P05130
Gene name |
Pkc53E (PKC1, CG6622) |
Protein name |
Protein kinase C, brain isozyme |
Names |
PKC , EC 2.7.11.13 , dPKC53E, BR |
Species |
Drosophila melanogaster (Fruit fly) |
KEGG Pathway |
dme:Dmel_CG6622 |
EC number |
2.7.11.13: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
Autoinhibitory domains (AIDs)
Target domain |
339-679 (Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha) |
Relief mechanism |
Ligand binding |
Assay |
|
Target domain |
339-679 (Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha) |
Relief mechanism |
Ligand binding |
Assay |
|
Target domain |
352-594 (Protein kinase domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Target domain |
352-594 (Protein kinase domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
491-514 (Activation loop from InterPro)
Target domain |
350-608 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
491-514 (Activation loop from InterPro)
Target domain |
350-608 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
- Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell systems, 2, 89-100
- Steinberg SF (2008) "Structural basis of protein kinase C isoform function", Physiological reviews, 88, 1341-78
- Sommese RF et al. (2017) "The Role of Regulatory Domains in Maintaining Autoinhibition in the Multidomain Kinase PKCα", The Journal of biological chemistry, 292, 2873-2880
- Pears CJ et al. (1990) "Mutagenesis of the pseudosubstrate site of protein kinase C leads to activation", European journal of biochemistry, 194, 89-94
- Smith MK et al. (1990) "Specificities of autoinhibitory domain peptides for four protein kinases. Implications for intact cell studies of protein kinase function", The Journal of biological chemistry, 265, 1837-40
- Slater SJ et al. (2002) "Regulation of PKC alpha activity by C1-C2 domain interactions", The Journal of biological chemistry, 277, 15277-85
- Jones AC et al. (2020) "Hypothesis: Unifying model of domain architecture for conventional and novel protein kinase C isozymes", IUBMB life, 72, 2584-2590
- Kirwan AF et al. (2003) "Inhibition of protein kinase C catalytic activity by additional regions within the human protein kinase Calpha-regulatory domain lying outside of the pseudosubstrate sequence", The Biochemical journal, 373, 571-81
- Huang X et al. (2003) "Crystal structure of an inactive Akt2 kinase domain", Structure (London, England : 1993), 11, 21-30
- Truebestein L et al. (2021) "Structure of autoinhibited Akt1 reveals mechanism of PIP(3)-mediated activation", Proceedings of the National Academy of Sciences of the United States of America, 118,
- Lučić I et al. (2018) "Conformational sampling of membranes by Akt controls its activation and inactivation", Proceedings of the National Academy of Sciences of the United States of America, 115, E3940-E3949
- Wagner J et al. (2009) "Discovery of 3-(1H-indol-3-yl)-4-[2-(4-methylpiperazin-1-yl)quinazolin-4-yl]pyrrole-2,5-dione (AEB071), a potent and selective inhibitor of protein kinase C isotypes", Journal of medicinal chemistry, 52, 6193-6
Autoinhibited structure
Activated structure
1 structures for P05130
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P05130-F1 | Predicted | AlphaFoldDB |
1 variants for P05130
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| 437 | M>I | No |
No associated diseases with P05130
12 regional properties for P05130
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | C2 domain | 176 - 295 | IPR000008 |
| domain | Protein kinase domain | 350 - 608 | IPR000719 |
| domain | AGC-kinase, C-terminal | 609 - 679 | IPR000961 |
| domain | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | 45 - 106 | IPR002219-1 |
| domain | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | 119 - 172 | IPR002219-2 |
| active_site | Serine/threonine-protein kinase, active site | 470 - 482 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 356 - 379 | IPR017441 |
| domain | Protein kinase, C-terminal | 629 - 670 | IPR017892 |
| domain | Diacylglycerol/phorbol-ester binding | 43 - 57 | IPR020454-1 |
| domain | Diacylglycerol/phorbol-ester binding | 59 - 68 | IPR020454-2 |
| domain | Diacylglycerol/phorbol-ester binding | 81 - 92 | IPR020454-3 |
| domain | Diacylglycerol/phorbol-ester binding | 158 - 170 | IPR020454-4 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.13 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| diacylglycerol-dependent serine/threonine kinase activity | Catalysis of the reaction |
| protein serine kinase activity | Catalysis of the reactions |
| protein serine/threonine kinase activity | Catalysis of the reactions |
| zinc ion binding | Binding to a zinc ion (Zn). |
4 GO annotations of biological process
| Name | Definition |
|---|---|
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| positive regulation of clathrin-dependent endocytosis | Any process that activates or increases the frequency, rate or extent of clathrin-mediated endocytosis. |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
| regulation of hemocyte proliferation | Any process that modulates the frequency, rate or extent of hemocyte proliferation. Hemocytes are blood cells associated with a hemocoel (the cavity containing most of the major organs of the arthropod body) which are involved in defense and clotting of hemolymph, but not involved in transport of oxygen. An example of this is found in Drosophila melanogaster. |
31 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P24583 | PKC1 | Protein kinase C-like 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| P04409 | PRKCA | Protein kinase C alpha type | Bos taurus (Bovine) | EV SS |
| P05128 | PRKCG | Protein kinase C gamma type | Bos taurus (Bovine) | SS |
| P05126 | PRKCB | Protein kinase C beta type | Bos taurus (Bovine) | SS |
| Q9W0V1 | Pdk1 | 3-phosphoinositide-dependent protein kinase 1 | Drosophila melanogaster (Fruit fly) | SS |
| A1Z9X0 | aPKC | Atypical protein kinase C | Drosophila melanogaster (Fruit fly) | SS |
| A1Z7T0 | Pkn | Serine/threonine-protein kinase N | Drosophila melanogaster (Fruit fly) | SS |
| P83099 | Pkcdelta | Putative protein kinase C delta type homolog | Drosophila melanogaster (Fruit fly) | PR |
| Q8INB9 | Akt | RAC serine/threonine-protein kinase | Drosophila melanogaster (Fruit fly) | SS |
| Q9NBK5 | trc | Serine/threonine-protein kinase tricornered | Drosophila melanogaster (Fruit fly) | SS |
| P13677 | inaC | Protein kinase C, eye isozyme | Drosophila melanogaster (Fruit fly) | SS |
| P05129 | PRKCG | Protein kinase C gamma type | Homo sapiens (Human) | SS |
| P05771 | PRKCB | Protein kinase C beta type | Homo sapiens (Human) | SS |
| Q02156 | PRKCE | Protein kinase C epsilon type | Homo sapiens (Human) | SS |
| P24723 | PRKCH | Protein kinase C eta type | Homo sapiens (Human) | SS |
| P17252 | PRKCA | Protein kinase C alpha type | Homo sapiens (Human) | EV |
| P20444 | Prkca | Protein kinase C alpha type | Mus musculus (Mouse) | SS |
| P68404 | Prkcb | Protein kinase C beta type | Mus musculus (Mouse) | SS |
| P16054 | Prkce | Protein kinase C epsilon type | Mus musculus (Mouse) | PR |
| P23298 | Prkch | Protein kinase C eta type | Mus musculus (Mouse) | PR |
| P63318 | Prkcg | Protein kinase C gamma type | Mus musculus (Mouse) | SS |
| Q64617 | Prkch | Protein kinase C eta type | Rattus norvegicus (Rat) | PR |
| P63319 | Prkcg | Protein kinase C gamma type | Rattus norvegicus (Rat) | SS |
| P05696 | Prkca | Protein kinase C alpha type | Rattus norvegicus (Rat) | SS |
| P68403 | Prkcb | Protein kinase C beta type | Rattus norvegicus (Rat) | SS |
| P90980 | pkc-2 | Protein kinase C-like 2 | Caenorhabditis elegans | SS |
| Q9SUA3 | D6PKL1 | Serine/threonine-protein kinase D6PKL1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| O64682 | PID | Protein kinase PINOID | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LUL2 | WAG2 | Serine/threonine-protein kinase WAG2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| A8KBH6 | prkcb | Protein kinase C beta type | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| Q7SY24 | prkcbb | Protein kinase C beta type | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSEGSDNNGD | PQQQGAEGEA | VGENKMKSRL | RKGALKKKNV | FNVKDHCFIA | RFFKQPTFCS |
| 70 | 80 | 90 | 100 | 110 | 120 |
| HCKDFICGYQ | SGYAWMGFGK | QGFQCQVCSY | VVHKRCHEYV | TFICPGKDKG | IDSDSPKTQH |
| 130 | 140 | 150 | 160 | 170 | 180 |
| NFEPFTYAGP | TFCDHCGSLL | YGIYHQGLKC | SACDMNVHAR | CKENVPSLCG | CDHTERRGRI |
| 190 | 200 | 210 | 220 | 230 | 240 |
| YLEINVKENL | LTVQIKEGRN | LIPMDPNGLS | DPYVKVKLIP | DDKDQSKKKT | RTIKACLNPV |
| 250 | 260 | 270 | 280 | 290 | 300 |
| WNETLTYDLK | PEDKDRRILI | EVWDWDRTSR | NDFMGALSFG | ISEIIKNPTN | GWFKLLTQDE |
| 310 | 320 | 330 | 340 | 350 | 360 |
| GEYYNVPCAD | DEQDLLKLKQ | KPSQKKPMVM | RSDTNTHTSS | KKDMIRATDF | NFIKVLGKGS |
| 370 | 380 | 390 | 400 | 410 | 420 |
| FGKVLLAERK | GSEELYAIKI | LKKDVIIQDD | DVECTMIEKR | VLALGEKPPF | LVQLHSCFQT |
| 430 | 440 | 450 | 460 | 470 | 480 |
| MDRLFFVMEY | VNGGDLMFQI | QQFGKFKEPV | AVFYAAEIAA | GLFFLHTKGI | LYRDLKLDNV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| LLDADGHVKI | ADFGMCKENI | VGDKTTKTFC | GTPDYIAPEI | ILYQPYGKSV | DWWAYGVLLY |
| 550 | 560 | 570 | 580 | 590 | 600 |
| EMLVGQPPFD | GEDEEELFAA | ITDHNVSYPK | SLSKEAKEAC | KGFLTKQPNK | RLGCGSSGEE |
| 610 | 620 | 630 | 640 | 650 | 660 |
| DVRLHPFFRR | IDWEKIENRE | VQPPFKPKIK | HRKDVSNFDK | QFTSEKTDLT | PTDKVFMMNL |
| 670 | |||||
| DQSEFVGFSY | MNPEYVFSP |