P0C605

SS
Gene name
Prkg1 (Prkg1b, Prkgr1a, Prkgr1b)
Protein name
cGMP-dependent protein kinase 1
Names
cGK 1 , cGK1 , EC 2.7.11.12 , cGMP-dependent protein kinase I , cGKI
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:19091
EC number
2.7.11.12: Protein-serine/threonine kinases
Protein Class

Descriptions

PKG is a major intracellular receptor of cGMP and modulates diverse cellular signalings. The activity of PKG1B is regulated by its N-terminal regulatory (R) domain, which includes a leucine zipper (LZ) domain, a linker region with an autoinhibitory (AI) sequence, and two cyclic nucleotide-binding (CNB-A and -B) domains. In the autoinhibition of PKG1B, the AI sequence from R domain binds to the catalytic cleft of the C domain, blocking substrate access and inhibiting the enzymatic activity. This autoinhibition is alleviated by the binding of cyclic guanosine monophosphate (cGMP) to the CNB domains, which destabilizes the autoinhibited state and allows the active kinase to phosphorylate downstream substrate.

Autoinhibitory domains (AIDs)

85-89 (AI sequence) SS

Target domain

355-671 (C-terminal catalytic domain)

Relief mechanism

Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

0 structures for P0C605

Entry ID Method Resolution Chain Position Source
No available structures

12 variants for P0C605

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3389547171 8 F>L No EVA
rs3389549834 51 S>A No EVA
rs3409067244 145 D>E No EVA
rs3389527276 162 P>S No EVA
rs1131995958 185 V>G No EVA
rs3389536458 189 L>F No EVA
rs3389527342 226 P>H No EVA
rs3389539012 263 K>N No EVA
rs3389547170 491 I>T No EVA
rs1134388234 501 V>A No EVA
rs3389442441 518 F>L No EVA
rs3389535666 618 W>* No EVA

No associated diseases with P0C605

No regional properties for P0C605

Type Name Position InterPro Accession
No domain, repeats, and functional sites for P0C605

Functions

Description
EC Number 2.7.11.12 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Colocalized with TRPC7 in the plasma membrane
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
acrosomal vesicle A structure in the head of a spermatozoon that contains acid hydrolases, and is concerned with the breakdown of the outer membrane of the ovum during fertilization. It lies just beneath the plasma membrane and is derived from the lysosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
sarcolemma The outer membrane of a muscle cell, consisting of the plasma membrane, a covering basement membrane (about 100 nm thick and sometimes common to more than one fiber), and the associated loose network of collagen fibers.

8 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calcium channel regulator activity Modulates the activity of a calcium channel.
cGMP binding Binding to cGMP, the nucleotide cyclic GMP (guanosine 3',5'-cyclophosphate).
cGMP-dependent protein kinase activity cGMP dependent catalysis of the reaction
identical protein binding Binding to an identical protein or proteins.
mitogen-activated protein kinase p38 binding Binding to mitogen-activated protein kinase p38, an enzyme that catalyzes the transfer of phosphate from ATP to hydroxyl side chains on proteins in response to mitogen activation.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions

21 GO annotations of biological process

Name Definition
cell growth involved in cardiac muscle cell development The growth of a cardiac muscle cell, where growth contributes to the progression of the cell over time from its initial formation to its mature state.
cerebellum development The process whose specific outcome is the progression of the cerebellum over time, from its formation to the mature structure. The cerebellum is the portion of the brain in the back of the head between the cerebrum and the pons. In mice, the cerebellum controls balance for walking and standing, modulates the force and range of movement and is involved in the learning of motor skills.
cGMP-mediated signaling Any intracellular signal transduction in which the signal is passed on within the cell via cyclic GMP (cGMP). Includes production of cGMP, and downstream effectors that further transmit the signal within the cell.
collateral sprouting The process in which outgrowths develop from the shafts of existing axons.
dendrite development The process whose specific outcome is the progression of the dendrite over time, from its formation to the mature structure.
forebrain development The process whose specific outcome is the progression of the forebrain over time, from its formation to the mature structure. The forebrain is the anterior of the three primary divisions of the developing chordate brain or the corresponding part of the adult brain (in vertebrates, includes especially the cerebral hemispheres, the thalamus, and the hypothalamus and especially in higher vertebrates is the main control center for sensory and associative information processing, visceral functions, and voluntary motor functions).
negative regulation of glutamate secretion Any process that stops, prevents, or reduces the frequency, rate or extent of the controlled release of glutamate.
negative regulation of inositol phosphate biosynthetic process Any process that decreases the rate, frequency or extent of inositol phosphate biosynthesis. Inositol phosphate biosynthetic processes are the chemical reactions and pathways resulting in the formation of an inositol phosphate, 1,2,3,4,5,6-cyclohexanehexol, with one or more phosphate groups attached.
negative regulation of platelet aggregation Any process that decreases the rate, frequency or extent of platelet aggregation. Platelet aggregation is the adhesion of one platelet to one or more other platelets via adhesion molecules.
negative regulation of smooth muscle contraction Any process that stops, prevents, or reduces the frequency, rate or extent of smooth muscle contraction.
negative regulation of vascular associated smooth muscle cell migration Any process that stops, prevents or reduces the frequency, rate or extent of vascular associated smooth muscle cell migration.
negative regulation of vascular associated smooth muscle cell proliferation Any process that stops, prevents or reduces the frequency, rate or extent of vascular smooth muscle cell proliferation.
neuron migration The characteristic movement of an immature neuron from germinal zones to specific positions where they will reside as they mature.
positive regulation of circadian rhythm Any process that activates or increases the frequency, rate or extent of a circadian rhythm behavior.
positive regulation of cytosolic calcium ion concentration Any process that increases the concentration of calcium ions in the cytosol.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of GTPase activity Any process that modulates the rate of GTP hydrolysis by a GTPase.
regulation of testosterone biosynthetic process Any process that modulates the frequency, rate or extent of testosterone biosynthetic process.
relaxation of vascular associated smooth muscle A negative regulation of smooth muscle contraction resulting in relaxation of vascular smooth muscle. The relaxation is mediated by a decrease in the phosphorylation state of myosin light chain. This can be achieved by removal of calcium from the cytoplasm to the sarcoplasmic reticulum lumen through the action of Ca2+ ATPases leading to a decrease myosin light chain kinase activity, and through calcium-independent pathways leading to a increase in myosin light chain phosphatase activity.
signal transduction The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
spermatid development The process whose specific outcome is the progression of a spermatid over time, from its formation to the mature structure.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P00516 PRKG1 cGMP-dependent protein kinase 1 Bos taurus (Bovine) SS
Q03043 for cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B Drosophila melanogaster (Fruit fly) SS
Q13237 PRKG2 cGMP-dependent protein kinase 2 Homo sapiens (Human) PR
Q13976 PRKG1 cGMP-dependent protein kinase 1 Homo sapiens (Human) EV
P05132 Prkaca cAMP-dependent protein kinase catalytic subunit alpha Mus musculus (Mouse) PR
Q922R0 Prkx cAMP-dependent protein kinase catalytic subunit PRKX Mus musculus (Mouse) PR
Q61410 Prkg2 cGMP-dependent protein kinase 2 Mus musculus (Mouse) PR
Q64595 Prkg2 cGMP-dependent protein kinase 2 Rattus norvegicus (Rat) PR
O76360 egl-4 cGMP-dependent protein kinase egl-4 Caenorhabditis elegans PR
10 20 30 40 50 60
MSELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP STHIGPRTTR
70 80 90 100 110 120
AQGISAEPQT YRSFHDLRQA FRKFTKSERS KDLIKEAILD NDFMKNLELS QIQEIVDCMY
130 140 150 160 170 180
PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV TKEGVKLCTM GPGKVFGELA ILYNCTRTAT
190 200 210 220 230 240
VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT EYMEFLKSVP TFQSLPDEIL SKLADVLEET
250 260 270 280 290 300
HYENGEYIIR QGARGDTFFI ISKGQVNVTR EDSPSEDPVF LRTLGKGDWF GEKALQGEDV
310 320 330 340 350 360
RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA FFANLKLSDF
370 380 390 400 410 420
NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF
430 440 450 460 470 480
IVRLYRTFKD SKYLYMLMEA CLGGELWTIL RDRGSFEDST TRFYTACVVE AFAYLHSKGI
490 500 510 520 530 540
IYRDLKPENL ILDHRGYAKL VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD
550 560 570 580 590 600
YWSLGILMYE LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS
610 620 630 640 650 660
ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD SFPEDSDEPP
670
PDDNSGWDID F