P05128

SS
Gene name
PRKCG
Protein name
Protein kinase C gamma type
Names
PKC-gamma , EC 2.7.11.13
Species
Bos taurus (Bovine)
KEGG Pathway
bta:282002
EC number
2.7.11.-: Protein-serine/threonine kinases
Protein Class

Descriptions

Protein kinase C is a multi-module Ser/Thr protein kinase that transduces the abundance of signals resulting in phospholipid hydrolysis. Binding of diacylglycerol allosterically and reversibly activates these enzymes to affect their major role in maintaining cellular homeostasis. This allosteric regulation is precisely controlled by multiple mechanisms that ensure that the enzyme is only active for specific times at specific locations in response to specific signals. <br> PKC consists of a regulatory moiety (pseudosubstrate site, C1A and C1B able to bind phorbol esters and diacylglycerol, and calcium-binding C2 domain) and protein kinase domain. The kinase domain is followed by a C-Tail that is a hallmark regulatory region of AGC kinases and serves as a docking site for regulatory proteins. <br>The pseudosubstrate binds the substrate-binding cavity of the kinase domain and operates as an autoinhibitory segment. While the pseudosubstrate-C1A is the dominant autoinhibitory module and is necessary for the autoinhibition of all PKC family members, all domains in the regulatory module participate in a network of interactions to maintain autoinhibition. <br>

Autoinhibitory domains (AIDs)

20-70 (C1A domain) SS

Target domain

338-585 (Protein kinase domain)

Relief mechanism

Ligand binding

Assay

85-135 (C1B domain) SS

Target domain

338-585 (Protein kinase domain)

Relief mechanism

Ligand binding

Assay

136-162 (C1B-C2 linker) SS

Target domain

338-585 (Protein kinase domain)

Relief mechanism

Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P05128

Entry ID Method Resolution Chain Position Source
AF-P05128-F1 Predicted AlphaFoldDB

No variants for P05128

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P05128

No associated diseases with P05128

12 regional properties for P05128

Type Name Position InterPro Accession
domain C2 domain 142 - 262 IPR000008
domain Protein kinase domain 336 - 599 IPR000719
domain AGC-kinase, C-terminal 600 - 670 IPR000961
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 20 - 71 IPR002219-1
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 85 - 137 IPR002219-2
active_site Serine/threonine-protein kinase, active site 461 - 473 IPR008271
binding_site Protein kinase, ATP binding site 342 - 365 IPR017441
domain Protein kinase, C-terminal 627 - 660 IPR017892
domain Diacylglycerol/phorbol-ester binding 18 - 32 IPR020454-1
domain Diacylglycerol/phorbol-ester binding 34 - 43 IPR020454-2
domain Diacylglycerol/phorbol-ester binding 47 - 58 IPR020454-3
domain Diacylglycerol/phorbol-ester binding 124 - 136 IPR020454-4

Functions

Description
EC Number 2.7.11.- Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cytoplasm, perinuclear region
  • Cell membrane ; Peripheral membrane protein
  • Synapse, synaptosome
  • Cell projection, dendrite
  • Translocates to synaptic membranes on stimulation
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
synapse The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
zinc ion binding Binding to a zinc ion (Zn).

10 GO annotations of biological process

Name Definition
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
negative regulation of neuron apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process in neurons.
negative regulation of proteasomal protein catabolic process Any process that stops, prevents or reduces the frequency, rate or extent of proteasomal protein catabolic process.
negative regulation of protein ubiquitination Any process that stops, prevents, or reduces the frequency, rate or extent of the addition of ubiquitin groups to a protein.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
regulation of circadian rhythm Any process that modulates the frequency, rate or extent of a circadian rhythm. A circadian rhythm is a biological process in an organism that recurs with a regularity of approximately 24 hours.
regulation of response to food Any process that modulates the frequency, rate or extent of a response to a food stimulus.
response to morphine Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a morphine stimulus. Morphine is an opioid alkaloid, isolated from opium, with a complex ring structure.
response to pain Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a pain stimulus. Pain stimuli cause activation of nociceptors, peripheral receptors for pain, include receptors which are sensitive to painful mechanical stimuli, extreme heat or cold, and chemical stimuli.
rhythmic process Any process pertinent to the generation and maintenance of rhythms in the physiology of an organism.

19 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
P05126 PRKCB Protein kinase C beta type Bos taurus (Bovine) SS
A1A4I4 PKN1 Serine/threonine-protein kinase N1 Bos taurus (Bovine) SS
P04409 PRKCA Protein kinase C alpha type Bos taurus (Bovine) EV SS
A2VDV2 STK38 Serine/threonine-protein kinase 38 Bos taurus (Bovine) SS
P13677 inaC Protein kinase C, eye isozyme Drosophila melanogaster (Fruit fly) SS
P05130 Pkc53E Protein kinase C, brain isozyme Drosophila melanogaster (Fruit fly) SS
P05771 PRKCB Protein kinase C beta type Homo sapiens (Human) SS
P17252 PRKCA Protein kinase C alpha type Homo sapiens (Human) EV
P05129 PRKCG Protein kinase C gamma type Homo sapiens (Human) SS
P20444 Prkca Protein kinase C alpha type Mus musculus (Mouse) SS
P68404 Prkcb Protein kinase C beta type Mus musculus (Mouse) SS
P63318 Prkcg Protein kinase C gamma type Mus musculus (Mouse) SS
P05696 Prkca Protein kinase C alpha type Rattus norvegicus (Rat) SS
P68403 Prkcb Protein kinase C beta type Rattus norvegicus (Rat) SS
P63319 Prkcg Protein kinase C gamma type Rattus norvegicus (Rat) SS
P90980 pkc-2 Protein kinase C-like 2 Caenorhabditis elegans SS
A8KBH6 prkcb Protein kinase C beta type Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
Q7SY24 prkcbb Protein kinase C beta type Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
RPLFCRKGAL RQKVVHEVKS HKFTARFFKQ PTFCSHCTDF IWGIGKQGLQ CQVCSFVVHR
70 80 90 100 110 120
RCHEFVTFEC PGAGKGPQTD DPRNKHKFRL HSYSSPTFCD HCGSLLYGLV HQGMKCSCCE
130 140 150 160 170 180
MNVHRRCVRS VPSLCGVDHT ERRGRLQLEI RAPTSDEIHV TVGEARNLIP MDPNGLSDPY
190 200 210 220 230 240
VKLKLIPDPR NLTKQKTRTV KATLNPVWNE TFVFNLKPGD VERRLSVEVW DWDRTSRNDF
250 260 270 280 290 300
MGAMSFGVSE LLKAPVDGWY KLLNQEEGEY YNVPVADADN CNLLQKFEAC NYPLELYERV
310 320 330 340 350 360
RTGPSSSPIP SPSPSPTDSK RCFFGASPGR LHISDFSFLM VLGKGSFGKV MLAERRGSDE
370 380 390 400 410 420
LYAIKILKKD VIVQDDDVDC TLVEKRVLAL GGRGPGGRPH FLTQLHSTFQ TPDRLYFVME
430 440 450 460 470 480
YVTGGDLMYH IQQLGKFKEP HAAFYAAEIA IGLFFLHNQG IIYRDLKLDN VMLDAEGHIK
490 500 510 520 530 540
ITDFGMCKEN VFPGSTTRTF CGTPDYIAPE IIAYQPYGKS VDWWSFGVLL YEMLAGQPPF
550 560 570 580 590 600
DGEDEEELFQ AIMEQTVTYP KSLSREAVAI CKGFLTKHPA KRLGSGPDGE PTIRAHGFFR
610 620 630 640 650 660
WIDWDRLERL EIAPPFRPRP CGRSGENFDK FFTRAAPALT PPDRLVLASI DQAEFQGFTY
670 680
VNPDFVHPDA RSPISPTPVP VM