O97143

EV
Gene name
SAK (CG7186)
Protein name
Serine/threonine-protein kinase PLK4
Names
Polo-like kinase 4, PLK-4, Serine/threonine-protein kinase SAK
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG7186
EC number
2.7.11.21: Protein-serine/threonine kinases
Protein Class
SERINE/THREONINE-PROTEIN KINASE PLK (PTHR24345)

Descriptions

Polo-like kinase 4 (or serine/threonine-protein kinase, Plk4) is a master regulator of centriole duplication. The linker region between kinase domain and POLO box1 (PB1) domain is involved in autoinhibition by blocking the active site of the kinase domain. Binding of non-phosphorylated Asl-A to Plk4 and phosphorylation of residues within the linker region relieve autoinhibition. Phosphorylation of the activation loop within the kinase domain is also important for kinase activity.

Autoinhibitory domains (AIDs)

318-380 (Linker 1 region) EV

Target domain

14-267 (Protein kinase domain)

Relief mechanism

Partner binding, PTM

Assay

Deletion assay, Mutagenesis experiment

Accessory elements

155-178 (Activation loop from InterPro)

Target domain

14-267 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

5 structures for O97143

Entry ID Method Resolution Chain Position Source
4G7N X-ray 230 A A/B 382-602 PDB
4NK7 X-ray 323 A A 383-601 PDB
5LHX X-ray 153 A A/B 657-745 PDB
7RL3 X-ray 175 A A/B 657-745 PDB
AF-O97143-F1 Predicted AlphaFoldDB

No variants for O97143

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O97143

No associated diseases with O97143

7 regional properties for O97143

Type Name Position InterPro Accession
domain Protein kinase domain 14 - 267 IPR000719
domain POLO box domain 670 - 713 IPR000959
active_site Tyrosine-protein kinase, active site 134 - 146 IPR008266
binding_site Protein kinase, ATP binding site 20 - 43 IPR017441
domain Plk4, C-terminal polo-box domain 657 - 738 IPR033696
domain Plk4, second cryptic polo-box domain 498 - 602 IPR033698
domain Plk4, first cryptic polo-box domain 381 - 498 IPR033699

Functions

Description
EC Number 2.7.11.21 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
  • Colocalizes with Sas-4 and Patronin at the microtubule organizing center (PubMed:20946984)
  • Colocalizes with Alms1a at the mother centrosome in male germ stem cells (PubMed:32965218)
PANTHER Family PTHR24345 SERINE/THREONINE-PROTEIN KINASE PLK
PANTHER Subfamily PTHR24345:SF94 SERINE_THREONINE-PROTEIN KINASE PLK4
PANTHER Protein Class non-receptor serine/threonine protein kinase
protein modifying enzyme
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
centriole A cellular organelle, found close to the nucleus in many eukaryotic cells, consisting of a small cylinder with microtubular walls, 300-500 nm long and 150-250 nm in diameter. It contains nine short, parallel, peripheral microtubular fibrils, each fibril consisting of one complete microtubule fused to two incomplete microtubules. Cells usually have two centrioles, lying at right angles to each other. At division, each pair of centrioles generates another pair and the twin pairs form the pole of the mitotic spindle.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
identical protein binding Binding to an identical protein or proteins.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

11 GO annotations of biological process

Name Definition
centriole replication The cell cycle process in which a daughter centriole is formed perpendicular to an existing centriole. An immature centriole contains a ninefold radially symmetric array of single microtubules; mature centrioles consist of a radial array of nine microtubule triplets, doublets, or singlets depending upon the species and cell type. Duplicated centrioles also become the ciliary basal body in cells that form cilia during G0.
centrosome cycle The cell cycle process in which centrosome duplication and separation takes place. The centrosome cycle can operate with a considerable degree of independence from other processes of the cell cycle.
male meiotic nuclear division A cell cycle process by which the cell nucleus divides as part of a meiotic cell cycle in the male germline.
mitotic cell cycle Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent.
positive regulation of protein catabolic process Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways resulting in the breakdown of a protein by the destruction of the native, active configuration, with or without the hydrolysis of peptide bonds.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
regulation of centriole replication Any process that modulates the frequency, rate or extent of the formation of a daughter centriole of an existing centriole.
regulation of cytokinesis Any process that modulates the frequency, rate or extent of the division of the cytoplasm of a cell and its separation into two daughter cells.
regulation of protein stability Any process that affects the structure and integrity of a protein, altering the likelihood of its degradation or aggregation.
sperm axoneme assembly The assembly and organization of the sperm flagellar axoneme, the bundle of microtubules and associated proteins that forms the core of the eukaryotic sperm flagellum, and is responsible for movement.
syncytial blastoderm mitotic cell cycle Mitotic division cycles 10 to 13 of the insect embryo. This is the second phase of the syncytial period where nuclei divide in a common cytoplasm without cytokinesis. The majority of migrating nuclei reach the embryo surface during cycle 10, after which they divide less synchronously than before, and the syncytial blastoderm cycles lengthen progressively.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2TA25 PLK1 Serine/threonine-protein kinase PLK1 Bos taurus (Bovine) SS
P52304 polo Serine/threonine-protein kinase polo Drosophila melanogaster (Fruit fly) SS
P53350 PLK1 Serine/threonine-protein kinase PLK1 Homo sapiens (Human) EV
Q07832 Plk1 Serine/threonine-protein kinase PLK1 Mus musculus (Mouse) EV
Q62673 Plk1 Serine/threonine-protein kinase PLK1 Rattus norvegicus (Rat) SS
P34331 plk-1 Serine/threonine-protein kinase plk-1 Caenorhabditis elegans SS
Q20845 plk-3 Serine/threonine-protein kinase plk-3 Caenorhabditis elegans PR
Q9N2L7 plk-2 Serine/threonine-protein kinase plk-2 Caenorhabditis elegans SS
P62205 plk1 Serine/threonine-protein kinase PLK1 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
10 20 30 40 50 60
MLSNRAFGET IEDYEVQHLL GKGGFATVYK ARCLHTHQDV AIKMIDKKLI QGTGLTNRVR
70 80 90 100 110 120
QEVEIHSRLK HPSVLQLYTF FQDANYVYLV LELAHNGELH RYMNHIARPF TETEAASILK
130 140 150 160 170 180
QVVAGLLYLH SHNIMHRDIS LSNLLLSREM HVKIADFGLA TQLKRPDERH MTMCGTPNYI
190 200 210 220 230 240
SPEVVSRTSH GLPADVWSVG CMLYTLLVGR PPFETDAVQS TLNKVVMSEY IMPAHLSYEA
250 260 270 280 290 300
QDLINKLLKK LPHERITLEA VLCHPFMLKC SNGGHSAPGA LNVFSQSMES GDSGIITFAS
310 320 330 340 350 360
SDSRNSQQIR SVENSGPQQV LPQIREEFKQ VHHKLPYEQT GLFGQASTGL AEPNWPGAAK
370 380 390 400 410 420
SSAFCMEAGN VPNSKQASLK EDRISVPPLN TKRLLPTRYK TKNAIMSILR NGEVVLEFLK
430 440 450 460 470 480
FRPTYNEDRI NDICRISDDG QRIIIYQPDP GRGLPVREQP PDLQIPSGDC VYNYDNLPSK
490 500 510 520 530 540
HWKKYIYGAR FVGLVKSKTP KVTYFSTLGK CQLMETMTDF EIRFYSGAKL LKTPSEGLKV
550 560 570 580 590 600
YDRNGMLLSD YSCSESRSLI EHGNECFTHC VNISNALEVA QTKDNSCFPV TIGRRPITDV
610 620 630 640 650 660
QPAQRLDGLR DTTNIAFSTP KSNQGSINFS LSTISSTRNT SDFGTNCSRS NMLAAHQNIP
670 680 690 700 710 720
IKRINVPEIG IATELSHGVV QVQFYDGSVV SVIPSMQGGG ITYTQPNGTS THFGKGDDLP
730 740 750 760
FPVRDRVGQI PNIQLKLKTA PLLGSGRKTD YNNAMTPKTT TPYYNRMLL