P0C7Q4
SS
Gene name |
GNAT3 |
Protein name |
Guanine nucleotide-binding protein G |
Names |
t subunit alpha-3 , Gustducin alpha-3 chain |
Species |
Bos taurus (Bovine) |
KEGG Pathway |
bta:516262 |
EC number |
|
Protein Class |
|
Descriptions
Gαi1 is a G-protein alpha subunit that plays a crucial role in signal transduction pathway initiated by G-protein-coupled receptor (GPCR) activation. G proteins function as transducers downstream of GPCRs in numerous signaling cascades. The alpha subunit contains the guanine nucleotide binding site and alternates between an active (GTP-bound state) and an inactive (GDP-bound state). Activated GPCR induces conformational change in Gαi1, which allows for GDP-to-GTP exchange by binding to the GPCR-binding site within a Ras-like domain, leading to dissociation of the Gαi1 subunit and initiation of downstream signaling.
Autoinhibitory domains (AIDs)
Target domain |
184-348 (Ras-like domain) |
Relief mechanism |
Ligand binding, Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Goricanec D et al. (2016) "Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding", Proceedings of the National Academy of Sciences of the United States of America, 113, E3629-38
- Coleman DE et al. (1999) "Structure of Gialpha1.GppNHp, autoinhibition in a galpha protein-substrate complex", The Journal of biological chemistry, 274, 16669-72
- Lutz S et al. (2007) "Structure of Galphaq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs", Science (New York, N.Y.), 318, 1923-7
Autoinhibited structure
Activated structure
3 structures for P0C7Q4
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 4A4M | X-ray | 330 A | B | 344-354 | PDB |
| 5EN0 | X-ray | 281 A | B | 344-354 | PDB |
| AF-P0C7Q4-F1 | Predicted | AlphaFoldDB |
57 variants for P0C7Q4
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs455789187 | 13 | A>P | No | EVA | |
| rs472401266 | 34 | V>A | No | EVA | |
| rs434536493 | 35 | K>N | No | EVA | |
| rs210187349 | 99 | R>G | No | EVA | |
| rs454825372 | 105 | Q>R | No | EVA | |
| rs465586024 | 155 | Y>C | No | EVA | |
| rs465586024 | 155 | Y>F | No | EVA | |
| rs465586024 | 155 | Y>S | No | EVA | |
| rs435040721 | 160 | D>N | No | EVA | |
| rs457519519 | 163 | A>E | No | EVA | |
| rs474557734 | 167 | Y>* | No | EVA | |
| rs443064358 | 168 | V>I | No | EVA | |
| rs453245449 | 169 | P>Q | No | EVA | |
| rs438903394 | 177 | S>Y | No | EVA | |
| rs482112344 | 179 | V>E | No | EVA | |
| rs444242152 | 181 | T>A | No | EVA | |
| rs460980411 | 181 | T>I | No | EVA | |
| rs481384145 | 182 | T>S | No | EVA | |
| rs446694730 | 183 | G>* | No | EVA | |
| rs466542456 | 183 | G>E | No | EVA | |
| rs477011207 | 184 | I>S | No | EVA | |
| rs445646985 | 185 | I>T | No | EVA | |
| rs469111952 | 186 | E>G | No | EVA | |
| rs467949209 | 188 | Q>H | No | EVA | |
| rs457531702 | 188 | Q>K | No | EVA | |
| rs436693668 | 189 | F>L | No | EVA | |
| rs453555380 | 191 | F>L | No | EVA | |
| rs473674808 | 195 | H>R | No | EVA | |
| rs480743964 | 198 | M>I | No | EVA | |
| rs43385637 | 211 | W>C | No | EVA | |
| rs465076126 | 271 | K>T | No | EVA | |
| rs436791476 | 276 | E>D | No | EVA | |
| rs467485770 | 280 | K>R | No | EVA | |
| rs436039928 | 286 | C>G | No | EVA | |
| rs455876086 | 289 | E>* | No | EVA | |
| rs472806766 | 289 | E>D | No | EVA | |
| rs441564656 | 290 | Y>N | No | EVA | |
| rs458589529 | 298 | D>E | No | EVA | |
| rs438817307 | 298 | D>V | No | EVA | |
| rs444172232 | 302 | Y>H | No | EVA | |
| rs461287818 | 303 | I>S | No | EVA | |
| rs464582516 | 309 | D>Y | No | EVA | |
| rs446481788 | 310 | L>Q | No | EVA | |
| rs460087947 | 311 | N>I | No | EVA | |
| rs469048674 | 312 | L>* | No | EVA | |
| rs445728988 | 312 | L>V | No | EVA | |
| rs437424299 | 319 | I>L | No | EVA | |
| rs450984905 | 319 | I>N | No | EVA | |
| rs468139394 | 320 | Y>C | No | EVA | |
| rs436630549 | 321 | S>P | No | EVA | |
| rs453194058 | 323 | M>I | No | EVA | |
| rs473169603 | 327 | T>P | No | EVA | |
| rs432474225 | 333 | K>E | No | EVA | |
| rs452519795 | 334 | F>L | No | EVA | |
| rs475895066 | 343 | I>L | No | EVA | |
| rs444263348 | 346 | E>D | No | EVA | |
| rs474930862 | 349 | K>E | No | EVA |
No associated diseases with P0C7Q4
No regional properties for P0C7Q4
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for P0C7Q4 | |||
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| heterotrimeric G-protein complex | Any of a family of heterotrimeric GTP-binding and hydrolyzing proteins; they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. Heterotrimeric G-proteins consist of three subunits; the alpha subunit contains the guanine nucleotide binding site and possesses GTPase activity; the beta and gamma subunits are tightly associated and function as a beta-gamma heterodimer; extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G protein-coupled receptors to an effector protein. |
| photoreceptor inner segment | The inner segment of a vertebrate photoreceptor containing mitochondria, ribosomes and membranes where opsin molecules are assembled and passed to be part of the outer segment discs. |
| photoreceptor outer segment | The outer segment of a vertebrate photoreceptor that contains a stack of membrane discs embedded with photoreceptor proteins. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| G protein-coupled receptor binding | Binding to a G protein-coupled receptor. |
| G-protein beta/gamma-subunit complex binding | Binding to a complex of G-protein beta/gamma subunits. |
| GTP binding | Binding to GTP, guanosine triphosphate. |
| GTPase activity | Catalysis of the reaction |
| metal ion binding | Binding to a metal ion. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| adenylate cyclase-modulating G protein-coupled receptor signaling pathway | A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation or inhibition of adenylyl cyclase activity and a subsequent change in the intracellular concentration of cyclic AMP (cAMP). |
| detection of chemical stimulus involved in sensory perception of bitter taste | The series of events required for a bitter taste stimulus to be received and converted to a molecular signal. |
| sensory perception of sweet taste | The series of events required to receive a sweet taste stimulus, convert it to a molecular signal, and recognize and characterize the signal. This is a neurological process. |
58 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P04695 | GNAT1 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P04696 | GNAT2 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P08239 | GNAO1 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P63097 | GNAI1 | Guanine nucleotide-binding protein G | Bos taurus (Bovine) | SS |
| P38408 | GNA14 | Guanine nucleotide-binding protein subunit alpha-14 | Bos taurus (Bovine) | PR |
| P04896 | GNAS | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | Bos taurus (Bovine) | PR |
| P50147 | GNAI2 | Guanine nucleotide-binding protein G | Gallus gallus (Chicken) | SS |
| P50146 | GNAI1 | Guanine nucleotide-binding protein G | Gallus gallus (Chicken) | SS |
| P20353 | Galphai | G protein alpha i subunit | Drosophila melanogaster (Fruit fly) | SS |
| P16378 | Galphao | G protein alpha o subunit | Drosophila melanogaster (Fruit fly) | SS |
| P25157 | cta | Guanine nucleotide-binding protein subunit alpha homolog | Drosophila melanogaster (Fruit fly) | SS |
| P11488 | GNAT1 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P19087 | GNAT2 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P09471 | GNAO1 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| Q03113 | GNA12 | Guanine nucleotide-binding protein subunit alpha-12 | Homo sapiens (Human) | SS |
| P04899 | GNAI2 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| P63096 | GNAI1 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | EV |
| P08754 | GNAI3 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| Q14344 | GNA13 | Guanine nucleotide-binding protein subunit alpha-13 | Homo sapiens (Human) | SS |
| P19086 | GNAZ | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| A8MTJ3 | GNAT3 | Guanine nucleotide-binding protein G | Homo sapiens (Human) | SS |
| Q9DC51 | Gnai3 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P27600 | Gna12 | Guanine nucleotide-binding protein subunit alpha-12 | Mus musculus (Mouse) | SS |
| P20612 | Gnat1 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P27601 | Gna13 | Guanine nucleotide-binding protein subunit alpha-13 | Mus musculus (Mouse) | SS |
| O70443 | Gnaz | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P50149 | Gnat2 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P08752 | Gnai2 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| B2RSH2 | Gnai1 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P18872 | Gnao1 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| Q3V3I2 | Gnat3 | Guanine nucleotide-binding protein G | Mus musculus (Mouse) | SS |
| P93564 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Solanum tuberosum (Potato) | SS |
| Q63210 | Gna12 | Guanine nucleotide-binding protein subunit alpha-12 | Rattus norvegicus (Rat) | SS |
| P19627 | Gnaz | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| P04897 | Gnai2 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| P59215 | Gnao1 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| P08753 | Gnai3 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| Q6Q7Y5 | Gna13 | Guanine nucleotide-binding protein subunit alpha-13 | Rattus norvegicus (Rat) | SS |
| P29348 | Gnat3 | Guanine nucleotide-binding protein G | Rattus norvegicus (Rat) | SS |
| Q0DJ33 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Oryza sativa subsp. japonica (Rice) | SS |
| P51875 | goa-1 | Guanine nucleotide-binding protein G | Caenorhabditis elegans | SS |
| Q18434 | odr-3 | Guanine nucleotide-binding protein alpha-17 subunit | Caenorhabditis elegans | SS |
| Q9BIG4 | gpa-10 | Guanine nucleotide-binding protein alpha-10 subunit | Caenorhabditis elegans | PR |
| P28052 | gpa-3 | Guanine nucleotide-binding protein alpha-3 subunit | Caenorhabditis elegans | SS |
| Q9XTB2 | gpa-13 | Guanine nucleotide-binding protein alpha-13 subunit | Caenorhabditis elegans | SS |
| Q9BIG5 | gpa-4 | Guanine nucleotide-binding protein alpha-4 subunit | Caenorhabditis elegans | SS |
| P28051 | gpa-1 | Guanine nucleotide-binding protein alpha-1 subunit | Caenorhabditis elegans | SS |
| Q9N2V6 | gpa-16 | Guanine nucleotide-binding protein alpha-16 subunit | Caenorhabditis elegans | SS |
| P91907 | gpa-15 | Guanine nucleotide-binding protein alpha-15 subunit | Caenorhabditis elegans | SS |
| Q20907 | gpa-8 | Guanine nucleotide-binding protein alpha-8 subunit | Caenorhabditis elegans | PR |
| Q21917 | gpa-7 | Guanine nucleotide-binding protein alpha-7 subunit | Caenorhabditis elegans | SS |
| Q93743 | gpa-6 | Guanine nucleotide-binding protein alpha-6 subunit | Caenorhabditis elegans | SS |
| O76584 | gpa-11 | Guanine nucleotide-binding protein alpha-11 subunit | Caenorhabditis elegans | SS |
| P49084 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Glycine max (Soybean) (Glycine hispida) | SS |
| P93163 | GPA2 | Guanine nucleotide-binding protein alpha-2 subunit | Glycine max (Soybean) (Glycine hispida) | SS |
| O80462 | XLG1 | Extra-large guanine nucleotide-binding protein 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| P18064 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P26981 | GPA1 | Guanine nucleotide-binding protein alpha-1 subunit | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MGIGISSESK | ESAKRSKELE | KKLQEDAERD | ARTVKLLLLG | AGESGKSTIV | KQMKIIHKNG |
| 70 | 80 | 90 | 100 | 110 | 120 |
| YSEQECMEFK | AVIYSNTLQS | ILAIVKAMAT | LEIDYVNPRS | AEDQQQLCAM | ANTLEDGSMT |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PELAEIIKRL | WRDPGVQACF | ERASEYQLND | SAAYYLNDLD | RIAAPGYVPN | EQDVLHSRVK |
| 190 | 200 | 210 | 220 | 230 | 240 |
| TTGIIETQFS | FKDLHFRMFD | VGGQRSERKK | WIHCFEGVTC | IIFCAALSAY | DMVLVEDEEV |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NRMHESLHLF | NSICNHKYFA | TTSIVLFLNK | KDLFQEKVTK | VHLSICFPEY | TGPNTFEDAG |
| 310 | 320 | 330 | 340 | 350 | |
| NYIKNQFLDL | NLKKEDKEIY | SHMTCATDTQ | NVKFVFDAVT | DIIIKENLKD | CGLF |