P05625
Gene name |
RAF1 (MIL) |
Protein name |
RAF proto-oncogene serine/threonine-protein kinase |
Names |
PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, C-MIL, C-RAF, MIL proto-oncogene serine/threonine-protein kinase, RAF-1 |
Species |
Gallus gallus (Chicken) |
KEGG Pathway |
gga:396245 |
EC number |
2.7.11.1: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
(Annotation based on sequence homology with P04049)
Raf proteins are Ras-regulated serine/threonine protein kinases that control the activation of the ERK/MARK cascade, and consists of three isoforms, A-Raf, B-Raf, and Raf-1 (C-Raf). When the catalytic domain of Raf-1 is expressed alone, it exhibits a constitutive activity. Raf-1 is regulated by an N-terminal autoinhibitory domain including Ras binding domain (RBD) and cysteine-rich domain (CRD). The autoinhibitory region blocks the catalytic kinase domain and the autoinhibition is interrupted by the interaction with active PAK1 or Src.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
485-510 (Activation loop from InterPro)
Target domain |
349-609 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
1 structures for P05625
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P05625-F1 | Predicted | AlphaFoldDB |
5 variants for P05625
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs314452077 | 59 | R>* | No | Ensembl | |
| rs733498564 | 220 | S>A | No | Ensembl | |
| rs741363668 | 421 | T>P | No | Ensembl | |
| rs318093585 | 458 | Y>C | No | Ensembl | |
| rs736494419 | 504 | Q>H | No | Ensembl |
No associated diseases with P05625
9 regional properties for P05625
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 349 - 609 | IPR000719 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 351 - 605 | IPR001245 |
| domain | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | 138 - 186 | IPR002219 |
| domain | Raf-like Ras-binding | 56 - 131 | IPR003116 |
| active_site | Serine/threonine-protein kinase, active site | 464 - 476 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 355 - 375 | IPR017441 |
| domain | Diacylglycerol/phorbol-ester binding | 136 - 150 | IPR020454-1 |
| domain | Diacylglycerol/phorbol-ester binding | 152 - 172 | IPR020454-2 |
| domain | Diacylglycerol/phorbol-ester binding | 173 - 185 | IPR020454-3 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.1 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| mitochondrion | A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| MAP kinase kinase kinase activity | Catalysis of the phosphorylation and activation of a MAP kinase kinase; each MAP kinase kinase can be phosphorylated by any of several MAP kinase kinase kinases. |
| metal ion binding | Binding to a metal ion. |
| protein serine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate. |
| protein serine/threonine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate. |
2 GO annotations of biological process
| Name | Definition |
|---|---|
| MAPK cascade | An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell. |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
15 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A7E3S4 | RAF1 | RAF proto-oncogene serine/threonine-protein kinase | Bos taurus (Bovine) | SS |
| Q04982 | BRAF | Serine/threonine-protein kinase B-raf | Gallus gallus (Chicken) | SS |
| P11346 | Raf | Raf homolog serine/threonine-protein kinase Raf | Drosophila melanogaster (Fruit fly) | PR |
| P15056 | BRAF | Serine/threonine-protein kinase B-raf | Homo sapiens (Human) | EV |
| P04049 | RAF1 | RAF proto-oncogene serine/threonine-protein kinase | Homo sapiens (Human) | EV |
| P10398 | ARAF | Serine/threonine-protein kinase A-Raf | Homo sapiens (Human) | PR |
| P04627 | Araf | Serine/threonine-protein kinase A-Raf | Mus musculus (Mouse) | PR |
| P28028 | Braf | Serine/threonine-protein kinase B-raf | Mus musculus (Mouse) | SS |
| Q99N57 | Raf1 | RAF proto-oncogene serine/threonine-protein kinase | Mus musculus (Mouse) | SS |
| O19004 | ARAF | Serine/threonine-protein kinase A-Raf | Sus scrofa (Pig) | PR |
| P11345 | Raf1 | RAF proto-oncogene serine/threonine-protein kinase | Rattus norvegicus (Rat) | SS |
| P14056 | Araf | Serine/threonine-protein kinase A-Raf | Rattus norvegicus (Rat) | PR |
| Q07292 | lin-45 | Raf homolog serine/threonine-protein kinase | Caenorhabditis elegans | PR |
| Q05609 | CTR1 | Serine/threonine-protein kinase CTR1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9FPR3 | EDR1 | Serine/threonine-protein kinase EDR1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MEHIQGAWKT | ISNGFGLKDS | VFDGPNCISP | TIVQQFGYQR | RASDDGKISD | TSKTSNTIRV |
| 70 | 80 | 90 | 100 | 110 | 120 |
| FLPNKQRTVV | NVRNGMTLHD | CLMKALKVRG | LQPECCAVFR | LVTEPKGKKV | RLDWNTDAAS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LIGEELQVDF | LDHVPLTTHN | FARKTFLKLA | FCDICQKFLL | NGFRCQTCGY | KFHEHCSTKV |
| 190 | 200 | 210 | 220 | 230 | 240 |
| PTMCVDWSNI | RQLLLFPNSN | ISDSGVPALP | PLTMRRMRES | VSRIPVSSQH | RYSTPHVFTF |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NTSNPSSEGT | LSQRQRSTST | PNVHMVSTTM | PVDSRIIEDA | IRNHSESASP | SALSGSPNNM |
| 310 | 320 | 330 | 340 | 350 | 360 |
| SPTGWSQPKT | PVPAQRERAP | GTNTQEKNKI | RPRGQRDSSY | YWEIEASEVM | LSTRIGSGSF |
| 370 | 380 | 390 | 400 | 410 | 420 |
| GTVYKGKWHG | DVAVKILKVV | DPTPEQFQAF | RNEVAVLRKT | RHVNILLFMG | YMTKDNLAIV |
| 430 | 440 | 450 | 460 | 470 | 480 |
| TQWCEGSSLY | KHLHVQETKF | QMFQLIDIAR | QTAQGMDYLH | AKNIIHRDMK | SNNIFLHEGL |
| 490 | 500 | 510 | 520 | 530 | 540 |
| TVKIGDFGLA | TVKSRWSGSQ | QVEQPTGSIL | WMAPEVIRMQ | DSNPFSFQSD | VYSYGIVLYE |
| 550 | 560 | 570 | 580 | 590 | 600 |
| LMTGELPYSH | INNRDQIIFM | VGRGYASPDL | SKLYKNCPKA | MKRLVADCLK | KVREERPLFP |
| 610 | 620 | 630 | 640 | ||
| QILSSIELLQ | HSLPKINRSA | SEPSLHRASH | TEDINSCTLT | STRLPVF |