P05132

PR
Gene name
Prkaca (Pkaca)
Protein name
cAMP-dependent protein kinase catalytic subunit alpha
Names
PKA C-alpha
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:18747
EC number
2.7.11.11: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-68 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

184-204 (Activation loop from InterPro)

Target domain

44-298 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

82 structures for P05132

Entry ID Method Resolution Chain Position Source
1APM X-ray 200 A E 2-351 PDB
1ATP X-ray 220 A E 2-351 PDB
1BKX X-ray 260 A A 2-351 PDB
1BX6 X-ray 210 A A 2-351 PDB
1FMO X-ray 220 A E 2-351 PDB
1J3H X-ray 290 A A/B 2-351 PDB
1JBP X-ray 220 A E 2-351 PDB
1JLU X-ray 225 A E 2-351 PDB
1L3R X-ray 200 A E 2-351 PDB
1RDQ X-ray 126 A E 2-351 PDB
1RE8 X-ray 210 A A 2-351 PDB
1REJ X-ray 220 A A 2-351 PDB
1REK X-ray 230 A A 2-351 PDB
1SYK X-ray 280 A A/B 2-351 PDB
2CPK X-ray 270 A E 2-351 PDB
2ERZ X-ray 220 A E 1-351 PDB
2QCS X-ray 220 A A 2-351 PDB
2QUR X-ray 250 A A 2-351 PDB
2QVS X-ray 250 A E 2-351 PDB
3FHI X-ray 200 A A 2-351 PDB
3FJQ X-ray 160 A E 2-351 PDB
3IDB X-ray 162 A A 2-351 PDB
3IDC X-ray 270 A A 2-351 PDB
3J4Q EM 3500 A D/E 1-351 PDB
3J4R EM 3500 A D/E 1-351 PDB
3O7L X-ray 280 A B/D 2-351 PDB
3OW3 X-ray 190 A A 2-351 PDB
3PVB X-ray 330 A A 7-351 PDB
3QAL X-ray 170 A E 2-351 PDB
3QAM X-ray 192 A E 2-351 PDB
3TNP X-ray 230 A C/F 2-351 PDB
3TNQ X-ray 310 A B 2-351 PDB
3X2U X-ray 240 A A 1-351 PDB
3X2V X-ray 177 A A 1-351 PDB
3X2W X-ray 170 A A 1-351 PDB
4DFX X-ray 135 A E 2-351 PDB
4DFY X-ray 300 A A/E 1-351 PDB
4DFZ X-ray 200 A E 2-351 PDB
4DG0 X-ray 200 A E 2-351 PDB
4DG2 X-ray 200 A E 2-351 PDB
4DG3 X-ray 180 A E 1-351 PDB
4DH1 X-ray 200 A A 16-351 PDB
4DH3 X-ray 220 A A 2-351 PDB
4DH5 X-ray 220 A A 2-351 PDB
4DH7 X-ray 180 A A 2-351 PDB
4DH8 X-ray 230 A A 2-351 PDB
4DIN X-ray 370 A A 2-351 PDB
4HPT X-ray 215 A E 2-351 PDB
4HPU X-ray 155 A E 2-351 PDB
4IAC X-ray 215 A A 2-351 PDB
4IAD X-ray 190 A A 2-351 PDB
4IAF X-ray 220 A A 2-351 PDB
4IAI X-ray 155 A A 2-351 PDB
4IAK X-ray 160 A A 2-351 PDB
4IAY X-ray 200 A A 2-351 PDB
4IAZ X-ray 185 A A 2-351 PDB
4IB0 X-ray 187 A A 2-351 PDB
4IB1 X-ray 163 A A 2-351 PDB
4IB3 X-ray 220 A A 2-351 PDB
4NTS X-ray 290 A A/B 2-351 PDB
4NTT X-ray 350 A A/B 2-351 PDB
4O21 X-ray 195 A A 16-351 PDB
4O22 X-ray 170 A A 16-351 PDB
4WBB X-ray 280 A B 2-351 PDB
4X6Q X-ray 252 A C 2-351 PDB
4X6R X-ray 240 A A 2-351 PDB
4XW4 X-ray 182 A A 15-351 PDB
4XW5 X-ray 195 A A 15-351 PDB
4XW6 X-ray 190 A A 15-351 PDB
5JR7 X-ray 356 A A/C 2-351 PDB
5X3F X-ray 338 A B 5-351 PDB
6E21 Other 200 A A 2-351 PDB
6MM5 X-ray 195 A E 16-351 PDB
6MM6 X-ray 239 A C/E 16-351 PDB
6MM7 X-ray 185 A A/D 16-351 PDB
6MM8 X-ray 185 A C 16-351 PDB
7E0Z X-ray 216 A A 1-351 PDB
7E11 X-ray 343 A A 14-351 PDB
7E12 X-ray 280 A A 1-351 PDB
7UJX X-ray 240 A E 2-351 PDB
7V0G X-ray 163 A E 2-351 PDB
AF-P05132-F1 Predicted AlphaFoldDB

5 variants for P05132

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3388970715 33 T>N No EVA
rs3388964604 94 R>H No EVA
rs3388987507 178 Q>L No EVA
rs3399346478 242 D>N No EVA
rs3388970750 284 N>Y No EVA

No associated diseases with P05132

4 regional properties for P05132

Type Name Position InterPro Accession
domain Protein kinase domain 44 - 298 IPR000719
domain AGC-kinase, C-terminal 299 - 351 IPR000961
active_site Serine/threonine-protein kinase, active site 163 - 175 IPR008271
binding_site Protein kinase, ATP binding site 50 - 73 IPR017441

Functions

Description
EC Number 2.7.11.11 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
  • Nucleus
  • Mitochondrion
  • Membrane ; Lipid-anchor
  • Translocates into the nucleus (monomeric catalytic subunit) (By similarity)
  • The inactive holoenzyme is found in the cytoplasm
  • Distributed throughout the cytoplasm in meiotically incompetent oocytes
  • Associated to mitochondrion as meiotic competence is acquired
  • Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes
  • Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity)
  • Recruited to the cell membrane through interaction with SMO (PubMed:33886552)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

24 GO annotations of cellular component

Name Definition
acrosomal vesicle A structure in the head of a spermatozoon that contains acid hydrolases, and is concerned with the breakdown of the outer membrane of the ovum during fertilization. It lies just beneath the plasma membrane and is derived from the lysosome.
axoneme The bundle of microtubules and associated proteins that forms the core of cilia (also called flagella) in eukaryotic cells and is responsible for their movements.
cAMP-dependent protein kinase complex An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. Inactive forms of the enzyme have two regulatory chains and two catalytic chains; activation by cAMP produces two active catalytic monomers and a regulatory dimer.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
ciliary base Area of the cilium (also called flagellum) where the basal body and the axoneme are anchored to the plasma membrane. The ciliary base encompasses the distal part of the basal body, transition fibers and transition zone and is structurally and functionally very distinct from the rest of the cilium. In this area proteins are sorted and filtered before entering the cilium, and many ciliary proteins localize specifically to this area.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendritic spine A small, membranous protrusion from a dendrite that forms a postsynaptic compartment, typically receiving input from a single presynapse. They function as partially isolated biochemical and an electrical compartments. Spine morphology is variable:they can be thin, stubby, mushroom, or branched, with a continuum of intermediate morphologies. They typically terminate in a bulb shape, linked to the dendritic shaft by a restriction. Spine remodeling is though to be involved in synaptic plasticity.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
neuromuscular junction The junction between the axon of a motor neuron and a muscle fiber. In response to the arrival of action potentials, the presynaptic button releases molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane of the muscle fiber, leading to a change in post-synaptic potential.
neuron projection A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
nuclear speck A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleotide-activated protein kinase complex A protein complex that possesses nucleotide-dependent protein kinase activity. The nucleotide can be AMP (in S. pombe and human) or ADP (in S. cerevisiae).
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
plasma membrane raft A membrane raft that is part of the plasma membrane.
presynapse The part of a synapse that is part of the presynaptic cell.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
sperm flagellum A microtubule-based flagellum (or cilium) that is part of a sperm, a mature male germ cell that develops from a spermatid.
sperm midpiece The highly organized segment of the sperm flagellum which begins at the connecting piece and is characterized by the presence of 9 outer dense fibers (ODFs) that lie outside each of the 9 outer axonemal microtubule doublets and by a sheath of mitochondria that encloses the ODFs and the axoneme; the midpiece terminates about one-fourth of the way down the sperm flagellum at the annulus, which marks the beginning of the principal piece.

15 GO annotations of molecular function

Name Definition
AMP-activated protein kinase activity Catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein. This reaction requires the presence of AMP.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cAMP-dependent protein kinase activity cAMP-dependent catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein.
magnesium ion binding Binding to a magnesium (Mg) ion.
manganese ion binding Binding to a manganese ion (Mn).
protein domain specific binding Binding to a specific domain of a protein.
protein kinase A regulatory subunit binding Binding to one or both of the regulatory subunits of protein kinase A.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.
protein-containing complex binding Binding to a macromolecular complex.
small GTPase binding Binding to a small monomeric GTPase.
ubiquitin protein ligase binding Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.

28 GO annotations of biological process

Name Definition
cellular response to cold Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cold stimulus, a temperature stimulus below the optimal temperature for that organism.
cellular response to glucose stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucose stimulus.
cellular response to heat Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
cellular response to parathyroid hormone stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a parathyroid hormone stimulus.
mesoderm formation The process that gives rise to the mesoderm. This process pertains to the initial formation of the structure from unspecified parts.
modulation of chemical synaptic transmission Any process that modulates the frequency or amplitude of synaptic transmission, the process of communication from a neuron to a target (neuron, muscle, or secretory cell) across a synapse. Amplitude, in this case, refers to the change in postsynaptic membrane potential due to a single instance of synaptic transmission.
mRNA processing Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.
negative regulation of meiotic cell cycle Any process that stops, prevents or reduces the rate or extent of progression through the meiotic cell cycle.
negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Any process that stops, prevents or reduces the frequency, rate or extent of smoothened signaling pathway involved in dorsal/ventral neural tube patterning.
neural tube closure The last step in the formation of the neural tube, where the paired neural folds are brought together and fuse at the dorsal midline.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
peptidyl-threonine phosphorylation The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of cell population proliferation Any process that activates or increases the rate or extent of cell proliferation.
positive regulation of protein export from nucleus Any process that activates or increases the frequency, rate or extent of directed movement of proteins from the nucleus into the cytoplasm.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein export from nucleus The directed movement of a protein from the nucleus into the cytoplasm.
protein kinase A signaling A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase A, which occurs as a result of a single trigger reaction or compound.
protein localization to lipid droplet A process in which a protein is transported to, or maintained in, a location on or within a lipid droplet.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of bicellular tight junction assembly Any process that modulates the frequency, rate or extent of tight junction assembly.
regulation of cell cycle Any process that modulates the rate or extent of progression through the cell cycle.
regulation of cellular respiration Any process that modulates the frequency, rate or extent of cellular respiration, the enzymatic release of energy from organic compounds.
regulation of osteoblast differentiation Any process that modulates the frequency, rate or extent of osteoblast differentiation.
regulation of proteasomal protein catabolic process Any process that modulates the rate, frequency, or extent of the chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds that is mediated by the proteasome.
regulation of protein processing Any process that modulates the frequency, rate or extent of protein processing, a protein maturation process achieved by the cleavage of a peptide bond or bonds within a protein.
regulation of synaptic transmission, glutamatergic Any process that modulates the frequency, rate or extent of glutamatergic synaptic transmission, the process of communication from a neuron to another neuron across a synapse using the neurotransmitter glutamate.
sperm capacitation A process required for sperm to reach fertilization competence. Sperm undergo an incompletely understood series of morphological and molecular maturational processes, termed capacitation, involving, among other processes, protein tyrosine phosphorylation and increased intracellular calcium.
spontaneous exocytosis of neurotransmitter The release of a neurotransmitter into the synaptic cleft, where the release step is independent of the presence of calcium ions (Ca2+). The neurotransmitter is contained within a membrane-bounded vesicle, and is released by fusion of the vesicle with the presynaptic plasma membrane of a nerve cell.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P00517 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Bos taurus (Bovine) PR
Q8MJ44 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Canis lupus familiaris (Dog) (Canis familiaris) PR
Q03043 for cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B Drosophila melanogaster (Fruit fly) SS
P16911 Pka-C2 cAMP-dependent protein kinase catalytic subunit 2 Drosophila melanogaster (Fruit fly) PR
P17612 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Homo sapiens (Human) PR
P0C605 Prkg1 cGMP-dependent protein kinase 1 Mus musculus (Mouse) SS
Q922R0 Prkx cAMP-dependent protein kinase catalytic subunit PRKX Mus musculus (Mouse) PR
Q61410 Prkg2 cGMP-dependent protein kinase 2 Mus musculus (Mouse) PR
P36887 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Sus scrofa (Pig) PR
O62846 PRKACG cAMP-dependent protein kinase catalytic subunit gamma Macaca mulatta (Rhesus macaque) PR
Q7JP68 F47F2.1 cAMP-dependent protein kinase, catalytic subunit-like Caenorhabditis elegans PR
P21137 kin-1 cAMP-dependent protein kinase catalytic subunit Caenorhabditis elegans PR
10 20 30 40 50 60
MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WETPSQNTAQ LDQFDRIKTL GTGSFGRVML
70 80 90 100 110 120
VKHKESGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV
130 140 150 160 170 180
MEYVAGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY
190 200 210 220 230 240
IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF
250 260 270 280 290 300
ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT
310 320 330 340 350
TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFTE F