P0CAV4
Gene name |
skgA (CC_0694) |
Protein name |
HTH-type transcriptional regulator SkgA |
Names |
Stationary-phase regulation of KatG protein |
Species |
Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) |
KEGG Pathway |
ccr:CC_0694 |
EC number |
|
Protein Class |
REDOX-CYCLING DRUG-SENSING TRANSCRIPTIONAL ACTIVATOR SOXR (PTHR30204) |
Descriptions
SkgA belongs to the TipA class of transcription factors, which constitutes minimal autoregulated multidrug resistance (MDR) against diverse antibiotics. Upon binding of antibiotics to the TipAS domain within SkgA, SkgA is able to induce transcription of genes involved in multidrug resistance such as efflux pumps and antibiotics sequester proteins. The autoinhibition mechanism involves the a6–a7 region of the TipAS domain, which, in the unliganded state, interacts with the DNA-binding domain to stabilize the dimeric interface and hinder promoter DNA binding. This interaction prevents transcriptional activation by sterically blocking DNA access to the DNA-binding domain.
Autoinhibitory domains (AIDs)
Target domain |
6-73 (MerR-type HTH domain) |
Relief mechanism |
Ligand binding |
Assay |
Structural analysis |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
2 structures for P0CAV4
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 7CLA | X-ray | 250 A | A | 1-255 | PDB |
| AF-P0CAV4-F1 | Predicted | AlphaFoldDB |
No variants for P0CAV4
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P0CAV4 | |||||
No associated diseases with P0CAV4
5 regional properties for P0CAV4
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | SNF2, N-terminal | 43 - 321 | IPR000330 |
| domain | Helicase, C-terminal domain-like | 342 - 507 | IPR001650 |
| domain | Macro domain | 709 - 900 | IPR002589 |
| domain | Helicase superfamily 1/2, ATP-binding domain | 36 - 224 | IPR014001 |
| domain | SNF2/RAD5-like, C-terminal helicase domain | 340 - 464 | IPR049730 |
Functions
| Description | ||
|---|---|---|
| EC Number | ||
| Subcellular Localization |
|
|
| PANTHER Family | PTHR30204 | REDOX-CYCLING DRUG-SENSING TRANSCRIPTIONAL ACTIVATOR SOXR |
| PANTHER Subfamily | PTHR30204:SF90 | HTH-TYPE TRANSCRIPTIONAL ACTIVATOR TIPA |
| PANTHER Protein Class |
helix-turn-helix transcription factor
winged helix/forkhead transcription factor |
|
| PANTHER Pathway Category | No pathway information available | |
No GO annotations of cellular component
| Name | Definition |
|---|---|
| No GO annotations for cellular component |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| DNA-binding transcription factor activity | A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. |
No GO annotations of biological process
| Name | Definition |
|---|---|
| No GO annotations for biological process |
No homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| No homologous proteins | ||||
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSVYTVKQMA | RLSGVSVRAL | HHYDAIGLLK | PRAVGANGYR | YYDRQDLLRL | QQILFHRALE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| TPLKDIQAAL | DQPGFDLAAA | LRAQRERLAA | QAERYARLVD | VVDRTLADLE | GDETMDDKHL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| FEGFDPEKQA | RHEAWLVEHY | GDEATRRIAD | AKAGMKSWGK | KDWSQFQEEA | KAIEHDLAKA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LTQGLPVDSA | PVTAIMRRHW | AWVGRSWNRE | PTPDAFAGLG | HLYQANPEFT | ARYEAIAPGL |
| 250 | |||||
| TEYFSEAMRA | FARGR |