P0C865
SS
Gene name |
Mapk7 (Bmk1, Erk5) |
Protein name |
Mitogen-activated protein kinase 7 |
Names |
MAP kinase 7 , MAPK 7 , EC 2.7.11.24 , Big MAP kinase 1 , BMK-1 , Extracellular signal-regulated kinase 5 , ERK-5 |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
|
EC number |
2.7.11.24: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
MAPK1 encodes Mitogen-activated protein kinase 1, and is involved in diverse cellular responses by phosphorylating cytoplasmic and nuclear proteins. Unlike other MAP kinases, MAPK1 activates via dual phosphorylation of Thr-186 and Tyr-193 within the activation lip, and mutations at Q105 alone were sufficient for autoactivation. Additionally, in the inactive form, L76, Q10 and I87 form a structural unit that restrains intramolecular autophosphorylation by preventing interactions between activation lip residues and the catalytic base (D149) needed for phosphoryl transfer. Mutations at L76, I87 and additionally S153 increased basal specific activity via autophosphorylation of Thr-186 and Tyr-193.
Autoinhibitory domains (AIDs)
Target domain |
55-347 (Protein kinase domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
199-226 (Activation loop from InterPro)
Target domain |
55-347 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
- Emrick MA et al. (2006) "The gatekeeper residue controls autoactivation of ERK2 via a pathway of intramolecular connectivity", Proceedings of the National Academy of Sciences of the United States of America, 103, 18101-6
- Emrick MA et al. (2001) "Constitutive activation of extracellular signal-regulated kinase 2 by synergistic point mutations", The Journal of biological chemistry, 276, 46469-79
Autoinhibited structure
Activated structure
1 structures for P0C865
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P0C865-F1 | Predicted | AlphaFoldDB |
No variants for P0C865
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P0C865 | |||||
No associated diseases with P0C865
4 regional properties for P0C865
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 55 - 347 | IPR000719 |
| conserved_site | Mitogen-activated protein (MAP) kinase, conserved site | 90 - 194 | IPR003527 |
| active_site | Serine/threonine-protein kinase, active site | 178 - 190 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 61 - 85 | IPR017441 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.24 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| PML body | A class of nuclear body; they react against SP100 auto-antibodies (PML, promyelocytic leukemia); cells typically contain 10-30 PML bodies per nucleus; alterations in the localization of PML bodies occurs after viral infection. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| MAP kinase activity | Catalysis of the reaction |
| mitogen-activated protein kinase binding | Binding to a mitogen-activated protein kinase. |
| protein kinase activity | Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction |
| protein serine/threonine kinase activity | Catalysis of the reactions |
23 GO annotations of biological process
| Name | Definition |
|---|---|
| calcineurin-NFAT signaling cascade | Any intracellular signal transduction in which the signal is passed on within the cell by activation of a member of the NFAT protein family as a consequence of NFAT dephosphorylation by Ca(2+)-activated calcineurin. The cascade begins with calcium-dependent activation of the phosphatase calcineurin. Calcineurin dephosphorylates multiple phosphoserine residues on NFAT, resulting in the translocation of NFAT to the nucleus. The cascade ends with regulation of transcription by NFAT. The calcineurin-NFAT cascade lies downstream of many cell surface receptors, including G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs) that signal to mobilize calcium ions (Ca2+). |
| cell cycle | The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division. |
| cell differentiation | The cellular developmental process in which a relatively unspecialized cell, e.g. embryonic or regenerative cell, acquires specialized structural and/or functional features that characterize a specific cell. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state. |
| cellular response to growth factor stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a growth factor stimulus. |
| cellular response to hydrogen peroxide | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hydrogen peroxide (H2O2) stimulus. |
| cellular response to laminar fluid shear stress | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a laminar fluid shear stress stimulus. Laminar fluid flow is the force acting on an object in a system where the fluid is moving across a solid surface in parallel layers. |
| cellular response to transforming growth factor beta stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a transforming growth factor beta stimulus. |
| ERK5 cascade | An intracellular protein kinase cascade containing at least ERK5 (also called BMK1; a MAPK), a MEK (a MAPKK) and a MAP3K. The cascade can also contain an additional tier |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| MAPK cascade | An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers |
| negative regulation of apoptotic process | Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process. |
| negative regulation of calcineurin-NFAT signaling cascade | Any process that stops, prevents, or reduces the frequency, rate or extent of the calcineurin-NFAT signaling cascade. |
| negative regulation of endothelial cell apoptotic process | Any process that stops, prevents or reduces the frequency, rate or extent of endothelial cell apoptotic process. |
| negative regulation of ERK5 cascade | Any process that stops, prevents, or reduces the frequency, rate or extent of signal transduction mediated by the ERK5 cascade. |
| negative regulation of extrinsic apoptotic signaling pathway in absence of ligand | Any process that stops, prevents or reduces the frequency, rate or extent of extrinsic apoptotic signaling pathway in absence of ligand. |
| negative regulation of heterotypic cell-cell adhesion | Any process that stops, prevents, or reduces the frequency, rate, or extent of heterotypic cell-cell adhesion. |
| negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway | Any process that stops, prevents or reduces the frequency, rate or extent of an oxidative stress-induced intrinsic apoptotic signaling pathway. |
| negative regulation of response to cytokine stimulus | Any process that decreases the rate, frequency, or extent of a response to cytokine stimulus. |
| negative regulation of smooth muscle cell apoptotic process | Any process that stops, prevents, or reduces the frequency, rate, or extent of smooth muscle cell apoptotic process. |
| phosphorylation | The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. |
| positive regulation of protein metabolic process | Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways involving a protein. |
| positive regulation of transcription by RNA polymerase II | Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter. |
| regulation of angiogenesis | Any process that modulates the frequency, rate or extent of angiogenesis. |
89 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P41808 | SMK1 | Sporulation-specific mitogen-activated protein kinase SMK1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q00772 | SLT2 | Mitogen-activated protein kinase SLT2/MPK1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q3T0N5 | MAPK13 | Mitogen-activated protein kinase 13 | Bos taurus (Bovine) | SS |
| P46196 | MAPK1 | Mitogen-activated protein kinase 1 | Bos taurus (Bovine) | SS |
| A5PKJ4 | MAPK7 | Mitogen-activated protein kinase 7 | Bos taurus (Bovine) | SS |
| Q5F3W3 | MAPK6 | Mitogen-activated protein kinase 6 | Gallus gallus (Chicken) | SS |
| Q95NE7 | MAPK14 | Mitogen-activated protein kinase 14 | Pan troglodytes (Chimpanzee) | SS |
| Q9N272 | MAPK13 | Mitogen-activated protein kinase 13 | Pan troglodytes (Chimpanzee) | SS |
| Q9W354 | Erk7 | Extracellular signal-regulated kinase 7 | Drosophila melanogaster (Fruit fly) | SS |
| O61443 | p38b | Mitogen-activated protein kinase p38b | Drosophila melanogaster (Fruit fly) | SS |
| O62618 | p38a | Mitogen-activated protein kinase p38a | Drosophila melanogaster (Fruit fly) | SS |
| P83100 | p38c | Putative mitogen-activated protein kinase 14C | Drosophila melanogaster (Fruit fly) | SS |
| P40417 | rl | Mitogen-activated protein kinase ERK-A | Drosophila melanogaster (Fruit fly) | SS |
| O15264 | MAPK13 | Mitogen-activated protein kinase 13 | Homo sapiens (Human) | SS |
| P53778 | MAPK12 | Mitogen-activated protein kinase 12 | Homo sapiens (Human) | SS |
| P28482 | MAPK1 | Mitogen-activated protein kinase 1 | Homo sapiens (Human) | EV |
| Q16539 | MAPK14 | Mitogen-activated protein kinase 14 | Homo sapiens (Human) | SS |
| Q16659 | MAPK6 | Mitogen-activated protein kinase 6 | Homo sapiens (Human) | SS |
| P27361 | MAPK3 | Mitogen-activated protein kinase 3 | Homo sapiens (Human) | SS |
| Q15759 | MAPK11 | Mitogen-activated protein kinase 11 | Homo sapiens (Human) | SS |
| Q8TD08 | MAPK15 | Mitogen-activated protein kinase 15 | Homo sapiens (Human) | SS |
| P31152 | MAPK4 | Mitogen-activated protein kinase 4 | Homo sapiens (Human) | SS |
| Q13164 | MAPK7 | Mitogen-activated protein kinase 7 | Homo sapiens (Human) | SS |
| Q9Z1B7 | Mapk13 | Mitogen-activated protein kinase 13 | Mus musculus (Mouse) | SS |
| P47811 | Mapk14 | Mitogen-activated protein kinase 14 | Mus musculus (Mouse) | SS |
| Q61532 | Mapk6 | Mitogen-activated protein kinase 6 | Mus musculus (Mouse) | SS |
| Q63844 | Mapk3 | Mitogen-activated protein kinase 3 | Mus musculus (Mouse) | SS |
| P63085 | Mapk1 | Mitogen-activated protein kinase 1 | Mus musculus (Mouse) | SS |
| Q9WUI1 | Mapk11 | Mitogen-activated protein kinase 11 | Mus musculus (Mouse) | SS |
| O08911 | Mapk12 | Mitogen-activated protein kinase 12 | Mus musculus (Mouse) | SS |
| Q80Y86 | Mapk15 | Mitogen-activated protein kinase 15 | Mus musculus (Mouse) | SS |
| Q6P5G0 | Mapk4 | Mitogen-activated protein kinase 4 | Mus musculus (Mouse) | SS |
| Q9WVS8 | Mapk7 | Mitogen-activated protein kinase 7 | Mus musculus (Mouse) | SS |
| P21708 | Mapk3 | Mitogen-activated protein kinase 3 | Rattus norvegicus (Rat) | SS |
| P27704 | Mapk6 | Mitogen-activated protein kinase 6 | Rattus norvegicus (Rat) | SS |
| P63086 | Mapk1 | Mitogen-activated protein kinase 1 | Rattus norvegicus (Rat) | SS |
| P70618 | Mapk14 | Mitogen-activated protein kinase 14 | Rattus norvegicus (Rat) | SS |
| Q63538 | Mapk12 | Mitogen-activated protein kinase 12 | Rattus norvegicus (Rat) | SS |
| Q9WTY9 | Mapk13 | Mitogen-activated protein kinase 13 | Rattus norvegicus (Rat) | SS |
| Q9Z2A6 | Mapk15 | Mitogen-activated protein kinase 15 | Rattus norvegicus (Rat) | SS |
| P20793 | Mak | Serine/threonine-protein kinase MAK | Rattus norvegicus (Rat) | PR |
| P49186 | Mapk9 | Mitogen-activated protein kinase 9 | Rattus norvegicus (Rat) | PR |
| Q63454 | Mapk4 | Mitogen-activated protein kinase 4 | Rattus norvegicus (Rat) | SS |
| Q10N20 | MPK5 | Mitogen-activated protein kinase 5 | Oryza sativa subsp. japonica (Rice) | SS |
| Q84UI5 | MPK1 | Mitogen-activated protein kinase 1 | Oryza sativa subsp. japonica (Rice) | SS |
| Q5J4W4 | MPK2 | Mitogen-activated protein kinase 2 | Oryza sativa subsp. japonica (Rice) | SS |
| Q336X9 | MPK6 | Mitogen-activated protein kinase 6 | Oryza sativa subsp. japonica (Rice) | SS |
| Q53N72 | MPK15 | Mitogen-activated protein kinase 15 | Oryza sativa subsp. japonica (Rice) | SS |
| Q5VN19 | MPK11 | Mitogen-activated protein kinase 11 | Oryza sativa subsp. japonica (Rice) | SS |
| Q5VP69 | MPK16 | Mitogen-activated protein kinase 16 | Oryza sativa subsp. japonica (Rice) | SS |
| Q5Z859 | MPK4 | Mitogen-activated protein kinase 4 | Oryza sativa subsp. japonica (Rice) | SS |
| Q5Z9J0 | MPK12 | Mitogen-activated protein kinase 12 | Oryza sativa subsp. japonica (Rice) | SS |
| Q5ZCI1 | MPK10 | Mitogen-activated protein kinase 10 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6L5F7 | MPK17 | Mitogen-activated protein kinase 17 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6Z437 | MPK3 | Mitogen-activated protein kinase 3 | Oryza sativa subsp. japonica (Rice) | SS |
| Q75KK8 | MPK14 | Mitogen-activated protein kinase 14 | Oryza sativa subsp. japonica (Rice) | SS |
| Q0E459 | MPK13 | Mitogen-activated protein kinase 13 | Oryza sativa subsp. japonica (Rice) | SS |
| Q5SN53 | MPK8 | Mitogen-activated protein kinase 8 | Oryza sativa subsp. japonica (Rice) | SS |
| Q67C40 | MPK7 | Mitogen-activated protein kinase 7 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6L5D4 | MPK9 | Mitogen-activated protein kinase 9 | Oryza sativa subsp japonica (Rice) | PR |
| Q17446 | pmk-1 | Mitogen-activated protein kinase pmk-1 | Caenorhabditis elegans | SS |
| Q11179 | mapk-15 | Mitogen-activated protein kinase 15 | Caenorhabditis elegans | SS |
| Q8MXI4 | pmk-2 | Mitogen-activated protein kinase pmk-2 | Caenorhabditis elegans | SS |
| P39745 | mpk-1 | Mitogen-activated protein kinase mpk-1 | Caenorhabditis elegans | SS |
| G5EBT1 | sma-5 | Mitogen-activated protein kinase sma-5 | Caenorhabditis elegans | SS |
| O44514 | pmk-3 | Mitogen-activated protein kinase pmk-3 | Caenorhabditis elegans | PR |
| Q9LMM5 | MPK11 | Mitogen-activated protein kinase 11 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LQQ9 | MPK13 | Mitogen-activated protein kinase 13 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q39026 | MPK6 | Mitogen-activated protein kinase 6 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q8GYQ5 | MPK12 | Mitogen-activated protein kinase 12 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q39023 | MPK3 | Mitogen-activated protein kinase 3 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M1Z5 | MPK10 | Mitogen-activated protein kinase 10 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q39024 | MPK4 | Mitogen-activated protein kinase 4 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q39025 | MPK5 | Mitogen-activated protein kinase 5 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O23236 | MPK14 | Mitogen-activated protein kinase 14 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q39021 | MPK1 | Mitogen-activated protein kinase 1 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q39022 | MPK2 | Mitogen-activated protein kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q39027 | MPK7 | Mitogen-activated protein kinase 7 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q84M93 | MPK17 | Mitogen-activated protein kinase 17 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q8W4J2 | MPK16 | Mitogen-activated protein kinase 16 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9C5C0 | MPK18 | Mitogen-activated protein kinase 18 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9C9U4 | MPK15 | Mitogen-activated protein kinase 15 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LM33 | MPK8 | Mitogen-activated protein kinase 8 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LUC3 | MPK19 | Mitogen-activated protein kinase 19 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LV37 | MPK9 | Mitogen-activated protein kinase 9 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9SJG9 | MPK20 | Mitogen-activated protein kinase 20 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O42376 | mapk12 | Mitogen-activated protein kinase 12 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| Q9DGE1 | mapk14b | Mitogen-activated protein kinase 14B | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| Q9DGE2 | mapk14a | Mitogen-activated protein kinase 14A | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAEPLKEEDG | EDGSGEPPGR | VKAEPVHTAA | SVVAKNLALL | KARSFDVTFD | VGDEYEIIET |
| 70 | 80 | 90 | 100 | 110 | 120 |
| IGNGAYGVVS | SARRRLTGQQ | VAIKKIPNAF | DVVTNAKRTL | RELKILKHFK | HDNIIAIKDI |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LRPTVPYGEF | RSVYVVLDLM | ESDLHQIIHS | SQPLTLEHVR | YFLYQLLRGL | KYMHSAQVIH |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RDLKPSNLLV | NENCELKIGD | FGMARGLCTS | PAEHQYFMTE | YVATRWYRAP | ELMLSLHEYT |
| 250 | 260 | 270 | 280 | 290 | 300 |
| QAIDLWSVGC | IFGEMLARRQ | LFPGKNYVHQ | LQLIMMVLGT | PSPAVIQAVG | AERVRAYIQS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LPPRQPVPWE | TVYPGADRQA | LSLLGRMLRF | EPSARISAAA | ALRHPFLAKY | HDPDDEPDCA |
| 370 | 380 | 390 | 400 | 410 | 420 |
| PPFDFAFDRE | ALTRERIKEA | IVAEIEDFHA | RREGIRQQIR | FQPSLQPVAS | EPVCPDVEMP |
| 430 | 440 | 450 | 460 | 470 | 480 |
| SPWAPSGDCA | MESPPPALPP | CSGPAPDTVD | LTLQPAPPAS | ELAPPKREGA | ISDNTKAALK |
| 490 | 500 | 510 | 520 | 530 | 540 |
| AALLKSLRSR | LRDGPSAPLE | APEPRKPVTA | QERQREREEK | RRRRQERAKE | REKRRQERER |
| 550 | 560 | 570 | 580 | 590 | 600 |
| KERGAGTLGG | PSTDPLAGLV | LSDNDRSLLE | RWTRMARPPV | PAPAPAPAPT | PKPSSAQPTS |
| 610 | 620 | 630 | 640 | 650 | 660 |
| PPNGPVSQST | APLQPAGSIP | GPASQPVCPP | PGPVPQPAGP | VPAPLQTAPS | TSLLASQSLV |
| 670 | 680 | 690 | 700 | 710 | 720 |
| PPSGLPGSGA | PEVLPYFPSG | PPPPDPGLTP | QPSTSESPDV | NLVTQQLSKS | QVEDPLPPVF |
| 730 | 740 | 750 | 760 | 770 | 780 |
| SGTPKGSGAG | YGVGFDLEEF | LNQSFDMGVA | DGPQDGQADS | ASLSASLLAD | WLEGHGMNPA |
| 790 | 800 | ||||
| DIESLQREIQ | MDSPMLLSDL | PDLQEP |