P00517

PR
Gene name
PRKACA
Protein name
cAMP-dependent protein kinase catalytic subunit alpha
Names
PKA C-alpha
Species
Bos taurus (Bovine)
KEGG Pathway
bta:282322
EC number
2.7.11.11: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-60 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

184-204 (Activation loop from InterPro)

Target domain

44-298 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

91 structures for P00517

Entry ID Method Resolution Chain Position Source
1Q24 X-ray 260 A A 2-351 PDB
1Q61 X-ray 210 A A 2-351 PDB
1Q62 X-ray 230 A A 2-351 PDB
1Q8T X-ray 200 A A 2-351 PDB
1Q8U X-ray 190 A A 2-351 PDB
1Q8W X-ray 220 A A 2-351 PDB
1SMH X-ray 204 A A 2-351 PDB
1STC X-ray 230 A E 2-351 PDB
1SVE X-ray 249 A A 2-351 PDB
1SVG X-ray 202 A A 2-351 PDB
1SVH X-ray 230 A A 2-351 PDB
1SZM X-ray 250 A A/B 2-351 PDB
1VEB X-ray 289 A A 2-351 PDB
1XH4 X-ray 245 A A 2-351 PDB
1XH5 X-ray 205 A A 2-351 PDB
1XH6 X-ray 190 A A 2-351 PDB
1XH7 X-ray 247 A A 2-351 PDB
1XH8 X-ray 160 A A 2-351 PDB
1XH9 X-ray 164 A A 2-351 PDB
1XHA X-ray 246 A A 2-351 PDB
1YDR X-ray 220 A E 2-351 PDB
1YDS X-ray 220 A E 2-351 PDB
1YDT X-ray 230 A E 2-351 PDB
2C1A X-ray 195 A A 2-351 PDB
2C1B X-ray 200 A A 2-351 PDB
2F7E X-ray 200 A E 1-351 PDB
2F7X X-ray 190 A E 1-351 PDB
2F7Z X-ray 300 A E 1-351 PDB
2GFC X-ray 187 A A 2-351 PDB
2GNF X-ray 228 A A 2-351 PDB
2GNG X-ray 187 A A 2-351 PDB
2GNH X-ray 205 A A 2-351 PDB
2GNI X-ray 227 A A 2-351 PDB
2GNJ X-ray 228 A A 2-351 PDB
2GNL X-ray 260 A A 2-351 PDB
2JDS X-ray 200 A A 2-351 PDB
2JDT X-ray 215 A A 2-351 PDB
2JDV X-ray 208 A A 2-351 PDB
2OH0 X-ray 220 A E 1-351 PDB
2OJF X-ray 210 A E 1-351 PDB
2UVX X-ray 200 A A 2-351 PDB
2UVY X-ray 195 A A 2-351 PDB
2UVZ X-ray 194 A A 2-351 PDB
2UW0 X-ray 200 A A 2-351 PDB
2UW3 X-ray 219 A A 2-351 PDB
2UW4 X-ray 200 A A 2-351 PDB
2UW5 X-ray 214 A A 2-351 PDB
2UW6 X-ray 223 A A 2-351 PDB
2UW7 X-ray 210 A A 2-351 PDB
2UW8 X-ray 200 A A 2-351 PDB
2UZT X-ray 210 A A 16-351 PDB
2UZU X-ray 240 A E 16-351 PDB
2UZV X-ray 250 A A 16-351 PDB
2UZW X-ray 220 A E 16-351 PDB
2VNW X-ray 209 A A 1-351 PDB
2VNY X-ray 196 A A 1-351 PDB
2VO0 X-ray 194 A A 1-351 PDB
2VO3 X-ray 198 A A 1-351 PDB
2VO6 X-ray 197 A A 1-351 PDB
2VO7 X-ray 198 A A 1-351 PDB
3AG9 X-ray 200 A A/B 1-351 PDB
3BWJ X-ray 230 A A 2-351 PDB
3DND X-ray 226 A A 2-351 PDB
3DNE X-ray 200 A A 2-351 PDB
3E8C X-ray 220 A A/B/C/D/E/F 2-351 PDB
3E8E X-ray 200 A A/B/E/I/L/P 2-351 PDB
3KKV X-ray 180 A A 2-351 PDB
3ZO1 X-ray 200 A A 1-351 PDB
3ZO2 X-ray 198 A A 1-351 PDB
3ZO3 X-ray 210 A A 1-351 PDB
3ZO4 X-ray 165 A A 1-351 PDB
4AXA X-ray 190 A A 1-351 PDB
4C33 X-ray 170 A A 1-351 PDB
4C34 X-ray 178 A A 1-351 PDB
4C35 X-ray 219 A A 1-351 PDB
4C36 X-ray 198 A A 1-351 PDB
4C37 X-ray 170 A A 1-351 PDB
4C38 X-ray 158 A A 1-351 PDB
4IE9 X-ray 192 A A 1-351 PDB
4IJ9 X-ray 255 A A 2-351 PDB
4YXR X-ray 200 A A 2-351 PDB
4YXS X-ray 211 A A 2-351 PDB
4Z83 X-ray 180 A E 2-351 PDB
4Z84 X-ray 155 A A 1-351 PDB
5VHB X-ray 161 A A 1-351 PDB
5VI9 X-ray 195 A A 1-351 PDB
5VIB X-ray 237 A A 1-351 PDB
6E99 X-ray 188 A A 1-351 PDB
6E9L X-ray 280 A A 1-351 PDB
8SF8 X-ray 170 A A 1-351 PDB
AF-P00517-F1 Predicted AlphaFoldDB

65 variants for P00517

Variant ID(s) Position Change Description Diseaes Association Provenance
rs437880744 16 V>A No EVA
rs474365808 19 F>C No EVA
rs456161929 19 F>I No EVA
rs453651433 24 K>N No EVA
rs472079162 30 K>T No EVA
rs440619596 32 E>D No EVA
rs459072430 34 P>R No EVA
rs477787135 36 Q>R No EVA
rs476556558 39 A>P No EVA
rs443640113 40 H>P No EVA
rs443640113 40 H>R No EVA
rs480185575 43 Q>H No EVA
rs461785768 43 Q>K No EVA
rs447518825 45 E>G No EVA
rs478132306 49 T>P No EVA
rs444833757 50 L>R No EVA
rs469797547 54 S>P No EVA
rs481920938 55 F>C No EVA
rs448961914 55 F>L No EVA
rs468789039 57 R>G No EVA
rs435752071 58 V>G No EVA
rs454290690 60 L>R No EVA
rs135880103 64 M>R No EVA
rs433461975 65 E>D No EVA
rs133097611 66 T>P No EVA
rs443708200 70 Y>* No EVA
rs455645476 74 I>S No EVA
rs441026838 75 L>F No EVA
rs459540400 75 L>P No EVA
rs438640633 78 Q>K No EVA
rs876116479 80 V>G No EVA
rs876556665 89 T>P No EVA
rs876248256 97 Q>H No EVA
rs876652318 97 Q>R No EVA
rs876017790 98 A>E No EVA
rs876017790 98 A>G No EVA
rs477996790 132 H>R No EVA
rs463386636 142 P>R No EVA
rs442366602 169 K>T No EVA
rs463906637 183 V>G No EVA
rs482537925 191 R>C No EVA
rs449270596 196 T>S No EVA
rs521811997 199 L>S No EVA
rs479991410 202 T>N No EVA
rs465189161 207 A>D No EVA
rs446832560 223 W>C No EVA
rs465477331 225 L>Q No EVA
rs477454735 227 V>G No EVA
rs450767996 232 M>R No EVA
rs469144504 241 A>S No EVA
rs436328147 242 D>A No EVA
rs448263515 243 Q>P No EVA
rs480269950 261 H>R No EVA
rs447164635 263 S>R No EVA
rs459451891 269 L>M No EVA
rs477851364 276 V>G No EVA
rs462178229 280 K>N No EVA
rs464749729 302 D>A No EVA
rs438316858 307 Y>* No EVA
rs468819393 309 R>G No EVA
rs435590673 310 K>E No EVA
rs453937304 310 K>N No EVA
rs453248079 311 V>G No EVA
rs471656339 314 P>A No EVA
rs438423959 323 G>V No EVA

No associated diseases with P00517

4 regional properties for P00517

Type Name Position InterPro Accession
domain Protein kinase domain 44 - 298 IPR000719
domain AGC-kinase, C-terminal 299 - 351 IPR000961
active_site Serine/threonine-protein kinase, active site 163 - 175 IPR008271
binding_site Protein kinase, ATP binding site 50 - 73 IPR017441

Functions

Description
EC Number 2.7.11.11 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
  • Membrane ; Lipid-anchor
  • Nucleus
  • Mitochondrion
  • Translocates into the nucleus (monomeric catalytic subunit)
  • The inactive holoenzyme is found in the cytoplasm
  • Distributed throughout the cytoplasm in meiotically incompetent oocytes
  • Associated to mitochondrion as meiotic competence is acquired
  • Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity)
  • Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity)
  • Recruited to the cell membrane through interaction with SMO (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

14 GO annotations of cellular component

Name Definition
acrosomal vesicle A structure in the head of a spermatozoon that contains acid hydrolases, and is concerned with the breakdown of the outer membrane of the ovum during fertilization. It lies just beneath the plasma membrane and is derived from the lysosome.
axoneme The bundle of microtubules and associated proteins that forms the core of cilia (also called flagella) in eukaryotic cells and is responsible for their movements.
cAMP-dependent protein kinase complex An enzyme complex, composed of regulatory and catalytic subunits, that catalyzes protein phosphorylation. Inactive forms of the enzyme have two regulatory chains and two catalytic chains; activation by cAMP produces two active catalytic monomers and a regulatory dimer.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
ciliary base Area of the cilium (also called flagellum) where the basal body and the axoneme are anchored to the plasma membrane. The ciliary base encompasses the distal part of the basal body, transition fibers and transition zone and is structurally and functionally very distinct from the rest of the cilium. In this area proteins are sorted and filtered before entering the cilium, and many ciliary proteins localize specifically to this area.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
dendritic spine A small, membranous protrusion from a dendrite that forms a postsynaptic compartment, typically receiving input from a single presynapse. They function as partially isolated biochemical and an electrical compartments. Spine morphology is variable:they can be thin, stubby, mushroom, or branched, with a continuum of intermediate morphologies. They typically terminate in a bulb shape, linked to the dendritic shaft by a restriction. Spine remodeling is though to be involved in synaptic plasticity.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
neuromuscular junction The junction between the axon of a motor neuron and a muscle fiber. In response to the arrival of action potentials, the presynaptic button releases molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane of the muscle fiber, leading to a change in post-synaptic potential.
nuclear speck A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane raft A membrane raft that is part of the plasma membrane.
sperm flagellum A microtubule-based flagellum (or cilium) that is part of a sperm, a mature male germ cell that develops from a spermatid.

13 GO annotations of molecular function

Name Definition
AMP-activated protein kinase activity Catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein. This reaction requires the presence of AMP.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cAMP-dependent protein kinase activity cAMP-dependent catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein.
magnesium ion binding Binding to a magnesium (Mg) ion.
manganese ion binding Binding to a manganese ion (Mn).
protein domain specific binding Binding to a specific domain of a protein.
protein kinase A regulatory subunit binding Binding to one or both of the regulatory subunits of protein kinase A.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.
ubiquitin protein ligase binding Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.

23 GO annotations of biological process

Name Definition
cellular response to cold Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cold stimulus, a temperature stimulus below the optimal temperature for that organism.
cellular response to glucose stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucose stimulus.
cellular response to heat Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
cellular response to parathyroid hormone stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a parathyroid hormone stimulus.
mesoderm formation The process that gives rise to the mesoderm. This process pertains to the initial formation of the structure from unspecified parts.
modulation of chemical synaptic transmission Any process that modulates the frequency or amplitude of synaptic transmission, the process of communication from a neuron to a target (neuron, muscle, or secretory cell) across a synapse. Amplitude, in this case, refers to the change in postsynaptic membrane potential due to a single instance of synaptic transmission.
mRNA processing Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.
negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Any process that stops, prevents or reduces the frequency, rate or extent of smoothened signaling pathway involved in dorsal/ventral neural tube patterning.
neural tube closure The last step in the formation of the neural tube, where the paired neural folds are brought together and fuse at the dorsal midline.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
peptidyl-threonine phosphorylation The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of protein export from nucleus Any process that activates or increases the frequency, rate or extent of directed movement of proteins from the nucleus into the cytoplasm.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein export from nucleus The directed movement of a protein from the nucleus into the cytoplasm.
protein kinase A signaling A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase A, which occurs as a result of a single trigger reaction or compound.
protein localization to lipid droplet A process in which a protein is transported to, or maintained in, a location on or within a lipid droplet.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of bicellular tight junction assembly Any process that modulates the frequency, rate or extent of tight junction assembly.
regulation of cell cycle Any process that modulates the rate or extent of progression through the cell cycle.
regulation of osteoblast differentiation Any process that modulates the frequency, rate or extent of osteoblast differentiation.
regulation of proteasomal protein catabolic process Any process that modulates the rate, frequency, or extent of the chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds that is mediated by the proteasome.
regulation of protein processing Any process that modulates the frequency, rate or extent of protein processing, a protein maturation process achieved by the cleavage of a peptide bond or bonds within a protein.
sperm capacitation A process required for sperm to reach fertilization competence. Sperm undergo an incompletely understood series of morphological and molecular maturational processes, termed capacitation, involving, among other processes, protein tyrosine phosphorylation and increased intracellular calcium.

11 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P00516 PRKG1 cGMP-dependent protein kinase 1 Bos taurus (Bovine) SS
Q8MJ44 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Canis lupus familiaris (Dog) (Canis familiaris) PR
Q03043 for cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B Drosophila melanogaster (Fruit fly) SS
P16911 Pka-C2 cAMP-dependent protein kinase catalytic subunit 2 Drosophila melanogaster (Fruit fly) PR
P17612 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Homo sapiens (Human) PR
Q922R0 Prkx cAMP-dependent protein kinase catalytic subunit PRKX Mus musculus (Mouse) PR
P05132 Prkaca cAMP-dependent protein kinase catalytic subunit alpha Mus musculus (Mouse) PR
P36887 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Sus scrofa (Pig) PR
O62846 PRKACG cAMP-dependent protein kinase catalytic subunit gamma Macaca mulatta (Rhesus macaque) PR
Q7JP68 F47F2.1 cAMP-dependent protein kinase, catalytic subunit-like Caenorhabditis elegans PR
P21137 kin-1 cAMP-dependent protein kinase catalytic subunit Caenorhabditis elegans PR
10 20 30 40 50 60
MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WENPAQNTAH LDQFERIKTL GTGSFGRVML
70 80 90 100 110 120
VKHMETGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV
130 140 150 160 170 180
MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY
190 200 210 220 230 240
IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF
250 260 270 280 290 300
ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT
310 320 330 340 350
TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE F