O76924
SS
Gene name |
ari-2 (ari2, TRIAD1, CG5709) |
Protein name |
Potential E3 ubiquitin-protein ligase ariadne-2 |
Names |
EC 2.3.2.31 , Protein ariadne-2 , RING-type E3 ubiquitin transferase ariadne-2 |
Species |
Drosophila melanogaster (Fruit fly) |
KEGG Pathway |
dme:Dmel_CG5709 |
EC number |
2.3.2.31: Aminoacyltransferases |
Protein Class |
|
Descriptions
E3 ubiquitin-protein ligase ARIH2 catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 and acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex and initiating ubiquitination of ECS substrates. ARIH2 is autoinhibited by the ariadne domain, which masks the second RING-type zinc finger that contains the active site and inhibits the E3 activity. Inhibition is relieved upon binding to neddylated cullin-RING ubiquitin ligase complexes, which activate the E3 ligase activity.
Autoinhibitory domains (AIDs)
Target domain |
314-354 (RING2 domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Kelsall IR et al. (2013) "TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes", The EMBO journal, 32, 2848-60
- Kostrhon S et al. (2021) "CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific NEDD8 activation", Nature chemical biology, 17, 1075-1083
Autoinhibited structure
Activated structure
1 structures for O76924
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O76924-F1 | Predicted | AlphaFoldDB |
No variants for O76924
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O76924 | |||||
No associated diseases with O76924
8 regional properties for O76924
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Zinc finger, RING-type | 314 - 354 | IPR001841 |
| domain | IBR domain | 222 - 284 | IPR002867-1 |
| domain | IBR domain | 292 - 354 | IPR002867-2 |
| conserved_site | Zinc finger, RING-type, conserved site | 332 - 341 | IPR017907 |
| domain | TRIAD supradomain | 149 - 358 | IPR044066 |
| domain | Ariadne domain | 371 - 477 | IPR045840 |
| domain | E3 ubiquitin-protein ligase ARIH2, BRcat domain | 231 - 302 | IPR047555 |
| domain | E3 ubiquitin-protein ligase TRIAD1, Rcat domain | 306 - 361 | IPR047556 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.3.2.31 | Aminoacyltransferases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| ubiquitin ligase complex | A protein complex that includes a ubiquitin-protein ligase and enables ubiquitin protein ligase activity. The complex also contains other proteins that may confer substrate specificity on the complex. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| ubiquitin conjugating enzyme binding | Binding to a ubiquitin conjugating enzyme, any of the E2 proteins. |
| ubiquitin protein ligase activity | Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S -> X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond |
| ubiquitin-protein transferase activity | Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages. |
| zinc ion binding | Binding to a zinc ion (Zn). |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| positive regulation of proteasomal ubiquitin-dependent protein catabolic process | Any process that activates or increases the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome. |
| protein polyubiquitination | Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain. |
| ubiquitin-dependent protein catabolic process | The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein. |
13 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A2VEA3 | ARIH1 | E3 ubiquitin-protein ligase ARIH1 | Bos taurus (Bovine) | SS |
| Q7KTX7 | park | E3 ubiquitin-protein ligase parkin | Drosophila melanogaster (Fruit fly) | SS |
| Q94981 | ari-1 | E3 ubiquitin-protein ligase ariadne-1 | Drosophila melanogaster (Fruit fly) | SS |
| Q9NWF9 | RNF216 | E3 ubiquitin-protein ligase RNF216 | Homo sapiens (Human) | PR |
| O95376 | ARIH2 | E3 ubiquitin-protein ligase ARIH2 | Homo sapiens (Human) | EV |
| Q9Y4X5 | ARIH1 | E3 ubiquitin-protein ligase ARIH1 | Homo sapiens (Human) | EV |
| Q9Z1K6 | Arih2 | E3 ubiquitin-protein ligase ARIH2 | Mus musculus (Mouse) | SS |
| Q9Z1K5 | Arih1 | E3 ubiquitin-protein ligase ARIH1 | Mus musculus (Mouse) | SS |
| Q22431 | ari-2 | Potential E3 ubiquitin-protein ligase ariadne-2 | Caenorhabditis elegans | SS |
| O01965 | ari-1.1 | E3 ubiquitin-protein ligase ari-1.1 | Caenorhabditis elegans | SS |
| B1H1E4 | arih1 | E3 ubiquitin-protein ligase arih1 | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| Q6PFJ9 | arih1 | E3 ubiquitin-protein ligase arih1 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| Q6NW85 | arih1l | E3 ubiquitin-protein ligase arih1l | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MDSDIEMDME | SDNDGEYDDD | YDYYNTGEDC | DVERLDPKRA | DPEYFEYECL | TVEDIEKLLN |
| 70 | 80 | 90 | 100 | 110 | 120 |
| ERVEKLNTIL | QITPSLAKVL | LLEHQWNNVA | VVEKYRQDAN | ALLVTARIKP | PSVAVTDTAS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| TSAAAASAQL | LRLGSSGYKT | TASATPQYRS | QMCPVCASSQ | LGDKFYSLAC | GHSFCKDCWT |
| 190 | 200 | 210 | 220 | 230 | 240 |
| IYFETQIFQG | ISTQIGCMAQ | MCNVRVPEDL | VLTLVTRPVM | RDKYQQFAFK | DYVKSHPELR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| FCPGPNCQII | VQSSEISAKR | AICKACHTGF | CFRCGMDYHA | PTDCQVIKKW | LTKCADDSET |
| 310 | 320 | 330 | 340 | 350 | 360 |
| ANYISAHTKD | CPKCHICIEK | NGGCNHMQCF | NCKHDFCWMC | LGDWKTHGSE | YYECSRYKDN |
| 370 | 380 | 390 | 400 | 410 | 420 |
| PNIANESVHV | QAREALKKYL | HYYERWENHS | KSLKLEQQTI | DRLRQRINSK | VMNGSGTWID |
| 430 | 440 | 450 | 460 | 470 | 480 |
| WQYLFNAAAL | LAKCRYTLQY | TYPYAYYMEA | GSRKNLFEYQ | QAQLEAEIEN | LSWKIERAET |
| 490 | 500 | ||||
| TDLGDLENQM | DIAEKRRTTL | LKDFFPVDA |