O77746
SS
Gene name |
PDE5A (PDE5) |
Protein name |
cGMP-specific 3',5'-cyclic phosphodiesterase |
Names |
cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE |
Species |
Canis lupus familiaris (Dog) (Canis familiaris) |
KEGG Pathway |
cfa:403825 |
EC number |
3.1.4.35: Phosphoric diester hydrolases |
Protein Class |
|
Descriptions
PDE5A plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE5A contains a catalytic domain that hydrolyzes cGMP and a regulatory (R) domain (1-539) that contains two GAFs, a and b. The R domain binds cGMP allosterically, provides for dimerization, and is phosphorylated at a site regulated by allosteric cGMP binding. The sequence containing GAF b and its flanking amino acids (403-539) autoinhibits GAF a cGMP-binding affinity.
Autoinhibitory domains (AIDs)
Target domain |
154-314 (GAF a domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
References
- Zoraghi R et al. (2005) "Structural and functional features in human PDE5A1 regulatory domain that provide for allosteric cGMP binding, dimerization, and regulation", The Journal of biological chemistry, 280, 12051-63
- Francis SH et al. (2011) "Mammalian cyclic nucleotide phosphodiesterases: molecular mechanisms and physiological functions", Physiological reviews, 91, 651-90
Autoinhibited structure
Activated structure
1 structures for O77746
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O77746-F1 | Predicted | AlphaFoldDB |
No variants for O77746
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O77746 | |||||
No associated diseases with O77746
4 regional properties for O77746
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain | 526 - 850 | IPR002073 |
| domain | GAF domain | 154 - 314 | IPR003018-1 |
| domain | GAF domain | 336 - 503 | IPR003018-2 |
| domain | HD/PDEase domain | 600 - 777 | IPR003607 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.1.4.35 | Phosphoric diester hydrolases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
1 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| 3',5'-cyclic-GMP phosphodiesterase activity | Catalysis of the reaction: 3',5'-cyclic GMP + H2O = GMP + H+. |
| 3',5'-cyclic-nucleotide phosphodiesterase activity | Catalysis of the reaction: a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate. |
| cGMP binding | Binding to cGMP, the nucleotide cyclic GMP (guanosine 3',5'-cyclophosphate). |
| metal ion binding | Binding to a metal ion. |
2 GO annotations of biological process
| Name | Definition |
|---|---|
| cGMP catabolic process | The chemical reactions and pathways resulting in the breakdown of cyclic GMP, guanosine 3',5'-phosphate. |
| signal transduction | The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. |
6 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q28156 | PDE5A | cGMP-specific 3',5'-cyclic phosphodiesterase | Bos taurus (Bovine) | SS |
| P33726 | PDE6B | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta | Canis lupus familiaris (Dog) (Canis familiaris) | PR |
| Q9VJ79 | Pde11 | Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11 | Drosophila melanogaster (Fruit fly) | SS |
| O76074 | PDE5A | cGMP-specific 3',5'-cyclic phosphodiesterase | Homo sapiens (Human) | EV |
| Q8CG03 | Pde5a | cGMP-specific 3',5'-cyclic phosphodiesterase | Mus musculus (Mouse) | SS |
| O54735 | Pde5a | cGMP-specific 3',5'-cyclic phosphodiesterase | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MERGSPGAGA | ARLPRDQDSV | EAWLDDHRDF | TFSYFVKKAT | REMVNAWFAE | RVHTIPVCKE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| GIRGHAESCS | CSSQQSSRAD | SSAPGTPTRK | ISASEFDRPL | RPIVVKDSEG | TVSFLADSEK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| KEQMPLTPPR | FDNDEGDQCS | RLLELVKDIS | SHLDVTALCH | KIFLHIHGLI | SADRYSLFLV |
| 190 | 200 | 210 | 220 | 230 | 240 |
| CEDSSNDKFL | ISRLFDVAEG | STLEEASNNC | IRLEWNKGIV | GHVAALGEPL | NIKDAYEDPR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| FNAEVDQITG | YKTQSILCMP | IKNHREEVVG | VAQAINKKSG | NGGTFTEKDE | KDFAAYLAFC |
| 310 | 320 | 330 | 340 | 350 | 360 |
| GIVLHNAQLY | ETSLLENKRN | QVLLDLASLI | FEEQQSLEVI | LKKIAATIIS | FMQVQKCTIF |
| 370 | 380 | 390 | 400 | 410 | 420 |
| IVDEDCSDSF | SSVFHMECEE | LEKLPDTLTR | ERDANRINYM | YAQYVKNTME | PLNIPDVSKD |
| 430 | 440 | 450 | 460 | 470 | 480 |
| KRFPWTNENT | GNVNQQCIRS | LLCTPIKNGK | KNKVIGVCQL | VNKMEENTGK | VKPFNRNDEQ |
| 490 | 500 | 510 | 520 | 530 | 540 |
| FLEAFVIFCG | LGIQNTQMYE | AVERAMAKQM | VTLEVLSYHA | SAAEEETKEL | QSLAAAVVPS |
| 550 | 560 | 570 | 580 | 590 | 600 |
| AQTLKITDFS | FSDFELSDLE | TALCTIRMFT | DLNLVQNFQM | KHEVLCRWIL | SVKKNYRKNV |
| 610 | 620 | 630 | 640 | 650 | 660 |
| AYHNWRHAFN | TAQCMFAALK | AGKIQNKLTD | LEILALLIAA | LSHDLDHRGV | NNSYIQRSEH |
| 670 | 680 | 690 | 700 | 710 | 720 |
| PLAQLYCHSI | MEHHHFDQCL | MILNSPGNQI | LSGLSIEEYK | TTLKIIKQAI | LATDLALYIK |
| 730 | 740 | 750 | 760 | 770 | 780 |
| RRGEFFELIR | KNQFNLEDPH | QKELFLAMLM | TACDLSAITK | PWPIQQRIAE | LVATEFFDQG |
| 790 | 800 | 810 | 820 | 830 | 840 |
| DRERKELNIE | PADLMNREKK | NKIPSMQVGF | IDAICLQLYE | ALTHVSEDCF | PLLDGCRKNR |
| 850 | 860 | ||||
| QKWQALAEQQ | EKTLINGESS | QAKRN |