O76360

PR
Gene name
egl-4
Protein name
cGMP-dependent protein kinase egl-4
Names
Egg-laying defective protein 4
Species
Caenorhabditis elegans
KEGG Pathway
EC number
2.7.11.12: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-52 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

610-632 (Activation loop from InterPro)

Target domain

469-729 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for O76360

Entry ID Method Resolution Chain Position Source
AF-O76360-F1 Predicted AlphaFoldDB

No variants for O76360

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O76360

No associated diseases with O76360

11 regional properties for O76360

Type Name Position InterPro Accession
domain Cyclic nucleotide-binding domain 201 - 316 IPR000595-1
domain Cyclic nucleotide-binding domain 319 - 442 IPR000595-2
domain Protein kinase domain 469 - 729 IPR000719
domain AGC-kinase, C-terminal 730 - 780 IPR000961
active_site Serine/threonine-protein kinase, active site 589 - 601 IPR008271
binding_site Protein kinase, ATP binding site 475 - 499 IPR017441
conserved_site Cyclic nucleotide-binding, conserved site 228 - 244 IPR018488-1
conserved_site Cyclic nucleotide-binding, conserved site 264 - 281 IPR018488-2
conserved_site Cyclic nucleotide-binding, conserved site 346 - 362 IPR018488-3
conserved_site Cyclic nucleotide-binding, conserved site 388 - 405 IPR018488-4
domain cGMP-dependent protein kinase, catalytic domain 475 - 736 IPR035014

Functions

Description
EC Number 2.7.11.12 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • In resting AWC sensory neurons, localizes in cytoplasm (PubMed:20220099, PubMed:23954825, PubMed:27383131)
  • Prolonged exposure to attractive odorants sensed by AWC neurons results in nuclear translocation (PubMed:20220099, PubMed:23954825, PubMed:27383131)
  • Nuclear translocation is required for the adaptation to prolonged odor exposure and is controlled by G(o)-alpha subunit protein goa-1 (PubMed:20220099, PubMed:23954825, PubMed:27383131)
  • Localization is regulated by cGMP levels: high cGMP levels result in cytoplasmic localization whereas low cGMP levels result in nuclear localization (PubMed:20220099, PubMed:22319638)
  • Nuclear localization in AWC neurons is dependent on age-1 (PubMed:27383131)
  • In addition, an intact sensory cilia structure is required for cytoplasmic localization in resting AWC neurons (PubMed:22319638)
  • In resting AWB sensory neurons, constitutive nuclear localization is dependent on goa-1 (PubMed:23954825)
  • In resting ASH sensory neurons, localizes in both cytoplasm and nucleus (PubMed:23874221)
  • Cytoplasmic localization is important for negative regulation of quinine sensitivity in ASH neurons (PubMed:23874221)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cGMP binding Binding to cGMP, the nucleotide cyclic GMP (guanosine 3',5'-cyclophosphate).
cGMP-dependent protein kinase activity cGMP dependent catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein.
metal ion binding Binding to a metal ion.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.

35 GO annotations of biological process

Name Definition
chemosensory behavior Behavior that is dependent upon the sensation of chemicals.
chemotaxis The directed movement of a motile cell or organism, or the directed growth of a cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis).
chemotropism The movement of an organism, or part of an organism, in response to an external chemical gradient, usually toward or away from it.
determination of adult lifespan The pathways that regulate the duration of the adult phase of the life-cycle of an animal.
insulin receptor signaling pathway The series of molecular signals generated as a consequence of the insulin receptor binding to insulin.
larval feeding behavior Feeding behavior in a larval (immature) organism.
negative regulation of calcium-mediated signaling Any process that stops, prevents, or reduces the frequency, rate or extent of calcium-mediated signaling.
negative regulation of cell growth Any process that stops, prevents, or reduces the frequency, rate, extent or direction of cell growth.
negative regulation of cGMP-mediated signaling Any process that decreases the rate, frequency or extent of cGMP-mediated signaling.
negative regulation of cyclic nucleotide-gated ion channel activity Any process that stops, prevents or reduces the frequency, rate or extent of cyclic nucleotide-gated ion channel activity.
negative regulation of dauer larval development Any process that decreases the rate, frequency, or extent of dauer larval development, the process whose specific outcome is the progression of the dauer larva over time, through the facultative diapause of the dauer (enduring) larval stage, with specialized traits adapted for dispersal and long-term survival, with elevated stress resistance and without feeding.
negative regulation of multicellular organism growth Any process that stops, prevents, or reduces the frequency, rate or extent of growth of an organism to reach its usual body size.
negative regulation of organ growth Any process that stops, prevents, or reduces the frequency, rate or extent of growth of an organ of an organism.
negative regulation of transforming growth factor beta receptor signaling pathway Any process that stops, prevents, or reduces the frequency, rate or extent of any TGF-beta receptor signaling pathway.
olfactory learning Any process in an organism in which a relatively long-lasting adaptive behavioral change occurs in response to (repeated) exposure to an olfactory cue.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
positive regulation of cellular response to alcohol Any process that activates or increases the frequency, rate or extent of cellular response to alcohol.
positive regulation of cGMP-mediated signaling Any process that increases the rate, frequency or extent of cGMP-mediated signaling.
positive regulation of chemotaxis Any process that activates or increases the frequency, rate or extent of the directed movement of a motile cell or organism in response to a specific chemical concentration gradient.
positive regulation of cyclic-nucleotide phosphodiesterase activity Any process that activates or increases the frequency, rate or extent of cyclic nucleotide phosphodiesterase activity, the catalysis of the reaction: nucleotide 3',5'-cyclic phosphate + H2O = nucleotide 5'-phosphate.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of oviposition Any process that activates or increases the frequency, rate or extent of oviposition.
protein phosphorylation The process of introducing a phosphate group on to a protein.
receptor guanylyl cyclase signaling pathway The series of molecular signals initiated by an extracellular ligand binding to a receptor on the surface of the target cell where the receptor possesses guanylyl cyclase activity, and ending with the regulation of a downstream cellular process, e.g. transcription.
regulation of eating behavior Any process that modulates the frequency, rate or extent of eating behavior.
regulation of gene expression Any process that modulates the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
regulation of multicellular organism growth Any process that modulates the frequency, rate or extent of growth of the body of an organism so that it reaches its usual body size.
regulation of oviposition Any process that modulates the frequency, rate or extent of the deposition of eggs, either fertilized or not, upon a surface or into a medium.
response to alcohol Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an alcohol stimulus.
response to hydrogen peroxide Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hydrogen peroxide (H2O2) stimulus.
response to odorant Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an odorant stimulus. An odorant is any substance capable of stimulating the sense of smell.
response to oxygen levels Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus reflecting the presence, absence, or concentration of oxygen.
sensory perception of bitter taste The series of events required to receive a bitter taste stimulus, convert it to a molecular signal, and recognize and characterize the signal. This is a neurological process.
signal transduction The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
sleep Any process in which an organism enters and maintains a periodic, readily reversible state of reduced awareness and metabolic activity. Usually accompanied by physical relaxation, the onset of sleep in humans and other mammals is marked by a change in the electrical activity of the brain.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P00516 PRKG1 cGMP-dependent protein kinase 1 Bos taurus (Bovine) SS
Q03043 for cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B Drosophila melanogaster (Fruit fly) SS
Q13237 PRKG2 cGMP-dependent protein kinase 2 Homo sapiens (Human) PR
Q13976 PRKG1 cGMP-dependent protein kinase 1 Homo sapiens (Human) EV
P0C605 Prkg1 cGMP-dependent protein kinase 1 Mus musculus (Mouse) SS
Q61410 Prkg2 cGMP-dependent protein kinase 2 Mus musculus (Mouse) PR
Q64595 Prkg2 cGMP-dependent protein kinase 2 Rattus norvegicus (Rat) PR
P21137 kin-1 cAMP-dependent protein kinase catalytic subunit Caenorhabditis elegans PR
Q7JP68 F47F2.1 cAMP-dependent protein kinase, catalytic subunit-like Caenorhabditis elegans PR
10 20 30 40 50 60
MSSGSRPSSG GGGGGGGASG GAGGGAPGGG GGGIRGFFSK LRKPSDQPNG NQVQVGTRTF
70 80 90 100 110 120
EAHELQKLIP QLEEAISRKD AQLRQQQTIV EGHIKRISEL EGEVTTLQRE CDKLRSVLEQ
130 140 150 160 170 180
KAQSAASPGG QPPSPSPRTD QLGNDLQQKA VLPADGVQRA KKIAVSAEPT NFENKPATLQ
190 200 210 220 230 240
HYNKTVGAKQ MIRDAVQKND FLKQLAKEQI IELVNCMYEM RARAGQWVIQ EGEPGDRLFV
250 260 270 280 290 300
VAEGELQVSR EGALLGKMRA GTVMGELAIL YNCTRTASVQ ALTDVQLWVL DRSVFQMITQ
310 320 330 340 350 360
RLGMERHSQL MNFLTKVSIF QNLSEDRISK MADVMDQDYY DGGHYIIRQG EKGDAFFVIN
370 380 390 400 410 420
SGQVKVTQQI EGETEPREIR VLNQGDFFGE RALLGEEVRT ANIIAQAPGV EVLTLDRESF
430 440 450 460 470 480
GKLIGDLESL KKDYGDKERL AQVVREPPSP VKIVDDFREE FAQVTLKNVK RLATLGVGGF
490 500 510 520 530 540
GRVELVCVNG DKAKTFALKA LKKKHIVDTR QQEHIFAERN IMMETSTDWI VKLYKTFRDQ
550 560 570 580 590 600
KFVYMLLEVC LGGELWTTLR DRGHFDDYTA RFYVACVLEG LEYLHRKNIV YRDLKPENCL
610 620 630 640 650 660
LANTGYLKLV DFGFAKKLAS GRKTWTFCGT PEYVSPEIIL NKGHDQAADY WALGIYICEL
670 680 690 700 710 720
MLGRPPFQAS DPMKTYTLIL KGVDALEIPN RRIGKTATAL VKKLCRDNPG ERLGSGSGGV
730 740 750 760 770
NDIRKHRWFM GFDWEGLRSR TLKPPILPKV SNPADVTNFD NYPPDNDVPP DEFSGWDEGF