O81108

EV
Gene name
ACA2
Protein name
Calcium-transporting ATPase 2, plasma membrane-type
Names
Ca(2+)-ATPase isoform 2
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT4G37640
EC number
7.2.2.10: Linked to the hydrolysis of a nucleoside triphosphate
Protein Class
CATION TRANSPORTING ATPASE (PTHR24093)

Descriptions

ACA2 is an Arabidopsis Ca2+-ATPase isoform 2. ACA2 catalyzes the translocation of calcium from cytoplasm to endoplasmic reticulum in a Ca2+-dependent manner. ACA2 contains an N-terminal autoinhibitory region. Truncation of this region constitutively activates ACA2. This region is also a calmodulin binding site, and thus binding of calmodulin to this region releases the autoinhibition.

Autoinhibitory domains (AIDs)

20-31 (N-terminal autoinhibitory region) EV

Target domain

434-805 (ATPase domain)

Relief mechanism

Partner binding

Assay

Deletion assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for O81108

Entry ID Method Resolution Chain Position Source
2M7E NMR - A 20-45 PDB
AF-O81108-F1 Predicted AlphaFoldDB

24 variants for O81108

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH06844755 48 Y>F No 1000Genomes
ENSVATH06844750 84 T>N No 1000Genomes
ENSVATH12465848 136 T>I No 1000Genomes
tmp_4_17686039_C_G 187 V>L No 1000Genomes
ENSVATH02972989 244 G>E No 1000Genomes
tmp_4_17685495_G_A 368 A>V No 1000Genomes
ENSVATH14353192 478 V>L No 1000Genomes
ENSVATH06844746 543 R>G No 1000Genomes
tmp_4_17684703_T_C 632 E>G No 1000Genomes
tmp_4_17684620_G_A 660 P>S No 1000Genomes
tmp_4_17684471_G_T 709 F>L No 1000Genomes
tmp_4_17684469_C_T 710 R>K No 1000Genomes
tmp_4_17684215_T_C 768 I>V No 1000Genomes
ENSVATH06844739 796 I>V No 1000Genomes
tmp_4_17683841_C_T 837 S>N No 1000Genomes
ENSVATH12465813 879 G>S No 1000Genomes
ENSVATH06844736 900 V>I No 1000Genomes
ENSVATH02972984 909 K>R No 1000Genomes
tmp_4_17683611_G_T 914 L>I No 1000Genomes
ENSVATH02972982 922 M>I No 1000Genomes
ENSVATH02972983 922 M>K No 1000Genomes
ENSVATH02972977 973 I>L No 1000Genomes
ENSVATH02972977 973 I>V No 1000Genomes
tmp_4_17683284_T_A 996 I>F No 1000Genomes

No associated diseases with O81108

5 regional properties for O81108

Type Name Position InterPro Accession
domain Cation-transporting P-type ATPase, N-terminal 116 - 189 IPR004014
domain Cation-transporting P-type ATPase, C-terminal 838 - 1011 IPR006068
ptm P-type ATPase, phosphorylation site 454 - 460 IPR018303
domain Calcium-transporting P-type ATPase, N-terminal autoinhibitory domain 5 - 49 IPR024750
domain P-type ATPase, haloacid dehalogenase domain 434 - 805 IPR044492

Functions

Description
EC Number 7.2.2.10 Linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Endoplasmic reticulum membrane; Multi-pass membrane protein
PANTHER Family PTHR24093 CATION TRANSPORTING ATPASE
PANTHER Subfamily PTHR24093:SF534 CALCIUM-TRANSPORTING ATPASE 2, PLASMA MEMBRANE-TYPE
PANTHER Protein Class primary active transporter
transporter
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
endoplasmic reticulum membrane The lipid bilayer surrounding the endoplasmic reticulum.
integral component of plasma membrane The component of the plasma membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
intracellular membrane-bounded organelle Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATPase-coupled cation transmembrane transporter activity Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + cation(out) = ADP + phosphate + cation(in).
calcium ion transmembrane transporter activity Enables the transfer of calcium (Ca) ions from one side of a membrane to the other.
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
metal ion binding Binding to a metal ion.
P-type calcium transporter activity Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + Ca2+(in) = ADP + phosphate + Ca2+(out).

1 GO annotations of biological process

Name Definition
calcium ion transmembrane transport A process in which a calcium ion is transported from one side of a membrane to the other by means of some agent such as a transporter or pore.

30 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38929 PMC1 Calcium-transporting ATPase 2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
D3K0R6 ATP2B4 Plasma membrane calcium-transporting ATPase 4 Bos taurus (Bovine) SS
Q01814 ATP2B2 Plasma membrane calcium-transporting ATPase 2 Homo sapiens (Human) SS
P23634 ATP2B4 Plasma membrane calcium-transporting ATPase 4 Homo sapiens (Human) EV
P20020 ATP2B1 Plasma membrane calcium-transporting ATPase 1 Homo sapiens (Human) SS
Q16720 ATP2B3 Plasma membrane calcium-transporting ATPase 3 Homo sapiens (Human) EV
G5E829 Atp2b1 Plasma membrane calcium-transporting ATPase 1 Mus musculus (Mouse) SS
Q9R0K7 Atp2b2 Plasma membrane calcium-transporting ATPase 2 Mus musculus (Mouse) SS
Q6Q477 Atp2b4 Plasma membrane calcium-transporting ATPase 4 Mus musculus (Mouse) SS
P23220 ATP2B1 Plasma membrane calcium-transporting ATPase 1 Sus scrofa (Pig) SS
P11506 Atp2b2 Plasma membrane calcium-transporting ATPase 2 Rattus norvegicus (Rat) SS
Q64542 Atp2b4 Plasma membrane calcium-transporting ATPase 4 Rattus norvegicus (Rat) SS
P11505 Atp2b1 Plasma membrane calcium-transporting ATPase 1 Rattus norvegicus (Rat) SS
Q64568 Atp2b3 Plasma membrane calcium-transporting ATPase 3 Rattus norvegicus (Rat) SS
Q8RUN1 ACA1 Calcium-transporting ATPase 1, plasma membrane-type Oryza sativa subsp japonica (Rice) SS
Q7X8B5 ACA5 Calcium-transporting ATPase 5, plasma membrane-type Oryza sativa subsp japonica (Rice) SS
Q65X71 ACA6 Probable calcium-transporting ATPase 6, plasma membrane-type Oryza sativa subsp japonica (Rice) SS
Q7XEK4 ACA7 Calcium-transporting ATPase 7, plasma membrane-type Oryza sativa subsp japonica (Rice) EV
Q2QY12 ACA9 Probable calcium-transporting ATPase 9, plasma membrane-type Oryza sativa subsp japonica (Rice) SS
Q2QMX9 ACA10 Calcium-transporting ATPase 10, plasma membrane-type Oryza sativa subsp japonica (Rice) SS
Q6ATV4 ACA3 Calcium-transporting ATPase 3, plasma membrane-type Oryza sativa subsp japonica (Rice) SS
O22218 ACA4 Calcium-transporting ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
O64806 ACA7 Putative calcium-transporting ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LF79 ACA8 Calcium-transporting ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
Q9LIK7 ACA13 Putative calcium-transporting ATPase 13, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LU41 ACA9 Calcium-transporting ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY77 ACA12 Calcium-transporting ATPase 12, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2L4 ACA11 Putative calcium-transporting ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SZR1 ACA10 Calcium-transporting ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q37145 ACA1 Calcium-transporting ATPase 1 Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MESYLNENFD VKAKHSSEEV LEKWRNLCGV VKNPKRRFRF TANLSKRYEA AAMRRTNQEK
70 80 90 100 110 120
LRIAVLVSKA AFQFISGVSP SDYTVPEDVK AAGFEICADE LGSIVESHDV KKLKFHGGVD
130 140 150 160 170 180
GLAGKLKASP TDGLSTEAAQ LSQRQELFGI NKFAESEMRG FWVFVWEALQ DMTLMILGVC
190 200 210 220 230 240
AFVSLIVGIA TEGWPKGSHD GLGIAASILL VVFVTATSDY RQSLQFRDLD KEKKKITVQV
250 260 270 280 290 300
TRNGFRQKLS IYDLLPGDIV HLAIGDQVPA DGLFLSGFSV VIDESSLTGE SEPVMVNAQN
310 320 330 340 350 360
PFLMSGTKVQ DGSCKMMITT VGMRTQWGKL MATLTEGGDD ETPLQVKLNG VATIIGKIGL
370 380 390 400 410 420
FFAVVTFAVL VQGMFMRKLS TGTHWVWSGD EALELLEYFA IAVTIVVVAV PEGLPLAVTL
430 440 450 460 470 480
SLAFAMKKMM NDKALVRHLA ACETMGSATT ICSDKTGTLT TNHMTVVKSC ICMNVQDVAN
490 500 510 520 530 540
KGSSLQSEIP ESAVKLLIQS IFNNTGGEVV VNKHGKTELL GTPTETAILE LGLSLGGKFQ
550 560 570 580 590 600
EERKSYKVIK VEPFNSTKKR MGVVIELPEG GRMRAHTKGA SEIVLAACDK VVNSSGEVVP
610 620 630 640 650 660
LDEESIKYLN VTINEFANEA LRTLCLAYMD IEGGFSPDDA IPASGFTCVG IVGIKDPVRP
670 680 690 700 710 720
GVKESVELCR RAGITVRMVT GDNINTAKAI ARECGILTDD GIAIEGPVFR EKNQEELLEL
730 740 750 760 770 780
IPKIQVMARS SPMDKHTLVK QLRTTFDEVV AVTGDGTNDA PALHEADIGL AMGIAGTEVA
790 800 810 820 830 840
KESADVIILD DNFSTIVTVA KWGRSVYINI QKFVQFQLTV NVVALVVNFS SACLTGSAPL
850 860 870 880 890 900
TAVQLLWVNM IMDTLGALAL ATEPPNDELM KRLPVGRRGN FITNAMWRNI LGQAVYQFIV
910 920 930 940 950 960
IWILQAKGKA MFGLDGPDST LMLNTLIFNC FVFCQVFNEI SSREMEEIDV FKGILDNYVF
970 980 990 1000 1010
VVVIGATVFF QIIIIEFLGT FASTTPLTIT QWIFSIFIGF LGMPIAAGLK TIPV