P06782
Gene name |
SNF1 |
Protein name |
Carbon catabolite-derepressing protein kinase |
Names |
Sucrose nonfermentating protein 1 |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YDR477W |
EC number |
2.7.11.1: Protein-serine/threonine kinases |
Protein Class |
MAP/MICROTUBULE AFFINITY-REGULATING KINASE (PTHR24346) |
Descriptions
SNF1, a member of the SNF1/AMPK family, is required for the adaptation of yeast cells to glucose limitation and for growth on carbon sources that are less preferred than glucose. SNF1 regulates transcription of a large set of genes, modifies the activity of metabolic enzymes, and controls various nutrient-responsive cellular developmental processes. In yeast, the SNF1 kinase is complexed with the SNF4 protein, which is required for SNF1 kinase activity. In glucose-grown cells, the SNF1 kinase complex exists predominantly in an inactive autoinhibited conformation in which the catalytic domain of SNF1 binds to the regulatory domain of the protein. When glucose is limiting, the catalytic domain is released and the activating subunit SNF4 binds to the regulatory domain, which leads to an active, open conformation of the complex. Activation requires phosphorylation and an essential, conserved threonine in the activation loop of SNF1, which is phosphorylated during the activation of other kinases.
Autoinhibitory domains (AIDs)
Target domain |
55-306 (Protein kinase domain) |
Relief mechanism |
Partner binding |
Assay |
Deletion assay, Mutagenesis experiment, Structural analysis |
Target domain |
55-306 (Protein kinase domain) |
Relief mechanism |
Ligand binding |
Assay |
Structural analysis |
Accessory elements
194-216 (Activation loop from InterPro)
Target domain |
55-306 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
- Jiang R et al. (1997) "The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex", Molecular and cellular biology, 17, 2099-106
- Jiang R et al. (1996) "Glucose regulates protein interactions within the yeast SNF1 protein kinase complex", Genes & development, 10, 3105-15
- Chen L et al. (2009) "Structural insight into the autoinhibition mechanism of AMP-activated protein kinase", Nature, 459, 1146-9
- Amodeo GA et al. (2007) "Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1", Nature, 449, 492-5
- Sanz P et al. (2000) "Regulatory interactions between the Reg1-Glc7 protein phosphatase and the Snf1 protein kinase", Molecular and cellular biology, 20, 1321-8
- Pufall MA et al. (2002) "Autoinhibitory domains: modular effectors of cellular regulation", Annual review of cell and developmental biology, 18, 421-62
- Hedbacker K et al. (2008) "SNF1/AMPK pathways in yeast", Frontiers in bioscience : a journal and virtual library, 13, 2408-20
- Ludin K et al. (1998) "Glucose-regulated interaction of a regulatory subunit of protein phosphatase 1 with the Snf1 protein kinase in Saccharomyces cerevisiae", Proceedings of the National Academy of Sciences of the United States of America, 95, 6245-50
Autoinhibited structure
Activated structure
9 structures for P06782
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 2FH9 | X-ray | 280 A | A | 46-319 | PDB |
| 2QLV | X-ray | 260 A | A/D | 460-630 | PDB |
| 3DAE | X-ray | 290 A | A/B | 41-315 | PDB |
| 3HYH | X-ray | 220 A | A/B | 41-315 | PDB |
| 3MN3 | X-ray | 238 A | A | 50-320 | PDB |
| 3T4N | X-ray | 230 A | A | 457-633 | PDB |
| 3TDH | X-ray | 230 A | A | 457-633 | PDB |
| 3TE5 | X-ray | 250 A | A | 457-633 | PDB |
| AF-P06782-F1 | Predicted | AlphaFoldDB |
3 variants for P06782
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s04-1412440 | 26 | H>N | No | SGRP | |
| s04-1413845 | 494 | S>C | No | SGRP | |
| s04-1414031 | 556 | T>R | No | SGRP |
No associated diseases with P06782
5 regional properties for P06782
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 55 - 306 | IPR000719 |
| active_site | Serine/threonine-protein kinase, active site | 173 - 185 | IPR008271 |
| domain | Carbon catabolite-derepressing protein kinase, ubiquitin-associated domain | 344 - 395 | IPR013896 |
| binding_site | Protein kinase, ATP binding site | 61 - 84 | IPR017441 |
| domain | AMPK, C-terminal adenylate sensor domain | 504 - 626 | IPR032270 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.1 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | PTHR24346 | MAP/MICROTUBULE AFFINITY-REGULATING KINASE |
| PANTHER Subfamily | PTHR24346:SF82 | SERINE_THREONINE-PROTEIN KINASE MARK-A-RELATED |
| PANTHER Protein Class |
non-receptor serine/threonine protein kinase
protein modifying enzyme |
|
| PANTHER Pathway Category |
p53 pathway by glucose deprivation AMPK |
|
8 GO annotations of cellular component
| Name | Definition |
|---|---|
| cellular bud neck septin ring | A ring-shaped structure that forms at the site of cytokinesis in the bud neck of a budding cell; composed of members of the conserved family of filament forming proteins called septins as well as septin-associated proteins. In S. cerevisiae, this structure forms at the time of bud emergence and the septins show a high rate of exchange. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| mitochondrion | A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
| nuclear envelope lumen | The region between the two lipid bilayers of the nuclear envelope; 20-40 nm wide. |
| nuclear membrane | Either of the lipid bilayers that surround the nucleus and form the nuclear envelope; excludes the intermembrane space. |
| nucleotide-activated protein kinase complex | A protein complex that possesses nucleotide-dependent protein kinase activity. The nucleotide can be AMP (in S. pombe and human) or ADP (in S. cerevisiae). |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| vacuolar membrane | The lipid bilayer surrounding the vacuole and separating its contents from the cytoplasm of the cell. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| AMP-activated protein kinase activity | Catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein. This reaction requires the presence of AMP. |
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| guanyl-nucleotide exchange factor activity | Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions. |
| identical protein binding | Binding to an identical protein or proteins. |
| protein kinase activity | Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP. |
| protein serine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate. |
| protein serine/threonine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate. |
| protein serine/threonine/tyrosine kinase activity | Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate. |
19 GO annotations of biological process
| Name | Definition |
|---|---|
| carbohydrate metabolic process | The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. |
| cell adhesion | The attachment of a cell, either to another cell or to an underlying substrate such as the extracellular matrix, via cell adhesion molecules. |
| cellular response to nitrogen starvation | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nitrogen. |
| establishment of mitotic spindle orientation | A cell cycle process that sets the alignment of mitotic spindle relative to other cellular structures. |
| filamentous growth | The process in which a multicellular organism, a unicellular organism or a group of unicellular organisms grow in a threadlike, filamentous shape. |
| fungal-type cell wall assembly | The aggregation, arrangement and bonding together of a set of components to form a fungal-type cell wall. |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| invasive growth in response to glucose limitation | A growth pattern exhibited by budding haploid cells under certain growth conditions, in which cells retain the typical axial budding pattern of haploids, but become elongated and fail to separate after division; during growth on a solid substrate, this results in penetration of cells into the agar medium. An example of this process is found in Saccharomyces cerevisiae. |
| negative regulation of translation | Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA. |
| positive regulation of filamentous growth of a population of unicellular organisms in response to starvation | Any process that activates or increases the frequency, rate or extent of filamentous growth of a population of unicellular organisms in response to starvation. |
| positive regulation of gluconeogenesis | Any process that activates or increases the frequency, rate or extent of gluconeogenesis. |
| positive regulation of macroautophagy | Any process, such as recognition of nutrient depletion, that activates or increases the rate of macroautophagy to bring cytosolic macromolecules to the vacuole/lysosome for degradation. |
| positive regulation of pseudohyphal growth | Any process that activates or increases the frequency, rate or extent of pseudohyphal growth. |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
| regulation of cellular response to glucose starvation | Any process that modulates the frequency, rate or extent of cellular response to glucose starvation. |
| regulation of invasive growth in response to glucose limitation | Any process that modulates the frequency, rate or extent of invasive growth in response to glucose limitation. |
| response to endoplasmic reticulum stress | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stress acting at the endoplasmic reticulum. ER stress usually results from the accumulation of unfolded or misfolded proteins in the ER lumen. |
| response to unfolded protein | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an unfolded protein stimulus. |
| single-species surface biofilm formation | A process in which microorganisms produce an extracellular matrix and form multicellular aggregates at an air-liquid interface. |
33 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P36004 | KKQ8 | Probable serine/threonine-protein kinase KKQ8 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P28708 | PRR1 | Serine/threonine-protein kinase PRR1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P47116 | PTK2 | Serine/threonine-protein kinase PTK2/STK2 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P57059 | SIK1 | Serine/threonine-protein kinase SIK1 | Homo sapiens (Human) | PR |
| O60285 | NUAK1 | NUAK family SNF1-like kinase 1 | Homo sapiens (Human) | PR |
| P54646 | PRKAA2 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | Homo sapiens (Human) | EV |
| Q13131 | PRKAA1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Homo sapiens (Human) | EV |
| Q8C0X8 | Sperm motility kinase X | Mus musculus (Mouse) | PR | |
| A0AUV4 | Gm7168 | Sperm motility kinase Y | Mus musculus (Mouse) | PR |
| Q8C0N0 | Gm4922 | Sperm motility kinase Z | Mus musculus (Mouse) | PR |
| Q641K5 | Nuak1 | NUAK family SNF1-like kinase 1 | Mus musculus (Mouse) | PR |
| Q8BRK8 | Prkaa2 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | Mus musculus (Mouse) | SS |
| Q5EG47 | Prkaa1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Mus musculus (Mouse) | SS |
| A2KF29 | Smoktcr | Sperm motility kinase Tcr mutant form | Mus musculus (Mouse) | PR |
| Q28948 | PRKAA2 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | Sus scrofa (Pig) | SS |
| Q09136 | PRKAA1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Sus scrofa (Pig) | SS |
| Q09137 | Prkaa2 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | Rattus norvegicus (Rat) | EV |
| P54645 | Prkaa1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Rattus norvegicus (Rat) | EV |
| Q8LIG4 | CIPK3 | CBL-interacting protein kinase 3 | Oryza sativa subsp japonica (Rice) | PR |
| Q6ZLP5 | CIPK23 | CBL-interacting protein kinase 23 | Oryza sativa subsp japonica (Rice) | PR |
| Q852Q1 | OSK4 | Serine/threonine protein kinase OSK4 | Oryza sativa subsp. japonica (Rice) | SS |
| Q852Q2 | OSK1 | Serine/threonine protein kinase OSK1 | Oryza sativa subsp. japonica (Rice) | SS |
| Q2RAX3 | CIPK33 | CBL-interacting protein kinase 33 | Oryza sativa subsp japonica (Rice) | PR |
| Q2QY53 | CIPK32 | CBL-interacting protein kinase 32 | Oryza sativa subsp japonica (Rice) | PR |
| Q21017 | kin-29 | Serine/threonine-protein kinase kin-29 | Caenorhabditis elegans | SS |
| P45894 | aak-1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Caenorhabditis elegans | SS |
| Q95ZQ4 | aak-2 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | Caenorhabditis elegans | SS |
| Q94CG0 | CIPK21 | CBL-interacting serine/threonine-protein kinase 21 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q93VD3 | CIPK23 | CBL-interacting serine/threonine-protein kinase 23 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LDI3 | CIPK24 | CBL-interacting serine/threonine-protein kinase 24 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9FLZ3 | KIN12 | SNF1-related protein kinase catalytic subunit alpha KIN12 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P92958 | KIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38997 | KIN10 | SNF1-related protein kinase catalytic subunit alpha KIN10 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSSNNNTNTA | PANANSSHHH | HHHHHHHHHH | GHGGSNSTLN | NPKSSLADGA | HIGNYQIVKT |
| 70 | 80 | 90 | 100 | 110 | 120 |
| LGEGSFGKVK | LAYHTTTGQK | VALKIINKKV | LAKSDMQGRI | EREISYLRLL | RHPHIIKLYD |
| 130 | 140 | 150 | 160 | 170 | 180 |
| VIKSKDEIIM | VIEYAGNELF | DYIVQRDKMS | EQEARRFFQQ | IISAVEYCHR | HKIVHRDLKP |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ENLLLDEHLN | VKIADFGLSN | IMTDGNFLKT | SCGSPNYAAP | EVISGKLYAG | PEVDVWSCGV |
| 250 | 260 | 270 | 280 | 290 | 300 |
| ILYVMLCRRL | PFDDESIPVL | FKNISNGVYT | LPKFLSPGAA | GLIKRMLIVN | PLNRISIHEI |
| 310 | 320 | 330 | 340 | 350 | 360 |
| MQDDWFKVDL | PEYLLPPDLK | PHPEEENENN | DSKKDGSSPD | NDEIDDNLVN | ILSSTMGYEK |
| 370 | 380 | 390 | 400 | 410 | 420 |
| DEIYESLESS | EDTPAFNEIR | DAYMLIKENK | SLIKDMKANK | SVSDELDTFL | SQSPPTFQQQ |
| 430 | 440 | 450 | 460 | 470 | 480 |
| SKSHQKSQVD | HETAKQHARR | MASAITQQRT | YHQSPFMDQY | KEEDSTVSIL | PTSLPQIHRA |
| 490 | 500 | 510 | 520 | 530 | 540 |
| NMLAQGSPAA | SKISPLVTKK | SKTRWHFGIR | SRSYPLDVMG | EIYIALKNLG | AEWAKPSEED |
| 550 | 560 | 570 | 580 | 590 | 600 |
| LWTIKLRWKY | DIGNKTNTNE | KIPDLMKMVI | QLFQIETNNY | LVDFKFDGWE | SSYGDDTTVS |
| 610 | 620 | 630 | |||
| NISEDEMSTF | SAYPFLHLTT | KLIMELAVNS | QSN |