AiPD: Autoinhibited Protein Database

O75351

Gene name	VPS4B
Protein name	Vacuolar protein sorting-associated protein 4B
Names	Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1
Species	Homo sapiens (Human)
KEGG Pathway	hsa:9525
EC number	3.6.4.6: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

(Annotation based on sequence homology with Q9UN37)
VPS4 proteins are AAA+ ATPases required to form multivesicular bodies, release viral particles, and complete cytokinesis. VPS4 proteins act by disassembling ESCRT-III heteropolymers during or after their proposed function in membrane scission. Deleting the N-terminal MIT domain and adjacent linker from VPS4A increases both basal and liposome-enhanced ATPase activity, indicating that these elements play a role in autoinhibiting VPS4A until it encounters ESCRT-III polymers. The interactions between acidic ESCRT-III residues and sequences in VPS4A, in particular in the linker connecting the MIT and AAA+ domains, are involved in regulating the intrinsic autoinhibition of the enzyme.

Autoinhibitory domains (AIDs)

54-144 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

54-144 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

8 structures for O75351

Entry ID	Method	Resolution	Chain	Position	Source
1WR0	NMR	-	A	1-77	PDB
1XWI	X-ray	280 A	A	123-444	PDB
2CPT	NMR	-	A	1-104	PDB
2JQH	NMR	-	A	1-86	PDB
2JQK	NMR	-	A	1-86	PDB
4U7Y	X-ray	250 A	A	1-89	PDB
7L9X	X-ray	281 A	A	1-444	PDB
AF-O75351-F1	Predicted				AlphaFoldDB

220 variants for O75351

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs775341025 CA8986651	3	S>F		No	ClinGen ExAC TOPMed gnomAD
rs373658563 CA301783721	4	T>I		No	ClinGen Ensembl
rs769719797 CA8986650	5	S>L	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
CA402642369 rs1200425372	6	P>T		No	ClinGen TOPMed
rs539290608 CA402642355	7	N>I		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA8986648 rs539290608	7	N>S		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs539290608 CA402642353	7	N>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA402642344 rs1369275222	8	L>F		No	ClinGen TOPMed
CA402641644 rs1348257237	11	A>V		No	ClinGen gnomAD
rs142770219 CA8986613	14	L>V		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA402641595 rs1364478574	16	S>G		No	ClinGen TOPMed gnomAD
CA301774908 rs889609154	17	K>T		No	ClinGen TOPMed gnomAD
TCGA novel	19	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1468344635 CA402641513	23	K>E		No	ClinGen gnomAD
rs77284385 CA301774903	23	K>M		No	ClinGen Ensembl
CA8986610 rs760728919	24	A>S		No	ClinGen ExAC gnomAD
CA402641500 rs1204784944	24	A>V		No	ClinGen TOPMed
CA301774881 rs1008460201	26	N>D		No	ClinGen TOPMed gnomAD
CA8986607 rs747766401	28	E>A		No	ClinGen ExAC gnomAD
COSM3821662 rs771753062 CA8986608	28	E>K	Variant assessed as Somatic; 0.0 impact. breast [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA TOPMed gnomAD
CA8986606 rs778733915	29	E>A		No	ClinGen ExAC TOPMed gnomAD
rs778733915 CA402641469	29	E>G		No	ClinGen ExAC TOPMed gnomAD
CA301774875 rs112622461	34	Y>H		No	ClinGen Ensembl
rs1284114900 CA402641420 COSM1324531	36	H>R	ovary Variant assessed as Somatic; 0.0 impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated NCI-TCGA gnomAD
CA402641409 rs1264129728	38	V>L		No	ClinGen gnomAD
rs1344431459 CA402641393	40	Y>C		No	ClinGen gnomAD
CA402641359 rs1317631687	45	V>I		No	ClinGen gnomAD
CA402641327 rs1183371398	47	Y>*		No	ClinGen gnomAD
CA402641313 rs1460310540	49	A>V		No	ClinGen gnomAD
CA8986583 rs746333097	56	Q>R		No	ClinGen ExAC gnomAD
CA8986582 rs781678987	57	S>N		No	ClinGen ExAC gnomAD
CA8986581 rs17688948 VAR_023385	58	I>M	induces thermal instability [UniProt]	No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
rs1233871143 CA402641238	60	A>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs1315570182 CA402641226	62	C>S		No	ClinGen gnomAD
CA402641224 rs1245148080	62	C>Y		No	ClinGen gnomAD
CA8986577 rs572345457	73	K>Q		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	75	Y>C	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs75197917 CA301773779	76	L>M		No	ClinGen ExAC gnomAD
rs750332149 CA8986574	78	N>K		No	ClinGen ExAC gnomAD
rs767570340 CA8986573	80	E>A		No	ClinGen ExAC gnomAD
CA8986570 rs763711021	83	A>T		No	ClinGen ExAC
rs932945721 CA301773751	83	A>V		No	ClinGen TOPMed
rs775240653 CA402641001 CA402641003	84	Q>H		No	ClinGen ExAC gnomAD
CA8986569 rs762482134	84	Q>K		No	ClinGen ExAC gnomAD
CA402641008 rs1568089202	84	Q>P		No	ClinGen Ensembl
rs1319824000 CA402640983	86	P>S		No	ClinGen gnomAD
CA8986565 rs532105479	90	G>R		No	ClinGen 1000Genomes ExAC gnomAD
CA8986564 rs560222347	92	P>L		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs747569589 CA8986562	93	S>N		No	ClinGen ExAC gnomAD
rs774790929 CA8986561	95	A>V	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
CA301771145 rs976152185	101	D>G		No	ClinGen TOPMed
rs771991314 CA8986542	102	S>R		No	ClinGen ExAC gnomAD
CA301771128 rs956770613	104	G>W		No	ClinGen TOPMed
TCGA novel	107	E>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	108	S>Y	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA402640413 rs1187082863	109	D>G		No	ClinGen gnomAD
rs1263202433 CA402640416	109	D>H		No	ClinGen gnomAD
CA402640391 rs1342965466	112	E>G		No	ClinGen TOPMed
rs1141024 CA8986540	114	K>R		No	ClinGen ExAC gnomAD
rs750318908	114	K>R	Variant assessed as Somatic; 4.719e-05 impact. [NCI-TCGA]	No	NCI-TCGA
rs1141024 CA402640376	114	K>T		No	ClinGen ExAC gnomAD
CA402640361 rs1205179492	116	L>P		No	ClinGen TOPMed gnomAD
rs1205274888 CA402640354	117	Q>P		No	ClinGen gnomAD
CA402640254 rs1382565047	122	G>V		No	ClinGen TOPMed gnomAD
rs775333614 CA301768340	123	A>V		No	ClinGen TOPMed gnomAD
rs202058899 CA8986520	126	I>M		No	ClinGen ESP ExAC TOPMed gnomAD
CA8986521 rs768899063	126	I>T		No	ClinGen ExAC TOPMed gnomAD
CA402640221 rs1387781185	128	R>*	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
rs373315578 CA402640220	128	R>P		No	ClinGen ESP gnomAD
COSM3422270 rs373315578 CA301768324	128	R>Q	Variant assessed as Somatic; 0.0 impact. large_intestine [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ESP NCI-TCGA gnomAD
rs1445149607 CA402640216	129	P>T		No	ClinGen gnomAD
rs1367720947 CA402640208	130	N>S		No	ClinGen gnomAD
CA8986519 rs780446635	131	V>G		No	ClinGen ExAC gnomAD
rs770792561 CA8986518	132	K>R		No	ClinGen ExAC TOPMed gnomAD
rs777493409 CA8986516	133	W>*		No	ClinGen ExAC gnomAD
CA402640177 rs758341819	134	S>R		No	ClinGen ExAC gnomAD
CA8986514 rs201158795	135	D>V		No	ClinGen ExAC gnomAD
rs142699030 CA8986512 COSM438308	136	V>I	Variant assessed as Somatic; 0.0 impact. breast [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated 1000Genomes ExAC NCI-TCGA gnomAD
rs753435564 CA8986511	137	A>V		No	ClinGen ExAC gnomAD
rs983145250 CA301768302	139	L>H		No	ClinGen gnomAD
rs766028023 CA8986510	140	E>Q		No	ClinGen ExAC gnomAD
CA402640118 rs1408828233	144	E>A		No	ClinGen gnomAD
CA402640107 rs1304398410	146	L>M		No	ClinGen gnomAD
rs969107427 CA301768294	150	V>G		No	ClinGen Ensembl
rs148042910 CA8986509	150	V>M		No	ClinGen ESP ExAC TOPMed gnomAD
CA402640069 rs1367238668	152	L>M		No	ClinGen TOPMed
TCGA novel	153	P>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA402640049 rs1455212232	155	K>Q		No	ClinGen gnomAD
CA402640042 rs1302362203	156	F>L		No	ClinGen TOPMed
CA8986505 rs775031234	158	H>R		No	ClinGen ExAC gnomAD
CA8986504 rs768807383	159	L>V		No	ClinGen ExAC gnomAD
rs752055600 CA8986486	165	T>A		No	ClinGen ExAC gnomAD
TCGA novel	174	G>W	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs910450361 CA301766256	175	P>L	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
CA8986479 rs771848778	182	Y>C		No	ClinGen ExAC TOPMed gnomAD
rs1369809114 CA402639859	182	Y>H		No	ClinGen TOPMed
rs1319658987 CA402639839	185	K>E		No	ClinGen gnomAD
CA402639815 rs1166762346	188	A>V		No	ClinGen TOPMed
rs747947691 CA8986478	189	T>I		No	ClinGen ExAC TOPMed gnomAD
CA402639811 rs747947691	189	T>R		No	ClinGen ExAC TOPMed gnomAD
COSM78767 rs1408021325 CA402639788	192	N>K	ovary [Cosmic]	No	ClinGen cosmic curated gnomAD
CA8986477 rs774202246	193	N>D		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	194	S>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA8986476 rs768570547	194	S>L		No	ClinGen ExAC gnomAD
rs369884834	198	S>F	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	198	S>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs960455655 CA301766229	199	I>T		No	ClinGen TOPMed gnomAD
CA8986475 rs772659865	199	I>V		No	ClinGen ExAC gnomAD
CA402639690 rs755611326	204	L>I		No	ClinGen ExAC TOPMed gnomAD
rs755611326 CA8986473	204	L>V		No	ClinGen ExAC TOPMed gnomAD
CA402639666 rs1342434212	206	S>C		No	ClinGen TOPMed
CA402639655 rs1462989562	207	K>R		No	ClinGen gnomAD
TCGA novel	213	E>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs749187337 CA8986456	214	K>N		No	ClinGen ExAC TOPMed gnomAD
CA402639576 rs1383576196	214	K>R		No	ClinGen TOPMed gnomAD
CA402639556 rs1206175838	215	L>Q		No	ClinGen gnomAD
CA402639544 rs1568085412	217	K>M		No	ClinGen Ensembl
CA402639517 rs1202987143	221	Q>E		No	ClinGen gnomAD
rs1161902773 CA402639506	222	L>P		No	ClinGen TOPMed
rs745375968 CA8986453	225	E>D		No	ClinGen ExAC gnomAD
rs1377257364 CA402639461	229	S>P		No	ClinGen gnomAD
CA8986452 rs780823682	233	I>V		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	236	I>M	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA8986451 rs149336699	240	C>Y		No	ClinGen ESP ExAC gnomAD
rs746705982 CA8986450	241	G>D		No	ClinGen ExAC
TCGA novel	246	N>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA402639305 rs138933226	251	A>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs138933226 CA8986445	251	A>T		No	ClinGen ESP ExAC TOPMed gnomAD
rs372889875 CA8986444	252	R>C		No	ClinGen ESP ExAC TOPMed gnomAD
rs753852708 COSM438306 CA8986443	252	R>H	Variant assessed as Somatic; 0.0 impact. breast [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA gnomAD
CA402639270 rs1178624815	256	T>M		No	ClinGen TOPMed gnomAD
TCGA novel	258	F>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA402639228 rs1244309366	262	M>I		No	ClinGen TOPMed
rs756212918 CA8986417	264	G>A		No	ClinGen ExAC gnomAD
rs1176173997 CA402639188	267	V>I		No	ClinGen TOPMed
rs752422242 CA8986413	269	N>S		No	ClinGen ExAC gnomAD
CA301765207 rs912112346	270	D>N		No	ClinGen TOPMed gnomAD
rs1414070200 CA402639135	274	V>A		No	ClinGen Ensembl
rs1599357886 CA402639131	275	L>P		No	ClinGen Ensembl
CA8986410 rs371815146	280	I>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs895709121 CA301765197	280	I>V		No	ClinGen Ensembl
rs770516367 CA8986409	283	V>I		No	ClinGen ExAC gnomAD
CA402639067 rs770516367	283	V>L		No	ClinGen ExAC gnomAD
rs760270615 CA8986408	288	I>M		No	ClinGen ExAC gnomAD
rs760759039 CA8986391	295	R>Q	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
rs772832718 CA8986390	301	P>L		No	ClinGen ExAC gnomAD
CA8986388 rs761335205	303	P>L		No	ClinGen ExAC gnomAD
CA402638922 rs1371855334	303	P>S		No	ClinGen gnomAD
rs768849402 CA8986386	304	H>R		No	ClinGen ExAC TOPMed gnomAD
rs1189302789 CA402638910	305	A>S		No	ClinGen gnomAD
rs1424631496 CA402638907	305	A>V		No	ClinGen TOPMed gnomAD
CA8986385 rs749668835	306	R>*		No	ClinGen ExAC gnomAD
CA8986384 rs775900762	308	A>V		No	ClinGen ExAC gnomAD
rs1275420534 CA402638886 CA402638885	309	M>I		No	ClinGen gnomAD
rs531693634 CA301763205	309	M>T		No	ClinGen gnomAD
CA402638890 rs1599356735	309	M>V		No	ClinGen Ensembl
CA402638865 rs1205129731	312	L>P		No	ClinGen gnomAD
rs980286737 CA301763193	315	G>E		No	ClinGen TOPMed gnomAD
rs776365854 COSM989460 CA301763175	322	T>M	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated NCI-TCGA TOPMed gnomAD
rs757387117 CA8986380	323	E>D		No	ClinGen ExAC gnomAD
rs1308071182 CA402638789	324	A>T		No	ClinGen TOPMed gnomAD
rs747150958 CA8986379	325	D>E		No	ClinGen ExAC TOPMed gnomAD
CA8986376 rs753679036	327	R>Q		No	ClinGen ExAC gnomAD
CA8986377 rs150331543	327	R>W	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ESP ExAC NCI-TCGA TOPMed gnomAD
rs752058625 CA301763079	341	I>K		No	ClinGen gnomAD
CA402638641 rs1234832861	346	R>C		No	ClinGen TOPMed
rs1202244873 CA402638638	346	R>H		No	ClinGen TOPMed gnomAD
CA402638615 rs1264015297	350	M>L		No	ClinGen gnomAD
CA8986369 rs539348167	353	V>I		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA402638573 rs1408619377	356	V>L		No	ClinGen gnomAD
rs140180968 CA8986367	360	T>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA402638539 rs1325512539	361	H>R		No	ClinGen gnomAD
CA402638541 rs1185922964	361	H>Y		No	ClinGen TOPMed
CA8986329 rs200235369 COSM1711456	366	R>C	skin [Cosmic]	No	ClinGen cosmic curated 1000Genomes ExAC TOPMed gnomAD
rs952229759 CA301760094	366	R>H		No	ClinGen TOPMed gnomAD
CA402638489 rs1414046021 COSM564097	367	G>R	lung Variant assessed as Somatic; 0.0 impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated NCI-TCGA gnomAD
TCGA novel	368	P>H	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA8986326 rs369063057	368	P>S		No	ClinGen ESP ExAC gnomAD
CA402638473 rs1171095126	369	S>F		No	ClinGen gnomAD
CA8986324 rs757925293	370	R>*		No	ClinGen ExAC gnomAD
rs147130607 CA8986323	370	R>L		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA8986322 rs147130607 COSM264665	370	R>Q	large_intestine [Cosmic]	No	ClinGen cosmic curated 1000Genomes ExAC TOPMed gnomAD
CA402638454 rs1474232084	373	P>S		No	ClinGen gnomAD
rs1238908521 CA402638446	374	N>S		No	ClinGen gnomAD
CA8986320 rs754213173	375	H>Y		No	ClinGen ExAC gnomAD
rs142404608 CA8986319	376	L>F		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs965934959 CA301760016	377	V>I		No	ClinGen TOPMed gnomAD
rs773617268 CA8986317	380	L>P		No	ClinGen ExAC gnomAD
rs761210420 CA8986318	380	L>V		No	ClinGen ExAC gnomAD
rs201406128 CA8986316	381	L>V		No	ClinGen ExAC TOPMed gnomAD
rs553400769 CA8986315	382	T>I		No	ClinGen 1000Genomes ExAC gnomAD
CA301759949 rs1033915095	385	S>C		No	ClinGen TOPMed
CA8986313 rs140107443	385	S>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1447548931 CA402638379	386	P>S		No	ClinGen gnomAD
CA402638372 rs1271988113	387	G>R		No	ClinGen TOPMed
rs1205435046 CA402638358	389	P>A		No	ClinGen TOPMed
rs146639296 CA8986311	392	I>V		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA301759891 rs112699327	400	P>S		No	ClinGen Ensembl
TCGA novel	403	K>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs746914721 CA8986309	408	V>F		No	ClinGen ExAC TOPMed gnomAD
CA8986308 rs144476720	410	S>A		No	ClinGen ESP ExAC TOPMed gnomAD
CA301759867 rs747235022	411	M>L		No	ClinGen gnomAD
rs1010609426 CA301759860	411	M>T		No	ClinGen TOPMed gnomAD
rs139544128 CA402638187	412	S>*		No	ClinGen ESP ExAC TOPMed gnomAD
rs139544128 CA8986288	412	S>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs765072823 CA301757364	416	R>Q		No	ClinGen gnomAD
CA301757359 rs1022701364	418	L>V		No	ClinGen TOPMed gnomAD
CA8986286 rs748129460	420	N>K		No	ClinGen ExAC gnomAD
CA402638134 rs1293312191	421	T>A		No	ClinGen gnomAD
TCGA novel	423	P>H	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA8986284 rs754512421	425	V>I		No	ClinGen ExAC TOPMed gnomAD
rs748915944 CA8986283	426	N>D		No	ClinGen ExAC
CA402638102 rs1322194152	426	N>S		No	ClinGen TOPMed gnomAD
rs1011747661 CA301757341	427	E>K		No	ClinGen TOPMed
TCGA novel	429	D>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1207854390 CA402638020	437	T>A		No	ClinGen TOPMed
CA402637988 rs1174587685	441	G>D		No	ClinGen gnomAD
CA8986281 rs755866565	442	Q>*		No	ClinGen ExAC gnomAD
CA8986279 rs768043383	444	G>A		No	ClinGen ExAC TOPMed gnomAD
CA8986280 rs768043383	444	G>D		No	ClinGen ExAC TOPMed gnomAD

No associated diseases with O75351

No regional properties for O75351

Type	Name	Position	InterPro Accession
No domain, repeats, and functional sites for O75351

Functions

		Description
EC Number	3.6.4.6	Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization	Late endosome membrane ; Peripheral membrane protein	Membrane-associated in the prevacuolar endosomal compartment Localized in HIV-1 particles purified from acutely infected cells
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

14 GO annotations of cellular component

Name	Definition
ATPase complex	A protein complex which is capable of ATPase activity.
centrosome	A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endosome	A vacuole to which materials ingested by endocytosis are delivered.
endosome membrane	The lipid bilayer surrounding an endosome.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
Flemming body	A cell part that is the central region of the midbody characterized by a gap in alpha-tubulin staining. It is a dense structure of antiparallel microtubules from the central spindle in the middle of the intercellular bridge.
late endosome membrane	The lipid bilayer surrounding a late endosome.
midbody	A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nuclear pore	A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
spindle pole	Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

6 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity	Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
identical protein binding	Binding to an identical protein or proteins.
protein C-terminus binding	Binding to a protein C-terminus, the end of a peptide chain at which the 1-carboxyl function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
protein homodimerization activity	Binding to an identical protein to form a homodimer.
protein-containing complex binding	Binding to a macromolecular complex.

32 GO annotations of biological process

Name	Definition
autophagosome maturation	Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling.
autophagy	The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation.
cholesterol transport	The directed movement of cholesterol, cholest-5-en-3-beta-ol, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
endosomal transport	The directed movement of substances mediated by an endosome, a membrane-bounded organelle that carries materials enclosed in the lumen or located in the endosomal membrane.
endosome to lysosome transport via multivesicular body sorting pathway	The directed movement of substances from endosomes to lysosomes by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the lysosome.
ESCRT III complex disassembly	The disaggregation of an ESCRT III complex into its constituent components.
late endosomal microautophagy	The autophagy process by which cytosolic proteins targeted for degradation are tagged with a chaperone and are directly transferred into and degraded in a late endosomal compartment.
late endosome to lysosome transport via multivesicular body sorting pathway	The directed movement of substances from late endosomes to lysosomes by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the lysosome.
macroautophagy	The major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane-bounded autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane-bounded structure. Autophagosomes then fuse with a lysosome (or vacuole) releasing single-membrane-bounded autophagic bodies that are then degraded within the lysosome (or vacuole). Some types of macroautophagy, e.g. pexophagy, mitophagy, involve selective targeting of the targets to be degraded.
membrane fission	A process that is carried out at the cellular level which results in the separation of a single continuous membrane into two membranes.
midbody abscission	The process by which the midbody, the cytoplasmic bridge that connects the two prospective daughter cells, is severed at the end of mitotic cytokinesis, resulting in two separate daughter cells.
mitotic metaphase plate congression	The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis.
multivesicular body assembly	The aggregation, arrangement and bonding together of a set of components to form a multivesicular body, a type of late endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
multivesicular body sorting pathway	A vesicle-mediated transport process in which transmembrane proteins are ubiquitylated to facilitate their entry into luminal vesicles of multivesicular bodies (MVBs); upon subsequent fusion of MVBs with lysosomes or vacuoles, the cargo proteins are degraded.
negative regulation of cell death	Any process that decreases the rate or frequency of cell death. Cell death is the specific activation or halting of processes within a cell so that its vital functions markedly cease, rather than simply deteriorating gradually over time, which culminates in cell death.
negative regulation of exosomal secretion	Any process that stops, prevents or reduces the frequency, rate or extent of exosomal secretion.
nuclear membrane reassembly	The reformation of the nuclear membranes following their breakdown in the context of a normal process.
nucleus organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nucleus.
plasma membrane repair	The resealing of a cell plasma membrane after cellular wounding due to, for instance, mechanical stress.
positive regulation of centriole elongation	Any process that activates or increases the frequency, rate or extent of centriole elongation.
positive regulation of exosomal secretion	Any process that activates or increases the frequency, rate or extent of exosomal secretion.
positive regulation of G2/M transition of mitotic cell cycle	Any signalling pathway that activates or increases the activity of a cell cycle cyclin-dependent protein kinase to modulate the switch from G2 phase to M phase of the mitotic cell cycle.
potassium ion transport	The directed movement of potassium ions (K+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
protein depolymerization	The process in which protein polymers, compounds composed of a large number of component monomers, are broken down. Depolymerization occurs by the successive removal of monomers from an existing poly- or oligomeric protein.
protein transport	The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of centrosome duplication	Any process that modulates the frequency, rate or extent of centrosome duplication. Centrosome duplication is the replication of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized.
regulation of mitotic spindle assembly	Any process that modulates the frequency, rate or extent of mitotic spindle assembly.
response to lipid	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipid stimulus.
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation.
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway	The chemical reactions and pathways resulting in the breakdown of a protein or peptide, via the multivesicular body (MVB) sorting pathway; proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation. This process is independent of ubiquitination.
viral budding from plasma membrane	A viral budding that starts with formation of a membrane curvature in the host plasma membrane.
viral budding via host ESCRT complex	Viral budding which uses a host ESCRT protein complex, or complexes, to mediate the budding process.

13 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P52917	VPS4	Vacuolar protein sorting-associated protein 4	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PR
Q0VD48	VPS4B	Vacuolar protein sorting-associated protein 4B	Bos taurus (Bovine)	SS
Q9UN37	VPS4A	Vacuolar protein sorting-associated protein 4A	Homo sapiens (Human)	EV
O75449	KATNA1	Katanin p60 ATPase-containing subunit A1	Homo sapiens (Human)	PR
Q9UBP0	SPAST	Spastin	Homo sapiens (Human)	PR
A6NCM1	IQCA1L	IQ and AAA domain-containing protein 1-like	Homo sapiens (Human)	PR
Q86XH1	IQCA1	Dynein regulatory complex protein 11	Homo sapiens (Human)	PR
Q6PIW4	FIGNL1	Fidgetin-like protein 1	Homo sapiens (Human)	PR
P46467	Vps4b	Vacuolar protein sorting-associated protein 4B	Mus musculus (Mouse)	PR
Q8VEJ9	Vps4a	Vacuolar protein sorting-associated protein 4A	Mus musculus (Mouse)	PR
Q793F9	Vps4a	Vacuolar protein sorting-associated protein 4A	Rattus norvegicus (Rat)	PR
D0FH76	VPS4	Vacuolar protein sorting-associated protein 4	Bombyx mori (Silk moth)	SS
Q9ZNT0	SKD1	Protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1	Arabidopsis thaliana (Mouse-ear cress)	PR

10	20	30	40	50	60
MSSTSPNLQK	AIDLASKAAQ	EDKAGNYEEA	LQLYQHAVQY	FLHVVKYEAQ	GDKAKQSIRA
70	80	90	100	110	120
KCTEYLDRAE	KLKEYLKNKE	KKAQKPVKEG	QPSPADEKGN	DSDGEGESDD	PEKKKLQNQL
130	140	150	160	170	180
QGAIVIERPN	VKWSDVAGLE	GAKEALKEAV	ILPIKFPHLF	TGKRTPWRGI	LLFGPPGTGK
190	200	210	220	230	240
SYLAKAVATE	ANNSTFFSIS	SSDLVSKWLG	ESEKLVKNLF	QLARENKPSI	IFIDEIDSLC
250	260	270	280	290	300
GSRSENESEA	ARRIKTEFLV	QMQGVGVDND	GILVLGATNI	PWVLDSAIRR	RFEKRIYIPL
310	320	330	340	350	360
PEPHARAAMF	KLHLGTTQNS	LTEADFRELG	RKTDGYSGAD	ISIIVRDALM	QPVRKVQSAT
370	380	390	400	410	420
HFKKVRGPSR	ADPNHLVDDL	LTPCSPGDPG	AIEMTWMDVP	GDKLLEPVVS	MSDMLRSLSN
430	440
TKPTVNEHDL	LKLKKFTEDF	GQEG