Descriptions
PKA is a major intracellular receptor of cAMP and modulates diverse cellular signalings. The N-terminal regulatory domain contains a pseudosubstrate sequence that may interact with the catalytic kinase domain.
Autoinhibitory domains (AIDs)
Target domain |
81-155 (Cyclic nucleotide-binding domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
References
- Scott JD (1991) "Cyclic nucleotide-dependent protein kinases", Pharmacology & therapeutics, 50, 123-45
- Diskar M et al. (2007) "Molecular basis for isoform-specific autoregulation of protein kinase A", Cellular signalling, 19, 2024-34
- Diller TC et al. (2000) "Type II beta regulatory subunit of cAMP-dependent protein kinase: purification strategies to optimize crystallization", Protein expression and purification, 20, 357-64
Autoinhibited structure
Activated structure
1 structures for P05207
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P05207-F1 | Predicted | AlphaFoldDB |
No variants for P05207
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P05207 | |||||
No associated diseases with P05207
1 regional properties for P05207
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Cyclic nucleotide-binding domain | 81 - 155 | IPR000595 |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cAMP-dependent protein kinase complex | An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. Inactive forms of the enzyme have two regulatory chains and two catalytic chains; activation by cAMP produces two active catalytic monomers and a regulatory dimer. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| germinal vesicle | The enlarged, fluid filled nucleus of a primary oocyte, the development of which is suspended in prophase I of the first meiotic division between embryohood and sexual maturity. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| cAMP binding | Binding to cAMP, the nucleotide cyclic AMP (adenosine 3',5'-cyclophosphate). |
| cAMP-dependent protein kinase inhibitor activity | Binds to and stops, prevents or reduces the activity of a cAMP-dependent protein kinase. |
| protein kinase A catalytic subunit binding | Binding to one or both of the catalytic subunits of protein kinase A. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| negative regulation of cAMP-dependent protein kinase activity | Any process that stops, prevents or reduces the frequency, rate or extent of cAMP-dependent protein kinase activity. |
| positive regulation of meiotic cell cycle process involved in oocyte maturation | Any process that activates or increases the frequency, rate or extent of meiotic cell cycle process involved in oocyte maturation. |
| regulation of meiotic cell cycle process involved in oocyte maturation | Any process that modulates the frequency, rate or extent of meiotic cell cycle process involved in oocyte maturation. |
22 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P00514 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Bos taurus (Bovine) | EV |
| P00515 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Bos taurus (Bovine) | EV |
| P31322 | PRKAR2B | cAMP-dependent protein kinase type II-beta regulatory subunit | Bos taurus (Bovine) | EV |
| Q5ZM91 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Gallus gallus (Chicken) | SS |
| P81900 | Pka-R2 | cAMP-dependent protein kinase type II regulatory subunit | Drosophila melanogaster (Fruit fly) | PR |
| P16905 | Pka-R1 | cAMP-dependent protein kinase type I regulatory subunit | Drosophila melanogaster (Fruit fly) | PR |
| P13861 | PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | Homo sapiens (Human) | PR |
| P31323 | PRKAR2B | cAMP-dependent protein kinase type II-beta regulatory subunit | Homo sapiens (Human) | PR |
| Q96M20 | CNBD2 | Cyclic nucleotide-binding domain-containing protein 2 | Homo sapiens (Human) | PR |
| P10644 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Homo sapiens (Human) | EV |
| P31321 | PRKAR1B | cAMP-dependent protein kinase type I-beta regulatory subunit | Homo sapiens (Human) | EV |
| P12849 | Prkar1b | cAMP-dependent protein kinase type I-beta regulatory subunit | Mus musculus (Mouse) | PR |
| P12367 | Prkar2a | cAMP-dependent protein kinase type II-alpha regulatory subunit | Mus musculus (Mouse) | PR |
| P31324 | Prkar2b | cAMP-dependent protein kinase type II-beta regulatory subunit | Mus musculus (Mouse) | PR |
| Q9D5U8 | Cnbd2 | Cyclic nucleotide-binding domain-containing protein 2 | Mus musculus (Mouse) | PR |
| Q9DBC7 | Prkar1a | cAMP-dependent protein kinase type I-alpha regulatory subunit [Cleaved into: cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed] | Mus musculus (Mouse) | SS |
| P07802 | PRKAR1A | cAMP-dependent protein kinase type I-alpha regulatory subunit | Sus scrofa (Pig) | SS |
| P12369 | Prkar2b | cAMP-dependent protein kinase type II-beta regulatory subunit | Rattus norvegicus (Rat) | PR |
| P12368 | Prkar2a | cAMP-dependent protein kinase type II-alpha regulatory subunit | Rattus norvegicus (Rat) | PR |
| P81377 | Prkar1b | cAMP-dependent protein kinase type I-beta regulatory subunit | Rattus norvegicus (Rat) | PR |
| P09456 | Prkar1a | cAMP-dependent protein kinase type I-alpha regulatory subunit | Rattus norvegicus (Rat) | SS |
| P30625 | kin-2 | cAMP-dependent protein kinase regulatory subunit | Caenorhabditis elegans | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| SGSQDLEPSS | GLVTDAIADS | ESEDDEDLDV | PIPSRFDRRV | SVCAETYNPD | EEEEDTDPRV |
| 70 | 80 | 90 | 100 | 110 | 120 |
| IHPKTDQQRC | RLQEACKDIL | LFKNLDQEQL | SQVLDAMFER | TVKVDEHVID | QRDDGDNFYV |
| 130 | 140 | 150 | |||
| IERGTYDILV | TKDNQTRSVG | QYDNHGSFGE | LALMY |