 590 600
STELHSSEKW FHGKLGAGRD GRHIAERLLT EYCIETGAPD GSFLVRESET FVGDYTLSFW
610 620 630 640 650 660
RNGKVQHCRI HSRQDAGTPK FFLTDNLVFD SLYDLITHYQ QVPLRCNEFE MRLSEPVPQT
670 680 690 700 710 720
NAHESKEWYH ASLTRAQAEH MLMRVPRDGA FLVRKRNEPN SYAISFRAEG KIKHCRVQQE
730 740 750 760 770 780
GQTVMLGNSE FDSLVDLISY YEKHPLYRKM KLRYPINEEA LEKIGTAEPD YGALYEGRNP
790 800 810 820 830 840
GFYVEANPMP TFKCAVKALF DYKAQREDEL TFTKSAIIQN VEKQDGGWWR GDYGGKKQLW
850 860 870 880 890 900
FPSNYVEEMI NPAILEPERE HLDENSPLGD LLRGVLDVPA CQIAIRPEGK NNRLFVFSIS
910 920 930 940 950 960
MPSVAQWSLD VAADSQEELQ DWVKKIREVA QTADARLTEG KMMERRKKIA LELSELVVYC
970 980 990 1000 1010 1020
RPVPFDEEKI GTERACYRDM SSFPETKAEK YVNKAKGKKF LQYNRLQLSR IYPKGQRLDS
1030 1040 1050 1060 1070 1080
SNYDPLPMWI CGSQLVALNF QTPDKPMQMN QALFMAGGHC GYVLQPSTMR DEAFDPFDKS
1090 1100 1110 1120 1130 1140
SLRGLEPCVI CIEVLGARHL PKNGRGIVCP FVEIEVAGAE YDSTKQKTEF VVDNGLNPVW
1150 1160 1170 1180 1190 1200
PAKPFHFQIS NPEFAFLRFV VYEEDMFSDQ NFLAQATFPV KGLKTGYRAV PLKNNYSEDL
1210 1220 1230 1240 1250 1260
ELASLLIKID IFPAKENGDL SPFSGTSLRE RASDASSQLF HVRAREGSFE ARYQQPFEDF
1270 1280
RISQEHLADH FDSRERRAPR RTRVNGDNRL