O70589

SS
Gene name
Cask (Lin-2)
Protein name
Peripheral plasma membrane protein CASK
Names
EC 2.7.11.1 , Calcium/calmodulin-dependent serine protein kinase
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:12361
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

CaMKII is one of the most important transducers of Ca2+signals in a variety of cell types and is highly conserved across animal species. The calmodulin-responsive regulatory segment forms a coiled coil structure that blocks peptide and ATP binding to the otherwise intrinsically active kinase domain.Ca2+/CaM removes the autoinhibitory regulatory segment located C-terminal to the kinase domain which releases the catalytic activity of the enzyme.

Autoinhibitory domains (AIDs)

280-324 (Regulatory segment) SS

Target domain

12-276 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

161-184 (Activation loop from InterPro)

Target domain

12-276 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

3 structures for O70589

Entry ID Method Resolution Chain Position Source
1Y74 NMR - B/D 405-454 PDB
6Y9O X-ray 163 A C 915-926 PDB
AF-O70589-F1 Predicted AlphaFoldDB

No variants for O70589

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O70589

No associated diseases with O70589

11 regional properties for O70589

Type Name Position InterPro Accession
domain Protein kinase domain 12 - 276 IPR000719
domain SH3 domain 612 - 682 IPR001452
domain PDZ domain 488 - 571 IPR001478
domain L27 domain 343 - 401 IPR004172-1
domain L27 domain 402 - 458 IPR004172-2
domain Guanylate kinase-like domain 739 - 911 IPR008144
domain Guanylate kinase/L-type calcium channel beta subunit 738 - 914 IPR008145
domain L27 domain, C-terminal 347 - 395 IPR014775-1
domain L27 domain, C-terminal 406 - 455 IPR014775-2
conserved_site Guanylate kinase, conserved site 774 - 791 IPR020590
domain CASK, SH3 domain 616 - 677 IPR035473

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Cell membrane ; Peripheral membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

23 GO annotations of cellular component

Name Definition
apical dendrite A dendrite that emerges near the apical pole of a neuron. In bipolar neurons, apical dendrites are located on the opposite side of the soma from the axon.
basement membrane A collagen-containing extracellular matrix consisting of a thin layer of dense material found in various animal tissues interposed between the cells and the adjacent connective tissue. It consists of the basal lamina plus an associated layer of reticulin fibers.
basolateral plasma membrane The region of the plasma membrane that includes the basal end and sides of the cell. Often used in reference to animal polarized epithelial membranes, where the basal membrane is the part attached to the extracellular matrix, or in plant cells, where the basal membrane is defined with respect to the zygotic axis.
cell-cell junction A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects.
ciliary membrane The portion of the plasma membrane surrounding a cilium.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
nuclear lamina The fibrous, electron-dense layer lying on the nucleoplasmic side of the inner membrane of a cell nucleus, composed of lamin filaments. The polypeptides of the lamina are thought to be concerned in the dissolution of the nuclear envelope and its re-formation during mitosis. The lamina is composed of lamin A and lamin C filaments cross-linked into an orthogonal lattice, which is attached via lamin B to the inner nuclear membrane through interactions with a lamin B receptor, an IFAP, in the membrane.
nuclear matrix The dense fibrillar network lying on the inner side of the nuclear membrane.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
podocyte foot A cell projection of a podocyte (glomerular visceral epithelial cell) forming a foot-like structure projecting from a podocyte primary projection, that wraps around capillaries of a renal glomerulus. Adjacent feet (pedicels) interdigitate, leaving thin filtration slits between them, which are covered by slit diaphragms.
presynaptic membrane A specialized area of membrane of the axon terminal that faces the plasma membrane of the neuron or muscle fiber with which the axon terminal establishes a synaptic junction; many synaptic junctions exhibit structural presynaptic characteristics, such as conical, electron-dense internal protrusions, that distinguish it from the remainder of the axon plasma membrane.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
Schaffer collateral - CA1 synapse A synapse between the Schaffer collateral axon of a CA3 pyramidal cell and a CA1 pyramidal cell.
synapse The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane.
synaptic membrane A specialized area of membrane on either the presynaptic or the postsynaptic side of a synapse, the junction between a nerve fiber of one neuron and another neuron or muscle fiber or glial cell.
vesicle Any small, fluid-filled, spherical organelle enclosed by membrane.

9 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
neurexin family protein binding Binding to a neurexin, a synaptic cell surface protein related to latrotoxin receptor, laminin and agrin. Neurexins act as cell recognition molecules at nerve terminals.
PDZ domain binding Binding to a PDZ domain of a protein, a domain found in diverse signaling proteins.
protein kinase C binding Binding to protein kinase C.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
protein-containing complex binding Binding to a macromolecular complex.
signaling receptor binding Binding to one or more specific sites on a receptor molecule, a macromolecule that undergoes combination with a hormone, neurotransmitter, drug or intracellular messenger to initiate a change in cell function.

15 GO annotations of biological process

Name Definition
calcium ion import The directed movement of calcium ions into a cell or organelle.
establishment of localization in cell Any process, occuring in a cell, that localizes a substance or cellular component. This may occur via movement, tethering or selective degradation.
negative regulation of cell-matrix adhesion Any process that stops, prevents, or reduces the rate or extent of cell adhesion to the extracellular matrix.
negative regulation of cellular response to growth factor stimulus Any process that decreases the rate, frequency, or extent of a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a growth factor stimulus.
negative regulation of keratinocyte proliferation Any process that decreases the rate, frequency or extent of keratinocyte proliferation. Keratinocyte proliferation is the multiplication or reproduction of keratinocytes, resulting in the expansion of a cell population.
negative regulation of wound healing Any process that decreases the rate, frequency, or extent of the series of events that restore integrity to a damaged tissue, following an injury.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of calcium ion import Any process that increases the rate, frequency, or extent of the directed movement of calcium ions into a cell or organelle.
positive regulation of dendritic spine morphogenesis Any process that increases the rate, frequency, or extent of dendritic spine morphogenesis, the process in which the anatomical structures of a dendritic spine are generated and organized. A dendritic spine is a protrusion from a dendrite and a specialized subcellular compartment involved in synaptic transmission.
positive regulation of insulin secretion Any process that activates or increases the frequency, rate or extent of the regulated release of insulin.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
protein localization to plasma membrane A process in which a protein is transported to, or maintained in, a specific location in the plasma membrane.
regulation of neurotransmitter secretion Any process that modulates the frequency, rate or extent of the regulated release of a neurotransmitter from a cell.
regulation of synaptic vesicle exocytosis Any process that modulates the frequency, rate or extent of synaptic vesicle exocytosis.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.

33 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3MHJ9 CAMK2B Calcium/calmodulin-dependent protein kinase type II subunit beta Bos taurus (Bovine) SS
Q2HJF7 CAMK2D Calcium/calmodulin-dependent protein kinase type II subunit delta Bos taurus (Bovine) SS
Q5ZKI0 CAMK2D Calcium/calmodulin-dependent protein kinase type II delta chain Gallus gallus (Chicken) SS
Q00168 CaMKII Calcium/calmodulin-dependent protein kinase type II alpha chain Drosophila melanogaster (Fruit fly) SS
Q24210 CASK Peripheral plasma membrane protein CASK Drosophila melanogaster (Fruit fly) SS
Q9NZW5 PALS2 Protein PALS2 Homo sapiens (Human) PR
Q00013 MPP1 55 kDa erythrocyte membrane protein Homo sapiens (Human) PR
Q96JB8 MPP4 MAGUK p55 subfamily member 4 Homo sapiens (Human) PR
Q14168 MPP2 MAGUK p55 subfamily member 2 Homo sapiens (Human) PR
Q13555 CAMK2G Calcium/calmodulin-dependent protein kinase type II subunit gamma Homo sapiens (Human) EV
Q13557 CAMK2D Calcium/calmodulin-dependent protein kinase type II subunit delta Homo sapiens (Human) EV
Q13554 CAMK2B Calcium/calmodulin-dependent protein kinase type II subunit beta Homo sapiens (Human) EV
Q9UQM7 CAMK2A Calcium/calmodulin-dependent protein kinase type II subunit alpha Homo sapiens (Human) EV
O14936 CASK Peripheral plasma membrane protein CASK Homo sapiens (Human) EV
Q923T9 Camk2g Calcium/calmodulin-dependent protein kinase type II subunit gamma Mus musculus (Mouse) SS
P11798 Camk2a Calcium/calmodulin-dependent protein kinase type II subunit alpha Mus musculus (Mouse) PR
Q6PHZ2 Camk2d Calcium/calmodulin-dependent protein kinase type II subunit delta Mus musculus (Mouse) SS
P28652 Camk2b Calcium/calmodulin-dependent protein kinase type II subunit beta Mus musculus (Mouse) SS
P70290 Mpp1 55 kDa erythrocyte membrane protein Mus musculus (Mouse) PR
Q9JLB0 Pals2 Protein PALS2 Mus musculus (Mouse) PR
Q9WV34 Mpp2 MAGUK p55 subfamily member 2 Mus musculus (Mouse) PR
Q6P7F1 Mpp4 MAGUK p55 subfamily member 4 Mus musculus (Mouse) PR
Q8BVD5 Mpp7 MAGUK p55 subfamily member 7 Mus musculus (Mouse) PR
O88910 Mpp3 MAGUK p55 subfamily member 3 Mus musculus (Mouse) PR
Q95266 CAMK2D Calcium/calmodulin-dependent protein kinase type II subunit delta Sus scrofa (Pig) SS
Q9QYH1 Mpp4 MAGUK p55 subfamily member 4 Rattus norvegicus (Rat) PR
P15791 Camk2d Calcium/calmodulin-dependent protein kinase type II subunit delta Rattus norvegicus (Rat) SS
P11275 Camk2a Calcium/calmodulin-dependent protein kinase type II subunit alpha Rattus norvegicus (Rat) PR
P11730 Camk2g Calcium/calmodulin-dependent protein kinase type II subunit gamma Rattus norvegicus (Rat) SS
O62305 unc-43 Calcium/calmodulin-dependent protein kinase type II Caenorhabditis elegans EV
P54936 lin-2 Protein lin-2 Caenorhabditis elegans SS
Q6DGS3 camk2d2 Calcium/calmodulin-dependent protein kinase type II delta 2 chain Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q6DEH3 camk2d1 Calcium/calmodulin-dependent protein kinase type II delta 1 chain Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MADDDVLFED VYELCEVIGK GPFSVVRRCI NRETGQQFAV KIVDVAKFTS SPGLSTEDLK
70 80 90 100 110 120
REASICHMLK HPHIVELLET YSSDGMLYMV FEFMDGADLC FEIVKRADAG FVYSEAVASH
130 140 150 160 170 180
YMRQILEALR YCHDNNIIHR DVKPHCVLLA SKENSAPVKL GGFGVAIQLG ESGLVAGGRV
190 200 210 220 230 240
GTPHFMAPEV VKREPYGKPV DVWGCGVILF ILLSGCLPFY GTKERLFEGI IKGKYKMNPR
250 260 270 280 290 300
QWSHISESAK DLVRRMLMLD PAERITVYEA LNHPWLKERD RYAYKIHLPE TVEQLRKFNA
310 320 330 340 350 360
RRKLKGAVLA AVSSHKFNSF YGDPPEELPD FSEDPTSSGL LAAERAVSQV LDSLEEIHAL
370 380 390 400 410 420
TDCSEKDLDF LHSVFQDQHL HTLLDLYDKI NTKSSPQIRN PPSDAVQRAK EVLEEISCYP
430 440 450 460 470 480
ENNDAKELKR ILTQPHFMAL LQTHDVVAHE VYSDEALRVT PPPTSPYLNG DSPESANGDM
490 500 510 520 530 540
DMENVTRVRL VQFQKNTDEP MGITLKMNEL NHCIVARIMH GGMIHRQGTL HVGDEIREIN
550 560 570 580 590 600
GISVANQTVE QLQKMLREMR GSITFKIVPS YRTQSSSCER DSPSTSRQSP ANGHSSTNNS
610 620 630 640 650 660
VSDLPSTTQP KGRQIYVRAQ FEYDPAKDDL IPCKEAGIRF RVGDIIQIIS KDDHNWWQGK
670 680 690 700 710 720
LENSKNGTAG LIPSPELQEW RVACIAMEKT KQEQQASCTW FGKKKKQYKD KYLAKHNAVF
730 740 750 760 770 780
DQLDLVTYEE VVKLPAFKRK TLVLLGAHGV GRRHIKNTLI TKHPDRFAYP IPHTTRPPKK
790 800 810 820 830 840
DEENGKNYYF VSHDQMMQDI SNNEYLEYGS HEDAMYGTKL ETIRKIHEQG LIAILDVEPQ
850 860 870 880 890 900
ALKVLRTAEF APFVVFIAAP TITPGLNEDE SLQRLQKESD VLQRTYAHYF DLTIINNEID
910 920
ETIRHLEEAV ELVCTAPQWV PVSWVY