O97902
SS
Gene name |
ASAP1 (DDEF1) |
Protein name |
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 |
Names |
130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein , ADP-ribosylation factor-directed GTPase-activating protein 1 , ARF GTPase-activating protein 1 , Development and differentiation-enhancing factor 1 , DEF-1 , Differentiation-enhancing factor 1 , PIP2-dependent ARF1 GAP |
Species |
Bos taurus (Bovine) |
KEGG Pathway |
bta:327705 |
EC number |
|
Protein Class |
|
Descriptions
ASAP1 is an Arf GTPase-activating protein (GAP) that functions on membrane surfaces to catalyze the hydrolysis of GTP bound to Arf. ASAP1 has a BAR domain which functions as membrane curvature sensors or as inducers of membrane curvature. The BAR domain influences GAP activity by binding to the PH domain and/or Arf GAP domain. The deletion of the entire BAR domain or the N-terminal extension increased GAP activity.
Autoinhibitory domains (AIDs)
Target domain |
329-416 (PH domain);442-565 (Arf GAP domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for O97902
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O97902-F1 | Predicted | AlphaFoldDB |
No variants for O97902
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O97902 | |||||
No associated diseases with O97902
8 regional properties for O97902
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Arf GTPase activating protein | 442 - 565 | IPR001164 |
| domain | SH3 domain | 1067 - 1129 | IPR001452 |
| domain | Pleckstrin homology domain | 327 - 421 | IPR001849 |
| repeat | Ankyrin repeat | 589 - 671 | IPR002110 |
| domain | BAR domain | 54 - 286 | IPR004148 |
| domain | ASAP, PH domain | 319 - 425 | IPR037844 |
| domain | ASAP1, BAR domain | 54 - 268 | IPR037928 |
| domain | ASAP1, SH3 domain | 1071 - 1127 | IPR038016 |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| Golgi membrane | The lipid bilayer surrounding any of the compartments of the Golgi apparatus. |
| podosome | An actin-rich adhesion structure characterized by formation upon cell substrate contact and localization at the substrate-attached part of the cell, contain an F-actin-rich core surrounded by a ring structure containing proteins such as vinculin and talin, and have a diameter of 0.5 mm. |
| trans-Golgi network membrane | The lipid bilayer surrounding any of the compartments that make up the trans-Golgi network. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| GTPase activator activity | Binds to and increases the activity of a GTPase, an enzyme that catalyzes the hydrolysis of GTP. |
| metal ion binding | Binding to a metal ion. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| cilium assembly | The assembly of a cilium, a specialized eukaryotic organelle that consists of a filiform extrusion of the cell surface. Each cilium is bounded by an extrusion of the cytoplasmic membrane, and contains a regular longitudinal array of microtubules, anchored basally in a centriole. |
| positive regulation of membrane tubulation | Any process that activates or increases the frequency, rate or extent of membrane tubulation. |
| protein localization to cilium | A process in which a protein is transported to, or maintained in, a location within a cilium. |
15 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A5PK26 | ACAP1 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 | Bos taurus (Bovine) | SS |
| A1Z7A6 | Asap | ArfGAP with SH3 domain, ANK repeat and PH domain-containing protein | Drosophila melanogaster (Fruit fly) | SS |
| Q15027 | ACAP1 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 | Homo sapiens (Human) | EV |
| O43150 | ASAP2 | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 | Homo sapiens (Human) | SS |
| Q15057 | ACAP2 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 | Homo sapiens (Human) | PR |
| Q8TDY4 | ASAP3 | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 | Homo sapiens (Human) | SS |
| Q9ULH1 | ASAP1 | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 | Homo sapiens (Human) | EV |
| Q8K2H4 | Acap1 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 | Mus musculus (Mouse) | SS |
| Q5U464 | Asap3 | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 | Mus musculus (Mouse) | SS |
| Q6ZQK5 | Acap2 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 | Mus musculus (Mouse) | PR |
| Q7SIG6 | Asap2 | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 | Mus musculus (Mouse) | SS |
| Q9QWY8 | Asap1 | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 | Mus musculus (Mouse) | SS |
| Q1AAU6 | Asap1 | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 | Rattus norvegicus (Rat) | SS |
| Q9C6C3 | AGD2 | ADP-ribosylation factor GTPase-activating protein AGD2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SMX5 | AGD4 | ADP-ribosylation factor GTPase-activating protein AGD4 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MRSSASRLSS | FSSRDSLWNR | MPDQISVSEF | IAETTEDYNS | PTTSSFTTRL | HNCRNTVTLL |
| 70 | 80 | 90 | 100 | 110 | 120 |
| EEALDQDRTA | LQKVKKSVKA | IYNSGQDHVQ | NEENYAQVLD | KFGSNFLSRD | NPDLGTAFVK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| FSTLTKELST | LLKNLLQGLS | HNVIFTLDSL | LKGDLKGVKG | DLKKPFDKAW | KDYETKFTKI |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EKEKREHAKQ | HGMIRTEITG | AEIAEEMEKE | RRLFQLQMCE | YLIKVNEIKT | KKGVDLLQNL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| IKYYHAQCNF | FQDGLKTADK | LKQYIEKLAA | DLYNIKQTQD | EEKKQLTALR | DLIKSSLQLD |
| 310 | 320 | 330 | 340 | 350 | 360 |
| QKESRRDSQS | RQGGYSMHQL | QGNKEYGSEK | KGYLLKKSDG | IRKVWQRRKC | SVKNGILTIS |
| 370 | 380 | 390 | 400 | 410 | 420 |
| HATSNRQPAK | LNLLTCQVKP | NAEDKKSFDL | ISHNRTYHFQ | AEDEQDYVAW | ISVLTNSKEE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| ALTMAFRGEQ | SAGESSLEEL | TKAIIEDVQR | LPGNDVCCDC | GSAEPTWLST | NLGILTCIEC |
| 490 | 500 | 510 | 520 | 530 | 540 |
| SGIHREMGVH | ISRIQSLELD | KLGTSELLLA | KNVGNNSFND | IMEANLPSPS | PKPTPSSDMT |
| 550 | 560 | 570 | 580 | 590 | 600 |
| VRKEYITAKY | VDHRFSRKTC | SSSSAKLNEL | LEAIKSRDLL | ALIQVYAEGV | ELMEPLLEPG |
| 610 | 620 | 630 | 640 | 650 | 660 |
| QELGETALHL | AVRTADQTSL | HLVDFLVQNC | GNLDKQTALG | NTALHYCSMY | SKPECLKLLL |
| 670 | 680 | 690 | 700 | 710 | 720 |
| RSKPTVDVVN | QAGETALDIA | KRLKATQCED | LLSQAKSGKF | NPHVHVEYEW | NLRQEEMDES |
| 730 | 740 | 750 | 760 | 770 | 780 |
| DDDLDDKPSP | IKKERSPRPQ | SFCHSSSISP | QDKLSLPGFS | TPRDKQRLSY | GAFTNQIFVS |
| 790 | 800 | 810 | 820 | 830 | 840 |
| TSTDSPTSPI | AEAPPLPPRN | ATKGPPGPPS | TLPLSTQTSS | GSSTLSKKRS | PPPPPGHKRT |
| 850 | 860 | 870 | 880 | 890 | 900 |
| LSDPPSPLPH | GPPNKGAVPW | GNDVGPSSSS | KTTNKFEGLS | QQSSTGSAKT | ALGPRVLPKL |
| 910 | 920 | 930 | 940 | 950 | 960 |
| PQKVALRKTE | TSHHLSLDKA | NVPPEIFQKS | SQLTELPQKP | PPGDLPPKPT | ELAPKPPIGD |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| LPPKPGELPP | KPQLGDLPPK | PQLADLPPKP | QVKDLPPKPQ | LGELLAKPQT | GDASPKAQPP |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| LELTPKSHPA | DLSPNVPKQA | SEDTNDLTPT | LPETPVPLPR | KINTGKSKVR | RVKTIYDCQA |
| 1090 | 1100 | 1110 | 1120 | ||
| DNDDELTFME | GEVIVVTGEE | DQEWWIGHIE | GQPERKGVFP | VSFVHILSD |